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An analytical method without the need for derivatization was established in this study, employing dispersive solid-phase extraction (d-SPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the direct determination of residues of glufosinate, glyphosate and their metabolites (NAG, MPPA, AMPA) in tea and Zanthoxylum bungeanum. After extraction with 10 mL of deionized water and 10 mL of 1% formic acid-methanol solution, samples were processed using d-SPE with composite multi-walled carbon nanotubes (C-MWCNTs) as the core purification material to enhance the separation efficiency between the target compounds and the matrix and to reduce matrix interference. Finally, detection of five target compounds was achieved using LC-MS/MS. The mobile phases were water with 0.7% formic acid and acetonitrile with 0.7% formic acid. The separation was performed on an anionic polar column (5 μm, 2.1 mm × 100 mm) at 40 ℃. The final extracts were detected using the dynamic multiple reaction monitoring (dMRM) method in negative mode and were quantified using external matrix-matched calibration curves. The results showed that the five analytes had sharp peak shapes and good separation under the instrument conditions, and the linearity was good in the range of 0.01-2 μg/mL. The coefficients of determination (R²) were higher than 0.9987 and 0.9992 for tea and Z. bungeanum, respectively. The limits of quantification (LOQ) of the five compounds in tea and Z.bungeanum were 0.03-0.10 mg/kg and 0.03-0.16 mg/kg, respectively. At three spiked levels of 0.25, 0.50 and 1.0 mg/kg, the average recoveries of glufosinate, glyphosate and their metabolites in tea (Tibetan tea) ranged from 61% to 105% with relative standard deviations (RSD) from 0.22% to 13%, and were 78%-115% in Z. bungeanum (red Z. bungeanum) with RSD of 0.12%-7.5%. The sample preparation process of this method does not require derivatization, the cleanup steps are simple, the instrumental running time is short, and the recoveries and precision met the requirements of method validation. It is suitable for the rapid determination of glufosinate, glyphosate and their metabolites (NAG, MPPA, AMPA) in large quantities of tea and Z. bungeanum. [ABSTRACT FROM AUTHOR]

AbstractAmaranth (Amaranthus spp.) resistant to glyphosate and diclosulam represent the most important weed complex in Uruguayan agricultural systems. The objective of this study was to evaluate the use of cover crops (CC) and herbicide strategies (HS) in the management of amaranth. The work was divided into two stages, the first conducted at the field, where the use of CC and pre/post-emergent HS was evaluated. Black oat and Rye were used as CC, and 15 HS (pre and/or post-emergent) were applied to manage amaranth in soybean crops. The second study was conducted in a greenhouse, where the control of amaranth was evaluated using 15 pre-emergent HS associated with black oat or rye straw. Nine HS were also considered for post-emergent weed management. The results showed that both at the field and greenhouse levels, the presence of 7 tons ha−1 of CC straw reduced amaranth emergence by up to 70% and did not interfere with the performance of pre-emergent herbicides. The low occurrence of Amaranthus facilitate control of HS and minimize the selection pressure for resistance exerted by herbicides on these weeds. For post-emergent weed management, the use of fomesafen + benazolin and, especially 2,4-D + ammonium glufosinate are effective options for managing resistant amaranth. [ABSTRACT FROM AUTHOR]

The establishment of industrial hemp (Cannabis sativa L.) fields near row crops has raised concerns about the potential adverse effects of herbicide drift on hemp production. This study examined hemp susceptibility to drift of herbicides registered for use in corn and/or soybeans. Herbicide solutions (2,4-D, dicamba, glufosinate, glyphosate, imazethapyr, lactofen, mesotrione) were applied separately in the wind tunnel (3.6 m s−1 airspeed), simulating drift scenarios, with conventional TP95015EVS (TP) and air inclusion AI95015EVS (AI) flat fan nozzles calibrated to deliver 140 L ha−1 carrier volume at 230 kPa. Mylar cards and hemp plants (20–25 cm tall) were placed downwind up to 12 m. Spray deposition from mylar cards was quantified using fluorometry and hemp biomass was collected 21 post application. Results indicated the nozzle design influenced downwind deposition; 5% of spray deposits from the TP nozzle reached 5.9 m downwind versus 2.0 m for the AI nozzle. Glyphosate, glufosinate, and mesotrione caused the highest biomass reductions, with 50% reductions observed at 19.3 (inferred), 8.7, and 9.3 m downwind for TP nozzle, and 4.1, 4.0, and 2.9 m for AI nozzle. These findings suggest herbicide applications at airspeeds of 3.6 m s−1 or greater present a risk to nearby hemp fields. [ABSTRACT FROM AUTHOR]

Crop resistance to herbicides is crucial for agricultural productivity and sustainability amidst escalating challenges of weed resistance. Uncovering herbicide resistant genes is particularly important for rice production. In this study, we tested the resistance to three commonly used herbicides: glufosinate, glyphosate and mesotrione of 421 diverse rice cultivars and employed genome-wide association studies (GWAS) to unravel the genetic underpinnings of resistance to these three herbicides in rice. We discovered that cultivated rice exhibited rich variation in resistance to the three herbicides, and the differences among subpopulations were significant. Six identified associations harboring candidate genes for resistance to these herbicides were significant. Among them, RGlu6 and RGly8 were the major QTL for resistance to glufosinate and glyphosate, respectively. The favorable alleles of RGlu6 and RGly8 were primarily present in japonica cultivars that originated from Europe, highlighting the geographic and genetic diversity of herbicide resistance and emphasizing the localized selection pressures in European rice varieties. Moreover, our findings might suggest that traditional target genes may not contain tolerant alleles in nature, and alternative mechanisms with novel loci associated with resistance may work. By mapping the genes for herbicide resistance, our results may help develop new strategies to combat the dual challenges on effective weed management and herbicide sustainability. [ABSTRACT FROM AUTHOR]

Crop resistance to herbicides is crucial for agricultural productivity and sustainability amidst escalating challenges of weed resistance. Uncovering herbicide resistant genes is particularly important for rice production. In this study, we tested the resistance to three commonly used herbicides: glufosinate, glyphosate and mesotrione of 421 diverse rice cultivars and employed genome-wide association studies (GWAS) to unravel the genetic underpinnings of resistance to these three herbicides in rice. We discovered that cultivated rice exhibited rich variation in resistance to the three herbicides, and the differences among subpopulations were significant. Six identified associations harboring candidate genes for resistance to these herbicides were significant. Among them, RGlu6 and RGly8 were the major QTL for resistance to glufosinate and glyphosate, respectively. The favorable alleles of RGlu6 and RGly8 were primarily present in japonica cultivars that originated from Europe, highlighting the geographic and genetic diversity of herbicide resistance and emphasizing the localized selection pressures in European rice varieties. Moreover, our findings might suggest that traditional target genes may not contain tolerant alleles in nature, and alternative mechanisms with novel loci associated with resistance may work. By mapping the genes for herbicide resistance, our results may help develop new strategies to combat the dual challenges on effective weed management and herbicide sustainability. [ABSTRACT FROM AUTHOR]

AbstractAquatic communities are increasingly exposed to complex mixtures of contaminants, mainly pesticides due to the impact of agricultural activity. The aim of this study was to evaluate the toxicity of an eight-pesticide cocktail on larvae of the South American common toad, Rinella arenarum. The cocktail represents a realistic mixture of insecticides (cypermethrin, chlorpyrifos and lambda-cyhalothrin), herbicides (glyphosate, glufosinate ammonium, prometryn and metolachlor), and a fungicide (pyraclostrobin) previously found in aquatic organisms (Prochilodus lineatus) from the Salado River Basin, an area with strong agricultural pressure. Computational simulations through the Density Functional Tight-Binding method indicated a strong spontaneous trend toward the formation of the cocktail, suggesting that it may act as a novel xenobiotic entity in the environment. The cocktail effects were evaluated in early-developing and premetamorphic larvae, at feasible concentrations found in real scenarios. The mixture led to high mortality and teratogenicity in early-developing larvae. Premetamorphic larvae showed endocrine disruption, oxidative stress, and impairments in detoxification and hepatic functioning. Neurotoxicity, genotoxicity, cardiotoxicity and high mortality under stress conditions were also observed in exposed larvae. This novel evaluation highlights the ecotoxicological risk for aquatic organisms exposed to complex mixtures and underscores the need to consider cocktail effects in studies regarding ecosystems health. [ABSTRACT FROM AUTHOR]

On-site detection of herbicides is imperative to ensure food safety; thus, effective electrochemical sensors are required. In this study, electrochemical detection of glyphosate and glufosinate was achieved through the rational design of a defect-engineered transductor based on surface-defective NiAl layered double hydroxides supported on a N-doped carbonaceous support. The NiAl atomic composition was optimized, and the 3:1 ratio exhibited the best performance. In phosphate buffer solution (0.1 M PBS pH 11), this material presented a sensitivity of 276 mA M–1 cm–2 and a limit of detection (LOD) of 0.43 μM (as determined by cyclic voltammetry). The LOD was enhanced to 0.081 μM using differential pulse voltammetry. An electrochemical solid-state sensor for on-site tests was developed using screen-printed electrodes and hydrated poly-(vinyl alcohol) membranes. The solid-state prototype was tested under laboratory conditions by spraying glyphosate onto a plant, and the electrochemical results demonstrated good repeatability and stability. The excellent detection performance of the sensor was attributed to the induced surface defects and the major center of Ni exposure, as determined by chemical calculations, where the induced defects enabled changes in the surface electrical properties of the layered double hydroxide (LDH). [ABSTRACT FROM AUTHOR]

The biocatalytic oxidative deamination‐ reductive amination process for the production of L‐phosphinothricin (L‐PPT) from D,L‐phosphinothricin (D,L‐PPT) is a green and environmentally friendly approach with significant development potential. In this study, the adopted technological route involves the oxidative deamination of the D‐PPT component in D,L‐PPT to 2‐oxo‐4‐(hydroxymethylphosphinyl)butanoic acid (PPO) by utilizing D‐amino acid oxidase (DAAO), followed by the catalytic reductive amination of PPO to L‐PPT using phosphinothricin dehydrogenase (PpDH). In order to enhance the catalytic rate and reduce the inhibitory effect of the intermediate product PPO on the catalytic process, optimization of both oxidative deamination and reductive amination phases was conducted through separate strategies of constant‐pressure oxygen supply and substrate feeding, respectively. A "two‐step one‐pot" method was employed to link the optimized reactions, significantly shortening the production time. Furthermore, the "two‐step one‐pot" catalytic strategy was optimized, ultimately achieving a high 97.7% yield of L‐PPT with ee of L‐PPT>99.9% after 5.5 h of reaction under 800 mM D,L‐PPT catalytic conditions, D‐PPT and PPO could not bedetected in the reaction system, further enchanced the catalytic efficiency, production efficiency and product quality. The aforementioned results demonstrate that this proposed approach has great potential for industrial‐scale production of L‐PPT as well as significant competitive advantages in terms of economic feasibility. [ABSTRACT FROM AUTHOR]

Food insecurity which has been a global threat, forces researchers to develop crops with increased productivity even under varying climatic conditions. Rice, being a significant staple and strategic crop, helps ensure economic stability, food, and nutritional security globally. It meets 20% of the calorie requirement of people residing all over the world. Lately, rice cultivation and research have been facing hitherto unprecedented difficulties in the context of climate- induced water scarcity and dwindling resources of manpower, arable land, etc. In this regard, direct seeded rice (DSR) as a resource conservation technique is gaining popularity as a potential alternative to conventional transplanting with reduced input requirement, reduced methane and CO2 emission, increased adaptability to climate change, and increased economic returns. The weed menace in DSR prevents it from reaching its fruitful attainment to a significant level. DSR highly depends on herbicide for weed control as manual weeding and other cultural practices are labour intensive which again meets a setback of crop injury (non-selective herbicide) and resistant weeds (selective herbicides). Herbicide-tolerant (HT) rice could be an effective and long-term solution for weed management in DSR. Three HT rice systems, viz., imidazolinones, glyphosate, and glufosinate, have already been developed in this context. This review gives an insight into the need for HT rice in DSR, its production system, limitations, and stewardship guidelines for proper weed management in rice. [ABSTRACT FROM AUTHOR]

Annual ryegrass (Lolium rigidum) is a problematic weed in winter crops and fallows in the southeastern cropping region (SCR) of Australia. This weed has evolved resistance to multiple herbicide groups, globally. In Australia, L. rigidum is more prevalent in the western and southern regions than in SCR. To assess the herbicide resistance status of L. rigidum, the response of five L. rigidum populations (collected from the SCR) to glyphosate, glufosinate, paraquat, haloxyfop-P-ethyl, and clethodim is determined using dose–response curves. Three parametric logistic models are used to determine the herbicide dose required to achieve 50% survival (LD50) and 50% growth reduction (GR50). The LD50 values for 50% survival at 28 days after treatment range from 1702 g a.e. ha−1 to 8225 g a.e. ha−1 for glyphosate, 1637 g a.i. ha−1 to 1828 g a.i. ha−1 for glufosinate, 141 g a.i. ha−1 to 307 g a.i. ha−1 for paraquat, 11 g a.i. ha−1 to 107 g a.i. ha−1 for haloxyfop-P-ethyl, and 17 g a.i. ha−1 to 48 g a.i. ha−1 for clethodim. The resistance factor, based on GR50 value, is highest in the S7 population (2.2 times) for glyphosate, the S11 population (2.3 times) for glufosinate, the S11 population (2.0 time) for paraquat, the S7 population (3.9 times) for haloxyfop-P-ethyl, and the S3 population (3.1 times) for clethodim, compared with the susceptible or less tolerant population. The S11 population is found to be resistant to five tested herbicides, based on resistance factors. Similarly, the S3 population is highly resistant to glyphosate, haloxyfop-P-ethyl, and clethodim compared with the W4 population. These results suggest that L. rigidum populations in the SCR exhibit resistance to multiple herbicide groups at labelled field rates. The findings highlight the necessity of adopting an integrated management approach, including the use of residual herbicides, tank mixing herbicides with different modes of action, and rotating herbicides in conjunction with cultural and mechanical control methods. [ABSTRACT FROM AUTHOR]

Weeds are among the most significant factors contributing to decreases in crop yield and quality. Glufosinate, a nonselective, broad‐spectrum herbicide, has been extensively utilized for weed control in recent decades. However, crops are usually sensitive to glufosinate. Therefore, the development of glufosinate‐resistant crops is crucial for effective weed management in agriculture. In this study, we characterized a SQUAMOSA promoter‐binding‐like (SPL) factor, OsSPL8, which acts as a negative regulator of glufosinate resistance by inhibiting the transcription of OsGS1;1 and OsGS2 and consequently reducing GS activity. Furthermore, the loss of OsSPL8 function enhanced tolerance to drought and salt stresses. Transcriptomic comparisons between the gar18‐3 mutant and wild type revealed that OsSPL8 largely downregulates stress‐responsive genes and upregulates growth‐related genes. We demonstrated that OsSPL8 directly regulates OsOMTN6 and OsNAC17, which influence drought tolerance. In addition, OsSPL8 directly represses the expression of salt stress tolerance‐related genes such as OsHKT1.1 and OsTPP1. Collectively, our results demonstrate that OsSPL8 is a negative regulator of both glufosinate resistance and abiotic stress tolerance. Summary statement: This study revealed that OsSPL8 acts as a negative regulator of glufosinate resistance in rice by directly inhibiting the transcription of GLUTAMINE SYNTHETASE 1;1 (OsGS1;1) and GLUTAMINE SYNTHETASE 2 (OsGS2). Meanwhile, loss of OsSPL8 function enhances tolerance to drought and salinity stress. [ABSTRACT FROM AUTHOR]

Chemical desiccation is widely used in agriculture to anticipate harvest and mitigate the effects of adverse environmental conditions. It is applied to both grains and seeds. Although this practice is widely used, there are still significant gaps in understanding the effects of different herbicide application times on seed quality and plant physiological responses. The objective of this study was to evaluate the effects of different herbicide application times on cowpea, focusing on seed quality, physiological responses, and biochemical composition, including chlorophylls, carotenoids, sugars, and proline, under nocturnal desiccation. In the first experiment, eight herbicides and two mixtures were applied at night: diquat, flumioxazin, diquat + flumioxazin, glufosinate ammonium, saflufenacil, carfentrazone, diquat + carfentrazone, atrazine, and glyphosate. All of the tested herbicides caused a reduction in normal seedling formation, with the diquat + carfentrazone combination resulting in 100% abnormal seedlings. A significant decrease in chlorophyll levels (chlorophyll a: 63.5%, chlorophyll b: 50.2%) was observed using diquat, which indicates damage to photosynthetic processes, while the carotenoid content increased. Total soluble sugars and proline were also negatively impacted, reflecting physiological stress and metabolic changes in seedlings. In the second experiment, three application times were tested with diquat, diquat + flumioxazin, and diquat + carfentrazone. Nocturnal application showed the most significant reduction in chlorophyll levels and increased carotenoid levels. Application at noon and late afternoon also significantly changed the soluble sugar and proline levels. These results indicate that the herbicide application time directly influences the seeds' physiological quality. [ABSTRACT FROM AUTHOR]

AbstractItalian ryegrass stands out among the most problematic weeds injuring wheat crops. This study aimed to evaluate the efficacy of herbicides in controlling Italian ryegrass. Six field experiments were conducted under a randomized block design with a split-plot arrangement. The contact herbicides glufosinate or diquat performed better when sequentially applied after ACCase-inhibiting herbicides. Glufosinate had a slower effect than diquat. However, glufosinate was more efficacious in all studies. Adding the PROTOX-inhibiting herbicides carfentrazone or saflufenacil did not improve the efficacy of glufosinate. The mixture of glyphosate or glufosinate with ACCase-inhibitor herbicides resulted in an additive effect. The mix of glufosinate + diquat or clethodim + diquat had an antagonistic effect. The glufosinate + diquat mixture resulted in 20% less control than expected. The dose increase was significant only for glufosinate and not for diquat. Starting at a dose of 560 g ha−1 glufosinate, 26 g ha−1 was needed to increase control by 1%. The present study concludes that glufosinate should be used as the main alternative to paraquat for controlling Italian ryegrass. [ABSTRACT FROM AUTHOR]

Multiple herbicide-resistant kochia (Bassia scoparia (L.) A.J. Scott) has grown in prevalence in the canola (Brassica napus L.) production region of North America. Glufosinate-resistant canola facilitates kochia management since glufosinate-resistant kochia is not known to occur. Field experiments were conducted from 2013 to 2015 in five environments near Lethbridge and Coalhurst, Alberta, to identify herbicide strategies targeting acetolactate synthase inhibitor-resistant kochia with and without glyphosate resistance in glufosinate-resistant canola. Sequential glufosinate treatments (500 g ai ha−1) postemergence (POST) caused excellent (≥90%) kochia control and biomass reduction across environments. Preplant (PP) carfentrazone + sulfentrazone (9 + 105 g ai ha−1) alone or followed by (fb) POST glufosinate (9 + 27 fb 500 or 9 + 105 fb 500 g ai ha−1) resulted in excellent kochia control in all environments tested. PP carfentrazone + sulfentrazone (9 + 53 g ai ha−1) alone, and a single POST treatment with glufosinate (500 or 590 g ai ha−1) alone or preceded by fall-applied ethalfluralin (1100 fb 500 g ai ha−1) with or without PP carfentrazone (1100 fb 9 fb 500 g ai ha−1) caused ≥80% kochia control and biomass reduction in all environments tested. However, treatments containing PP carfentrazone + sulfentrazone caused unacceptable canola injury or yield loss in at least one environment. In conclusion, single or sequential treatments of glufosinate POST managed multiple herbicide-resistant kochia effectively in canola. Layering fall-applied/PP ethalfluralin and/or PP carfentrazone with glufosinate POST may help alleviate resistance selection pressure placed on glufosinate in canola. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the effectiveness of synthetic auxins in the control of Conyza sumatrensis in mixtures for use in the first application, and the effectiveness of burndown herbicides alone or in mixtures for use in the second application, in soybean pre-sowing. Experiment 1 consisted of auxin herbicides (fluroxypyr or dicamba) in mixtures with herbicides (glyphosate, glufosinate, saflufenacil, carfentrazone, and chlorimuron) used for the first application in pre-sowing. Experiment 2 consisted of burndown herbicides (diquat, glufosinate, glyphosate, chlorimuron, saflufenacil, and carfentrazone), alone or at mixtures, used for sequential application in pre-sowing. For the first application, the effectiveness of glyphosate + dicamba or fluroxypyr was low and must be complemented by the addition of a PPO-inhibiting herbicide, mainly saflufenacil. The mixture of glufosinate, instead of glyphosate, with synthetic auxin was more effective, even increasing with the addition of saflufenacil. For the second application, with burndown herbicides, glufosinate was superior and effective when mixed with saflufenacil. For the first application in soybean pre-sowing, glyphosate or glufosinate + synthetic auxin + saflufenacil are effective for the control of C. sumatrensis. For the second application, glufosinate at mixtures is effective in controlling C. sumatrensis. [ABSTRACT FROM AUTHOR]

A putative glufosinate‐resistant Amaranthus palmeri population was reported in 2015 in Anson County, North Carolina. The results from dose–response assays conducted in the field suggested plants were surviving lethal rates of glufosinate. Dose–response assays conducted in the glasshouse determined the Anson County accession exhibited reduced susceptibility to glufosinate compared to three glufosinate‐susceptible populations. The LD50 values (210–316 g ai ha−1) for the Anson County population were always higher than the LD50 values (118–158 g ai ha−1) for the tested susceptible populations from the dose–response assays. Anson County plants that survived lethal glufosinate rates were reciprocally crossed with susceptible plants to create F1 genotypes and treated with a lethal rate of glufosinate (267 g ai ha−1; ascertained from glasshouse dose–response assay) to determine the distribution of injury and survival for each cross compared to a cross of susceptible parents. The distribution of injury was non‐normal for the crosses containing an Anson County plant compared to the cross with a susceptible parent. Survival was 68%–84% for crosses containing an Anson County plant, whereas the survival was significantly reduced to 35% for the susceptible plant cross. Chi‐square goodness of fit tests were used to test inheritance models to describe the responses of the genotypes. The resistant × susceptible crosses were best described with a heterozygous two loci with incomplete dominance model compared to the resistant × resistant cross that was best described with a heterozygous single locus with incomplete dominance model. The Anson County population has evolved resistance to glufosinate that is heritable and likely conferred by an oligogenic mechanism with incomplete dominance. [ABSTRACT FROM AUTHOR]

Desiccation of potato crops using herbicides is one of the methods widely used by farmers. Desiccation affects several key aspects such as tuber yield and potato skin hardness. This study aimed to investigate the effects of desiccation on potato (Solanum tuberosum L.) on skin hardness and yield. Experimental methods were used by varying the level of desiccation on different herbicide groups. Ammonium glufosinate, glyphosate, paraquat and saflufenacil herbicides were used in this study which were then compared with manual trimming and no desiccation treatment. The results showed that all herbicides reached a percentage of desiccation above 95% on the 15 days after application. All herbicide treatments other than glyphosate showed the same potato skin hardness as the manual trimming treatment and were harder than potato tubers without desiccation treatment. Desiccation using ammonium glufosinate herbicide at 375 g a.i./ ha had the highest potato tuber yield compared to other herbicide applications. [ABSTRACT FROM AUTHOR]

Tahitian bridal veil (Gibasis pellucida) and small-leaf spiderwort (Tradescantia fluminensis) are both invasive species in natural areas throughout Florida. However, very little is known regarding herbicide control. To provide land managers with herbicidal control options for both species, postemergence herbicides were evaluated for efficacy in a greenhouse to identify herbicide options that control both species under similar settings. Four herbicides, including triclopyr acid, triclopyr amine + 2,4-D amine, triclopyr amine, and glufosinate were applied at standard label rates and compared to a non-treated control group for efficacy. Visual control ratings were taken at 2, 4, and 8 weeks after treatment (WAT), and shoot dry weights (WAT 8) and regrowth dry weights (WAT 12) were determined. Triclopyr (acid and amine) generally provided the most consistent control of both species as evidenced by the visual control ratings and shoot dry weight data which showed reductions of 76% to 89% in shoot biomass at trial conclusion. Triclopyr + 2,4-D reduced shoot dry weights by 52% to 54% and was the least effective when considering the control of both species. [ABSTRACT FROM AUTHOR]

Diquat (DQ), paraquat (PQ), glufosinate (GLU), and glyphosate (GLYP) are commonly used herbicides that have been confirmed to be toxic to humans. Rapid and accurate measurements of these toxicants in clinical practice are beneficial for the correct diagnosis and timely treatment of herbicide-poisoned patients. The present study aimed to establish an efficient, convenient, and reliable method to achieve the simultaneous quantification of DQ, PQ, GLU, and GLYP in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) without using derivatization or ion-pairing reagents. DQ, PQ, GLU, and GLYP were extracted by the rapid protein precipitation and liquid-liquid extraction method and then separated and detected by LC-MS/MS. Subsequently, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, extraction recovery, matrix effect, dilution integrity, and stability were evaluated to validate the method based on the FDA criteria. Finally, the validated method was applied to real plasma samples collected from 166 Chinese patients with herbicide poisoning. The results showed satisfactory linearity with low LOD (1 ng/mL for DQ and PQ, 5 ng/mL for GLU, and 10 ng/mL for GLYP, respectively) and low LOQ (5 ng/mL for DQ and PQ, 25 ng/mL for GLU and GLYP, respectively). In addition, the precision, accuracy, extraction recovery, and stability of the method were acceptable. The matrix effect was not observed in the analyzed samples. Moreover, the developed method was successfully applied to determine the target compounds in real plasma samples. These data provided reliable evidence for the application of this LC-MS/MS method for clinical poisoning detection. [ABSTRACT FROM AUTHOR]

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It has been difficult to control Eleusine indica (L.) Gaertn and Spermacocea latifolia Aubl. in cotton and soybean crops due to their herbicide resistance or tolerance to glyphosate. Thus, this study's objective was to evaluate the herbicide efficacy applied in pre-emergence, associated with desiccant herbicide in sequential application, in these species. The experiments for S. latifolia and E. indica were conducted separately in a greenhouse. The experimental design was completely randomized, with 4 replications, in a 6 x 3 factorial. The first factor consisted of pre-emerging herbicides applied two days after the sowing of each species, diclosulam (35 g a.i./ha), s-metolachlor (600 g a.i./ha), pyroxasulfone (200 g a.i./ha) + flumioxazin (200 g a.i./ha), diuron (2000 g a.i./ha), trifluralin (600 g a.i./ha), and the treatment without herbicide. The second factor consisted of the application of diquat (200 g a.i./ha), ammonium glufosinate (400 g a.i./ha), or the absence of sequential application. The results revealed that only for the diclosulam herbicide a sequential application with desiccants was necessary for an effective control of E. indica. The other herbicide applied in pre-emergence showed high efficacy (> 90%) in E. indica control when applied individually. Regarding S. latifolia chemical control, the herbicides s-metolachlor (80.04%), pyroxasulfone + flumioxazin (100%), diclosulam (82.08%) and diuron (89.58%), applied in pre-emergence, presented a satisfactory control at 42 days after emergence. The sequential applications promoted better control of S. latifolia to trifluralin and diuron. In conclusion, there are pre-emergence options for the effective control of E. indica and S. latifolia. However, sequential application can be an important tool for improving the control of some weed species, such as those studied in this work, by certain herbicides. [ABSTRACT FROM AUTHOR]

Diquat, glufosinate and the combination of the latter with carfentrazone or saflufenacil are believed to be as effective for pre-harvest desiccation of soybean plants as paraquat. This work was developed with the objective of evaluating the efficacy of paraquat, diquat, and glufosinate alone or at mixtures of carfentrazone or saflufenacil in pre-harvest desiccation of soybean. Soybean defoliation, maturity and yield were evaluated. Similar efficacy was observed between glufosinate and paraquat in soybean defoliation and maturity, in addition to a greater flexibility of use, in which it could be an anticipator and standardizing agent of the harvest. The application of diquat (200 g of active ingredient [a.i.] ha-1 ), paraquat (200 g a.i. ha-1 ) or glufosinate (400 or 500 g a.i. ha-1 ) was effective for pre-harvest desiccation of soybean at the R7.2 stage. As well, it was observed promising results for application of glufosinate + saflufenacil (300 + 24.5 g a.i. ha-1 ), glufosinate + carfentrazone (200 + 25 g a.i. ha-1 ), glufosinate + saflufenacil (200 + 35 g a.i. ha-1 ). Diquat and glufosinate can replace paraquat in pre-harvest desiccation of soybean plants, and glufosinate also provide greater management flexibility, in anticipating the harvest. [ABSTRACT FROM AUTHOR]

Glyphosate has played an important role in agricultural production systems, especially after the release of glyphosate resistant crops. With increased usage and an overall reliance on chemical control, weed resistance to glyphosate has occurred and is now a major issue. The objective of this research was to investigate weed control levels provided by glufosinate, 2,4-D, and clethodim as an alternative to glyphosate. Multiple POST applications generally provided superior weed control in comparison to a single early-POST application. No programs provided greater than 80% warm-season grass control beginning 21 d after mid-POST application. Applications of glufosinate or glufosinate + 2,4-D fb clethodim + glufosinate, glufosinate + 2,4-D, or clethodim + glufosinate + 2,4-D provided adequate broadleaf weed control throughout the rating period. Although POST-only programs are an option, they are not a sustainable weed control practice. It remains important to incorporate residual herbicides into a weed control program as well as alternative weed control methods. [ABSTRACT FROM AUTHOR]

Diquat, glufosinate and the combination of the latter with carfentrazone or saflufenacil are believed to be as effective for pre-harvest desiccation of soybean plants as paraquat. This work was developed with the objective of evaluating the efficacy of paraquat, diquat, and glufosinate alone or at mixtures of carfentrazone or saflufenacil in pre-harvest desiccation of soybean. Soybean defoliation, maturity and yield were evaluated. Similar efficacy was observed between glufosinate and paraquat in soybean defoliation and maturity, in addition to a greater flexibility of use, in which it could be an anticipator and standardizing agent of the harvest. The application of diquat (200 g of active ingredient [a.i.] ha-1), paraquat (200 g a.i. ha-1) or glufosinate (400 or 500 g a.i. ha-1) was effective for pre-harvest desiccation of soybean at the R7.2 stage. As well, it was observed promising results for application of glufosinate + saflufenacil (300 + 24.5 g a.i. ha-1), glufosinate + carfentrazone (200 + 25 g a.i. ha-1), glufosinate + saflufenacil (200 + 35 g a.i. ha-1). Diquat and glufosinate can replace paraquat in pre-harvest desiccation of soybean plants, and glufosinate also provide greater management flexibility, in anticipating the harvest.

Abstract The objective of this work was to evaluate the effectiveness of herbicides when applied alone and in combinations at sourgrass (Digitaria insularis) post-emergence, as well as to identify a substitute to paraquat in sequential application. Field and greenhouse experiments were conducted during the 2019/2020 and 2020/2021 crop seasons. The herbicides applied alone and in combinations were: atrazine, clethodim, clodinafop, diquat, glufosinate, haloxyfop, imazapic, imazapyr, mesotrione, nicosulfuron, paraquat, glyphosate, saflufenacil, tembotrione, and tepraloxydim. In the 2019/2020 crop season, in the experiment conducted in the field, the control of sourgrass was considered low due to the dry weather condition and to the full flowering of the plants. In the greenhouse, a satisfactory control above 80% was observed at 28 days after herbicide application for most treatments. In the 2020/2021 crop season, under field conditions, the application of glyphosate combined with haloxyfop, with a sequential application of glufosinate, resulted in the highest weed control. In the greenhouse, most treatments were effective and, of these, all contained glufosinate. The sequential application of glufosinate or in combinations favors a better control of sourgrass. However, diquat and glufosinate do not differ in efficacy in sequential application and are an option for the control of the weed.

The abuse of herbicides has emerged as a great threat to food security. Herein, a low-background interference detection method based on UPLC-MS was developed for the simultaneous determination of glufosinate, glyphosate, and its metabolite aminomethylphosphonic acid (AMPA) in foods. Initially, this study proposed a simple and rapid pretreatment method, utilizing water extraction and PRiME HLB purification to isolate glyphosate, glufosinate, and AMPA from food samples. After the optimization of pretreatment conditions, the processed samples are then analyzed directly by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS) without pre-column derivatization. The method can effectively reduce interference from by-products of pre-column derivatization and background substrates of food sample, showing low matrix effects (ME) ranging from − 24.83 to 32.10%. Subsequently, the method has been validated by 13 kinds of food samples. The recoveries of the three herbicides in the food samples range from 84.2 to 115.6%. The limit of detection (LOD) is lower to 0.073 mg/kg, 0.017 mg/kg, and 0.037 mg/kg, respectively. Moreover, the method shows an excellent reproducibility with relative standard deviations (RSD) within 16.9%. Thus, the method can provide high trueness, reproducibility, sensitivity, and interference-free detection to ensure human health safety. Low-background interference detection of glyphosate, glufosinate and AMPA in foods based on UPLC-MS without derivatization [ABSTRACT FROM AUTHOR]

Glyphosate and glufosinate are among the most widely used pesticides in agriculture worldwide. Their extensive use leads to the presence of their residues on crops and in the surrounding environment. Beehives, bees, and apiculture products can represent potential sources for the accumulation of these substances and their metabolites, and the consequences for bee health, as well as the level of risk to human health from consuming contaminated food, are still unclear. Furthermore, information on the contamination levels of honey and other beehive products by these compounds remains poorly documented. This study is part of a broader research effort aimed at developing specific analytical methods for monitoring the level of these contaminants in bee products. The methodology employed enabled the acquisition of preliminary information concerning the levels of glyphosate and glufosinate contamination in honey samples obtained from various retailers in Italy to assess compliance with the limits established by Regulation 293/2013. The liquid chromatography tandem mass spectrometry analysis of the 30 honey samples revealed quantifiable levels of glyphosate in eight samples, with contamination ranging from 5.4 to 138.5 ng/g. Notably, one sample of the wildflower type showed residue levels nearly three times the maximum residue limit. Additionally, trace levels of glyphosate contamination were detected in another ten samples. It is noteworthy that glufosinate and its metabolites were not detected in any of the analyzed samples within the established method's detection ranges. [ABSTRACT FROM AUTHOR]

In this study, the kinetics and mechanism of UV/O3 synergistic oxidative digestion of dissolved organic phosphorus (DOP) were investigated, focusing on the ozone direct oxidation and hydroxyl radical oxidation parts of glufosinate and triphenyl phosphate (TPhP). The p‐chlorobenzoic acid (p‐CBA) was selected as the probe compound, and two kinds of reaction kinetic models were proposed by competitive kinetic method with Rct according to the different scale of rate constants of hydroxyl radical oxidation. Under the condition of weakly alkaline (pH = 9.0) and weakly acidic environment (pH = 5.0), the second‐order rate constants of glufosinate and TPhP was determined indirectly to be ko3/glufosinate = (2.903 ± 0.247)M−1s−1 and ko3/TPhP = (3.307 ± 0.204) M−1s−1 by ozone direct oxidation, and k·OH/glufosinate = (1.257 ± 1.031) × 109 M−1s−1 and k·OH/TPhP = (7.120 × 108 ± 0.963) M−1s−1 by hydroxyl radical oxidation, respectively. The comparison of the contribution levels of the two parts to the digestion process showed that the contribution levels in the digestion of glufosinate and TPhP processes both the contribution of ·OH were higher than those of ozone, 86.3% and 72.6%, respectively. [ABSTRACT FROM AUTHOR]

This document discusses the hazardous effects of pesticides on human health. Pesticides are widely used to control harmful organisms in agriculture, public, and veterinary practices. However, they can also have unintended harmful effects on human health. The document highlights the diverse pathways of exposure and the complex toxicological profiles of pesticide formulations. It emphasizes the need for ongoing research due to the wide array of pesticides and the development of new ingredients and formulations. The document also explores the impact of climate change on pesticide use and the increased risks associated with occupational exposure during heat waves. Additionally, it discusses the toxicity of pesticide formulations compared to their active ingredients, as well as the potential for neurotoxicity, sensitization, oxidative stress, and the combined effects of pesticide mixtures. The findings underscore the importance of continuous investigation to protect human health in the face of evolving pesticide use and exposure. [Extracted from the article]

Off‐target drift of herbicides can seriously reduce peanut (Arachis hypogea L.) growth and yield and is of great concern to growers who will need to manage sensitive crops near new herbicide‐tolerant crops. Field experiments were conducted in 2021 and 2022 with 25% labeled rates of dicamba, glufosinate, glyphosate, lactofen, and paraquat to simulate drift on peanut. The objective was to evaluate the effects of low‐rate application of the herbicides on peanut injury and yield reductions and to determine if unmanned aerial vehicle (UAV) imagery‐based normalized difference vegetation index (NDVI) provides accurate estimation of peanut injury from the herbicides applied at vegetative (V3) and reproductive (R3) growth stages. Peanut suffered greater yield reduction (33%) when exposed to the herbicides at R3 than at V3 growth stage (19%) across all herbicides applied. The order of herbicides that induced yield reductions in peanut was glyphosate > glufosinate = dicamba > paraquat = lactofen. Regardless of exposure timing, NDVI values generated from UAV imagery could not differentiate paraquat or lactofen injury from the weed‐free check. However, NDVI values could differentiate between injured and weed‐free check plants up to 2 and 4 weeks after treatment (WAT) for dicamba at R3 and V3 exposure timing, respectively, up to 4 WAT for glufosinate, and 8 WAT for glyphosate. NDVI from aerial imagery may be helpful to accelerate the detection of injury in large hectarages with greater accuracy compared with visual injury rating, which can be influenced by individual estimation bias. Core Ideas: Peanut yield loss from 25% labeled rates of selected herbicides was greater at reproductive than at vegetative stage.Peanut was most susceptible to glyphosate (59%) and expressed the greatest tolerance to lactofen (≤10%).The order of yield reduction from the herbicides was glyphosate > glufosinate = dicamba > paraquat = lactofen. [ABSTRACT FROM AUTHOR]

Reported herein is an unprecedented protocol for C(sp3)‐phosphinylation. With 1 mol % 4CzIPN (1,2,3,5‐tetrakis(carbazol‐9‐yl)‐4,6‐dicyanobenzene) as the catalyst, the visible light induced reaction of redox‐active esters of aliphatic carboxylic acids with dimethyl arylphosphonites or diethyl alkylphosphonites at room temperature provides the corresponding decarboxylative phosphinylation products in satisfactory yields. The protocol exhibits broad substrate scope and wide functional‐group compatibility, enabling the late‐stage modification of complex molecules and rapid synthesis of bioactive phosphinic acids such as glutamine synthetase phosphinothricin and a kynureninase inhibitor. A radical‐polar crossover mechanism involving the formation and subsequent oxidation of phosphoranyl radicals followed by nucleophilic demethylation (or deethylation) is proposed. [ABSTRACT FROM AUTHOR]

Within the Ecuadorian native vegetation, weeds have been the plant that at any given time can compete for water, nutrients, light, space and carbon dioxide (CO2) with a crop, affecting productivity in quantity. and quality. Thus, this research aims to analyze the efficiency of two chemical herbicides for weed control in banana crops and thus determine the effect caused by both Ametrine and Ammonium Glufosinate. Based on the analysis of results, through the combined use of the herbicides Ametrine and Glufosinate ammonium, a higher percentage of effectiveness was observed in the control of weeds in the plantain crop (Musa × paradisiaca). Compared to the other treatments applied, this strategy is relevant since it guarantees good growth and essential development of the crop. [ABSTRACT FROM AUTHOR]

A spectral image analysis has the potential to replace traditional approaches for assessing plant responses to different types of stresses, including herbicides, through non-destructive and high-throughput screening (HTS). Therefore, this study was conducted to develop a rapid bioassay method using a multi-well plate and spectral image analysis for the diagnosis of herbicide activity and modes of action. Crabgrass (Digitaria ciliaris), as a model weed, was cultivated in multi-well plates and subsequently treated with six herbicides (paraquat, tiafenacil, penoxsulam, isoxaflutole, glufosinate, and glyphosate) with different modes of action when the crabgrass reached the 1-leaf stage, using only a quarter of the recommended dose. To detect the plant's response to herbicides, plant spectral images were acquired after herbicide treatment using RGB, infrared (IR) thermal, and chlorophyll fluorescence (CF) sensors and analyzed for diagnosing herbicide efficacy and modes of action. A principal component analysis (PCA), using all spectral data, successfully distinguished herbicides and clustered depending on their modes of action. The performed experiments showed that the multi-well plate assay combined with a spectral image analysis can be successfully applied for herbicide bioassays. In addition, the use of spectral image sensors, especially CF images, would facilitate HTS by enabling the rapid observation of herbicide responses at as early as 3 h after herbicide treatment. [ABSTRACT FROM AUTHOR]

A hollow fiber supported liquid membrane (HFSLM) was used to extract and eliminate glufosinate ammonium from aqueous solution using TOA (tri-n-octylamine) in hexane and sodium hydroxide (NaOH) as carrier and stripping phase, respectively. Maximum extraction (∼76%) and stripping (∼75%) were achieved by feed solution at pH 1.0, temperature 60 °C, TOA concentration 6% (v/v) and NaOH concentration 1.0 M. The developed mathematical model exhibited high precision when predicting the concentration of glufosinate ammonium in raffinate solution and extraction percentage under different feed flow rates, with correlation coefficient (R²) 0.9863. [ABSTRACT FROM AUTHOR]

Glufosinate is widely used to control various weeds. Glufosinate and its main metabolites have become the focus of attention because of their high water solubility and persistence in aquatic systems. Quantification of the agrochemical product and its metabolite residues is essential for the safety of agricultural products. In this study, a highly specific, simple method was developed to directly determine glufosinate and its metabolite residues in 21 plant origin foods by liquid chromatography with tandem mass spectrometry (LC–MS/MS), and it was validated on 11 foods in five laboratories. Finally, the repeatability limit, reproducibility limit, and uncertainty of the method were calculated based on these validated data and used to support the more accurate detection results. Four different chromatographic columns were used to analyze three target compounds, and the anionic polar pesticide column showed the optimum separation and peak shape. Composition of the mobile phase, extraction solvent, and the clean-up procedure were optimized. The developed method was validated on 21 plant origin foods. The average recoveries were 74–115% for all matrices. The validation results of five laboratories showed this method had a good repeatability (RSDr < 9.5%) and reproducibility (RSDR < 18.9%). The method validation parameters met the requirements of guidance established by the European Union (EU) and China for pesticide residue analysis. This methodology can be used for a routine monitoring that performs well for glufosinate and its metabolite residues. [ABSTRACT FROM AUTHOR]

Single-cell protein (SCP) is a promising alternative for replacing plant and animalderived dietary proteins. SCP contains essential nutrients and high levels of essential amino acids (AA). Given the versatility of microbial strains and waste substrates that can be used as feedstocks, many variations of production processes can be explored. Improving these microorganism strains by enhancing their properties and productivity is vital to increasing SCP competitiveness. One of the options to enhance microorganism strains would be by creating mutants with better AA profiles. By using mutagenesis and AA inhibitors it should be possible the create novel strains with improved AA-producing properties. The use of AA inhibitors to promote selective pressure on SCP-producing strains is a novel concept and is not a widely explored approach, therefore, the further development of this method should be explored. This paper used a multi-criteria decision analysis method to evaluate different technological factors vital for creating protein-rich mutants. These factors are microorganism strains, agro-industrial waste substrates used as process feedstocks, AA inhibitors, and mutagenesis methods. Microorganisms Candida utilis and Bacillus subtilis showed the highest potential for being used. Molasses was the 'closest to the ideal' substrate to be used as feedstock for SCP production. As the most promising mutagenesis method ethyl methane sulphonate was selected. Glufosinate ammonium and methionine sulfoximine for both bacteria and fungi were identified as the best inhibitors for SCP-rich mutant selection. Identified combinations of optimal solutions for microorganisms, substrates, inhibitors, and mutagenesis techniques should be further investigated and evaluated in laboratory settings. This could help to increase SCP's competitiveness as a sustainable protein source. [ABSTRACT FROM AUTHOR]

Over the past century, agriculture practices have transitioned from manual cultivation to the use of an array of chemical herbicides for weed control including phosphinothricin, or glufosinate (GLUF). Consequently, the potential for long-term residual GLUF exposure in the food chain has increased, highlighting the need for improved analytical strategies for its detection, as well as the detection of its main breakdown product 3-(methylphosphinico)-propionic acid (MPPA). Chemical derivatization strategies have been developed to improve the detection of GLUF and MPPA via liquid chromatography tandem mass spectrometry analyses. Herein, we employ trimethylation enhancement using diazomethane (TrEnDi) for the first time as a means to confer analytical advantages via quantitatively derivatizing these analytes into permethylated GLUF ([GLUFTr]+) and MPPA ([MPPATr+H]+). Comparing [GLUFTr]+ and [MPPATr+H]+ to underivatized counterparts, TrEnDi yields 2.8-fold and 1.7-fold improvements in reversed-phase chromatographic retention, respectively, while MS-based sensitivity is enhanced 4.1-fold and 11.0-fold, respectively. Successful analyte derivatization (with >99% yields) was further demonstrated on a commercial herbicide solution imparting consistent analytical enhancements. To investigate the benefits of TrEnDi in a bona fide agricultural scenario, simple aqueous extractions from distinct parts of field-grown canola plants were performed to quantify GLUF and MPPA before and after TrEnDi derivatization. In their underivatized forms, GLUF and MPPA were undetectable in all field samples, whereas [GLUFTr]+ and [MPPATr+H]+ were readily quantifiable using the same analysis conditions. Our results demonstrate that TrEnDi continues to be a useful tool to enhance the analytical characteristics of organic molecules that are traditionally difficult to detect. [ABSTRACT FROM AUTHOR]

Copyright of Revista U.D.C.A Actualidad & Divulgación Científica is the property of Universidad de Ciencias Aplicadas y Ambientales U.D.C.A and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purpose: The analysis of water-soluble herbicides, including glyphosate (Glyp), glufosinate (Gluf), paraquat (PQ), and diquat (DQ), is time-consuming and expensive because they cannot be analyzed using general toxicological screening methods. Thus, this study aimed to develop a simple and rapid method to simultaneously analyze these compounds without any derivatization nor ion-pairing reagents. Methods: The analytes were separated using hydrophilic interaction liquid chromatography and detected using tandem mass spectrometry. The developed method was applied to plant and biological samples assuming criminal damage and poisoning cases, respectively. Results: All analytes were separated well and detected with good peak shapes. For plant samples, the herbicides were specifically detected from withered leaves using a simple extraction method. For biological samples, quantitative analysis was successfully validated, and the limit of quantification values of Glyp and Gluf were 0.2 µg/mL, and those of PQ and DQ were 1 ng/mL. Conclusion: The developed method had sufficient performance for practical forensic applications including poisoning cases and malicious uses to damage commercial crops. [ABSTRACT FROM AUTHOR]

Abstract A putative glufosinate‐resistant Amaranthus palmeri population was reported in 2015 in Anson County, North Carolina. The results from dose–response assays conducted in the field suggested plants were surviving lethal rates of glufosinate. Dose–response assays conducted in the glasshouse determined the Anson County accession exhibited reduced susceptibility to glufosinate compared to three glufosinate‐susceptible populations. The LD50 values (210–316 g ai ha−1) for the Anson County population were always higher than the LD50 values (118–158 g ai ha−1) for the tested susceptible populations from the dose–response assays. Anson County plants that survived lethal glufosinate rates were reciprocally crossed with susceptible plants to create F1 genotypes and treated with a lethal rate of glufosinate (267 g ai ha−1; ascertained from glasshouse dose–response assay) to determine the distribution of injury and survival for each cross compared to a cross of susceptible parents. The distribution of injury was non‐normal for the crosses containing an Anson County plant compared to the cross with a susceptible parent. Survival was 68%–84% for crosses containing an Anson County plant, whereas the survival was significantly reduced to 35% for the susceptible plant cross. Chi‐square goodness of fit tests were used to test inheritance models to describe the responses of the genotypes. The resistant × susceptible crosses were best described with a heterozygous two loci with incomplete dominance model compared to the resistant × resistant cross that was best described with a heterozygous single locus with incomplete dominance model. The Anson County population has evolved resistance to glufosinate that is heritable and likely conferred by an oligogenic mechanism with incomplete dominance.

Glyphosate and glufosinate are among the most widely used pesticides in agriculture worldwide. Their extensive use leads to the presence of their residues on crops and in the surrounding environment. Beehives, bees, and apiculture products can represent potential sources for the accumulation of these substances and their metabolites, and the consequences for bee health, as well as the level of risk to human health from consuming contaminated food, are still unclear. Furthermore, information on the contamination levels of honey and other beehive products by these compounds remains poorly documented. This study is part of a broader research effort aimed at developing specific analytical methods for monitoring the level of these contaminants in bee products. The methodology employed enabled the acquisition of preliminary information concerning the levels of glyphosate and glufosinate contamination in honey samples obtained from various retailers in Italy to assess compliance with the limits established by Regulation 293/2013. The liquid chromatography tandem mass spectrometry analysis of the 30 honey samples revealed quantifiable levels of glyphosate in eight samples, with contamination ranging from 5.4 to 138.5 ng/g. Notably, one sample of the wildflower type showed residue levels nearly three times the maximum residue limit. Additionally, trace levels of glyphosate contamination were detected in another ten samples. It is noteworthy that glufosinate and its metabolites were not detected in any of the analyzed samples within the established method’s detection ranges.

Environmental and genetic factors influence synapse formation. Numerous animal experiments have revealed that pesticides, including herbicides, can disturb normal intracellular signals, gene expression, and individual animal behaviors. However, the mechanism underlying the adverse outcomes of pesticide exposure remains elusive. Herein, we investigated the effect of maternal exposure to the herbicide glufosinate ammonium (GLA) on offspring neuronal synapse formation in vitro. Cultured cerebral cortical neurons prepared from mouse embryos with maternal GLA exposure demonstrated impaired synapse formation induced by synaptic organizer neuroligin 1 (NLGN1)-coated beads. Conversely, the direct administration of GLA to the neuronal cultures exhibited negligible effect on the NLGN1-induced synapse formation. The comparison of the transcriptomes of cultured neurons from embryos treated with maternal GLA or vehicle and a subsequent bioinformatics analysis of differentially expressed genes (DEGs) identified "nervous system development," including "synapse," as the top-ranking process for downregulated DEGs in the GLA group. In addition, we detected lower densities of parvalbumin (Pvalb)-positive neurons at the postnatal developmental stage in the medial prefrontal cortex (mPFC) of offspring born to GLA-exposed dams. These results suggest that maternal GLA exposure induces synapse pathology, with alterations in the expression of genes that regulate synaptic development via an indirect pathway distinct from the effect of direct GLA action on neurons. [ABSTRACT FROM AUTHOR]

The prevalence of Huanglongbing (HLB) disease, also known as citrus greening, has compelled the citrus industry to change management practices to increase production. However, these changes, such as enhanced nutrition and irrigation programs, have caused weed proliferation, subsequently leading to increased use of herbicides. Thus, our study evaluated a popular preemergence herbicide active ingredient, diuron, for nontarget impacts on young Hamlin and Valencia sweet orange (Citrus sinensis L. Osbek) trees in two commercial orchards in southwest Florida. The treatments included the preemergence application of diuron at three rates (1.8, 3.6, and 7.3 kg a.i./ha), a weed-checked control (using postemergence herbicides: glufosinate ammonium + saflufenacil), and a nontreated control. The treatments were applied twice (in Fall 2021 and Spring 2022) in a randomized complete block with four replicates. Results indicate that over a 5-month period, the application of diuron generally had no significant impact on citrus root growth. Further, over a 2-month observation period for Hamlin and a 4-month observation period for Valencia, it was found that diuron application had no notable effect on fruit detachment force. Valencia trees treated with diuron high showed higher HLB disease severity at location 1. In addition, Hamlin trees treated with diuron low and medium showed higher fruit drop (~19% more) than the untreated control at location 1. However, this trend was inconsistent across the locations and cultivars. This result suggests that increased disease severity and fruit drop were not associated with diuron treatment. Thus, our study finds diuron as a tree-safe option for preemergence weed suppression in citrus production, as long as it is used in accordance with the recommended dosage and restrictions stated on the herbicide label. [ABSTRACT FROM AUTHOR]

Multiple herbicide-resistant waterhemp (Amaranthus tuberculatus (Moq.) J.D. Sauer) has limited herbicide options for Iowa row crop production. Soybean (Glycine max (L.) Merr.) cultivars and corn (Zea mays L.) hybrids resistant to 2,4-D, dicamba, and glufosinate have provided more herbicide options for waterhemp management. A probability-based survey of Iowa fields was conducted in fall 2019 wherein waterhemp seeds were collected and their susceptibility to 2,4-D, dicamba, and glufosinate was determined. The average survival of all populations treated with 1× 2,4-D, dicamba, and glufosinate was 17%, 5%, and 4%, respectively. Seven percent of treated populations exhibited ≥50% survival to 1× rates of 2,4-D. [ABSTRACT FROM AUTHOR]

Despite potential health implications, data on the presence of Glyphosate (GLY) and other non-GLY herbicides in human matrices remain scarce. This study aimed to develop a simple and cost-effective methodology for detecting and quantifying GLY, its primary biodegradation product; aminomethylphosphonic acid (AMPA); and glufosinate (GLU) in plasma and urine of environmentally and occupationally exposed populations from the province of Córdoba (Argentina). Different alternatives of pre-treatment, derivatization with FMOC-Cl, solid phase extraction, and final sample conditioning steps were evaluated to improve the quantification of the herbicides by a high-performance liquid chromatography system coupled to a triple-quadrupole mass spectrometer. Recoveries ranged from 39 to 84% in both matrices, while limits of quantification were 3, 1, and 0.3 ng/mL and 3.6, 5.1, and 0.3 ng/mL for AMPA, GLY, and GLU in plasma and urine, respectively. In plasma samples, GLY was the most frequently detected analyte (32%), followed by GLU (10%). In urine samples, GLU was the most frequently detected herbicide (13%), followed by GLY (6%). No differences between group or matrix correlations were found. This study is the first report of GLU in human biological matrices and should be used to establish baseline values for future surveillance systems. [ABSTRACT FROM AUTHOR]

The skin sensitization potential of agrochemicals can be assessed using laboratory methods such as the keratinocyte activation assay so that their use in regulatory toxicology might replace experimental animal testing. Here, we evaluated the skin sensitization potential of 11 agrochemicals by using an antioxidant response element–nuclear factor erythroid 2 luciferase assay in KeratinoSens and LuSens cells and applying a skin sensitization adverse outcome pathway (AOP). The KeratinoSens and LuSens assays consistently evaluated the skin sensitization potential of 10/11 agrochemicals with reference to animal testing databases. Benomyl, pretilachlor, fluazinam, terbufos, butachlor, and carbosulfan were correctly detected as sensitizers, and glufosinate ammonium, oxiadiazon, tebuconazole, and etofenprox were correctly detected as non-sensitizers. For diazinon, the skin sensitizing potential was positive in the KeratinoSens assay but not in the LuSens assay. These results suggest that the evaluation of in vitro skin sensitization using the AOP mechanism can be applied to assess active agrochemicals. [ABSTRACT FROM AUTHOR]

Copyright of Agronomy Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The present work reports on the design, execution and evaluation of results of an interlaboratory validation study aimed at verifying the fitness-for-purpose of a LC-MS/MS method for the detection of polar pesticides in food of animal origin in official control and monitoring programmes. To this scope, five participant laboratories, with relevant expertise, were recruited. After passing a pre-trial test, the participants were asked to analyse test samples of bovine fat, chicken eggs and cow's milk, contaminated with 11 polar pesticides (group A: Aminomethyl phosphonic acid (AMPA), cyanuric acid, ethephon, glyphosate, fosetyl aluminium, 2-hydroxyethyphosphonic acid (HEPA), maleic hydrazide, N-acetyl-glyphosate, group B: N-acetyl glufosinate (NAG), 3-methylphosphinicopropionic acid (MPP) and glufosinate ammonium) at two different levels (0.05 and 0.25 mg/kg−1 and 0.01 and 0.05 mg/kg−1 for group A and B respectively. The method was based on acidified methanol/water extraction followed by dSPE clean up with C18 sorbent. For LC-MS/MS analysis isotopically labelled standards were used for all targeted analytes. With a couple of exceptions, average recoveries ranged from 85% to 110%, with repeatability (RSDr) ranging from 3% to 25%, and reproducibility (RSDR) from 4% to 26%. The assessment by different laboratories provided also insights on key factors impacting method performance characteristics and its implementation by new users. [ABSTRACT FROM AUTHOR]