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Brucellosis is a highly contagious zoonotic bacterial disease. It has considerable negative consequences on the animal production industry worldwide. The objective of this study was to investigate the genetic and molecular variations in Shami goat susceptible to Brucella infection. Blood samples were collected from fifty mature Shami goats (30 Brucella-infected does and 20 non-infection). DNA was extracted and selected parts the immunity; solute carrier family 11 member 1 (SLC11A1), toll-like receptor 1 (TLR1), toll-like receptor 9 (TLR9), SP110 nuclear body protein (SP110), the adenosine A3 receptor (ADORA3), caspase activating recruitment domain 15 (CARD15) and interferon regulatory factor 3 (IRF3), antioxidant glutathione peroxidase 1 (GPX1), nitric oxide synthase (NOS), NAD(P)H dehydrogenase [quinone] 1 (NQO1) and transcription factor NF-E2-related factor 2 (Nrf2) and erythritol related transketolase (TKT), ribose 5-phosphate isomerase (RPIA) and Adenosine monophosphate deaminase (AMPD) genes were sequenced. Likewise, the levels of gene expressions were investigated. The results identified polymorphic variants between healthy and infected does. Levels of gene expression of SLC11A1, TLR1, TLR9, SP110, ADORA3, CARD15, IRF3, HMOX1, TKT, RPIA and AMPD were significantly (P < 0.05) up regulated in the infected compared to the non-infected ones. On the other hand, GPX1, NOS, NQO1 and Nrf2 genes were significantly (P < 0.05) downregulated in the infected compared to the non-infected does. The results of serum profile indicated that there is a significant (P < 0.05) increase in the activities of AST, ALT, GGT, LDH, ALP as well as serum level of globulin, triglycerides, cholesterol, MDA, NO, IL-1β, TNF-α, IgM, IgG, haptoglobin and amyloid A. On the other hand, there were significant reductions in the glucose, total protein albumin, urea, calcium, inorganic phosphorus, sodium, copper, zinc, iron, TAC, GSH, SOD, GPx, IL-10 and fibrinogen in the infected compared to the non-infected does. Our results provide valuable information about the serum profile variations and putative genetic markers for Brucella infection in goats. This could be utilized in controlling goat brucellosis through selective breeding of natural resistant animals. [ABSTRACT FROM AUTHOR]

BACKGROUND: Depression is a common psychological disorder, and traditional therapeutic drugs often result in side effects such as emesis, dry mouth, headache, dysentery and constipation. Probiotics and goat milk have garnered widespread attention for their ability to modulate immune function and regulate the endocrine system, and for their anti‐inflammatory effects. In this work, the effects of Tibetan goat kefir on the behavior, immune status, neuroendocrine response and gut microbiological composition of chronic unpredictable mild stress (CUMS) mouse models were evaluated. RESULTS: The results indicated that Tibetan kefir goat milk significantly alleviated behavioral despair in mice. Furthermore, the results demonstrated that Tibetan kefir goat milk mitigated the inflammatory response in the mice and moderated the hyperactivity of the hypothalamic–pituitary–adrenal axis and the expression of brain‐derived neurotrophic factor. Meanwhile, chronic stress‐induced gut microbial abnormalities were restored. In addition, the correlation between gut microbiota and nervous system was evaluated. CONCLUSION: These results explained the potential mechanism of Tibetan kefir in the antidepressant effect on the CUMS model and enriched diets for depressed patients. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. This study investigated the effect of high-pressure processing (HPP) on the physicochemical and structural changes in reconstituted micellar CN concentrates (MCC) sourced from bovine (BMCC) and caprine (CMCC) milk. When applying pressures ranging from 100 to 500 MPa for 30 min at 25 ± 2°C, it was observed that BMCC exhibited a notable increase in soluble minerals and solubility percentages with a simultaneous reduction in particle size and zeta potential compared with CMCC. In BMCC, Ca and P solubility rose from 27.71% and 33.02% to 90.33% and 92.01%, respectively. In CMCC, the solubility of Ca and P increased from 36.12% and 38.29% at 100 MPa to 52.57% and 56.77% at 500 MPa. Additionally, the reduction in particle size was more pronounced in BMCC, with a 53% decrease at 500 MPa, compared with a 19.90% reduction in CMCC. Furthermore, fluorescence intensity, indicative of surface hydrophobicity, decreased in BMCC samples (from 3,478 to 2,571 arbitrary units [a.u.]), whereas it increased in CMCC samples (from 2,388 to 4,058 a.u.) across the same pressure range. Comparative analysis revealed that HPP-treated CMCC samples exhibited a decline in α-helix content until the treatment at 300 MPa, followed by an increase post-treatment at 500 MPa. Additionally, a rise in β-sheet structure content was observed, except for the sample treated at 500 MPa, where β-sheet content significantly decreased. These findings suggest that HPP holds promise as a viable technological approach in the dairy sector for enhancing the functional characteristics of CMCC. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. This study developed an efficient method for identifying and quantitatively analyzing animal-origin milk powders using Raman spectroscopy combined with chemometrics. By employing the MultiClassClassifier model, the method achieved high accuracy in distinguishing various types of animal-origin milk powders, with sensitivity and specificity both exceeding 80% and an overall accuracy of 93%. Furthermore, the quantitative models based on partial least squares regression and support vector machine regression exhibited excellent linear correlations, with both root mean square error and mean relative error below 0.2. These models successfully quantified adulteration in camel, mare, and donkey milk powders in comparison to goat and cow milk powders. The study's approach not only holds significant promise for detecting adulteration in specialty milk powders but also demonstrates wide applicability in analyzing other powdered adulterants. [ABSTRACT FROM AUTHOR]

Copyright of Canadian Journal of Veterinary Research / Revue Canadienne de Recherche Vétérinaire is the property of Canadian Veterinary Medical Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Cryptosporidium spp. are protozoan parasites that infect the gastrointestinal tract of various animals, including goats, and can also contaminate water sources, posing a significant public health risk. Detecting Cryptosporidium oocysts in fecal and water samples is critical for understanding the epidemiology of cryptosporidiosis and implementing appropriate control measures. Various staining methods, such as the Modified Ziehl-Neelsen (ZN) and Kenyon's Acid-Fast (KAF) staining techniques, are employed to identify these oocysts. This study compared the effectiveness of these two staining methods in detecting Cryptosporidium oocysts in goat feces and water samples across different geographic regions in district of Mardan, Khyber Pakhtunkhwa, Pakistan, and other factors such as genders, age groups, diarrheal statuses, and feeding regimes. Methods: A total of 300 fecal and 300 water samples were collected from goats and water sources in four geographic regions: Tehsil Katlang, Tehsil Takhtbhai, Tehsil Mardan, and Tehsil Lundkhwarh. Samples were categorized based on gender, age group (<1 year, 1–2 years, and >2 years), diarrheal status, and feeding regime (stall feeding, grazing). The two staining methods, ZN and KAF staining, were employed to detect Cryptosporidium oocysts. The detection rates were calculated, and statistical analyses were performed to compare the effectiveness of the two methods across different categories. Results: The overall detection rates of Cryptosporidium oocysts for fecal samples were 61.00% (95% CI: 55.22–66.55%) using the ZN method and 63.33% (95% CI: 57.60–68.79%) using KAF method, with no significant difference (p > 0.05). The highest detection rate was observed in Tehsil Katlang (70.66%) with the ZN method and in both Tehsil Katlang and Takhtbhai (66.66%) with the KAF staining method, having no significant difference (p > 0.05). Gender-wise analysis in fecal samples showed similar detection rates for males and females, with no significant differences. Age-wise, the highest detection rates were found in the youngest age group (<1 year) using the ZN method, and in the oldest age group (>2 years) using KAF staining, with no significant differences between age groups. Diarrheal status analysis indicated higher detection rates in diarrheic goats for both methods, with the highest detection rate in the diarrheic group of Tehsil Katlang (84.61%) using the ZN method. Feeding regime analysis showed no significant differences between stall-fed and grazing goats. For water samples, the detection rates of Cryptosporidium oocysts were significantly different between the two methods. The ZN technique had a significantly higher overall detection rate of 16.00% (95% CI: 12.03–20.64%) compared to 1.00% (95% CI: 0.20–2.89%) for KAF staining (p < 0.001). The performance of the two staining methods for the detection of Cryptosporidium oocysts in contaminated water samples from different geographic regions was also presented. Conclusion: Both ZN and KAF staining methods are effective for detecting Cryptosporidium oocysts in goat feces. However, in water samples, the ZN method showed a significantly higher detection rate compared to KAF staining method, suggesting its suitability for environmental surveillance. These findings highlight the importance of integrating reliable diagnostic techniques with public health interventions to mitigate the zoonotic risks of cryptosporidiosis. [ABSTRACT FROM AUTHOR]

Relatively high milk-producing cows in Morocco are culled at an elevated rate due to poor fertility. This situation is due to genetic, environmental, and management-related factors. To remedy this situation or prevent an additional decline in reproductive performance, using locally produced replacement heifers with good fertility and yield traits was proposed as a solution. Identifying these animals requires estimating the genetic parameters of the relevant reproduction and production traits in order to develop a genetic evaluation. Three reproduction traits (number of inseminations per conception, success of first insemination, and days open) and 305-d milk yield were used in this study. Two datasets of 4,186 records (first parity cows) and 5,511 records (first and multi-parity cows) were used. The pedigree files for both datasets consisted of 8,758 and 9,935 animals, respectively. A threshold-linear model was used for the analyses. For the first parity, estimates of heritability for 305-day milk yield (MY), days open (DO), number of inseminations per conception (NIC), and success of first insemination (SFI) were 0.26 ± 0.04, 0.17 ± 0.04, 0.10 ± 0.03, and 0.10 ± 0.04, respectively. For multi-parity data, the estimates were 0.19 ± 0.03, 0.12 ± 0.02, 0.10 ± 0.02, and 0.09 ± 0.02 for MY, DO, NIC, and SFI, respectively. The genetic correlations between MY and reproduction traits were 0.15 ± 0.11, 0.38 ± 0.12, and -0.43 ± 0.11 for DO, NIC, and SFI respectively. Overall, the heritability estimates of fertility traits were low. The genetic correlations of DO, NIC, and SFI with MY were moderately negative allowing for further selection for milk production without an additional deterioration in reproductive performance. The relative impact of using high fertility bulls compared to low fertility bulls on the success of first insemination ranged between 1.2% and 6.3% depending on the production environment. Collectively, these results confirm the possibility of implementing a viable selection program based on an appropriate weighted selection index. [ABSTRACT FROM AUTHOR]

Fermented dairy products have been studied for their physiological functions, particularly their antioxidant properties. Dadih is a traditional Indonesian fermented dairy product produced from buffalo milk and prepared in bamboo tubes that exhibit functional properties. Compared to cow milk, goat milk has gained more attention for dadih production due to its low allergen count and ease of digestion. In our previous study, we identified the dipeptide glycine‐methionine (Gly‐Met) generated from goat milk dadih as a novel antioxidant peptide. This study aimed to investigate the antiaging effects of Gly‐Met in Caenorhabditis elegans using an in vivo model. Initially, the effective concentration (EC50) was determined and Gly‐Met exhibited an effective antioxidant activity value of 0.96 mg/mL. Our results demonstrated that Gly‐Met significantly extended the mean lifespan, enhanced resistance to oxidative stress, and reduced reactive oxygen species (ROS) accumulation in C. elegans. Furthermore, the mRNA expression of Nsy-1, Sek-1, and Pmk-1 that are the target genes of the p38 MAPK signaling pathway was upregulated in C. elegans. Subsequently, Skn-1 and Gcs-1 expression levels were significantly upregulated. Activation of SKN‐1 target genes may be modulated by the p38 MAPK signaling pathway. Our study revealed that Gly‐Met, an antioxidative peptide identified in goat milk dadih, may contribute to antiaging effects through the SKN‐1/Nrf2 signaling pathway in C. elegans. [ABSTRACT FROM AUTHOR]

Potentially toxic elements (PTEs) were determined in raw milk from Nigerian cattles (cow, goat and sheep), to assess the environmental pollution, impact on milk quality and health risk. Nine composite samples were collected from five states in Northwest Nigeria, being the major cattle breeders. The samples were wet digested and PTEs determined using ICP-OES. 45 PTEs were ascertained of which Nitrogen (43754 mg/L) and Strontium, Sr (0.007 mg/L) recorded the highest and least mean concentration respectively. The mean concentration of other PTEs were: Selenium (0.253 ± 0.077), Cadmium (0.040 ± 0.0411), Mercury (0.048 ± 0.037), Silver (0.088 ± 0.013), Arsenic (0.044 ± 0.006), Boron (0.056 ± 0.005), Aluminium (0.014 ± 0.002), Tin (0.109 ± 0.019), Lead (0.018 ± 0.006), Gold (0.088 ± 0.048), Manganese (214.8 ± 18.754), Iron (473.9 ± 55.901), Zinc (247.9 ± 15.874), Copper (84.5 ± 12.905), Nickel (0.0031 ± 0.001), Molybdenum (0.459 ± 0.160), Cobalt (0.055 ± 0.020), Chromium (0.028 ± 0.008), Vanadium (0.185 ± 0.051), Calcium (24988 ± 16302), Magnesium (5733 ± 1460.8), Potassium (6555 ± 1199.6), Sodium (17.59 ± 2.914), Phosphorus (497.2 ± 90.38), Bismuth (0.055 ± 0.032), Cerium (0.048 ± 0.043), Argon (0.083 ± 0.011), Silicon (0.265 ± 0.110), Sulphur (0.087 ± 0.009), Titanium (0.073 ± 0.030) and Bromine (0.021 ± 0.005). Others are Platinum (0.021 ± 0.008), Antimony (0.088 ± 0.007), Iodine (0.16 ± 0.022), Caesium (0.374 ± 0.037), Barium (0.025 ± 0.009), Lanthanum (0.115 ± 0.182), Samarium (0.150 ± 0.095), Palladium (1.47 ± 0.991), Curium (0.042 ± 0.009), Fermium (0.036 ± 0.0098), Americium (0.826 ± 0.618) and Rubidium (0.048 ± 0.044). The Pearson's correlation and paired sample t-test showed that most of the PTEs have similar origin at 0.05% significant level, and all the cattle had the same level of exposure to the PTEs respectively in the area. Raw milk is about 80% water and so, the determined PTEs concentrations are considered very high in the analyzed samples. The presence of Pb in the milk is a concern since Pb is a toxin at any concentration. The high content of PTEs in milk is attributed to the indiscriminate and artisanal mining activities in the region, and the fact that the animals are free rangers. The health risk factors were below unity (HR < 1) and so were not potential hazards. However, the environmental contaminations by these PTEs are very substantial and if this type of milk is taken raw or used for the production of dairy products like cheese and butter, then there may be a serious health condition on the consumer since they are not biodegradable. [ABSTRACT FROM AUTHOR]

Simple Summary: In arid and semi-arid regions of the world, goats are typically raised in rangeland conditions at latitudes above 30° N, dealing with significant annual fluctuations in the rainfall pattern, dictating, in turn, the quantity and quality of food availability, particularly in marginal goat production systems (GPSs). Under such a scenario, providing commercial grains and concentrates can be costly, making it difficult for most goat farmers to afford. Hence, the effective use of the rangeland biotic resources aligned with the amount of rainfall is key in determining the productivity of any production system, especially those with a dairy emphasis. We identified a compelling research opportunity focused on elucidating the key features that distinguish various dairy GPSs (i.e., DGPSs). Interestingly, DGPSs can be approached from the most traditional extensive system, resembling a more circular, less linear, more closed loop, mainly based on a circular economy approach and generally observed in developing economies, moving up to those less classical, highly industrialized, large-scale, linear-based dairy goat systems, mainly observed in developed economies. This review aims to emphasize the important role that goats and dairy goats play for many small-scale rural families worldwide, as well as to introduce a proposal for categorizing the main dairy goat production systems (DGPSs), using a multifactorial approach but emphasizing rainfall and nutritional supplementation level, as the focal categorization factors. The main DGPSs were divided into two metasystems based on available resources, each consisting of three production subsystems. In the first metasystem, the three subsystems have limited water, biotic, and economic resources, whose main economic rationality is based on reducing risk rather than maximizing outputs. In contrast, the three subsystems of the second metasystem usually have increased biotic, economic, and water resources, whose main emphasis involves maximizing product yield rather than reducing risk. The first metasystem involves DGPSs with a very limited or null nutritional supplementation: (a) subsistence, (b) extensive, and (c) agro-silvopastoral. The second metasystem includes those DGPSs with different levels of nutritional supplementation: (d) semi-extensive, (e) semi-intensive, and (f) intensive. There are numerous significant global initiatives focused on scientific collaboration and sharing information regarding nutrition, reproductive, and genetic technologies related to the safety and nutraceutical quality of goat milk and products while contextualized in different DGPSs. Hence, such scenarios should create additional opportunities for researchers, producers, policymakers, and development workers to come together and align interests and needs and exchange knowledge on effective goat farmer support strategies, environmental management, and consumer education. Undoubtedly, it is essential to reevaluate the DGPSs in the world since millions of producers and their families—most of them poor and marginalized—need this species, society needs their products, most of the worldwide arid and semi-arid lands need their recovery, and all of us should encourage the fulfillment of the sustainable development goals. [ABSTRACT FROM AUTHOR]

Objectives: Small extracellular vesicles (sEVs) are nanosized vesicles with biological activities that exist in milk, playing functional roles in immunity, gut balance, and the nervous system. Currently, little is known about the impact of processing on milk sEVs. Methods: In this study, sEVs were collected from raw goat milk (g-sEV), pasteurized goat milk (pg-sEV), and goat milk powder (p-sEV) using a sucrose cushion centrifugation combined with qEV chromatography. Then, the sEVs were identified and compared using NTA, Western blot, and TEM. After extracting RNA and the total proteome from sEVs derived from different samples, the RNA was subjected to high-throughput sequencing, and peptide fragments were analyzed using mass spectrometry. Finally, GO and KEGG pathway analyses were performed on the results. Results: The characterization results revealed a decrease in diameter as the level of processing increased. High-throughput sequencing results showed that all three types of small extracellular vesicles were found to be rich in miRNA, and no significant differences were observed in the most abundant sEV species. Comparing with g-sEV, there were 3938 and 4645 differentially expressed miRNAs in pg-sEV and p-sEV, respectively, with the majority of them (3837 and 3635) being downregulated. These differentially expressed miRNAs were found to affect biological processes or signaling pathways such as neurodevelopment, embryonic development, and transcription. Proteomic analysis showed that there were 339 differentially expressed proteins between g-sEV and pg-sEV, with 209 proteins being downregulated. Additionally, there were 425 differentially expressed proteins between g-sEV and p-sEV, with 293 proteins being downregulated. However, no significant differences were observed in the most abundant protein species among the three types of sEVs. Enrichment analysis indicated that the differentially expressed proteins were associated with inflammation, immunity, and other related processes. Conclusions: These results indicate that extracellular vesicles have a protective effect on their cargo, while processing steps can have an impact on the size and quantity of the sEVs. Furthermore, processing can also lead to the loss of immune-related miRNA and proteins in sEVs. [ABSTRACT FROM AUTHOR]

The article "Food-Induced Anaphylaxis Reactions at School: A Room for Improvement" discusses the prevalence and management of food-induced anaphylaxis in school-aged children. The study analyzed data from the Allergy-Vigilance Network database from 2002 to 2023, revealing that 8.9% of 3119 recorded food-induced anaphylaxis reactions occurred at school. The main food triggers were peanut and cow, goat, and sheep milk, with varying severity levels and treatment outcomes. The study highlights the need for improved prevention and management strategies, including individual healthcare plans and increased awareness and training for school staff. [Extracted from the article]

The aim of this study was to determine the effects of supplementing extruded and pelleted starter feeds on growth performance, rumen development, carcass characteristics, and sensorial meat quality of goat kids fed an all-milk protein replacer. Forty-five new-born French Alpine kids (21 males, 4.93 ± 0.58 kg; and 24 females, 4.40 ± 0.60 kg) were randomly assigned to three treatment diets, milk replacer-only (RO), milk replacer supplemented with extruded starter feed (RE), or milk replacer supplemented with pelleted starter feed (RP) in a 3 x 2 factorial arrangement. After 35 days, the final weights, weight gain, body size measurements, organ weights, and hot and cold carcass weights were greater (p < 0.05) for goat kids in the RO group compared to those in the RE and RP groups. Milk replacer intake was reduced (p = 0.000) with starter supplementation, and intake of the extruded starter was higher (p = 0.001) than that of the pelleted starter. The rumen weight was greater (p = 0.019) in the RE group than in the RO group. Rumen pH was higher (p = 0.006) and acetic acid (p = 0.008) and total VFA (p = 0.012) concentrations were lower in the RO group than in the RE and RP groups. Mesenteric fat content was higher (p = 0.002) in the RO group than in the other groups. Meat odor of the RO group was preferred (p = 0.051) by the sensorial panel. Feeding an RO diet is beneficial for goat kids sold to restaurants. For replacement kids, an extruded starter feed may improve rumen development and facilitate adaptation to post-weaning dry feed consumption. [ABSTRACT FROM AUTHOR]

Doxycycline is an antimicrobial agent used in veterinary medicine to treat a variety of bacterial infections. To date, no analytical technique utilising HPLC with fluorescence detection has been documented for the quantification of doxycycline concentrations in goat plasma or milk. Consequently, the objective of the present study was to propose a rapid HPLC assay with fluorescence detection for the quantification of doxycycline in the aforementioned samples, thereby facilitating the conduct of pharmacokinetic studies and the detection of residues in diverse goat tissues. Proteins were precipitated with methanol and trifluoroacetic acid in a single step. Doxycycline was separated on a XBRIDGE C18 column using an isocratic method. Sample volume injected into the HPLC system was 50 µl. Fluorescence detection was conducted with an excitation wavelength of 380 nm and an emission wavelength of 520 nm. The retention times of doxycycline and danofloxacin (internal standard) were determined to be 8.0 and 5.5 minutes, respectively. The calibration curves for plasma and milk exhibited linearity over the concentration range of 0.1 to 2 μg/mL. The limit of detection was 0.065 μg/ mL, while the limit of quantification was 0.1 μg/mL in both matrices. The accuracy and precision of the method were consistently within the limits of 10.9% for plasma and 10.5% for milk. The findings of this study may be employed in the quantification of doxycycline in goat plasma and milk, thus facilitating the conduct of pharmacokinetic studies. [ABSTRACT FROM AUTHOR]

The purpose of this study was to examine at the chemical content of Hermosa sidoides and Grewia tembensis feeds and their impact on milk output and quality in Somali goats. A completely randomized block design, excluding the control treatment, was adopted with three treatment diets. T1 (600 g Hermosa sidoides, 200 g concentrate mixture, maize stover), T2 (600 g Grewia tembensis, 200 g concentrate mixture, maize stover), and T3 (300 g Hermosa sidoides and 300 g Grewia tembensis, maize stover), were the treatments. Hermosa sidoides had a greater crude protein (CP) content (28.7%), 40% neutral detergent fibre (NDF), and 18% acid detergent fibre (ADF), while Grewia tembensis had a lower CP content (27.1%), 47% NDF, and 21.91% ADF. The daily milk production for goats fed treatment food T1 (0.91 kg) was substantially greater (p0.05) than goats fed T2 (0.82 kg) and T3 (0.84 kg) after 90 days of feeding experiments. In general, this study found that supplementing nursing Somali goats' diets with Hermosa sidoides (Qaxxee) leaves (T1 Diet) considerably increases milk output and cost return. As a result, this study implies that goat feeds, which are often available in the study location, could be used as a suitable diet in the future. [ABSTRACT FROM AUTHOR]

Hexachlorobenzene (HCB) is a persistent organic pollutant (POP) commonly detected in milk and dairy products. These compounds pose a serious threat to the health of consumers due to their considerable bioaccumulation potential, high stability, and toxicity. (2) Methods: The study evaluated the potential of Lactiplantibacillus plantarum and Lacticaseibacillus rhamnosus probiotic cultures to reduce HCB residues in fermented goat milk beverages during 21-day refrigerated storage. HCB content was determined by gas chromatography coupled with mass spectrometry (GC/MS). (3) Results: A strong negative correlation was found between HCB concentration in fermented milk and storage time. After 21 days, a 75–78% reduction in HCB content was observed, with L. plantarum showing greater efficiency in reducing hexachlorobenzene levels than L. rhamnosus. (4) Conclusions: The use of probiotic cultures contributed to a significant reduction in the HCB content of fermented goat milk. Our findings support the hypothesis that the lactic acid bacteria Lactiplantibacillus plantarum and Lacticaseibacillus rhamnosus can lower hexachlorobenzene levels in fermented products [ABSTRACT FROM AUTHOR]

Simple Summary: At the beginning of the lactation period, primiparous goats are likely to face stressful situations during milking procedures that may negatively affect milk yield and quality. The milking reactivity of 31 primiparous Saanen goats was assessed on days 10, 30, and 60 of the lactation period by scoring step-kick behavior (SK) and head, ear, and tail movements (MOV) during the milking procedure. Milk yield, milk protein and fat percentages, somatic cell count, and plasma cortisol concentrations were measured on day 10 of lactation. Goats' milking reactivity was classified as low or high, according to the SK and MOV scores measured on day 10 of the lactation period. There was a decrease in the SK and MOV scores over the lactation period. Milking reactivity affected only milk fat percentage, with the milk of goats with low reactivity having more milk fat than those with high reactivity. There was no evidence of stress when goats were subjected to milking procedures early in lactation. Further studies are needed to clarify the relationships of milking reactivity with stress response and their effects on milk yield and quality. The reactivity of primiparous goats during milking can significantly affect animal welfare and performance. The aim of this study was to investigate the progression of milking reactivity during lactation and its effects at early lactation on milk yield, milk quality, and plasma cortisol concentration in primiparous Saanen goats. The milking reactivity of 31 primiparous Saanen goats was evaluated on days 10, 30, and 60 of the lactation period by scoring step-kick behavior (SK) and head, ear, and tail movements (MOV). Milk yield, milk protein and fat percentages, somatic cell count (SCC), and plasma cortisol concentration were measured on day 10 of lactation. Goats' milking reactivity was classified as low or high (according to the SK and MOV scores measured on day 10 of lactation). SK and MOV scores decreased over the lactation period, indicating that the goats habituated to the milking procedures. Milking reactivity only affected milk fat percentage, with the less reactive goats having more milk fat than the highly reactive ones. There was no evidence of stress when goats were subjected to milking procedures during early lactation. Further studies are needed to clarify the relationships of milking reactivity with stress response and their effects on milk yield and quality. [ABSTRACT FROM AUTHOR]

This study presents data on the occurrence of aflatoxins B1, B2, G1 and G2 in maize and aflatoxin M1 (AFM1) in milk and dairy products from Serbia in 2022. A total of 100 maize samples were analysed using liquid chromatography tandem mass spectrometry, while 107 cow and goat milk, besides dairy products were tested with an ELISA method. Aflatoxins contaminated 78% of the maize samples, with aflatoxin B1 as the most prevalent aflatoxin, also at the highest determined level. All milk samples tested positive for AFM1, while contamination in dairy products ranged from 14% to 100%. The hot and dry weather during the 2022 maize growing season, characterised by moderate to extreme drought conditions, significantly increased maize contamination with aflatoxins, impacting subsequent milk and dairy products contamination. The findings of this study confirm the continuous trend and persistent challenge in Serbia concerning the prevalence of aflatoxins in maize and milk, closely linked to climate change. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. Fermented milk beverages have been known for years and are characterized by excellent health-promoting properties. Therefore, consumer attention has been drawn to this product type in recent years. In the presented research, the technology of production in laboratory and industrial scales of controlled fermentation of whey beverages containing sweet or sour organic cow or goat whey, with the addition of organic fruit juices (apple, blackcurrant, or Kamchatka berry juice), are described. Food production on a laboratory scale involves small-batch processes designed for experimentation and refinement, often with precise control over variables and conditions. In contrast, industrial-scale food production in enterprises focuses on large-volume output with an emphasis on efficiency, consistency, and adherence to regulatory standards for mass consumption. This study examined the amino acid content and nutritional value of the obtained products. Tests were carried out on fermented whey drinks' microbiology and antioxidant properties. Significance was determined using ANOVA; each prepared drink was characterized by better antioxidant properties and nutritional values compared with products without juice addition. Microbiological examination proved that only 1 product was not fit for consumption according to the Polish norm. Using whey (goat or cow) as a base for a fermented beverage with enhanced health benefits is a positive step toward using products commonly regarded as waste. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. Oligosaccharides (OS) in milk have been suggested to influence the health and development of the newborn by promoting growth of beneficial gut bacteria, stimulating brain development, and enhancing immune functions. Goat milk is a natural source of specific OS, which could be a potential beneficial ingredient for infant formula. In this study, goat milk OS (g MOS) content from ∼1,000 dairy goats across 18 commercial farms was studied. A genomic relationship matrix was used to unravel genetic and environmental factors shaping g MOS content. The most abundant g MOS identified was 3′-N-glycolyl-neuraminyl-lactose (NGL), with a concentration of 32.05 mg/kg, whereas 3-fucosyllactose (FL) exhibited the lowest concentration at 1.85 mg/kg. Acidic OS had a notably higher content (81.67 mg/kg) than neutral OS (24.88 mg/kg). High variability in g MOS content was observed among individual goats, which could for a large extent be attributed to genetic differences. Heritability estimates ranged from 31% for 3′-galactosyllactose (GL) to 85% for 3-FL. High positive genetic correlations (>0.57) were estimated between 3′-sialyllactose (SL) and 6′-SL, and between 6′-GL and 3′-GL. The contribution of differences between farms to variation in milk OS content varied from 3% for 3′-NGL to 45% for 6′-SL. Although g MOS such as 3′-GL, 6′-GL, and 6′-NGL, were significantly influenced by systematic environmental factors such as the lactation stage, the effect of these factors was relatively minor compared with the importance of genetic and farm effects. This research, which stands out due to its relatively large sample size, underscores the pivotal role of genetics, and to a smaller extent farm practices such as feed ration, in determining g MOS composition. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. There is growing interest in the origin, preparation, and application of bioactive peptides. This study investigated the effect of 6 enzymes on the structural, physicochemical properties, antioxidant activities, and antidiabetic potential of defatted fresh goat milk. Structural and functional changes resulting from enzymatic hydrolysis were assessed using gel electrophoresis, laser particle size analysis, multi-spectroscopy, and evaluations of foaming and emulsification properties. Antioxidant capacity was determined through free radical scavenging, Fe2+ chelation, and reducing ability experiments. Additionally, the inhibitory effects of the hydrolysates on α-glucosidase and α-amylase were measured to evaluate antidiabetic activity. Results showed that enzymatic hydrolysis disrupted the spatial structure of goat milk protein and reduced its molecular weight. Papain hydrolysate exhibited the highest degree of hydrolysis (32.87% ± 0.11%) and smallest particle size (294.75 ± 3.33 nm), followed by alcalase hydrolysate (29.12% ± 0.09%, 302.03 ± 7.28 nm). Alcalase hydrolysate showed the best foaming properties, and papain hydrolysate demonstrated the strongest 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radical scavenging activity, Fe2+ chelation, and antidiabetic potential. These findings provide a solid theoretical basis for utilizing defatted goat milk as functional ingredients or excipients in the food, medical, and cosmetic industries. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. Bioactive peptides produced via enzymatic hydrolysis have been widely investigated for their dipeptidyl peptidase-IV (DPP-IV) inhibitory properties. However, the deficit of studies on fermentation as a means to produce DPP-IV inhibitory peptides prompted us to draw a comparative study on DPP-IV inhibitory peptides generated from cow, camel, goat, and sheep milk using probiotic fermentation. Further, peptide identification, in silico molecular interactions with DPP-IV, and ensemble docking were performed. Results suggested that goat milk consistently exhibited greater degrees of hydrolysis than other milk types. Further, Pediococcus pentosaceus (PP-957) emerged as a potent probiotic, with significantly lower median inhibitory concentration values of DPP-IV, of 0.17, 0.12, and 0.25 µg/mL protein equivalents in fermented cow, camel, and goat milk, respectively. Overall, peptides (RPPPPVAM, CHNLDELKDTR, and VLSLSQPK) exhibited strong binding affinity, with binding energies of −9.31, −9.18, and −8.9 kcal/mol, respectively, suggesting their potential role as DPP-IV inhibitors. Overall, this study offers valuable information toward antidiabetic benefits of fermented milk products via inhibition of DPP-IV. [Display omitted] [ABSTRACT FROM AUTHOR]

This study was conducted to identify and characterize the Bacillus strain from Goat's milk in Algeria and to evaluate the prebiotic effect of H. scoparia aqueous extract and ZnO-NPs on the isolated Bacillus strain. Potentially probiotic bacteria were isolated and identified based on phenotypic characteristics, including the morphological, physiological (growth temperature, salt tolerance), biochemical (API 10S strip kits), and confirmed by 16S rDNA gene sequencing. Bacillus pumilus specie of lactic acid bacteria were identified. The base pair length of amplified DNA for the isolate was 1500 Bp. The isolates could not grow at 10°C and 45°C and tolerate up to 6% salt. H. scoparia extract and ZnO-NPs have a beneficial effect on the Bacillus pumilus strain for acid tolerance, bile tolerance, and cell surface hydrophobicity respectively; H. scoparia extract and ZnO-NPs results are high comparing to the control group's results. As the concentration of the tested samples increases, the percentage of probiotic characteristics of Bacillus pumilus increases, in contrast to the control sample. Therefore, they could be useful tools to define adequate combinations in colonic foods development and health benefits. The beneficial physiological and biochemical properties of isolated Bacillus pumilus space from Goat's milk with the H. scoparia aqueous extract and ZnO-NPs revealed their potential applications in the food industry. [ABSTRACT FROM AUTHOR]

Contagious agalactia is an infectious disease of small ruminants caused by several Mycoplasma species with leading role of M. agalactiae. Clinical signs are manifested by mastitis, arthritis, keratoconjunctivitis, abortion, pneumonia and septicaemia often in various combinations. The aim of the study was to tested milk samples from sheep and goats in herds with different health status by a rapid, sensitive, specific method for the detection and quantification of DNA of M. agalactiae. A total of 67 milk samples from sheep and goats were tested by real-time polymerase chain reaction (qPCR). Thirteen were positive for DNA and 54 were negative. The present study is the first report of detection of M. agalactiae DNA in Bulgaria. [ABSTRACT FROM AUTHOR]

Background/Objectives: The increasing occurrence of extended-spectrum β-lactamase (ESBL)–producing Enterobacterales, most commonly Escherichia coli, has become a serious problem. The aim of this study was to determine the presence of ESBL-producing Gram-negative bacteria in dairy cattle, goat and sheep farms located in southern Türkiye. Methods: Samples (409 quarter milk samples and 110 fresh faecal samples from cattle, 75 bulk tank milk samples and 225 rectal swab samples from goats and sheep) were subjected to selective isolation on MacConkey agar with ceftazidime (2 µg/mL). Isolates were identified by MALDI-ToF MS. The antimicrobial susceptibility profile of the isolates was determined by the broth microdilution method. To obtain a deeper insight into the genetic diversity of isolates substantially contributing to an efficient spread of their ESBL-determinants (23-MO00001: an E. coli from mastitis and 23-MO00002 Citrobacter freundii), the transmission potential and the genetic background of the plasmid carrying the blaCTX-M determinant was studied with whole genome analysis using Illumina sequencing. Results: Of the samples tested, 47 from the bovine faecal samples, 1 from the subclinical mastitis milk sample, 9 from the goat/sheep rectal swab samples and 5 from the goat/sheep bulk tank milk samples had ceftazidime-resistant Gram-negative strains with the ESBL phenotype. Of the 33 ESBL-producing E. coli isolates, 66.6% were resistant to tetracycline, 57.6% to sulfamethoxazole, 48.9% to nalidixic acid, 42.4% to ciprofloxacin and 33.3% to trimethoprim. Pulsed field gel electrophoresis (PFGE) results showed that the majority of E. coli isolates (16/33) and all Enterobacter spp. isolates (n = 5) were not clonally related (80% similarity cut value). The sequenced strains were observed to efficiently transfer their ceftazidime resistance to the recipient strain E. coli J53 at 37 °C (transfer rates: 101–102 transconjugants per donor cell). S1-PFGE showed that the transconjugants J53(p23MO01-T1) and J53(p23MO02-T1) had acquired plasmids of about 82 kb and 55 kb plasmids, respectively. According to WGS results, the E. coli isolate was assigned to ST162, while the C. freundii isolate was assigned to ST95. Conclusions: This study demonstrates that dairy animals are reservoirs of ESBL-producing bacteria. [ABSTRACT FROM AUTHOR]

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Nutrition is considered the primary paragenetic factor influencing the milk urea (MU) concentration in ruminants. In addition to diet, various other factors (including season, milk yield, stage of lactation, etc.) have also been shown to affect the urea concentration in ruminant milk. While the physiological concentration of MU has been well-defined for cow's milk, with an optimal range of 15 to 30 mg/dl, such parameters have yet to be established for goat's milk. The objective of this study was to determine the urea concentration in the milk of Alpine goats and to assess its correlation with the daily milk yield and the contents of fat, protein, lactose, and solids-not-fat (SNF). A total of 3,009 samples of Alpine goat milk, collected from two farms in 2021, were analyzed. The average MU concentration was 37.3 ± 8.9 mg/dl, with a range from 13.8 to 110.7 mg/dl. The average daily milk yield was 1.15 ± 0.49 kg, whereas the average milk fat and protein contents were 3.28 ± 0.97% and 3.35 ± 0.50%, respectively. The study found a positive and statistically significant correlation between the MU concentration and the contents of milk fat, protein, and SNF. Conversely, an increase in the MU concentration was negatively correlated with the daily milk yield. Monitoring the MU concentration in dairy goat herds enables the optimization of feed rations, particularly their protein and energy contents, thus improving nitrogen utilization in milk production. [ABSTRACT FROM AUTHOR]

The present study aimed to evaluate the effects of incorporating different concentrations (1% and 2%) of Malvaviscus arboreus flower (FE) and leaf (LE) extracts as functional ingredients in goat milk yogurt. This study analyzed the impact of these formulations (YFE1%, YFE2%, YLE1%, and YLE2%) on the physicochemical, bioactive, antioxidant, rheological, textural, and sensory properties of goat yogurt over a 28-day storage period. Including FE and LE extracts significantly enhanced the yogurt's antioxidant activity, reaching up to 10.17 µmol TEAC/g, and strengthened its ability to inhibit lipid oxidation during storage. This study also observed a reduction in the viability of lactic acid bacteria, particularly L. delbrueckii subsp. bulgaricus, suggesting that the extracts may have antimicrobial properties. Notably, using FE, especially at a concentration of 2% (YFE2%), improved both antioxidant and textural properties while reducing syneresis by the end of the storage period. Sensory evaluations showed positive results for YFE1% and YFE2% formulations. These findings suggest that FE has significant potential as a functional food ingredient. This research lays the groundwork for future studies exploring the integration of Malvaviscus arboreus-based ingredients into functional food products, opening new possibilities for innovation in this field. [ABSTRACT FROM AUTHOR]

Goat milk butter, locally known as "Dhan", from the Sfisfa region of Algeria, holds significant cultural and economic value. This study investigates the probiotic properties of lactic acid bacteria (LAB) present in Dhan, focusing particularly on Lactobacillus strains. Molecular identification using 16S rRNA revealed a dominance of Levilactobacillus brevis and Lactiplantibacillus plantarum, forming a substantial part of the bacterial profile. Three LAB isolates (DC01-A, DC04, and DC06) were selected from fresh samples, and rigorous analyses were performed to evaluate their probiotic properties. Safety assessments confirmed the absence of gelatinase, DNase, and haemolytic activities in all isolates. The isolates demonstrated high tolerance to bile salts and acidic conditions, along with the ability to survive simulated gastrointestinal digestion. Notably, strain DC06 exhibited exceptional survival at low pH (1.5) and high bile salt concentrations (0.15–0.3%). All isolates showed substantial growth in MRS medium with 2% phenol, although growth was significantly decreased at 5% phenol. Furthermore, our strains exhibited high adhesion rates to various solvents, demonstrating their potential for strong interaction with cell membranes. Specifically, adhesion to chloroform was observed at 98.26% for DC01-A, 99.30% for DC04, and 99.20% for DC06. With xylene, the adhesion rates were 75.94% for DC01-A, 61.13% for DC04, and 76.52% for DC06. The LAB strains demonstrated impressive growth in ethanol concentrations up to 12%, but their tolerance did not exceed this concentration. They also exhibited robust growth across temperatures from 10 °C to 37 °C, with strains DC04 and DC06 able to proliferate at 45 °C, though none survived at 50 °C. Additionally, the isolates showed significant resistance to oxidative stress induced by hydrogen peroxide (H2O2) and displayed medium to high autolytic activity, with rates of 50.86%, 37.53%, and 33.42% for DC01-A, DC04, and DC06, respectively. The cell-free supernatant derived from strain DC04 exhibited significant antimicrobial activity against the tested pathogens, while strain DC06 demonstrated moderate antioxidant activity with the highest DPPH scavenging rate at 68.56%, compared to the probiotic reference strain LGG at 61.28%. These collective findings not only suggest the probiotic viability of LAB strains found in Dhan but also highlight the importance of traditional food practises in contributing to health and nutrition. Consequently, this study supports the potential of traditional Dhan butter as a functional food and encourages further exploration of its health benefits. [ABSTRACT FROM AUTHOR]

In the context of climate change, there has been an increased interest in improving management practices for animals genetically adapted to extreme environmental conditions, such as buffaloes. The temperature–humidity index (THI) is used to determine the severity of heat stress in livestock. This study aimed to evaluate the cytokine profile, oxidative staus, differential somatic cell count (DCC), and the surface expression and activity of myeloperoxidase (MPO) in the somatic cells (SCs) of buffalo. Milk samples (n = 216) were collected from the spring to summer season under three different THI classes (THI < 72; ≤72 THI < 76, and THI ≥ 76). The cytokine profile was determined using ELISA, and the expression of DSCC and MPO was determined by flow cytometry. MPO activity was performed on SC extracts using a specific ELISA kit. Oxidative status was determined by the antioxidant/oxidant balance combining the free radical scavenging activity levels, and reactive oxygen and nitrogenous species. The results on the cytokine profile showed that at the THI ≥ 76 the levels of both IL-10 and IFN-γ were highest. IL-1β secretion was lower at the THI < 72 than at the THI values ranging from ≤72 THI < 76. Higher levels of both TNF-α and IL-12 were registered in both THI < 72 and THI ≥ 76 classes. The level of IL-4 was higher in the THI ≥ 76 class than in the ≤72 to <76 range. Data on DCC showed a decrease in the percentage of macrophages and lymphocytes as the THI increased from the ≤72 to <76 range to THI ≥ 76. Furthermore, the highest percentage of polymorphonuclear leukocyte (PMNLs) was registered in both ≤72 to <76 and THI ≥ 76 classes. The MPO activity and surface expression on SC were lower at a THI above 76, which could be associated with an absence of inflammation. A condition of oxidative imbalance was registered as demonstrated by the lower levels of antioxidant/oxidant balance along with increasing THI. Present data demonstrated that buffaloes were able to modulate the alteration of immune response activated by heat stress throughout a series of cross-linked mechanisms involving cytokine networks, different somatic cell distribution, and oxidative status. [ABSTRACT FROM AUTHOR]

Goat breeding in Peru is one of the main activities of smallholders. Goats are distributed in different agroecological zones and regions of the country, developing under heterogeneity of production systems, making it difficult to understand goat breeders' socioeconomic, technological, and productive situations. This study aimed to characterize and typify the goat production systems in the highlands of southeast Peru. A survey was conducted with 91 goat farmers from five districts of Ayacucho, Peru, using a structured and individualized questionnaire administered on their farms. The socio-economic, productive, and commercial characteristics of the goat production systems were recorded. A multiple correspondence analysis (MCA) and hierarchical classification analysis (HCA) were performed to establish a typology of the smallholders. The results reveal that the breeding system is extensive, where there is no breeding program, with natural pastures and crop stubble being the source of food for the herds. Only slightly more than half (54%) carry out a deworming program. Farmers were categorized into three different groups, corresponding to three different farming systems: Group 1 farmers raised goats solely for home consumption; Group 2 breeders raised goats for both consumption and marketing of surplus products (cheese, milk, and meat), and Group 3 farmed focused on producing cheese and goat kids and selling to local markets. The study provides valuable insights that will help design effective breeding strategies to develop sustainable goat farming in the region, considering different production systems and their respective socio-economic and trade dynamics. This classification will be essential for tailoring development programs to the specific needs of each group, promoting better use of resources, improving productivity, and enhancing the livelihoods of smallholder goat producers in Peru. [ABSTRACT FROM AUTHOR]

This study was designed to investigate the effects of adding a novel yeast culture, Saccharomyces cerevisiae refermented sorghum distiller's dried grains with solubles (SSDDGS), to the diets of lactating female goats on lactation performance and lamb growth performance. We divided 10 lactating Dazu black goats of similar age, weight, and offspring into two groups: one fed a pelleted diet with 50 g/day SSDDGS (ET), and the other without SSDDGS as a control (EC) for 7 weeks. We monitor the weight changes of each goat and collect blood and milk samples from experimental ewes at specific times for hormone and milk composition determination. We use ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to detect metabolites in the serum of lactating ewes. Our results showed that SSDDGS supplementation significantly reduced female goats' average daily weight loss during weeks 2–4 of lactation and increased serum IGF-1 and prolactin levels at week 4 (p < 0.05). SSDDGS supplementation in early lactation significantly increased milk protein, lactose, and ash content (p < 0.05). UPLC-MS/MS analysis showed that SSDDGS changed the levels of 58 metabolites in the serum of lactating goats. These metabolites were mainly involved in the sohingolipid signaling pathway, and cysteine, methionine, and sphingolipid metabolism. In summary, Yeast culture SSDDGS reduced weight loss, enhanced milk quality, and modified metabolic profiles in early lactation goats, providing insight into the potential regulatory role and mechanism of yeast culture in lactation female goats. [ABSTRACT FROM AUTHOR]

The aim of this research was to investigate the effects of adding moringa polysaccharides (MOP) on the growth performance, immune function, rumen tissue morphology, and rumen microbial community in early-weaned goat kids. Twenty-one 7-day-old Leizhou male goat kids weighing (3.05 ± 0.63) kg, were randomly divided into a control group (CON group), a low-dose group (LOW group), and a high-dose group (HIG group). MOP was added to the goat kids' milk replacer (MR) at 0, 0.15, and 0.3% (on dry matter basis),fed until 60 days of age, and four goat kids in each group with body weights close to the mean of each group were selected for slaughter. The results showed that, compared to the CON group, the MOP groups significantly improved final body weight, body measurements, daily weight gain, and feed intake of the early weaned goat kids; significantly reduced the content of propionic acid, butyric acid, valeric acid, and ammoniacal nitrogen; and in addition, the addition of MOP could significantly increase the height of rumen nipple, the content of immunoglobulin G (IgG) in the serum. The HIG group significantly increased rumen pH, rumen muscularis layer thickness, rumen wall thickness, and serum immunoglobulin A (IgA), and immunoglobulin M (IgM). In conclusion, the addition of MOP positively impacted the growth performance, serum immune function, and rumen tissue morphology in early-weaned goat kids. [ABSTRACT FROM AUTHOR]

The article "Linking Macronutrient Composition of Common Allergenic Foods to European and North American Food Allergy Prevalence" explores the relationship between macronutrient profiles of common allergenic foods and food allergy prevalence in Europe and North America. The study found that higher protein and glycine contents in allergenic foods were negatively correlated with food allergy prevalence, while carbohydrate and fat contents showed no association. The research suggests that total protein and glycine contents in common allergenic foods may influence food allergy development, prompting further investigation into the role of macronutrients in food allergies. [Extracted from the article]

Background/Objectives: Among US breastfeeding women, those with obesity have significantly increased breast milk fat and caloric content from foremilk to hindmilk, with a 4-fold increase in fat content from the first to last milk sample. In view of different dietary norms and nutritional standards, we sought to evaluate the relationship between maternal BMI with breast milk fat and calorie content in women from Brazil, a low–middle-income country. Methods: Women who delivered singleton-term neonates were recruited from the Ana Abrao Breastfeeding Center (AABC) and Human Milk Bank at the Federal University of Sao Paulo, Brazil. These women were then studied at 7–8 weeks postpartum. Women were grouped by BMI categories of nonobese (NonOB; BMI 18.5–29.9) and obese (OB; BMI ≥ 30). A breast pump was applied, and milk samples were obtained continuously in 10 mL aliquots from foremilk to hindmilk; samples were analyzed for macronutrients and lipids, and maternal blood was analyzed for serum lipids and glucose. Results: As compared to NonOB women, those with OB had significantly higher milk fat in the mid (4.9 ± 0.3 vs. 3.9 ± 0.2) and last hindmilk (6.6 ± 0.4 vs. 5.5 ± 0.3) samples, though not in the first foremilk sample, as compared to NonOB women. In both NonOB and OB subjects, milk caloric and fat content increased 1.5 to 2-fold from foremilk to hindmilk, with the average milk caloric value being 11% greater in OB women. Protein content was significantly increased in all three milk samples (first, middle, and last) in women with OB. Conclusions: Although the value of breastfeeding remains clear, these findings may have significant implications for infant nutrition and excessive infant weight gain in women with OB. [ABSTRACT FROM AUTHOR]