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The Back Propagation (BP) neural network has the problems of low accuracy and poor convergence in the process of binocular camera calibration. A method based on BP neural network optimized by improved genetic simulated annealing algorithm (IGSAA-BP) is proposed to solve these problems to complete the binocular camera calibration. The method of combining Gaussian scale space and Harris corner detection operator is used for corner detection. A matched algorithm of homonymous corner is proposed by combining point-to-point spatial mapping and grid motion statistics. The pixel values of the homonymous corner and three-dimensional coordinate values are taken as the input and output of BP neural network respectively. The crossover and mutation probability of genetic simulated annealing algorithm and the annealing criterion are improved, the IGSAA-BP neural network is used to calibrate the binocular camera. The average calibration accuracy of BP neural network and IGSAA-BP neural network is 0.71mm and 0.03mm, respectively. The average calibration accuracy of binocular camera is improved by 96%. The iteration speed is increased by 20 times and global optimization ability is improved. It can be seen that the IGSAA-BP neural network can improve the calibration accuracy of binocular camera and accelerate convergence speed.