1. Effect of nitrate, ammonium, and urea ratios and molybdenum level in the nutrient solution on vegetative growth and biochemical responses of basil plants.
- Author
-
Mirmoghadam, Sajad, Eshghi, Saeid, Etemadi, Mohammad, and Jamali, Babak
- Subjects
- *
NITRATE reductase , *AMMONIUM nitrate , *BASIL , *FACTORIAL experiment designs , *PLANT cells & tissues - Abstract
In hydroponics, preferring a higher nitrate to ammonia ratio can lead to nitrate accumulation in plant tissues. Reducing nitrate application and substituting nitrate-based fertilizers with ammonium or urea can help solve this issue. Additionally, adding molybdenum (Mo) can decrease nitrate accumulation by stimulating nitrate reductase activity. This study was carried out in a factorial design within a completely randomized experimental setup to investigate the effects of varying nitrate, ammonium, and urea ratios, as well as Mo concentration in the nutrient solution, on vegetative growth and biochemical parameters in hydroponically grown Jenovise basil. The treatments included different ratios of nitrate: ammonium: urea (75:25:0, 50:25:25, 25:25:50, 0:25:75, 50:0:50, 25:0:75, 0:0:100, or 100:0:0) and three Mo levels (0.001, 0.01, 0.1 mg. L−1) in a modified Hoagland solution. The results indicated that growth-associated characteristics improved when nitrate was partially substituted with ammonium and urea in the nutrient solution. The highest total phenolics (2.61 mg. g−1 DW) and flavonoids (2500.36 mg. g−1 DW) were observed at a nitrate: ammonium: urea ratio of 75:25:0 with 0.1 mg. L−1 Mo. Plants treated with a nutrient solution containing a nitrate: ammonium: urea ratio of 0:25:75 and 0.01 mg L−1 Mo showed a significant increase in chlorophyll b concentration, reaching 0.22 mg.g−1 FW, which was higher than all other treatments. In conclusion, our findings demonstrated that optimizing the combined nitrate, ammonium, and urea ratios along with Mo in the nutrient solution regulated nitrate accumulation and growth in hydroponically grown basil. [ABSTRACT FROM AUTHOR]
- Published
- 2025
- Full Text
- View/download PDF