1. High Cholesterol Diet-Induced Changes in Oxysterol and Scavenger Receptor Levels in Heart Tissue
- Author
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Ali Sahin, Nesrin Kartal Ozer, Burak Yazgan, Erdi Sozen, Umit Ince, Sozen, Erdi, Yazgan, Burak, Sahin, Ali, Ince, Umit, Ozer, Nesrin Kartal, Sozen, E., Department of Biochemistry, Faculty of Medicine, Genetic and Metabolic Diseases Research and Investigation Center (GEMHAM), Marmara University, 34854 Maltepe, Istanbul, Turkey -- Yazgan, B., Central Research Laboratory, Amasya UniversityAmasya 05100, Turkey -- Sahin, A., Department of Biochemistry, Faculty of Medicine, Genetic and Metabolic Diseases Research and Investigation Center (GEMHAM), Marmara University, 34854 Maltepe, Istanbul, Turkey -- Ince, U., Acıbadem University and Acıbadem Health Group, Istanbul, Turkey -- Ozer, N.K., Department of Biochemistry, Faculty of Medicine, Genetic and Metabolic Diseases Research and Investigation Center (GEMHAM), Marmara University, 34854 Maltepe, Istanbul, Turkey, Acibadem University Dspace, ince, umit -- 0000-0002-6113-661X, Sahin, Ali -- 0000-0001-5594-1551, and [Sozen, Erdi -- Sahin, Ali -- Ozer, Nesrin Kartal] Marmara Univ, Genet & Metab Dis Res & Invest Ctr GEMHAM, Fac Med, Dept Biochem, TR-34854 Istanbul, Turkey -- [Yazgan, Burak] Amasya Univ, Cent Res Lab, TR-05100 Amasya, Turkey -- [Ince, Umit] Acibadem Univ, Istanbul, Turkey -- [Ince, Umit] Acibadem Hlth Grp, Istanbul, Turkey
- Subjects
0301 basic medicine ,CD36 Antigens ,Male ,Aging ,CD36 ,Biochemistry ,DISEASE ,chemistry.chemical_compound ,Tandem Mass Spectrometry ,OXIDATIVE STRESS ,Ketocholesterols ,VITAMIN-E ,Liver X Receptors ,chemistry.chemical_classification ,Receptors, Scavenger ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,lcsh:Cytology ,HYPERCHOLESTEROLEMIA-INDUCED ATHEROSCLEROSIS ,ALPHA-TOCOPHEROL ,General Medicine ,Oxysterols ,ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS ,Cholesterol ,lipids (amino acids, peptides, and proteins) ,Rabbits ,FATTY-ACIDS ,InformationSystems_MISCELLANEOUS ,Oxidation-Reduction ,Research Article ,medicine.medical_specialty ,Oxysterol ,Article Subject ,Blotting, Western ,METABOLISM ,DENSITY-LIPOPROTEIN ,High cholesterol ,Gas Chromatography-Mass Spectrometry ,03 medical and health sciences ,Internal medicine ,medicine ,Animals ,Scavenger receptor ,lcsh:QH573-671 ,Triglycerides ,LXR-ALPHA ,Myocardium ,ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS ,Fatty acid ,Lipid metabolism ,Cell Biology ,medicine.disease ,Lipid Metabolism ,ENDOTHELIAL-CELLS ,Hydroxycholesterols ,030104 developmental biology ,Endocrinology ,ComputingMethodologies_PATTERNRECOGNITION ,chemistry ,ABCA1 ,biology.protein ,Lipid Peroxidation - Abstract
PubMed ID: 30008986, Involvement of high cholesterol and oxidative stress in cardiovascular diseases is well studied, as it can be hypothesized that various products originated from lipid peroxidation, such as oxysterols, or affected protein expression might lead to cardiomyocyte damage followed by the pathological modifications. Although oxidation of excessive cholesterol to oxysterols in elevated stress conditions is identified by a number of studies, the role of a high cholesterol diet in regulating fatty acid and oxysterol accumulation, together with scavenger receptor mRNA levels, in the heart remains little investigated. Our study provides a detailed analysis of the changes in fatty acid, oxysterol, and scavenger receptor profiles and its relation with histological alterations in the heart tissue. We evaluated alterations of fatty acid composition, by the GC-MS method, while 4ß-, 25-, and 27-hydroxycholesterol and 7-ketocholesterol levels by means of LC-MS/MS in high cholesterol diet-fed rabbits. Additionally, a number of proteins related to lipid metabolism and scavenger receptor mRNA expressions were evaluated by Western blotting and RT-PCR. According to our in vivo results, a high cholesterol diet enhances a number of unsaturated fatty acids, oxysterols, and LXR?, in addition to CD36, CD68, CD204, and SR-F1 expressions while ?-tocopherol supplementation decreases LXR? and SR expressions together with an increase in 27-hydroxycholesterol and ABCA1 levels. Our results indicated that the high cholesterol diet modulates proteins related to lipid metabolism, which might result in the malfunction of the heart and ?-tocopherol shows its beneficial effects. We believe that this work will lead the generation of different theories in the development of heart diseases.
- Published
- 2018