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2 results on '"ionomic profiling"'

1. Different Reactive Oxygen Species Lead to Distinct Changes of Cellular Metal Ions in the Eukaryotic Model Organism Saccharomyces cerevisiae.

Author

Wu, Ming J., O'Doherty, Patrick J., Murphy, Patricia A., Lyons, Victoria, Christophersen, Melinda, Rogers, Peter J., Bailey, Trevor D., and Higgins, Vincent J.

Subjects
*REACTIVE oxygen species, *IONIZATION of gases, *SACCHAROMYCES cerevisiae, *HYDROGEN peroxide, *LINOLEIC acid, *YEAST
Abstract

Elemental uptake and export of the cell are tightly regulated thereby maintaining the ionomic homeostasis. This equilibrium can be disrupted upon exposure to exogenous reactive oxygen species (ROS), leading to reduction or elevation of the intracellular metal ions. In this study, the ionomic composition in the eukaryotic model organism Saccharomyces cerevisiae was profiled using the inductively-coupled plasma optical emission spectrometer (ICP-OES) following the treatment with individual ROS, including hydrogen peroxide, cumen hydroperoxide, linoleic acid hydroperoxide (LAH), the superoxide-generating agent menadione, the thiol-oxidising agent diamide [diazine-dicarboxylic acid-bis(dimethylamide)], dimedone and peroxynitrite. The findings demonstrated that different ROS resulted in distinct changes in cellular metal ions. Aluminium (Al3+) level rose up to 50-fold after the diamide treatment. Cellular potassium (K+) in LAH-treated cells was 26-fold less compared to the non-treated controls. The diamide-induced Al3+ accumulation was further validated by the enhanced Al3+ uptake along the time course and diamide doses. Pre-incubation of yeast with individual elements including iron, copper, manganese and magnesium failed to block diamide-induced Al3+ uptake, suggesting Al3+-specific transporters could be involved in Al3+ uptake. Furthermore, LAH-induced potassium depletion was validated by a rescue experiment in which addition of potassium increased yeast growth in LAH-containing media by 26% compared to LAH alone. Taken together, the data, for the first time, demonstrated the linkage between ionomic profiles and individual oxidative conditions. [ABSTRACT FROM AUTHOR]

Published
2011
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2. Different Reactive Oxygen Species Lead to Distinct Changes of Cellular Metal Ions in the Eukaryotic Model Organism Saccharomyces cerevisiae

Author

Patricia A Murphy, Melinda Christophersen, Peter J. Rogers, Patrick J. O'Doherty, Ming J. Wu, Trevor D. Bailey, Vincent J. Higgins, and Victoria Lyons

Subjects
Models, Molecular, Potassium, yeast, medicine.disease_cause, lcsh:Chemistry, chemistry.chemical_compound, Menadione, Benzene Derivatives, Magnesium, Hydrogen peroxide, lcsh:QH301-705.5, Spectroscopy, Diamide, chemistry.chemical_classification, reactive oxygen species, biology, Vitamin K 3, General Medicine, Oxidants, Computer Science Applications, Linoleic Acids, Biochemistry, Saccharomyces cerevisiae, Lipid Peroxides, chemistry.chemical_element, Oxidative phosphorylation, Article, Catalysis, Inorganic Chemistry, Peroxynitrous Acid, medicine, Physical and Theoretical Chemistry, Molecular Biology, Ions, Manganese, Reactive oxygen species, Cyclohexanones, Organic Chemistry, metal ions, Hydrogen Peroxide, biology.organism_classification, Yeast, Oxidative Stress, chemistry, lcsh:Biology (General), lcsh:QD1-999, ionomic profiling, Copper, Oxidative stress, Aluminum
Abstract

Elemental uptake and export of the cell are tightly regulated thereby maintaining the ionomic homeostasis. This equilibrium can be disrupted upon exposure to exogenous reactive oxygen species (ROS), leading to reduction or elevation of the intracellular metal ions. In this study, the ionomic composition in the eukaryotic model organism Saccharomyces cerevisiae was profiled using the inductively-coupled plasma optical emission spectrometer (ICP-OES) following the treatment with individual ROS, including hydrogen peroxide, cumen hydroperoxide, linoleic acid hydroperoxide (LAH), the superoxide-generating agent menadione, the thiol-oxidising agent diamide [diazine-dicarboxylic acid-bis(dimethylamide)], dimedone and peroxynitrite. The findings demonstrated that different ROS resulted in distinct changes in cellular metal ions. Aluminium (Al(3+)) level rose up to 50-fold after the diamide treatment. Cellular potassium (K(+)) in LAH-treated cells was 26-fold less compared to the non-treated controls. The diamide-induced Al(3+) accumulation was further validated by the enhanced Al(3+) uptake along the time course and diamide doses. Pre-incubation of yeast with individual elements including iron, copper, manganese and magnesium failed to block diamide-induced Al(3+) uptake, suggesting Al(3+)-specific transporters could be involved in Al(3+) uptake. Furthermore, LAH-induced potassium depletion was validated by a rescue experiment in which addition of potassium increased yeast growth in LAH-containing media by 26% compared to LAH alone. Taken together, the data, for the first time, demonstrated the linkage between ionomic profiles and individual oxidative conditions.

Published
2011

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