1. Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation
- Author
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Jonathan P. May, Eno Hysi, Shyh-Dar Li, Lauren A. Wirtzfeld, Elijus Undzys, and Michael C. Kolios
- Subjects
0301 basic medicine ,Effective size ,Materials science ,Oxygen saturation ,lcsh:QC221-246 ,Photoacoustic imaging in biomedicine ,01 natural sciences ,010309 optics ,03 medical and health sciences ,Mild hyperthermia ,0103 physical sciences ,Spectral slope ,medicine ,lcsh:QC350-467 ,Radiology, Nuclear Medicine and imaging ,Tumor blood vessels ,Photoacoustic and ultrasound tissue characterization ,Tumor Oxygenation ,lcsh:QC1-999 ,Atomic and Molecular Physics, and Optics ,3. Good health ,Cancer treatment ,Red blood cell ,030104 developmental biology ,medicine.anatomical_structure ,Cancer treatment monitoring ,lcsh:Acoustics. Sound ,Radiofrequency analysis ,lcsh:Physics ,lcsh:Optics. Light ,Research Article ,Biomedical engineering ,Blood vessel - Abstract
Frequency analysis of the photoacoustic radiofrequency signals and oxygen saturation estimates were used to monitor the in-vivo response of a novel, thermosensitive liposome treatment. The liposome encapsulated doxorubicin (HaT-DOX) releasing it rapidly (Photoacoustic imaging (VevoLAZR, 750/850 nm, 40 MHz) of EMT-6 breast cancer tumors was performed 30 min pre- and post-treatment and up to 7 days post-treatment (at 2/5/24 h timepoints). HaT-DOX-treatment responders exhibited on average a 22% drop in oxygen saturation 2 h post-treatment and a decrease (45% at 750 nm and 73% at 850 nm) in the slope of the normalized PA frequency spectra. The spectral slope parameter correlated with treatment-induced hemorrhaging which increased the optical absorber effective size via interstitial red blood cell leakage. Combining frequency analysis and oxygen saturation estimates differentiated treatment responders from non-responders/control animals by probing the treatment-induced structural changes of blood vessel.
- Published
- 2022