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Different extraction techniques were used to exploit fruit processing residues for their use as a source of phenolic compounds. Three different extraction methods, namely microwave-assisted extraction (MAE), subcritical water extraction (SWE), and maceration (M), were assessed to gauge their respective efficacies. Total phenolic content (TPC) and total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and radical scavenging activity ABTS assay were evaluated. High-performance liquid chromatography was used to assess the polyphenolic profile. MAE was the extraction technique that allowed the highest recovery of polyphenolic compounds. Concerning the fruit by-products analyzed, the extract of pomegranate peels obtained using M60C and MAE had the highest TPC (313 ± 24 mg GAE/g dry weight (dw)) and TFC (36.0 ± 2.8 mg EE/g dw), respectively, and the highest antioxidant activity (FRAP = 740 ± 67 mg AAE/g dw and ABTS (628 ± 27 mg TE/g dw) corresponded to M60C. The phenolic composition obtained for this sample using high-performance liquid chromatographic–diode array detection (HPLC–DAD) showed that gallic acid, protocatechuic acid, β-resorcylic acid, (+)-cathechin, and rutin were the main phenolics found. The findings underscore the capacity of agricultural by-products to act as a source of phenolic compounds. This offers a feasible solution to enhance the nutritional content in food while simultaneously minimizing environmental waste. [ABSTRACT FROM AUTHOR]

The use of natural dyes as textile dyes is increasing due to environmentally friendly standards and does not require the use of synthetic dyes. By banning the use of synthetic dyes containing azo groups, this is the right moment to reintroduce natural dyes that have been abandoned for a long time. This study aims to identify the active compound of tantrum leaves (Indigofera tinctoria) as a natural textile dye by maceration extraction method with variations in maceration time and material treatment on wet and dry tantrum leaves. This experimental research method was carried out using several stages of analysis, namely the phytochemical test, FTIR (Fourier Transform Infrared Spectroscopy), and UV-Vis (Ultraviolet-Visible Light) spectrophotometer. The results of the phytochemical tests showed that the compounds contained in the Indigofera tinctoria plant were indigo, flavonoids, steroids, and alkaloids. The FTIR test indicated that the dye content in the extract was from the indigo group. The results of the UV-Vis spectrum showed that there was maximum absorption in wet tantrum leaf extract, dry tantrum leaf extract, and alkaline extract at wavelengths of 406.50 nm, 365.50 nm, and 664.50 nm, respectively. Natural dyes in textiles have a positive impact on the environment, the color waste products are biodegradable and can be decomposed. [ABSTRACT FROM AUTHOR]

In the present study, the optimized solvent extraction conditions with regards to the total polyphenol content (TPC) and antioxidant capacity of rosemary leaf extract (RLE) were determined. The one-factor-at-a-time method was used to independently investigate the effect of several extraction parameters, including ethanol concentration (0–100% v/v), extraction temperature (50–80°C), extraction period (15–60 min), material–solvent ratio (1:5–1:10 g/mL), and extraction cycles (1, 2, and 3 times) on polyphenol content. Response surface methodology (RSM), in combination with a central composite design, was used to perform optimization. The following optimal conditions that gave maximal TPC were determined and experimentally verified: ethanol concentration of 65% (v/v), extraction temperature of 65°C, material–solvent ratio of 1:7.5 g/mL, extraction time of 15 min, and 2 cycles of extraction. These parameters corresponded with the TPC yield of 87.42 ± 0.25 mg gallic acid equivalent/g dried feed material (mg GAE/g DW). The optimal conditions gave a high extraction yield (337 ± 6 mg dried extract/g dried feed material) with 197.28 ± 3.11 mg GAE/g dried extract. The estimated models were strongly significant (p < 0.05) for TPC values with significant regression coefficients (R 2) of 0.9979. The obtained RLE was supposed to be the top grade of natural antioxidant with the IC50 (DPPH assays) value of 9.4 ± 0.1 μg/mL, which is higher than that of the vitamin C by just three times (IC50 = 3.2 ± 0.1 μg/mL). Current results justify RLE as a potential agent in food preservation applications.

Background: Antirrhinum majus (Snapdragon) is a perennial Mediterranean-native plant that is commonly used for mass display. Few reports acknowledged the traditional use of A. majus for its medicinal and therapeutic effects. Herein, we assess the impact of A. majus’s sample preparation and extraction methods on the plant-aerial parts’ phytochemical contents and antimicrobial activity. Furthermore, the microbial targets of the extracts’ secondary metabolites are inspected using molecular docking simulations. Methods: The leaves and flowers of A. majus were prepared as fresh and air-dried samples, then extracted using cold maceration and hot reflux, respectively. Extracts with the best phytochemical profiles were selected to test their antimicrobial activities against Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli and Candida albicans. Besides, molecular docking of 66 reported isolated compounds was conducted against various microbial targets. Results: The dried-refluxed samples revealed a massive deterioration in their phytochemical profiles, whereas the macerated flowers extract exhibited the highest total phenolic content and antimicrobial activity against all tested bacterial strains. However, both flowers and leaves extracts showed similar minimum inhibitory and lethal concentrations against C. albicans. Molecular docking studies revealed that chlorogenic acid, chalcononaringenin 4’-glucoside, 3,4,2’,4’,6’-pentahydroxy-chalcone 4’-glucoside, apigenin-7-glucuronide, and luteolin-7-glucuronide were the lead compounds in expressing the antimicrobial activity. Yet, A. majus’s compounds could neither inhibit the 30S ribosomal subunit nor muramyl ligase E. Conclusion: Our results suggest that cold maceration of A. majus fresh aerial parts gave higher flavonoid and phenolic content contributing to its antimicrobial properties. These flavonoids and phenolic compounds are predicted to have a crucial role in inhibiting fungal sterol 14-demethylase, and bacterial dihydropteroate synthase and gyrase B subunit proteins.

The purpose of this study was to obtain a natural textile dye taken from the sap from the banana tree part. To obtain the yield yield of dyes and the intensity of the sap collection is done by maceration extraction process. The part of the banana tree taken by the sap is the stem, stem and fruit stalk. This research was conducted with the sorting stage, namely thinly slicing stems, midribs and fruit stalks. The results of sorting are dried in the sun for 5 days in the sun then crushed until smooth with a size of 60-80 mesh. The powder was extracted by maceration by immersing each stem, stem and fruit stalk with soaking media: aquades, methanol, ethanol and ethyl acetate for 5 days with manual stirring. The filtrate is distilled so that it gets concentrated liquid and in the oven to become a dye powder. In the powder, yield and color testing are done using a spectrophotometer UV Vis. The most optimal yield produced is on the banana fronds with methanol media is 22.8248%.. The most optimal color intensity obtained in banana fronds with ethanol maceration media is 0.505.

In the present study, the optimized solvent extraction conditions with regards to the total polyphenol content (TPC) and antioxidant capacity of rosemary leaf extract (RLE) were determined. The one-factor-at-a-time method was used to independently investigate the effect of several extraction parameters, including ethanol concentration (0–100% v/v), extraction temperature (50–80°C), extraction period (15–60 min), material–solvent ratio (1:5–1:10 g/mL), and extraction cycles (1, 2, and 3 times) on polyphenol content. Response surface methodology (RSM), in combination with a central composite design, was used to perform optimization. The following optimal conditions that gave maximal TPC were determined and experimentally verified: ethanol concentration of 65% (v/v), extraction temperature of 65°C, material–solvent ratio of 1:7.5 g/mL, extraction time of 15 min, and 2 cycles of extraction. These parameters corresponded with the TPC yield of 87.42 ± 0.25 mg gallic acid equivalent/g dried feed material (mg GAE/g DW). The optimal conditions gave a high extraction yield (337 ± 6 mg dried extract/g dried feed material) with 197.28 ± 3.11 mg GAE/g dried extract. The estimated models were strongly significant (p < 0.05) for TPC values with significant regression coefficients (R2) of 0.9979. The obtained RLE was supposed to be the top grade of natural antioxidant with the IC50 (DPPH assays) value of 9.4 ± 0.1 μg/mL, which is higher than that of the vitamin C by just three times (IC50 = 3.2 ± 0.1 μg/mL). Current results justify RLE as a potential agent in food preservation applications. [ABSTRACT FROM AUTHOR]

The aim of the study was to investigate the anti-ultraviolet properties of β-cyclodextrin-grafted cotton fabrics dyed with broadleaf holly leaf extract. Flavonoids were extracted from broadleaf holly leaf by maceration and a stoichiometry of 1:1 inclusion complex with β-cyclodextrin was formed. Characterized by the fluorescence spectrum and ultraviolet spectrophotometry, the fluorescence intensities and ultraviolet absorption of the macerated extract were enhanced by increasing the amount of cyclodextrin. Cotton fabrics were grafted with β-cyclodextrin through a crosslinking reaction based on citric acid in the presence of sodium hypophosphite then dyed with the macerated extract of broadleaf holly leaf used as a natural ultraviolet absorbent. The anti-ultraviolet property of fabrics dyed by a macerated extract was characterized in terms of the ultraviolet protection factor. It was noted that the cotton fabrics grafted with β-cyclodextrin exhibit enhanced anti-ultraviolet and wrinkle recovery properties compared to the unmodified samples and show an excellent durability against 30 washing cycles, accompanied by a loss of tensile strength. [ABSTRACT FROM AUTHOR]

Mediterranean-native perennial plant Antirrhinum majus was scrutinized in this study for its antioxidant activity and its total phenolic content in order to test for the plant's wound-healing capability. The traditional uses of this plant to treat gum scurvy, various tumors, ulcers, and hemorrhoids were the main idea behind this study. Leaves and flowers of the A. majus were extracted by maceration. Pilot qualitative phytochemical tests were made to check the presence of various secondary metabolites. Quantitatively, the flowers' macerate indicated superlative results regarding antioxidant activity and total phenolic content. However, the in vivo wound-healing capability study was made using 30 Wistar strain albino rats. This innovative part of the study revealed that the healing power of the flowers' extract ointment (5% w/w) was superior compared to the leaves' extract (5% w/w) and the positive-control ointments (MEBO) (1.5% w/w) (p ≤ 0.001). This activity was assessed by visual examination, wound-length measurement, and estimation of hydroxyproline content. Antirrhinum majus is a promising plant to be considered for wound healing. However, further testing (including histological examination and high-performance liquid chromatography (HPLC) analysis) is necessary to understand more about its mechanisms of action. [ABSTRACT FROM AUTHOR]

Ultrasonic-assisted extraction (UAE) and maceration extraction (ME) were optimized using response surface methodology (RSM) for total phenolic compounds (TPC) from fresh olives. The main phenolic compounds and antioxidant activity of TPC were also investigated. The optimized result for UAE was 22 mL/g of liquid-solid ratio, 47 °C of extraction temperature and 30 min of extraction time, 7.01 mg/g of yielding, and for ME was 24 mL/g of liquid-solid ratio, 50 °C of extraction temperature and 4.7 h of extraction time, 5.18 mg/g of yielding. The HPLC analysis revealed that the extracts by UAE and ME possessed 14 main phenolic compounds, and UAE exhibited more amounts of all phenols than ME. The most abundant phenolic compounds in olive extracts were hydroxytyrosol, oleuropein and rutin. Both extracts showed excellent antioxidant activity in a dose-dependent manner. Taken together, UAE could effectively increase the yield of phenolic compounds from olives. In addition these phenolic compounds could be used as a potential source of natural antioxidants. [ABSTRACT FROM AUTHOR]

This study aimed to identify the least aggressive and highest yield extraction method to obtain bioactive compounds from Inga marginata Willd fruits, determine the chemical components, and evaluate the extracts'' antimicrobial and cytotoxic activity. The extraction efficiency was expressed by the total phenolic and total flavonoid content and antioxidant activity (DPPH, IC 50 , and ORAC) using conventional, ultrasound, and microwave-assisted extraction. The highest bioactive compound content was achieved using 5 min at 60 °C for total phenolic content (214.98 mg GAE g−1), total flavonoid content (22.90 mg EQ g−1), DPPH (45.98 μmol TEAC g−1), inhibitory capacity (0.80 mg mL−1), and ORAC (167.25 μmol Trolox g−1) using ultrasonic extraction, and the extract inhibited the growth of all microorganisms tested. Thirteen chemical compounds were determined by ESI-ToF-MS, confirming the high phytochemical capacity of the extract. Lastly, the Inga extract showed no cytotoxicity at the concentrations used. [Display omitted] • Ultrasound-assisted extraction had greater efficiency in bioactive compound yield. • The natural Inga extract had high antioxidant and antimicrobial capacity. • The temperature significantly affected all determinations and extraction methods. • Twelve phytochemicals and one sugar were identified. • The Inga peel and pulp extract did not show toxicity at the cellular level. [ABSTRACT FROM AUTHOR]

Abstract: The present study compares the two extraction methods and evaluates the free radical scavenging activity of Acacia nilotica. Results indicated that the sequential extraction method was effective in concentrating the active principles in the ethanol extract as compared to the maceration method in DPPH assay. Based on the results, free radical scavenging property of the extracts obtained from sequential extraction method was analyzed in different assays to find out the possible antioxidant mechanism. Our results indicate that ethanol extract rich in phenolic and flavonoid contents had potent antioxidant activity and were significant in comparison with all the positive controls used in this study. The possible antioxidant mechanism of the ethanol extract can be due to its hydrogen or electron donating and direct free radical scavenging properties. Hence, the ethanol extract represents a source of potential antioxidants that could be used in pharmaceutical and food preparations. [Copyright &y& Elsevier]