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132 results on '"mass spectrum analysis"'

1. Benzenedicarboxylic acid upregulates O48814 and Q9FJQ8 for improved nutritional contents of tomato and low risk of fungal attack.

Author

Ahmad, Aqeel, Akram, Waheed, Shahzadi, Iqra, Wang, Rui, Hu, Du, Bashir, Zoobia, Jaleel, Waqar, Ahmed, Shakeel, Tariq, Wajeeha, Li, Guihua, Wu, Tingquan, Ahmad Yasin, Nasim, and Shafique, Shazia

Subjects
*PHTHALIC acid, *OCHRATOXINS, *ALTERNARIA alternata, *UBIQUITIN ligases, *PLANT metabolites, *LIGNINS, *TOMATOES
Abstract

BACKGROUND: Tomato is an important food item and a cocktail of phytonutrients. In the current study, metabolites from a non‐pathogenic fungal species Penicillium oxalicum have been exploited to obtain nutritionally augmented tomato fruits from the plants to better withstand against Alternaria alternata infection. RESULTS: Initially, bioactivity‐guided assay and chromatographic analyses identified the bioactive metabolites of P. oxalicum [benzenedicarboxylic acid (BDA) and benzimidazole]. Then, ≥3 times elevated quantities of vitamins and other nutritional elements (protein, fat, fibers, and carbohydrates) were achieved by the foliar application of BDA. The maximum increase (625.81%) was recorded in riboflavin contents; however, thiamine showed the second highest enhancement (542.86%). Plant metabolites analysis revealed that jasmonic acid contents were boosted 121.53% to significantly enhance guaiacyl lignin defenses along with the reduction in coumarin contents. The protein profile analysis explored three most actively responding protein species toward BDA applications, (i) palmitoyltransferase protein Q9FLM3; (ii) serine/threonine‐protein kinase O48814; and (iii) E3 ubiquitin‐protein ligase Q9FJQ8. The O48814 improved plant defenses; whereas, Q9FJQ8 protein was negatively regulating cysteine‐type endopeptidase activity and assisted plant to resist schedule alterations. Tomato cultivar with more active innate metabolism was found to be more responsive toward BDA. Furthermore, the bioactive compounds were enriched by using the two‐step extraction method of ethyl acetate and chloroform, respectively. CONCLUSION: Penicillium oxalicum a non‐pathogenic fungal species, produced BDA, induced nutritional contents in tomato and protected it against Alternaria alternata. The current study is the first report on the bioactivity of BDA and benzimidazole concerning the nutritional enhancement and plant defense improvement. © 2019 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2019
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2. Analysis of organic components in resin-modified pulp capping materials: critical considerations.

Author

Nilsen, Bo W., Jensen, Einar, Örtengren, Ulf, and Michelsen, Vibeke B.

Subjects
*ORGANIC compound analysis, *COMPUTED tomography, *DENTAL pulp, *GAS chromatography, *GUMS & resins, *HIGH performance liquid chromatography, *MASS spectrometry, *QUINONE, *ACYCLIC acids
Abstract

The purpose of this study was to elucidate the organic composition and eluates of three resin-based pulp-capping materials in relation to their indications and safety data sheets. Uncured samples of Theracal LC, Ultra-Blend Plus, and Calcimol LC were investigated using gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Identification/quantification of 7-d leachables of cured samples was performed using GC-MS for 2-hydroxyethyl methacrylate (HEMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), camphorquinone (CQ), ethylene glycol dimethacrylate (EGDMA), ethyl-4-(dimethylamino)benzoate (DMABEE), and triethylene glycol dimethacrylate (TEGDMA). A similar organic composition was found for Ultra-Blend and Calcimol; however, only Ultra-Blend is indicated for direct pulp-capping. In contrast to the other materials analysed, Theracal contained substances of high molecular weight. The safety data sheets of all materials were incomplete. We detected HEMA, CQ, and TEGDMA in eluates from Ultra-Blend and Calcimol, and it was considered that HEMA might have originated from decomposition of diurethane dimethacrylate (UDMA) in the GC-injector. For Theracal, additives associated with light curing (DMABEE and CQ) were detected in higher amounts (4.11 and 19.95 μg mm−2) than in the other materials. Pores were quantified in all samples by micro-computed tomography (micro-CT) analysis, which could influence leaching. The organic substances in the investigated materials might affect their clinical suitability as capping agents, especially for direct capping procedures. [ABSTRACT FROM AUTHOR]

Published
2017
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4. Anode purge strategy optimization of the polymer electrode membrane fuel cell system under the dead-end anode operation.

Author

Hu, Zhe, Yu, Yi, Wang, Guangjin, Chen, Xuesong, Chen, Pei, Chen, Jun, and Zhou, Su

Subjects
*FUEL cells, *POLYMER electrodes, *MATHEMATICAL optimization, *DIFFUSION, *MASS spectrometry
Abstract

Dead-ended anode (DEA) mode is commonly applied in fuel cell vehicles for the hydrogen purge at the anode side, to reduce fuel waste and enhance fuel cell efficiency. Anode purge is necessary and is definitely important with respect to removing liquid water and accumulated nitrogen in the gas diffusion layer and the flow field of the DEA-mode fuel cell. In this paper, the effect of different purge strategies on the stack performance and system efficiency is investigated experimentally using fast data acquisition and advanced tools, such as the fast cell voltage measurement (CVM) system and the mass spectrum. From the fast data acquisition, the voltage stability, liquid water and nitrogen concentration measurement in the anode exhaust are compared and analyzed under different purge strategy designs and using different purge valves. The results show that under the optimal purge strategy, the DEA fuel cell stack can achieve the desired stability and system efficiency based on the analysis of the cell voltage and purge volume. Moreover, the diameter of the purge valve has a great impact on the voltage stability because a diameter change will result in a different pressure drop and purge volume when the purge valve is open. [ABSTRACT FROM AUTHOR]

Published
2016
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5. Heavy flavor pentaquarks with four heavy quarks

Author

Kan Chen, Xiang Liu, Zhan-Wei Liu, and Hong-Tao An

Subjects
Quark, Physics, Particle physics, 010308 nuclear & particles physics, High Energy Physics::Lattice, High Energy Physics::Phenomenology, FOS: Physical sciences, Interaction model, 01 natural sciences, Pentaquark, High Energy Physics - Phenomenology, High Energy Physics - Phenomenology (hep-ph), 0103 physical sciences, Mass spectrum, High Energy Physics::Experiment, Nuclear Experiment, 010306 general physics, Mass spectrum analysis, Flavor
Abstract

In this work, we carry out the study of heavy flavor pentatuarks with four heavy quarks, which have typical $QQQQ\bar q$ configuration. Within the Chromomagnetic Interaction model, the mass spectrum of these discussed $QQQQ\bar q$ pentaquarks is given. In addition to the mass spectrum analysis, we also illustrate their two-body strong decay behavior by estimating some ratios of decay channels. By these effort, we suggest that future experiment should pay attention to this kind of pentaquark., 16 pages, 2 figures, accepted by Phys. Rev. D

Published
2021

6. GMSimpute: a generalized two-step Lasso approach to impute missing values in label-free mass spectrum analysis

Author

Wenjun Meng, Kate Fisher, Qian Li, Y. Ann Chen, Brooke L. Fridley, Bin Fang, Steven A. Eschrich, Eric A. Welsh, John M. Koomen, and Eric B. Haura

Subjects
Proteomics, Statistics and Probability, Normalization (statistics), Computer science, Two step, computer.software_genre, Biochemistry, Mass Spectrometry, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Bias, Lasso (statistics), Limit of Detection, Cluster Analysis, Metabolomics, Imputation (statistics), Molecular Biology, 030304 developmental biology, Label free, 0303 health sciences, Computational Biology, Missing data, Original Papers, Pearson product-moment correlation coefficient, Computer Science Applications, Random forest, Computational Mathematics, Computational Theory and Mathematics, 030220 oncology & carcinogenesis, symbols, Data mining, Data and Text Mining, Mass spectrum analysis, computer
Abstract

Motivation Missingness in label-free mass spectrometry is inherent to the technology. A computational approach to recover missing values in metabolomics and proteomics datasets is important. Most existing methods are designed under a particular assumption, either missing at random or under the detection limit. If the missing pattern deviates from the assumption, it may lead to biased results. Hence, we investigate the missing patterns in free mass spectrometry data and develop an omnibus approach GMSimpute, to allow effective imputation accommodating different missing patterns. Results Three proteomics datasets and one metabolomics dataset indicate missing values could be a mixture of abundance-dependent and abundance-independent missingness. We assess the performance of GMSimpute using simulated data (with a wide range of 80 missing patterns) and metabolomics data from the Cancer Genome Atlas breast cancer and clear cell renal cell carcinoma studies. Using Pearson correlation and normalized root mean square errors between the true and imputed abundance, we compare its performance to K-nearest neighbors’ type approaches, Random Forest, GSimp, a model-based method implemented in DanteR and minimum values. The results indicate GMSimpute provides higher accuracy in imputation and exhibits stable performance across different missing patterns. In addition, GMSimpute is able to identify the features in downstream differential expression analysis with high accuracy when applied to the Cancer Genome Atlas datasets. Availability and implementation GMSimpute is on CRAN: https://cran.r-project.org/web/packages/GMSimpute/index.html. Supplementary information Supplementary data are available at Bioinformatics online.

Published
2019

7. A Smart Fluorescent Probe Based on Salicylaldehyde Schiff’s Base with AIE and ESIPT Characteristics for the Detections of N2H4 and ClO−

Author

Yujian Tang, Wanying Sun, Jianping Li, Li Bi, Ya Xie, Liqiang Yan, Shaoyang Wang, and Minghui Tang

Subjects
chemistry.chemical_classification, Sociology and Political Science, Base (chemistry), 010405 organic chemistry, Dual emission, Clinical Biochemistry, 010402 general chemistry, Photochemistry, 01 natural sciences, Biochemistry, Fluorescence, 0104 chemical sciences, Clinical Psychology, chemistry.chemical_compound, chemistry, Turn off, Salicylaldehyde, Intramolecular force, Mass spectrum analysis, Law, Spectroscopy, Social Sciences (miscellaneous)
Abstract

Smart and versatile salicylaldehyde Schiff’s bases have been proved their excellent performances including large shocks shift, dual emission wavelengths and sensitive to environment for fluorescence analysis. Herein, a simple salicylaldehyde Schiff’s base molecular (PBAS) with aggregation-induced emission (AIE) and the excited-state intramolecular proton-transfer (ESIPT) effects was constructed for detecting N2H4 and ClO−. The highly specific and sensitive response to N2H4 was witnessed by the fast turn-on of the strong blue fluorescence and to ClO− was observed by the rapid turn off of the weak green fluorescence simultaneous decomposing of the probe. The results of mass spectrum analysis showed that probe PBAS decomposed under the influence of N2H4, whereas probe PBAS can complex with ClO− and prevent effective ESIPT process. Benefiting from its high properties, this fluorescence molecular provides an effective tool for probing N2H4 and ClO− in live cells.

Published
2019

8. Sparse and nonnegative sparse D-MORPH regression.

Author

Li, Genyuan, Rey-de-Castro, Roberto, Xing, Xi, and Rabitz, Herschel

Subjects
*SPARSE graphs, *REGRESSION analysis, *ALGEBRAIC equations, *HOMOTOPY theory, *QUANTUM mechanics, *HIGH-dimensional model representation, *COST functions
Abstract

An underdetermined linear algebraic equation system $$\mathbf{y}={\varvec{\Phi }}\mathbf{x}$$ , where $${\varvec{\Phi }}$$ is an $$m\times n (m

Published
2015
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9. Mass spectra separation for explosives detection by using probabilistic latent component analysis.

Author

Kawaguchi, Yohei, Togami, Masahito, Nagano, Hisashi, Hashimoto, Yuichiro, Sugiyama, Masuyuki, and Takada, Yasuaki

Abstract

We propose a new method to separate mass spectra into components of each chemical compound for explosives detection. In mass spectra, all components have no negative values. However, conventional factor analyses for basis decomposition use no constraints of non-negativity, and we can not apply these methods to mass spectra. The proposed method is based on probabilistic latent component analysis (PLCA). The constraints of non-negativity always hold in PLCA, so that the method is effective for mass spectra. In addition, PLCA is defined in a statistical framework, thus PLCA makes it possible to utilize additional a priori information. Therefore, we introduce sparseness assumptions in the domain of mass spectrometry to PLCA in order to estimate the components more accurately. Experimental results indicate that the proposed method outperforms existing methods. [ABSTRACT FROM PUBLISHER]

Published
2012
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10. Mass spectrum data processing based on compressed sensing recognition and sparse difference recovery.

Author

Liu, Ji-xin and Sun, Quan-sen

Abstract

A new compressed sensing (CS) framework is presented for intelligent mass spectrum data processing in this paper. MS sensing data is used to realize the prior MS analysis through compressed sensing recognition (CSR) method. Then, based on the CSR prior knowledge, we propose the concept of sparse difference (SD) to accomplish high quality CS recovery for high dimensional MS data. The effectiveness and feasibility of the proposed method is validated by numerical experiments. [ABSTRACT FROM PUBLISHER]

Published
2012
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11. Mass Spectrum Analysis of Gas Emitted during Organic Contaminant Removal from a Metal Surface with an Arc in Low Vacuum.

Author

Sugimoto, Masaya and Takeda, Koichi

Subjects
*WASTE gases, *METALLIC surfaces, *POLLUTANTS, *SPECTRUM analysis, *MASS spectrometers, *VACUUM arcs
Abstract

The gas emitted during organic contaminant removal from a metal surface with an arc in low vacuum is investigated using a quadrupole mass spectrometer. The experimental results show that fragment molecules of the contaminant material, which are created by the decomposition of the contaminant material, exist in the emitted gas. The decomposition rate of the contaminant increased with the treatment current, which indicates that the decomposition occurs not in the cathode spot, but in the arc column. © 2006 American Institute of Physics [ABSTRACT FROM AUTHOR]

Published
2006
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12. Monitoring of thermal behavior and decomposition products of soybean oil.

Author

Zhang, Qing, Saleh, Ahmed, Chen, Jing, Sun, Peiran, and Shen, Qun

Subjects
*THERMAL properties, *CHEMICAL decomposition, *SOY oil, *HEAT treatment, *FOURIER transform infrared spectroscopy, *QUADRUPOLE ion trap mass spectrometry
Abstract

The thermal degradation and corresponding decomposition products of fresh and heat-treated soybean oil were investigated by synchronous thermal analyzer combined with Fourier transform infrared spectrometry and quadrupole mass spectrometry (STA-FTIR-QMS). Two longtime heat-treated soybean oil samples were aforehand prepared by consistently heating the fresh soybean oil for 50 and 100 h, respectively. N and simulative air (N/O = 4:1, volume) were used as the thermal reaction gas atmosphere. The results showed that one stage of mass loss appeared in analysis of the all oil samples under N atmosphere condition and longtime heat pre-treatment had no effect on the thermal behavior of the soybean oil under N atmosphere condition. However, four stages occurred in analysis of both untreated and heat-treated oil samples under the simulative air atmosphere condition. Longtime heat pre-treatment influenced the thermal behavior of the soybean oil in certain extent, which was reflected in the different mass loss values of the four stages. According to the infrared absorption profiles and MS spectra of the released compounds in vapor phase, HO, CO, CO, hydrocarbons (such as CH), and hydroxyl, carbonyl, and carboxyl-contained compounds have been confirmed. Therefore, STA-FTIR-QMS can be suggested as a promising technique for investigating of thermal degradation and monitoring the decomposition products of the evolving substances in edible oils. [ABSTRACT FROM AUTHOR]

Published
2014
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13. Possibility of charmoniumlike state X(3915) as χc0(2P) state

Author

Ming-Xiao Duan, Takayuki Matsuki, Si-Qiang Luo, and Xiang Liu

Subjects
Physics, 010308 nuclear & particles physics, 0103 physical sciences, Quark model, Mass spectrum, State (functional analysis), 010306 general physics, Mass spectrum analysis, 01 natural sciences, Mass gap, Mathematical physics
Abstract

In this work, we seriously discuss whether $X(3915)$ can be treated as a ${\ensuremath{\chi}}_{c0}(2P)$ state. Based on an unquenched quark model, we give the mass spectrum of the ${\ensuremath{\chi}}_{cJ}(2P)$ states, where there are no free input parameters in our calculation. Our result shows that the mass gap between ${\ensuremath{\chi}}_{c0}(2P)$ and ${\ensuremath{\chi}}_{c2}(2P)$ can reach 13 MeV, which can reproduce the mass difference between $Z(3930)$ and $X(3915)$. Additionally, the calculated masses of ${\ensuremath{\chi}}_{c0}(2P)$ and ${\ensuremath{\chi}}_{c2}(2P)$ are consistent with experimental values of $X(3915)$ and $Z(3930)$, respectively. Besides, giving the mass spectrum analysis to support $X(3915)$ as ${\ensuremath{\chi}}_{c0}(2P)$, we also calculate the width of ${\ensuremath{\chi}}_{c0}(2P)$ with the same framework, which is also consistent with the experimental data of $X(3915)$. Thus, the possibility of charmoniumlike state $X(3915)$ as ${\ensuremath{\chi}}_{c0}(2P)$ state is further enforced.

Published
2020

14. Analyzing the channel dopant profile in next-generation FinFETs via atom probe tomography

Author

Yong O. Wei, Andrew J. Martin, and Andreas Scholze

Subjects
010302 applied physics, Materials science, Field (physics), Dopant, business.industry, Electron energy loss spectroscopy, 02 engineering and technology, Semiconductor device, Atom probe, 021001 nanoscience & nanotechnology, 01 natural sciences, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, law.invention, law, 0103 physical sciences, Optoelectronics, Diffusion (business), 0210 nano-technology, business, Mass spectrum analysis, Instrumentation, Communication channel
Abstract

Dopant analysis in next-generation semiconductor devices has become increasingly difficult for traditionally used analytical techniques. Atom probe tomography has been viewed by some as a possible solution to these challenges because of its three-dimensional capabilities, forcing the atom probe to mature at a rapid pace in this particular field. This work presents a well-rounded analysis of how APT can be used to examine B dopant diffusion into the channel of a next-generation FinFET, where the channel dimensions and the number of dopants atoms are significantly smaller than any devices measured by APT to date. Complimentary EELS analysis of the gate and channel provides a better understanding of how distortions and artifacts in the APT reconstruction affect the overall integrity of the dataset. Dopant measurements in the channel are confirmed through in-depth mass spectrum analysis and compared with values proposed by TCAD modeling.

Published
2018

16. SNP genotyping for detecting the 'rare allele phenomenon' in hybrid zones.

Author

Lammers, Y., Kremer, D., Brakefield, P. M., Groenenberg, D. S. J., Pirovano, W., and Schilthuizen, M.

Subjects
*SINGLE nucleotide polymorphisms, *ALLELES, *HYBRID zones, *POPULATION genetics, *GENETIC mutation, *SNAILS, *MASS spectrometry, *GASTROPODA, REPRODUCTIVE isolation
Abstract

Hybrid zones are regions where genetically distinct populations meet, mate and produce offspring. In such zones, genetically less compatible gene combinations are usually generated, resulting in reduced fitness, and hybrid zones are often maintained because of continuous removal of unfit genotypes, balanced by gene flow into the zone from the parental populations (and are then referred to as 'tension zones'). Tension zones often display unexpectedly high frequencies of gene variants that are rare outside the zone. Previous work has shown that this 'rare allele phenomenon' is not the result of intragenic recombination or increased mutation rates. Further understanding of the population genetics of the phenomenon requires an approach in which both the numbers of individuals and the numbers of loci is increased. Here, we report an approach using a combination of Illumina next-generation sequencing and mass spectrophotometer genotyping to identify markers that may be used for genome-wide investigations of the rare allele phenomenon. We test this approach on a hybrid zone in the land snail Albinaria hippolyti from Greece. [ABSTRACT FROM AUTHOR]

Published
2013
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17. A proteomic study of Hutchinson–Gilford progeria syndrome: Application of 2D-chromotography in a premature aging disease

Author

Wang, Li, Yang, Wu, Ju, Weina, Wang, Peirong, Zhao, Xinliang, Jenkins, Edmund C., Brown, W. Ted, and Zhong, Nanbert

Subjects
*PROGERIA, *PROTEOMICS, *GENETIC disorders, *CHROMATOGRAPHIC analysis, *GENE expression, *FIBROBLASTS, *FARNESYLTRANSFERASE, *ONTOLOGY
Abstract

Abstract: The Hutchinson–Gilford progeria syndrome (HGPS) is a rare genetic disease characterized by segmental premature aging. Applying a two-dimensional chromatographic proteomic approach, the 2D Protein Fractionation System (PF2D), we identified 30 differentially expressed proteins in cultured HGPS fibroblasts. We categorized them into five groups: methylation, calcium ion binding, cytoskeleton, duplication, and regulation of apoptosis. Among these 30 proteins, 23 were down-regulated, while seven were up-regulated in HGPS fibroblasts as compared to normal fibroblasts. Three differentially expressed cytoskeleton proteins, vimentin, actin, and tubulin, were validated via Western blotting and characterized by immunostaining that revealed densely thickened bundles and irregular structures. Furthermore in the HGPS cells, the cell cycle G1 phase was elongated and the concentration of free cytosolic calcium was increased, suggesting intracellular retention of calcium. The results that we obtained have implications for understanding the aging process. [Copyright &y& Elsevier]

Published
2012
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18. Quantitation of fatty acid ethyl esters in human meconium by an improved liquid chromatography/tandem mass spectrometry

Author

Kwak, Ho-Seok, Kang, Young-Sun, Han, Ki-Ok, Moon, Jong-Taek, Chung, Young-Chul, Choi, June-Seek, Han, Jung-Yeol, Kim, Moon-Young, Velázquez-Armenta, E. Yadira, and Nava-Ocampo, Alejandro A.

Subjects
*FATTY acids, *MECONIUM, *HIGH performance liquid chromatography, *TANDEM mass spectrometry, *ALCOHOL drinking, *NEWBORN infant care
Abstract

Abstract: This paper reports the development and validation of an improved assay for quantitation of fatty acid ethyl esters (FAEEs) in human meconium using liquid chromatography/tandem mass spectrometry (LC–MS/MS). FAAEs (ethyl laurate, ethyl myristate, ethyl palmitate, ethyl palmitoleate, ethyl stearate, ethyl oleate, ethyl linoleate, ethyl linolenate, and ethyl arachidonate) and the internal standard (I.S.), ethyl heptadecanoate, were separated by reverse phase HPLC and quantified by MS/MS using electrospray ionization (ESI) and multiple reaction monitoring (MRM) in the positive ionization mode. The absolute recovery of FAEEs varied from 55±10% for 0.33nmol/g (100ng/g) of ethyl linoleate up to 86±8% for 1.55nmol/g (500ng/g) of ethyl miristate. The LODs and LOQs varied from 0.01 to 0.08nmol/g and from 0.02 to 0.27nmol/g, respectively. The assay has been successfully applied to examine the FAEE levels in 81 meconium samples from babies born to mothers reporting alcohol consumption, to varying degrees, during pregnancy. [Copyright &y& Elsevier]

Published
2010
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19. Phase II metabolites of etofesalamide in filamentous fungi.

Author

Hai-hua Huang, Gui-lei Ma, Yu-ming Sun, Qiang Li, and Da-fang Zhong

Subjects
DRUGS, METABOLITES, FILAMENTOUS fungi, LIQUID chromatography, MASS spectrometry
Abstract

To study phase II metabolites of etofesalamide in filamentous fungi. Seven fungi were screened to transform etofesalamide. The metabolites of etofesalamide were assayed using liquid chromatography coupled to mass spectrometry. The major metabolite was subject to enzymatic hydrolysis to confirm its structure. Etofesalamide was converted into two phase II metabolites: glucoside and riboside conjugates. Glucoside conjugate was the major product with a yield greater than 90%; no phase I metabolites were detected. Glucoside and riboside conjugations of etofesalamide in filamentous fungi differ from the phase II metabolism of glucuronidation in mammals. [ABSTRACT FROM AUTHOR]

Published
2005
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20. Identification of common polymorphisms in the promoter of the UGT1A9 gene.

Author

Girard, Hugo, Court, Michael H, Bernard, Olivier, Fortier, Louis-Charles, Villeneuve, Lyne, Hao, Qin, Greenblatt, David J, Von Moltke, Lisa L, Perussed, Louis, and Guillemette, Chantal

Abstract

Polymorphisms in UDP-glucuronosyltransferases (UGTs) can influence detoxifying capacities and have considerable therapeutic implications in addition to influence various (patho)physiological processes. UGT1A9 plays a central role in the metabolism of various classes of therapeutic drugs in addition to carcinogens and steroids. The great interindividual variability of UGT1A9-mediated glucuronidation remains poorly explained, while evidence for its genetic origin exists.The proximal UGT1A9 promoter was screened for polymorphisms by sequencing and, the contribution of single nucleotide polymorphisms (SNPs) to the variability of UGT1A9 protein levels and activity was evaluated.We confirmed the presence of the -109 to -98 T10 polymorphism and found ten novel SNPs that generated a diversity of haplotypes in two independent populations. In a panel of 48 human liver microsomes, the UGT1A9 expression varied by 17-fold and was significantly correlated with SNPs -275, -331/-440, -665 and -2152. The base insertion T10 reported to increase reporter gene expression in HepG2 cells [33] was not linked to -275 and -2152 SNPs and was not associated with changes in UGT1A9 protein levels. Compared to wild-type individuals, there were statistically significant higher glucuronidating activities in livers with the -275 and -2152 using mycophenolic acid and propofol as UGT1A9 substrates, indicating an extensive glucuronidator phenotype associated with these variants.This is the first study to demonstrate that naturally occurring sequence variations in the UGT1A9 promoter are informative in predicting the levels of protein and glucuronidating activity, providing a potential mechanism for interindividual variation in UGT1A9-mediated metabolism. [ABSTRACT FROM AUTHOR]

Published
2004
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21. Inhibition of cholesterol absorption by phytosterol-replete wheat germ compared with phytosterol-depleted wheat germ.

Author

Ostlund Jr., Richard E., Racette, Susan B., and Stenson, William F.

Abstract

Background: Low-fat vegetable foods contain phytosterols, but it is not known whether they are in biologically active forms or whether their concentrations are high enough to reduce cholesterol absorption and favorably affect lipid metabolism. Objective: The objective was to establish whether the selective removal of phytosterols from wheat germ would increase the cholesterol absorption measured from test meals composed of wheat germ muffins. Design: Wheat germ, which has a high content of phytosterols relative to total fat, was chosen as a low-fat test food. Cholesterol absorption was measured 3 times in 10 subjects. Each test meal was a muffin containing 30 mg heptadeuterated cholesterol tracer and, in random order, 80 g original wheat germ containing 328 mg phytosterols, wheat germ from which phytosterols had been selectively extracted, or extracted wheat germ reconstituted with purified phytosterols. Changes in cholesterol absorption were monitored by the measurement of tracer enrichment of plasma cholesterol 4 and 5 d after each meal with the use of negative ion mass spectrometry. Results: Tracer enrichment of plasma cholesterol was 42.8% higher after consumption of phytosterol-free wheat germ than after that of the original wheat germ (0.415 ± 0.035 compared with 0.291 ± 0.024 μmol tracer/μmol cholesterol; P < 0.01). Tracer enrichment of plasma cholesterol was not significantly different between the wheat germ with extracted-and-reconstituted phytosterol (0.305 ± 0.022 μmol tracer/mmol cholesterol) and the original wheat germ. Conclusion: The efficiency of cholesterol absorption from test meals was substantially lower after consumption of original wheat germ than after consumption of phytosterol-free wheat germ, which suggests that endogenous phytosterols in wheat germ and possibly in other low-fat vegetable foods may have important effects on cholesterol absorption and metabolism that are independent of major nutrients. [ABSTRACT FROM AUTHOR]

Published
2003
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22. Effect of a long-chain n-3 polyunsaturated fatty acid–enriched diet on adipose tissue lipid profiles and gene expression in Holstein dairy cows

Author

Virginie Maillard, Joëlle Dupont, Sandrine Freret, Sébastien Elis, Alice Desmarchais, Juliette Cognie, Valérie Labas, Céline Hivelin, Eric Briant, Svetlana Uzbekova, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Région Centre Val de Loire, INRA, Apis-Gene, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (Unité Expérimentale de Physiologie Animale de l‘Orfrasiére - UE PAO), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, bovin, mass spectrum analysis, tissu adipeux, Adipose tissue, chemistry.chemical_compound, spectrométrie de masse, dairy cows, 2. Zero hunger, chemistry.chemical_classification, Fatty Acids, food and beverages, 04 agricultural and veterinary sciences, Fish oil, adipose tissue, Milk, Docosahexaenoic acid, vache laitière, Female, Polyunsaturated fatty acid, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], medicine.medical_specialty, expression génique, Biology, acide gras polyinsaturé n 3, Fatty acid-binding protein, 03 medical and health sciences, Perilipin-1, Internal medicine, Fatty Acids, Omega-3, Genetics, medicine, Animals, Lactation, Lipolysis, maldi tof, Fatty acid metabolism, 0402 animal and dairy science, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, 040201 dairy & animal science, Diet, 030104 developmental biology, Endocrinology, chemistry, Dietary Supplements, gene expression, Cattle, Animal Science and Zoology, paramètre zootechnique, Food Science
Abstract

The objective of this study was to determine whether fish oil supplement has an effect on adipose tissue lipid profiles and gene expression in postpartum dairy cows. Holstein cows were supplemented with either long-chain n-3 polyunsaturated fatty acid (PUFA; protected fish oil) or control PUFA (n-6; toasted soybeans) for 2mo after calving (n=23 per diet). These cows showed no difference in milk production or metabolic parameters, but exhibited a tendency toward a decrease in early embryo mortality rate after artificial insemination. We hypothesized that, in addition to this effect, modifications in adipose tissue (AT) gene expression and lipid profiles would occur in response to diet. Subcutaneous AT samples were thus collected from the dewlaps of n-3 and n-6 dairy cows at 1mo antepartum, and 1wk, 2mo, and 5mo postpartum for the analysis of lipids and gene expression. Lipid profiles were obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in both positive and negative modes. We found 37 lipid species in the 200 to 1,200 m/z range, which differed between the n-3 and control groups, suggesting that the n-3 supplement affected the lipid composition through the enrichment of lipids integrating long-chain PUFA from fish oil sources: eicosapentaenoic and docosahexaenoic acid. Moreover, a decrease in triacylglycerolipids was observed in AT of n-3 supplemented cows. The expression of 44 genes involved in fatty acid metabolism and the adipokine system was assessed by real-time reverse-transcription PCR. Hierarchical clustering, according to either postpartum stage or diet, enabled us to group genes exhibiting similar kinetic properties during lactation or by those that varied in similar ways after n-3 supplementation, respectively. Among the genes exhibiting a dietary effect, FABP4, LIPE, CD36, and PLIN1 were overexpressed in n-3 AT samples compared with the control, suggesting an increase in lipolysis due to n-3 supplementation, which was reflected on lipolytic activity at the protein level (i.e., protein expression of fatty acid binding protein 4, phosphorylated perilipin 1, and phosphorylated hormone-sensitive lipase). This increase in lipolysis is relevant to the decrease in triglycerides observed in these samples. Gene expression analyses between n-3 and control AT samples also suggested that the n-3 diet could modulate the secretory functions of AT, possibly by affecting adipokine expression; however, this has to be confirmed at the protein level.

Published
2016

23. Linking cocoa polyphenol composition to chocolate quality with Average-Mass-Spectra fingerprints

Author

Veronique Cheynier, Clotilde Hue, Noemie Fayeulle, Nicolas SOMMERER, Jean-Claude Boulet, Emmanuelle Meudec, Renaud Boulanger, Arnaud Verbaere, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Chocolaterie Valrhona, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Valrhona SAS, and Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
analyse sensorielle, polyphénol, spectrométrie de masse, chocolate, mass spectrum analysis, empreinte digitale, [SDV.IDA]Life Sciences [q-bio]/Food engineering, fingerprint, chocolat, sensory analysis
Abstract

Approaches enabling prediction of chocolate quality from cocoa composition would avoid time- and money-consuming steps to chocolate makers. Average mass spectra of cocoa-polyphenol-extracts led to fingerprints used to select the molecules that discriminate chocolate sensory groups.16 worldwide cocoa samples were processed into chocolates which were characterized by sensory analysis, allowing sorting of the samples into four sensory groups.The cocoa polyphenol extracts were analyzed by liquid chromatography−low-resolution mass spectrometry. Averaging each mass spectrum provided polyphenolic fingerprints, which were combined into a matrix and processed with chemometrics (PCA, PLS-DA) to select the most meaningful molecules for discrimination of the chocolate sensory groups.A larger set of 44 cocoa samples was used to validate the previous results. 29 mass signals of known and unknown molecules, mainly flavan-3-ols, were finally targeted[1], including 2 newly described ethyl-bridged flavan-3-ols[2], enabling sensory-group discrimination. Average mass spectra fingerprints of cocoa-polyphenol-extracts proved to be quick and efficient to select the molecules that discriminate chocolate sensory groups.A targeted MRM (Multiple Reaction Monitoring) mass spectrometry method was then developed and validated to routinely analyse large series of cocoa samples.[1] Fayeulle et al., Fast Discrimination of Chocolate Quality Based On Average Mass Spectra Fingerprints of Cocoa Polyphenols, J Agric. Food Chem., 2019, 67, 2723-2731[2] Fayeulle et al., Characterization of new flavan-3-ol derivatives in fermented cocoa beans, Food Chem., 2018 (259), 207-212

Published
2019

24. Digestion of milk proteins: Comparing static and dynamic in vitro digestion systems with in vivo data

Author

Reto Portmann, Didier Dupont, Patrick Schlegel, Desirée Duerr, Lotti Egger, Peter Stoll, Guy Vergères, Olivia Ménard, Christian Baumann, Agroscope, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, COST Action FA1005 INFOGEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
Hydrolyzed protein, food.ingredient, Swine, mass spectrum analysis, Peptide, acide aminé libre, static model, In Vitro Techniques, digestion, human health, High-performance liquid chromatography, Models, Biological, free amino acids, allégations nutritionnelles et de santé, Mass Spectrometry, modèle statique, Hydrolysis, 0404 agricultural biotechnology, food, protéine de lait, In vivo, spectrométrie de masse, Skimmed milk, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Animals, Humans, Chromatography, High Pressure Liquid, Gel electrophoresis, chemistry.chemical_classification, Chromatography, Chemistry, Stomach, 04 agricultural and veterinary sciences, santé humaine, Milk Proteins, 040401 food science, peptide, Gastrointestinal Tract, Intestines, Kinetics, Jejunum, Milk, Digestion, Peptides, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract

Part of special issue: The 5th International Conference on Food Digestion - Edited by Didier Dupont, Pasquale Ferranti, Alan Mackie 5. International Conference on Food Digestion (INFOGEST) ; Rennes (France) - (2017-04-04 - 2017-04-06) / Conférence; In the frame of the COST action INFOGEST, a static in vitro digestion protocol has been elaborated aiming at the improvement of data comparability by harmonizing the experimental conditions. The success in harmonization was confirmed with inter-laboratory trials using skim milk powder as a standardized model food. Moreover, the physiological relevance of the gastric and intestinal endpoints of the static digestion protocol was demonstrated in a pig in vivo trial, with the same skim milk powder and samples collected from different sections of the digestive tract, as well as in a human study with from jejunal effluents. In vivo, digestion is a dynamic process influenced by peristalsis and by the gradual secretion of enzymes and juices and the dwell time of the food. To mimic these physiological mechanisms, dynamic in vitro digestion protocols are widely used. Until now, the differences of protein hydrolysis taking place during dynamic and static in vitro digestion have not been investigated.In this study, the gradual hydrolysis of the main milk proteins present in skim milk powder was digested with the dynamic DIDGI®-system using adult digestion protocol and the static harmonized INFOGEST method. Protein hydrolysis was analyzed by gel electrophoresis, peptide patterns were measured with mass spectrometry, and free amino acids with high pressure liquid chromatography. The peptide patterns at the gastric and intestinal endpoints of in vitro digestion showed a good approximation to the in vivo results from pigs. Moreover, gradual peptide generation was comparable in both in vitro digestion conditions. However, the dynamic protocol reflected the physiological situation better at the level of free amino acid release. Nonetheless, in both in vitro digestion protocols, absorption of free amino acids is not simulated, and they are therefore limited in reflecting the in vivo situation at this level.

Published
2019

25. Fast discrimination of chocolate quality based on average-mass-spectra fingerprints of cocoa polyphenols

Author

Clotilde Hue, Jean-Claude Boulet, Emmanuelle Meudec, Nicolas Sommerer, Noémie Fayeulle, Véronique Cheynier, Renaud Boulanger, Anna Vallverdú-Queralt, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Valrhona SAS, Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Labex Agro: ANR-10- LABX-0001-01, projet 1505-003 Agropolis FOndation/Valrhona, Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1)

Subjects
0106 biological sciences, Polyphénol, Flaveur, cacao, mass spectrum analysis, fingerprint, Chocolat, 01 natural sciences, Matrix (chemical analysis), polyphenolic fingerprint, spectrométrie de masse, Food science, Chocolate, Mathematics, mass spectrometry, food and beverages, Composition chimique, cocoa, General Agricultural and Biological Sciences, Cocoa polyphenols, F60 - Physiologie et biochimie végétale, qualité sensorielle, empreinte digitale, Sensation, Mass spectrometry, Sensory analysis, Chemometrics, Q02 - Traitement et conservation des produits alimentaires, Food Quality, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Theobroma cacao, Q04 - Composition des produits alimentaires, Flavonoids, flavan-3-ols, Plant Extracts, 010401 analytical chemistry, Polyphenols, Reproducibility of Results, General Chemistry, chemometrics, 0104 chemical sciences, Mass spectrum, U30 - Méthodes de recherche, HPLC, 010606 plant biology & botany
Abstract

This work aims to sort cocoa beans according to chocolate sensory quality and phenolic composition. Prior to the study, cocoa samples were processed into chocolate in a standard manner, and then the chocolate was characterized by sensory analysis, allowing sorting of the samples into four sensory groups. Two objectives were set: first to use average mass spectra as quick cocoa-polyphenol-extract fingerprints and second to use those fingerprints and chemometrics to select the molecules that discriminate chocolate sensory groups. Sixteen cocoa polyphenol extracts were analyzed by liquid chromatography-low-resolution mass spectrometry. Averaging each mass spectrum provided polyphenolic fingerprints, which were combined into a matrix and processed with chemometrics to select the most meaningful molecules for discrimination of the chocolate sensory groups. Forty-four additional cocoa samples were used to validate the previous results. The fingerprinting method proved to be quick and efficient, and the chemometrics highlighted 29 m/ z signals of known and unknown molecules, mainly flavan-3-ols, enabling sensory-group discrimination.

Published
2019

26. Static in vitro digestion tests to assess allergenic risk of novel proteins - Use of a peptidomics workflow based on free softwares

Author

Jardin, Julien, Briard-Bion, Valérie, Torcello Gomez, Amélia, Dupont, Didier, Mackie, Alan, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), School of Food Science and Nutrition, University of Leeds, and School of Food Science and Nutrition.

Subjects
mass spectrum analysis, Ingénierie des aliments, allergenicite, allergénicité, protéine végétale, protéine animale, plant protein, allergenicity, spectrométrie de masse, digestion in vitro, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Alimentation et Nutrition, Food and Nutrition, Food engineering, peptidomique, animal protein, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Introduction The resistance of proteins to digestion may play a role in determining their allergenic potential. In order to evaluate the digestibility of proteins, in vitro protocols seem appropriate when ethical constraints hinder in vivo studies. In our study, the ‘pepsin resistance test’, currently used by the European Food Safety Authority to assess allergenicity of proteins is compared to more physiologically relevant in vitro digestion protocols. A peptidomics workflow based on free softwares is used to identify, quantify and perform statistical analysis on the peptides produced. Methods Comparison of an infant, early phase adult, and late phase adult model is being applied on a panel of allergen/non-allergen proteins from animal and vegetal origin. The proteolysis rate in gastric and intestinal compartment is determined in order to detect persistent intact protein (SDS-PAGE) and hydrolysis products larger 9 amino acids (LC-MS). All peptidomics data (NanoLC fitted to Quadrupole-Orbitrap MS) were processed with free softwares: from data conversion to peptide identification, quantification and statistical analysis. Results More than two thousands NanoLC-MS/MS runs were acquired during the course of this project and the number of peptides identified ranged from one to five hundred per proteins depending on their resistance to enzymatic hydrolysis. All peptides identified (X!tandempipeline software [1]) were automatically quantified (MassChroQ [2]) in all samples, regardless the fact that it was identified at this particular time of digestion. Peptide quantification results processed in the R software environment allowed the clustering of peptides based on their behavior regarding the digestion protocol used and the digestion time (0,5 to 60min). Proteins that are not pepsin resistant show slower kinetics of proteolysis for the infant model of digestion, highlighting the fact that, for this population, they may still cause undesired immune responses via sensitization in the duodenum. Conclusions Differences in the kinetics of proteolysis have been found across models for proteins that are not pepsin resistant. This project highlights the importance of a multi-test protocol to assess protein digestibility. Novel Aspect Using a full pipeline of free softwares for peptidomics data treatment, we were able to point out peptide clusters which are only abundant when more physiologically relevant models of digestion were used.

Published
2019

27. The Response of Anesthetic Agent Monitors to Trifluoromethane Warns of the Presence of Carbon Monoxide from Anesthetic Breakdown.

Author

Woehlck, Harvey, Dunning III, Marshall, Kulier, Alexander, Sasse, Frank, Nithipataikom, Kasem, and Henry, Daniel

Abstract

Objective. Trifluoromethane and CO are produced simultaneously duringthe breakdown of isoflurane and desflurane by dry CO2absorbents. Trifluoromethane interferes with anesthetic agent monitoring, andthe interference can be used as a marker to indicate anesthetic breakdown withCO production. This study tests representative types of gas monitors todetermine their ability to provide a clinically useful warning of COproduction in circle breathing systems. Methods. Isoflurane anddesflurane were reacted with dry Baralyme® at 45 °C. Standardizedsamples of breakdown products were created from mixtures of reacted andunreacted gases to simulate the partial degrees of reaction which might resultduring clinical episodes of anesthetic breakdown using 1% or 2% isoflurane and 6% or 12% desflurane. These mixtures were measured by the monitors tested, andthe indication of the wrong agent or a mixture of agents due to the presenceof trifluoromethane was recorded and related to the CO concentration in thegas mixtures. Results. When presented with trifluoromethane fromanesthetic breakdown, monochromatic infrared monitors displayedinappropriately large amounts of isoflurane or desflurane. Agent identifyinginfrared and Raman scattering monitors varied in their sensitivity totrifluoromethane. Mass spectrometers measuring enflurane at mass to charge= 69 were most sensitive to trifluoromethane. Conclusions. Monochromaticinfrared monitors were unable to indicate anesthetic breakdown viainterference by trifluoromethane, but did indicate falsely elevated anestheticconcentrations. Agent identifying infrared and Raman monitors provided warningof desflurane breakdown via the interference of trifluoromethane by displayingthe wrong agent or mixed agents, but may not be sensitive enough to warn ofisoflurane breakdown. Some mass spectrometers provided the most sensitivewarnings to anesthetic breakdown via trifluoromethane, but additional dataprocessing by some patient monitor units reduced their overall effectiveness. [ABSTRACT FROM AUTHOR]

Published
1997
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28. Assigning the newly reported Σb(6097) as a P -wave excited state and predicting its partners

Author

Bing Chen and Xiang Liu

Subjects
Baryon, Physics, Particle physics, 010308 nuclear & particles physics, Excited state, 0103 physical sciences, P wave, Sigma, State (functional analysis), 010306 general physics, Mass spectrum analysis, 01 natural sciences, Omega
Abstract

The newly observed ${\mathrm{\ensuremath{\Sigma}}}_{b}(6097)$ provides us a good chance to further construct the high excited states of the bottom baryon family. In this work, we explain the ${\mathrm{\ensuremath{\Sigma}}}_{b}(6097)$ to be a $1P$ state with ${J}^{P}=5/{2}^{\ensuremath{-}}$ or $3/{2}^{\ensuremath{-}}$ by giving the mass spectrum analysis and the strong decay calculation, and predict the existence of $1P$ and $2S$ partners of the ${\mathrm{\ensuremath{\Sigma}}}_{b}(6097)$, where their masses and the corresponding decay behaviors are presented. Because of the success of describing the ${\mathrm{\ensuremath{\Sigma}}}_{b}(6097)$ and the former observed ${\mathrm{\ensuremath{\Xi}}}_{b}(6227)$ under the quasi-two-body treatment to the bottom baryons, we continue to predict the ${\mathrm{\ensuremath{\Omega}}}_{b}$ partners of ${\mathrm{\ensuremath{\Sigma}}}_{b}(6097)$ by the same method, which are the $1P$ and $2S$ states of the $bss$ baryon system. Identifying these predicted bottom baryons will be a research topic full of challenge and opportunity for future experiments, especially the LHCb.

Published
2018

29. Changes in rumen metabolites from lambs fed a feed additive containing linseed oil and garlic essential oil

Author

Ulli Hohenester, Cristina Saro-Higuera, Marine Gauthier, Stéphanie Durand, Milka Popova, Diego Morgavi, Hamid Boudra, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), ProdInra, Archive Ouverte, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
ecosystem, food additive, additif alimentaire, spectroscopy nuclear magnetic resonance spectroscopy, huile de lin, [SDV]Life Sciences [q-bio], education, mass spectrum analysis, metabolite, agneau, food and beverages, linseed oil, spectroscopie rmn, écosystème, [SDV] Life Sciences [q-bio], garlic, spectrométrie de masse, chromatographie liquide, liquid chromatography, health care economics and organizations, ail
Abstract

Session 2: Microbial ecosystem and herbivore nutritionSession 2: Microbial ecosystem and herbivore nutrition; Changes in rumen metabolites from lambs fed a feed additive containing linseed oil and garlic essential oil. 10. International Symposium on the Nutrition of Herbivores (ISNH10)

Published
2018

30. Role of newly discovered Ξb(6227)− for constructing excited bottom baryon family

Author

Ailin Zhang, Ke-Wei Wei, Xiang Liu, and Bing Chen

Subjects
Baryon, Physics, Particle physics, 010308 nuclear & particles physics, Excited state, 0103 physical sciences, Mass spectrum, State (functional analysis), 010306 general physics, Spectroscopy, Mass spectrum analysis, 01 natural sciences
Abstract

Selecting the newly observed ${\mathrm{\ensuremath{\Xi}}}_{b}(6227{)}^{\ensuremath{-}}$ by LHCb as a study example, we decode its inner structure by giving the mass spectrum analysis and the investigation of its two-body strong decay behaviors. Our result indicates that the ${\mathrm{\ensuremath{\Xi}}}_{b}(6227{)}^{\ensuremath{-}}$ is a good candidate of the $P$-wave ${\mathrm{\ensuremath{\Xi}}}_{b}^{\ensuremath{'}}$ state with ${J}^{P}=3/{2}^{\ensuremath{-}}$ or $5/{2}^{\ensuremath{-}}$. In addition, we further provide the information of the properties of the partners of the ${\mathrm{\ensuremath{\Xi}}}_{b}(6227{)}^{\ensuremath{-}}$. These predicted states include three $2S$ states and the remaining $1P$ states in the bottom-strange baryon family. The calculated sizable Okubo-Zweig-Iizuka--allowed decay widths of these partners show that the experimental search for them becomes possible via LHCb. We have a reason to believe that the present study can be treated as a start point for constructing the highly excited bottom baryon spectroscopy.

Published
2018

32. Casesidin-like anti-bacterial peptides in peptic hydrolysate of camel milk β-casein

Author

Dalila Almi-Sebbane, Emmanuel Bettler, Julien Jardin, Pascal Degraeve, Abderahmane Mati, Nadia Oulahal, Raphaël Terreux, Isabelle Adt, Laboratoire de Biochimie Analytique et Biotechnologies (LABAB), Université Mouloud Mammeri de Tizi Ouzou, Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BIODYMIA), ISARA-LYON (ISARA-LYON)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique UMR 5305 (LBTI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Universit e Mouloud Mammeri de Tizi Ouzou, Isara-Université Claude Bernard Lyon 1 (UCBL), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0301 basic medicine, lait de chamelle, mass spectrum analysis, camel milk, casesidine, medicine.disease_cause, peptide antimicrobien, Applied Microbiology and Biotechnology, Hydrolysate, 03 medical and health sciences, protéine de lait, spectrométrie de masse, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Camel milk, medicine, Food science, Escherichia coli, biology, Molecular mass, Chemistry, 0402 animal and dairy science, 04 agricultural and veterinary sciences, biology.organism_classification, 040201 dairy & animal science, Ultrafiltration (renal), 030104 developmental biology, Staphylococcus aureus, Listeria, peptide antibactérien, Antibacterial activity, beta caséine, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract

The antibacterial activity of camel milk b-casein and its peptic hydrolysate was investigated. The hydrolysatewas fractionated by ultrafiltration successively through membranes with a 10 kDa and a 1 kDacut-off, sequentially. Antibacterial activity assays against Staphylococcus aureus CNRZ 3, Listeria innocuaATCC 33090 and Escherichia coli ATCC 25922 were performed. The growth of S. aureus and L. innocuastrain was not inhibited by b-casein; E. coli strain growth was slightly inhibited. All fractions of hydrolysatesexhibited some anti-bacterial activity and molecular mass fraction

Published
2018

33. Characterizing the Spoilage of Egg Products using Targeted and Non-targeted Approaches

Author

Rémy Coat, Gérald Thouand, Anne Thierry, Olivier Gonçalves, Abdellah Arhaliass, Ali Assaf, Patrick Giraudeau, Estelle Martineau, Frédérique Courant, Jack Legrand, Boris Gouilleux, Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Hydrosciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Mines Nantes (Mines Nantes)-Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS), Deduction modulo, interopérabilité et démonstration automatique (DEDUCTEAM), Laboratoire Spécification et Vérification [Cachan] (LSV), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Bioprocédés Appliqués aux Microalgues (GEPEA-BAM), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Olivier Goncalves, Jack Legrand, Traitement Eau Air Métrologie (GEPEA-TEAM), Matrices Aliments Procédés Propriétés Structure - Sensoriel (GEPEA-MAPS2), and Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
Non targeted, Food industry, ovoproduit, Food spoilage, mass spectrum analysis, industrie agro-alimentaire, Egg product, Raw material, 01 natural sciences, 0404 agricultural biotechnology, contamination, egg product, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, spectrométrie de masse, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, qualité microbiologique, securité alimentaire, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, contamination alimentaire, business.industry, 010401 analytical chemistry, méthode de caractérisation, 04 agricultural and veterinary sciences, Hygienic quality, Pulp and paper industry, 040401 food science, 0104 chemical sciences, Product (business), Whole egg, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, food contamination, oeuf, Environmental science, egg, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

An egg product is a complex matrix, mainly composed of water, proteins and lipids. It also contains carbohydrates, vitamins, minerals and many other compounds in varying concentrations. This chemical complexity makes it difficult to analyze, and most of the protocols are mainly concerned with the albumen and vitellus fractions, aside from the whole egg product. The hygienic quality and freshness of eggs can be assessed according to physico-chemical or microbiological criteria, imposed by the regulations in force. The state provides procedures and recommendations for the production and marketing of such products. Criteria and procedures vary from region to region, for example European legislation forbids the breaking of eggs by centrifugation for eggs intended for the food industry whereas American legislation allows the possibility of breaking eggs by centrifugation if they are clean, disinfected and intact and if they ultimately undergo heat treatment (baking etc.). In addition to the procedures and recommendations concerning the production process, the hygienic quality of egg products is ensured by controlling the physico-chemical and microbiological quality of the raw materials used and the finished products. European legislation thus provides maximum levels for certain compounds such as lactic acid or succinic acid at different points in the production chain.

Published
2018

34. Exploratory GC/MS-Based Metabolomics of Body Fluids

Author

Stéphanie Durand, Carole Migné, Estelle Pujos-Guillot, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Plateforme d’Exploration du Métabolisme, Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Plateforme Exploration du Métabolisme (PFEM), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-MetaboHUB-Clermont, and MetaboHUB-MetaboHUB

Subjects
0301 basic medicine, Chromatography, Silylation, Untargeted metabolomics, Mass spectrometry, Chemistry, gas chromatography, 010401 analytical chemistry, mass spectrum analysis, chromatographie en phase gazeuse, 01 natural sciences, 0104 chemical sciences, Data processing, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Fecal water, Metabolomics, spectrométrie de masse, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Sample preparation, Gas chromatography, Gas chromatography–mass spectrometry, Biomarker discovery, Derivatization
Abstract

Part of the Methods in Molecular Biology book series (MIMB, volume 1730); GC/MS-based metabolomics is a powerful tool for metabolic phenotyping and biomarker discovery from body biofluids. In this chapter, we describe an untargeted metabolomic approach for plasma/serum and fecal water sample profiling. It describes a multistep procedure, from sample preparation, oximation/silylation derivatization, and data acquisition using GC/QToF to data processing consisting in data extraction and identification of metabolites.

Published
2018

35. Methodology for the validation of isotopic analyses by mass spectrometry in stable-isotope labeling experiments

Author

Maud Heuillet, Fabien Létisse, Jean-Charles Portais, Floriant Bellvert, Pierre Millard, Edern Cahoreau, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), National Infrastructure of Metabolomics and Fluxomics, MetaboHUB, Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), MetaToul FluxoMet (TBI-MetaToul), MetaboHUB-MetaToul, MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and MetaToul MetaboHUB, Axiom Platform

Subjects
0301 basic medicine, Accuracy and precision, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, marquage, mass spectrum analysis, High resolution, tagging, Mass spectrometry, Orbitrap, Mass Spectrometry, Analytical Chemistry, law.invention, 03 medical and health sciences, law, Tandem Mass Spectrometry, spectrométrie de masse, Escherichia coli, Metabolomics, Isotopologue, Amino Acids, isotope, Carbon Isotopes, Chemistry, Low resolution, Triple quadrupole mass spectrometer, 030104 developmental biology, réseau métabolique, Isotope Labeling, Stable Isotope Labeling, Biological system
Abstract

Stable-isotope labeling experiments (ILEs) are widely used to investigate the topology and operation of metabolic networks. The quality of isotopic data collected in ILEs is of utmost importance to ensure reliable biological interpretations, but current evaluation approaches are limited due to a lack of suitable reference material and relevant evaluation criteria. In this work, we present a complete methodology to evaluate mass spectrometry (MS) methods used for quantitative isotopic studies of metabolic systems. This methodology, based on a biological sample containing metabolites with controlled labeling patterns, exploits different quality metrics specific to isotopic analyses (accuracy and precision of isotopologue masses, abundances, and mass shifts and isotopic working range). We applied this methodology to evaluate a novel LC-MS method for the analysis of amino acids, which was tested on high resolution (Orbitrap operating in full scan mode) and low resolution (triple quadrupole operating in multiple reaction monitoring mode) mass spectrometers. Results show excellent accuracy and precision over a large working range and revealed matrix-specific as well as mode-specific characteristics. The proposed methodology can identify reliable (and unreliable) isotopic data in an easy and straightforward way and efficiently supports the identification of sources of systematic biases as well as of the main factors that influence the overall accuracy and precision of measurements. This approach is generic and can be used to validate isotopic analyses on different matrices, analytical platforms, labeled elements, or classes of metabolites. It is expected to strengthen the reliability of isotopic measurements and thereby the biological value of ILEs.

Published
2018

36. Evaluation of oxidized phospholipids analysis by LC-MS/MS

Author

Spiro Khoury, Bernard Lyan, Estelle Pujos-Guillot, Corinne Pouyet, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Plateforme d'Exploration du Métabolisme, MetaboHUB, ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Plateforme Exploration du Métabolisme (PFEM), MetaboHUB-Clermont, and MetaboHUB-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)

Subjects
0301 basic medicine, phospholipide, Spectrometry, Mass, Electrospray Ionization, Electrospray ionization, mass spectrum analysis, Autoxidation, Tandem mass spectrometry, Mass spectrometry, 01 natural sciences, Biochemistry, Analytical Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Oxidized phospholipids, Tandem Mass Spectrometry, Fragmentation, spectrométrie de masse, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Phosphatidylcholine, Lipidomics, oxidative stress, Chromatography, High Pressure Liquid, Phospholipids, phospholipid, chemistry.chemical_classification, Phosphatidylethanolamine, Degree of unsaturation, Chromatography, unsaturated fatty acid, 010401 analytical chemistry, Fatty acid, stress oxydatif, acide gras insaturé, maladie métabolique, 0104 chemical sciences, 030104 developmental biology, chemistry, Oxidation-Reduction, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, composé oxyde
Abstract

Phospholipids (PLs) represent a class of metabolites of interest for evaluating the relationship between diet and the development of several metabolic diseases. Given that PLs are rich in unsaturated fatty acids, they can be oxidized. Because of their structure and reactivity, oxidized phospholipids (PLs-Ox) are increasingly recognized as markers of oxidative stress and of various diseases associated with inflammation. Therefore, there is a growing interest in studying PLs-Ox in lipidomics. Because of their limited commercial availability, very little information is currently available in databases to identify these molecules. The aim of this study is to acquire new knowledge about PLs-Ox in order to propose an analytical strategy for their analyses. For this purpose, a synthesis method of PLs-Ox, in auto-oxidation, has been developed and applied on phosphatidylcholine and phosphatidylethanolamine molecular species with various chain lengths, degree, and position of unsaturations. An analysis method based on mass (MS) and tandem mass spectrometry coupled to electrospray ionization was then developed and enabled the identification of a great diversity of long- and short-chain oxidation products. Formation kinetics of oxidation products was evaluated. Results showed that the formation of oxidized compounds was largely influenced by the degree of unsaturation on fatty acid chains. Oxidation time promotes the formation of some biologically important oxidation products. Coupling the MS method with liquid chromatography in flow injection analysis mode enabled the development of a full analytical strategy. Structural analysis of PLs-Ox allowed the enrichment of databases with important information to identify these molecules in biological matrices.

Published
2018

37. Absolute quantification of ppGpp and pppGpp by double-spike isotope dilution ion chromatography–high-resolution mass spectrometry

Author

Clément Patacq, Fabien Létisse, Nicolas Chaudet, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Département de Bioprocédés R&D, Sanofi Pasteur SA, Université Fédérale Toulouse Midi-Pyrénées, Bioprocess R&D department of Sanofi Pasteur, Association Nationale de la Technologique (ANRT), MetaboHUB-ANR-11-INBS-0010, ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), and Université de Toulouse (UT)

Subjects
0301 basic medicine, Spectrometry, Mass, Electrospray Ionization, Absolute quantification, Ion chromatography, mass spectrum analysis, Guanosine, Guanosine Tetraphosphate, Isotope dilution, 01 natural sciences, Analytical Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Isotopes, spectrométrie de masse, Serine, Chromatography, résistance bactérienne, nucléotide, Hydrolysis, 010401 analytical chemistry, thin layer chromatography, Guanosine Pentaphosphate, adaptation au stress, Nucleotidyltransferases, Thin-layer chromatography, 0104 chemical sciences, Kinetics, Double spike, 030104 developmental biology, chemistry, Mutation, bacteria, escherichia coli, Mass spectrum analysis, [CHIM.OTHE]Chemical Sciences/Other, chromatographie en couche mince, Chromatography, Liquid
Abstract

Guanosine 5'-diphosphate 3'-diphosphate (ppGpp) and guanosine S'-triphosphate 3'-diphosphate (pppGpp) play a central role in the adaptation of bacterial and plant cells to nutritional and environmental stresses and in bacterial resistance to antibiotics. These compounds have historically been detected and quantified by two-dimensional thin-layer chromatography of P-32-radiolabeled nucleotides. We report a new method to quantify ppGpp and pppGpp in complex biochemical matrix using ion chromatography coupled to high-resolution mass spectrometry. The method is based on isotopic dilution mass spectrometry (IDMS) using C-13 to accurately quantify the nucleotides. However, the loss of a phosphate group from pppGpp during the sample preparation process results in the erroneous quantification of ppGpp. This bias was corrected by adding an extra N-15 isotope dilution dimension. This double-spike IDMS method was applied to quantify the ppGpp and pppGpp in Escherichia coli and in a mutant strain deleted for gppA (encoding the ppGpp phosphohydrolase) before and after exposure of both strains to serine hydroxamate, known to trigger the accumulation of these nucleotides.

Published
2018

38. New flavanol O-glycosides in grape and wine

Author

Alexander Lepak, Véronique Cheynier, Nancy Terrier, Cédric Saucier, Bernd Nidetzky, Jean Paul Mazauric, Marie Zerbib, Emmanuelle Meudec, Christine Le Guernevé, Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), University of Montpellier (Bourse ecole doctorale GAIA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
0106 biological sciences, Spectrometry, Mass, Electrospray Ionization, Magnetic Resonance Spectroscopy, mass spectrum analysis, Molecular Conformation, Food chemistry, Mass spectrometry, 01 natural sciences, Catechin, Analytical Chemistry, chemistry.chemical_compound, polyphénol, spectrométrie de masse, vin, Vitis, Glycosides, wine, Chromatography, High Pressure Liquid, mass spectrometry, Flavonoids, chemistry.chemical_classification, Wine, flavan-3-ol glycoside, Chromatography, Plant Extracts, 010401 analytical chemistry, Glycoside, General Medicine, grape, 0104 chemical sciences, Monomer, chemistry, oenologie, Polyphenol, Seeds, Dimerization, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Procyanidin dimer, 010606 plant biology & botany, Food Science
Abstract

The presence of monomeric and dimeric flavan-3-ol monohexosides was investigated in grapes and wines. Polyphenol extracts were prepared from grape seeds and skins (Syrah, Merlot, and Cabernet-Sauvignon) sampled at three different developmental stages. Different wines (Tannat, Alicante, Syrah, Merlot, and Grenache) were also studied. The different samples obtained were analyzed by UPLC-ESI-IT-MS. Specific molecular ions corresponding to flavan-3-ol hexosides were detected by using targeted molecular ions with specific m/z values: 451 for (epi) catechin hexosides, and 739 for procyanidin dimer hexosides. 4'-O-beta-glucosyl-(+)-catechin and 7-O-beta-glucosyl-(+)-catechin were obtained by using a glucosyl transferase from apple, UGT71A15, and their structures determined by NMR. These glucosylated monomers and the dimers were identified in all analyzed grape seed and several skin extracts at the different stages and in wines made from different varieties.

Published
2018

39. Metabolomic study of the response to cold shock in a strain of Pseudomonas syringae isolated from cloud water

Author

Cédric Mendes, Isabelle Canet, Martine Sancelme, Pierre Amato, Anne-Marie Delort, Cyril Jousse, Marie Lagree, Mounir Traïkia, Céline Dalle, Bernard Lyan, Institut de Chimie de Clermont-Ferrand (ICCF), SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Plateforme d'Exploration du Métabolisme, MetaboHUB, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Plateforme d’exploration du métabolisme, Institut National de la Recherche Agronomique (INRA), Plateforme Exploration du Métabolisme (PFEM), MetaboHUB-Clermont, MetaboHUB-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Institut de Chimie de Clermont-Ferrand - Clermont Auvergne (ICCF), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Unité de Nutrition Humaine - Clermont Auvergne (UNH), and Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA)

Subjects
0301 basic medicine, Magnetic Resonance Spectroscopy, 010504 meteorology & atmospheric sciences, Endocrinology, Diabetes and Metabolism, influence de la température, Clinical Biochemistry, mass spectrum analysis, Pseudomonas syringae, 01 natural sciences, Biochemistry, Antioxidants, Mass Spectrometry, chemistry.chemical_compound, Cryoprotective Agents, spectrométrie de masse, oxidative stress, cloud, activité microbienne, bactérie, biology, Strain (chemistry), Pseudomonas, ROS, bacterium, Adaptation, Physiological, peptide, Cold Temperature, eau de nuage, Water Microbiology, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, microbial activity, analyse métabolomique, 03 medical and health sciences, Alkaloids, Metabolomics, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Metabolome, 0105 earth and related environmental sciences, Cold-Shock Response, stress oxydatif, Metabolism, MS, biology.organism_classification, Trehalose, [SDE.ES]Environmental Sciences/Environmental and Society, NMR, 030104 developmental biology, chemistry, peptides, cold stress, Database Management Systems, Energy Metabolism, Bacteria
Abstract

This research has been supported by the ANR French program MetaboHUB. We would like to thank Mélanie Pétéra for her valuable technical assistance; AbstractIntroduction: Active microorganisms have been recently discovered in clouds, thus demonstrating the capacity of microorganisms to exist in harsh environments, including exposure to UV and oxidants, osmotic and cold shocks, etc. It is important to understand how microorganisms respond to and survive such stresses at the metabolic level.Objectives: The objective of this work is to assess metabolome modulation in a strain of Pseudomonas syringae isolated from cloud water and facing temperature downshift from 17 to 5 °C by identifying key molecules and pathways of the response/adaptation to cold shock.Methods: Bacterial extracts from suspensions of cells grown at 17 °C and further incubated in microcosms at 5 and 17 °C to mimic cloud conditions were analysed by combining LC-MS and NMR; the results were evaluated in comparison to similar suspensions kept at constant temperature. The differences in the metabolome profiles were deciphered using multivariate statistics (PLS-DA).Results: Key cold shock biomarkers were observed, including cryoprotectants (trehalose, glucose, glycerol, carnitine, glutamate), antioxidants (glutathione and carnitine) and their precursors, alkaloids (bellendine and slaframine) and metabolites involved in energy metabolism (ATP, carbohydrates). Furthermore, new short peptides (nine dipeptides and a tetrapeptide) were found that have no known function.Conclusions: This study shows that in response to cold temperatures, Pseudomonas syringae PDD-32b-74 demonstrates numerous metabolism modifications to counteract the impacts of low temperatures.

Published
2018

40. Strain effect on extracellular laccase activities from Botrytis cinerea

Author

Quijada Morin, Natalia, Garcia, François, Lambert, Karen, Walker, Anne-Sophie, Tiers, Laurent, Viaud, Muriel, Sauvage, Francois Xavier, Hirtz, Christophe, Saucier, Cédric, Sciences Pour l'Oenologie (SPO), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), BIOlogie et GEstion des Risques en agriculture (BIOGER), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Biolaffort Company, Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
moissisure de la grappe, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Laccase-3-BcLCC7, enzyme, Laccase-2-BcLCC2, spectrométrie de masse, mass spectrum analysis, botrytis cinerea, peptide, séquençage de gènes, laccase
Abstract

Background and Aims: Laccase enzymes produced by Botrytis cinerea are involved in the oxidation of phenolic substances during the development of grey mould, which causes significant economic losses. The aim of this work was to study the structural and activity characteristics of the laccase enzymes secreted by three B. cinerea strains that are involved in the development of grey mould. Methods and Results: Laccase enzymes obtained from three B. cinerea strains [one reference strain (B05.10) and two strains obtained from two French vineyards (VA612 and RM344)] were characterised. Analysis by LC-QTOF-MS revealed that the three strains contained a mixture of Laccase-2-BcLCC2 and Laccase-3-BcLCC7. The structural characteristics of the laccases from the three strains, such as molecular weight and glycosylation degree, were identical. Nevertheless, their catalytic activities were significantly different. Conclusions: Differences in catalytic activities could be due either to possible differences in the relative amount of Laccase-2- BcLCC2 and Laccase-3-BcLCC7 produced by each strain or to differences in the glycosidic fraction of the enzymes. Significance of the Study: The severity of the infection caused by B. cinerea may be not only related to the infection level but also to the strain involved.

Published
2018

41. Characterization of new flavan-3-ol derivatives in fermented cocoa beans

Author

Véronique Cheynier, Anna Vallverdú-Queralt, Emmanuelle Meudec, Noémie Fayeulle, Clotilde Hue, Renaud Boulanger, Nicolas Sommerer, Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Chocolaterie Valrhona, Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Agropolis fondation ID ID 1505-003, LabEx Agro ANR-10-LABX-0001-01, Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Agropolis fondation ID ID 1505-003, ANR-10-LABX-0001,AGRO,Agricultural Sciences for sustainable Development(2010), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
Flaveur, cacao, mass spectrum analysis, 01 natural sciences, Catechin, Analytical Chemistry, chemistry.chemical_compound, Bioreactors, spectrométrie de masse, Food science, Microbial metabolite, Chromatography, High Pressure Liquid, mass spectrometry, chemistry.chemical_classification, ethyl bridged flavan-3-ols, food and beverages, 04 agricultural and veterinary sciences, General Medicine, Composition chimique, 040401 food science, cocoa, Mass spectrum analysis, Dimerization, Procyanidin dimer, Spectrometry, Mass, Electrospray Ionization, cocoa beans, épicatéchine, Flavan-3-ol, Mass spectrometry, 0404 agricultural biotechnology, Isomerism, Biflavonoids, Proanthocyanidins, Theobroma cacao, Q04 - Composition des produits alimentaires, polyphenols, Flavonoids, flavan-3-ols, 010401 analytical chemistry, Acetaldehyde, 0104 chemical sciences, chemistry, Polyphenol, Fermentation, Composé de la flaveur, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract

Two series of compounds showing mass signals at m/z 605 and 893 (negative ionization mode) have been detected in fermented cocoa beans. This study objective is to identify these mass signals and characterize their structure in fermented cocoa samples. Our hypothesis is that these signals may correspond to ethyl-bridged flavan-3-ols resulting from flavan-3-ol condensation with acetaldehyde which is a microbial metabolite. Mass spectrometry was used to compare the retention times and mass fragmentation patterns between a model solution using epicatechin and procyanidin dimer B2, the major flavan-3-ols of cocoa, as precursors and extracts of fermented cocoa. Their identification was confirmed: four isomers of ethyl-linked epicatechin as well as several isomers of epicatechin-ethyl-procyanidin B2, in which B2 was mostly linked through its upper unit, were characterized in cocoa. This study demonstrates the presence of flavan-3-ol acetaldehyde condensation products in fermented cocoa beans and provides the first report of epicatechin-ethyl-procyanidin B2.

Published
2018

42. Bacillus thuringiensis produces the lipopeptide thumolycin to antagonize microbes and nematodes

Author

Ming Sun, Ya-Jie Tang, Lifang Ruan, Bingbing Xue, Yaoyao Deng, Dehong Zheng, and Zhiyong Zeng

Subjects
0301 basic medicine, Insecticides, Nematoda, 030106 microbiology, Bacillus thuringiensis, Microbial Sensitivity Tests, Biology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Lipopeptides, Biosynthesis, Anti-Infective Agents, Bacterial Proteins, Gene cluster, Animals, Caenorhabditis elegans, Homologous Recombination, Pest Control, Biological, Molecular mass, Bacteria, Antinematodal Agents, Lipopeptide, Antimicrobial, biology.organism_classification, Reverse genetics, Molecular Weight, chemistry, Biochemistry, Biological Control Agents, Genes, Bacterial, Multigene Family, Mass spectrum analysis, Gene Deletion
Abstract

Bacillus thuringiensis has been widely used as a bio-insecticide. However, novel biological activities other than insect toxicity of B. thuringiensis are still underestimated. In this study, a new lipopeptide biosynthesis gene cluster in B. thuringiensis BMB171 was discovered by genome mining and verified by reverse genetics. Thumolycin, the lipopeptide synthesized by this gene cluster, was then isolated and purified. Mass spectrum analysis revealed the molecular mass of thumolycin is 696.51 Da with the predicted molecular formula of C38H64N8O4. Further bioactivities assay showed that thumolycin endowed B. thuringiensis BMB171 with broad spectrum antimicrobial and nematocidal activities.

Published
2017

43. Systems metabolomics for prediction of metabolic syndrome

Author

Eléonore Herquelot, Mélanie Pétéra, Bernard Lyan, Charlotte Joly, Marcel Goldberg, Dhouha Grissa, Marion Brandolini, Blandine Comte, Marie Zins, Sébastien Czernichow, Estelle Pujos-Guillot, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Plateforme d'Exploration du Métabolisme, Institut National de la Recherche Agronomique (INRA), UMS 011, Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Plateforme Exploration du Métabolisme (PFEM), MetaboHUB-Clermont, MetaboHUB-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Cohortes épidémiologiques en population (CONSTANCES), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Université Paris Cité (UPCité), Département de Nutrition, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université Paris Descartes - Paris 5 (UPD5), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), UMR 1168, ANR-INBS-0010, INRA DID'IT Metaprogramme, MetaboHUB, UMS011 Cohortes épidémiologiques en population (CONSTANCES), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Université de Paris (UP), ProdInra, Archive Ouverte, Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Centre de Recherche en Nutrition Humaine d'Auvergne ( CRNH d'Auvergne ), UMS 011 - Cohortes épidémiologiques en population, Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Régional Universitaire [Lille] ( CHRU Lille ), Université Paris Descartes - Paris 5 ( UPD5 ), Université de Versailles Saint-Quentin-en-Yvelines ( UVSQ ), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-MetaboHUB-Clermont, MetaboHUB-MetaboHUB, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)

Subjects
0301 basic medicine, Male, approche métabolomique, [SDV]Life Sciences [q-bio], mass spectrum analysis, Disease, Bioinformatics, Biochemistry, Human health, systems approach, spectrométrie de masse, database, syndrome métabolique, Systems Biology, metabolic x syndrome, cohort, [ SDV.MHEP.EM ] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Middle Aged, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, metabolomics, 3. Good health, [SDV] Life Sciences [q-bio], Phenotype, Disease Progression, Biomarker (medicine), biomarker, France, biomarqueur, Metabolic profile, Validation study, Food habits, Computational biology, Biology, metabolic syndrome, analyse métabolomique, 03 medical and health sciences, Metabolomics, Predictive Value of Tests, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, cohorte, medicine, Humans, General Chemistry, medicine.disease, 030104 developmental biology, Case-Control Studies, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry, Metabolic syndrome, Biomarkers, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

We thank Yunfei Liu for his contribution concerning correlation network analyses. All metabolomics analyses were performed within the metaboHUB French infrastructure (ANR-INBS-0010). This study was supported by the INRA DID'IT Metaprogramme. The GAZEL cohort received approvals from the National Commission for Data Processing and Freedoms (CNIL), the National Medical Council and the National Consultative Committee of Ethics, and the INSERM IRB. The study protocol was approved by the GAZEL Scientific Committee. All volunteers gave written and informed consent for this study. All the experiments conformed to the principles set out in the WMA Declaration of Helsinki and the Department of Health and Human Services Belmont Report; The evolution of human health is a continuum of transitions, involving multifaceted processes at multiple levels, and there is an urgent need for integrative biomarkers that can characterize and predict progression toward disease development. The objective of this work was to perform a systems metabolomics approach to predict metabolic syndrome (MetS) development. A case-control design was used within the French occupational GAZEL cohort (n =112 males: discovery study n = 94: replication/validation study). Our integrative strategy was to combine untargeted metabolomics with clinical, sociodemographic, and food habit parameters to describe early phenotypes and build multidimensional predictive models. Different models were built from the discriminant variables, and prediction performances were optimized either when reducing the number of metabolites used or when keeping the associated signature. We illustrated that a selected reduced metabolic profile was able to reveal subtle phenotypic differences 5 years before MetS occurrence. Moreover, resulting metabolomic markers, when combined with clinical characteristics, allowed improving the disease development prediction. The validation study showed that this predictive performance was specific to the MetS component. This work also demonstrates the interest of such an approach to discover subphenotypes that will need further characterization to be able to shift to molecular reclassification and targeting of MetS

Published
2017

44. Development of a Mass Spectrometry Imaging (MSI) approach to explore Listeria monocytogenes biofilms exposed to a dehumidification stress

Author

Santos, Tiago, Theron, Laetitia, Chambon, Christophe, Viala, Didier, Centeno, Delphine, Hébraud, Michel, Microbiologie Environnement Digestif Santé (MEDIS), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), Université Clermont Auvergne (UCA)-INRA Clermont-Ferrand-Theix, Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne (UCA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
microorganisme, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, spectrométrie de masse, Microbiology and Parasitology, mass spectrum analysis, déshumidification, biochemical phenomena, metabolism, and nutrition, listeria monocytogènes, Microbiologie et Parasitologie, digestive system diseases, biofilm, dehumidification
Abstract

Development of a Mass Spectrometry Imaging (MSI) approach to explore Listeria monocytogenes biofilms exposed to a dehumidification stress. 8. Colloque du Réseau National Biofilms

Published
2017

45. Signatures métabolomiques par spectrométrie de masse et risque de cancer du sein

Author

Aicha Demidem, Mathilde Touvier, Emmanuelle Kesse-Guyot, Lucie Lécuyer, Pilar Galan, Bernard Lyan, Thomas Ferreira, Claudine Manach, Marie Lagree, Paule Latino-Martel, Valentin Partula, Céline Dalle, Mélanie Deschasaux, Stéphanie Durand, Bernard Srour, Estelle Pujos-Guillot, Marie-Paule Vasson, Mélanie Pétéra, S. Hercberg, Adrien Rossary, Delphine Centeno, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre de Recherche en Nutrition Humaine, Département de Santé Publique, Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Clermont-Ferrand, Fondation ARC., Réseau National Alimentation Cancer Recherche (réseau NACRe). FRA., ProdInra, Archive Ouverte, département de santé publique, Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Societe Francaise de Nutrition Enterale et Parenterale (SFNEP). et Societe Francaise de Nutrition (SFN), FRA., Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Hôpital Avicenne, Assistance Publique - Hôpitaux de Paris (AP-HP), Centre Jean Perrin - Centre Régional de Lutte contre le Cancer d'Auvergne, and Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)

Subjects
0303 health sciences, Nutrition and Dietetics, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, 030309 nutrition & dietetics, Endocrinology, Diabetes and Metabolism, cancer du sein, mass spectrum analysis, étude prospective, 030209 endocrinology & metabolism, [SDV.CAN]Life Sciences [q-bio]/Cancer, mammary malignant tumor, 3. Good health, 03 medical and health sciences, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, 0302 clinical medicine, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, spectrométrie de masse, Internal Medicine, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, métabolomique, plasma
Abstract

Remerciements : Institut National du Cancer (INCa) / Institut Fédératif de Recherche biomédicale (IFRB) / Canceropôle Ilede- France / Réseau NACReRemerciements : Institut National du Cancer (INCa) / Institut Fédératif de Recherche biomédicale (IFRB) / Canceropôle Ilede-France / Réseau NACRe; Introduction et but de l’étude : La métabolomique permet d’étudier l’ensemble des métabolites présents dans unbiofluide. Cette étude basée sur une analyse non ciblée en spectrométrie de masse a pour but d’étudier si des profils métabolomiques établis à partir d’un prélèvement sanguin sur des femmes à priori en bonne santé, pourrait contribuer à prédire le risque à long terme de développer le cancer du sein et d’améliorer la compréhension de son étiologie.Matériel et méthodes : Un cas-témoin niché prospectif a été mené dans la cohorte SU.VI.MAX, incluant 211 cas de cancer du sein et 211 témoins appariés. Les profils métabolomiques ont été établis sur des échantillons de plasma prélevés à l’inclusion, avant l’apparition du cancer, grâce à une analyse non ciblée par spectrométrie de masse (LC-MS). Des modèles de régressions logistiques conditionnelles multivariées ont été calculés sur chaque ion présélectionné grâce à une ANOVA et sur les combinaisons de potentiels biomarqueurs issus d’une Analyse en Composante Principale. Une correction de test multiple de type FDR (False Discovery Rate) a été appliquée.Résultats et Analyse statistique : Plusieurs métabolites étaient associés au risque de cancer du sein. La finalisation de l’identification des métabolites discriminants est en cours. Au vu des résultats actuels, les femmes caractérisées par un niveau plasmatique plus élevé de valine (OR=1,45 [1,15-1,83]; p= 0.009), de phénylalanine (OR=1,43 [1,14-1,78]; p=0,009), de tryptophane (OR=1,4 [1,1-1,79]; p=0,01), et de glutamine (OR=1,33 [1,07-1,66]; p=0,01) et d’un niveau plus bas d’OSuccinyl-L-homoserine (OR=0,7 [0,55-0,89]; p=0,01) auraient un risque plus important de développer le cancer du sein dans la décennie qui suit.Conclusion : Cette étude suggère plusieurs associations entre profils métabolomiques à l’inclusion et risque à long terme de développer le cancer du sein et confirme certains résultats que nous avons mis en évidence par une analyse RMN. Cette étude pourrait aider à améliorer l’identification des femmes à risque de développer un cancer du sein, bien avant l’apparition des symptômes. L’étude des biomarqueurs nutritionnels associés au risque de développer cette pathologie (actuellement en cours) pourra permettre de préciser le rôle de la nutrition (facteur modifiable) dans l’étiologie du cancer du sein et d’améliorer les stratégies préventives.

Published
2017

46. Highly Selective and Sensitive Detection of Hg(II) from HgCl2 by a Simple Rhodamine-Based Fluorescent Sensor

Author

Yan Mao, Aifeng Liu, Hong Miaomiao, and Dongmei Xu

Subjects
Sociology and Political Science, Metal ions in aqueous solution, Clinical Biochemistry, Analytical chemistry, Biochemistry, Rhodamine, chemistry.chemical_compound, Limit of Detection, Spectroscopy, Fluorescent Dyes, Detection limit, Rhodamines, Chemistry, Mercury, Hydrogen-Ion Concentration, Highly selective, Fluorescence, Clinical Psychology, Fluorescence intensity, Hydrazines, Spectrometry, Fluorescence, Metals, Mercuric Chloride, Rhodamine B hydrazide, Mass spectrum analysis, Law, Social Sciences (miscellaneous)
Abstract

N-acryloyl rhodamine B hydrazide, a non-responsive control of a colorimetric Cu(2+) sensor, was used as a turn-on fluorescent sensor for Hg(II) from HgCl2 in the presence of AgNO3. The detection was highly selective and sensitive, and a large number of environmentally and biologically relevant metal ions, such as Na(+), K(+), Ca(2+), Mg(2+), Fe(3+), Cu(2+), Zn(2+), Cr(3+), Pb(2+), Ni(2+), Fe(2+), Mn(2+), Co(2+), Cd(2+), including Hg(II) from the easy dissociated salts, did not show significant interference. The fluorescence of the sensor (10 μM) was enhanced 74 folds by 10 equiv. of Hg(II) from HgCl2 in THF/HAc-NaAc (1/1, v/v, pH = 6) aqueous buffer solution containing 20 equiv. of AgNO3. The maximal fluorescence intensity increased linearly with the concentration of Hg(II) in the range of 0-70 μM. The detection limit of Hg(II) was 0.59 μM. The sensing mechanism was explored by Job's plot experiment, reversible experiment, mass spectrum analysis, spectroscopic analysis, and thin-layer chromatography.

Published
2015

47. Distinct photoproducts of hydroxylated polybromodiphenyl ethers from different photodegradation pathways: a case study of 2′-HO-BDE-68

Author

Hong-Bin Xie, Hongxia Zhao, Qing Xie, Jingwen Chen, and Xingbao Wang

Subjects
Photolysis, Photodissociation, Kinetics, Public Health, Environmental and Occupational Health, Ether, General Medicine, Management, Monitoring, Policy and Law, Hydroxylation, Photochemistry, chemistry.chemical_compound, Models, Chemical, chemistry, Halogenated Diphenyl Ethers, Environmental Chemistry, Photodegradation, Mass spectrum analysis, Bond cleavage
Abstract

Hydroxylated polyhalodiphenyl ethers (HO-PXDEs) are emerging aquatic pollutants. Previous studies have shown that HO-PXDEs can photogenerate dioxins and phenolic compounds. However, it is unclear which photochemical pathways are responsible for the various photoproducts. This study investigates the direct photolysis and photooxidation initiated by (1)O2 and ˙OH that can be formed by photosensitization, taking 2'-HO-2,3',4,5'-tetrabromodiphenyl ether (2'-HO-BDE-68) as a case study. The results show that 1,3,8-tribromodibenzo-p-dioxin can only be produced during direct photolysis. By mass spectrum analysis, four dihydroxylated polybromodiphenyl ethers, generated from both direct and indirect photodegradation were confirmed. Among them, di-HO-tribromodiphenyl ether (di-HO-TBDE) was the main product generated from direct photohydrolysis. Most probably, the di-HO-TBDE is 2',5'-HO-2,3',4-tribromodiphenyl ether, as was suggested by density functional theory calculations. Ether bond cleavage is a dominant pathway for the direct photolysis and photooxidation reactions leading to 2,4-dibromophenol as the dominant product. The yields of the products, which are irrespective of reaction time and can be employed to compare the ability of different HO-PXDEs to photogenerate a given product, were reported. This study indicates that for accurate ecological risk assessment of HO-PXDEs, their different photodegradation pathways that may lead to different photoproducts should be considered.

Published
2015

48. MALDI imaging and profiling mass spectrometry approach for the analysis of Listeria monocytogenes biofilms exposed to a desiccation

Author

Santos, Tiago, Theron, Laetitia, Chambon, Christophe, Viala, Didier, Centeno, Delphine, Hébraud, Michel, Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), Université Clermont Auvergne (UCA)-INRA Clermont-Ferrand-Theix, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Microbiologie Environnement Digestif Santé (MEDIS), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, spectrométrie de masse, Microbiology and Parasitology, mass spectrum analysis, listeria monocytogènes, Microbiologie et Parasitologie, biofilm, matrix assisted laser desorption ionization, maldi
Abstract

MALDI imaging and profiling mass spectrometry approach for the analysis of Listeria monocytogenes biofilms exposed to a desiccation. SMMAP 2017, Spectrométrie de Masse, Métabolomique et Analyse Protéomique

Published
2017

49. Comparison of lipid profiles and gene expression in granulosa and cumulus cells in bovine

Author

Bertevello, Priscila, Banliat, Charles, Teixeira-Mechin, Ana-Paula, Elis, Sébastien, Maillard, Virginie, Carvalho, Anais, Duranthon, Véronique, Labas, Valérie, Uzbekova, Svetlana, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), University do Est ado do Rio Grande do Norte (Brazil), Association Européenne de Transfert Embryonnaire - European Embryo Transfer Association (AETE). FRA., Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
endocrine system, cellule de granulosa, métabolisme lipidique, bovin, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, expression génique, urogenital system, mass spectrum analysis, cellule du cumulus, cumulus, granulosa, lipid metabolism, Biotechnologies, spectrométrie de masse, gene expression
Abstract

Comparison of lipid profiles and gene expression in granulosa and cumulus cells in bovine. 33. Annual Meeting of the European Embryo Transfer Association (AETE)

Published
2017

50. Development of a MALDI imaging mass spectrometry (IMS) approach to bacterial proteomics: first application to Listeria monocytogenes biofilms exposed to a desiccation

Author

Santos, Tiago, Theron, Laetitia, Centeno, Delphine, Chambon, Christophe, Viala, Didier, Hébraud, Michel, Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne (UCA), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Microbiologie Environnement Digestif Santé (MEDIS), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Université Clermont Auvergne (UCA)-INRA Clermont-Ferrand-Theix

Subjects
proteomics, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, spectrométrie de masse, Microbiology and Parasitology, mass spectrum analysis, protéomique, listeria monocytogènes, Microbiologie et Parasitologie, biofilm
Abstract

Development of a MALDI imaging mass spectrometry (IMS) approach to bacterial proteomics: first application to Listeria monocytogenes biofilms exposed to a desiccation. 7. Congress of European Microbiologists FEMS 2017

Published
2017

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