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5 results on '"milieu de conservation"'

1. Avulsions traumatiques des dents permanentes.

Author

SKHIRI, S. ZOUITEN, ABDELMOUMEN, E., JEMAA, M., DOUKI, N., OUESLATI, A., ZOKKAR, N., CHAFRA, N., and BOUGZALLAH, A.

Abstract

Traumatic dental avulsion is an emergency. In permanent dentures, it more often requires replantation. Today's conditions enabling successful replantation treatment are well known, and include mainly preservation and extra-alveolar time of the avulsed tooth and replantation and splint techniques. The aim of this article is to review the current literature data concerning the treatment of avulsed permanent teeth, the management of complications associated with this trauma, and the means to prevent or limit sequelae. [ABSTRACT FROM AUTHOR]

Published
2013
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2. Optimisation et mise en oeuvre d'une technique de cryopréservation du sperme de lignées transgéniques de médaka (Oryzias latipes) et fécondation in vitro

Author

Pagneux, Cyriane, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Lorraine, IUT Nancy-Brabois, and ProdInra, Archive Ouverte

Subjects
fish, fécondation, medaka, cryoprotective agent, cryopréservation, cryoprotecteur, fécondation in vitro, Oryzias latipes, cryoconservation du sperme, [SDV]Life Sciences [q-bio], sperm retrieval, stripping, cryoconservation, collecte de sperme, technique de congelation, reproduction, [SDV] Life Sciences [q-bio], milieu de conservation, dissection, poisson, lignée transgénique, adrianichthyidae, in vitro fertilization, congelation du sperme, motilité du sperme
Abstract

Optimisation et mise en oeuvre d'une technique de cryopréservation du sperme de lignées transgéniques de Médaka (Oryzias latipes) et fécondation in vitro

Published
2017

3. Differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not

Author

François-Xavier Martin, Marianne Vidament, Michèle Magistrini, Elisabeth Blesbois, Pierrick Vincent, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Haras Nationaux, Parc Régional Naturel du Marais Poitevin, Partenaires INRAE, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
sperme, reproduction animale, Cryopreservation, MILIEU DE CONSERVATION, 0403 veterinary science, chemistry.chemical_compound, Cryoprotective Agents, Endocrinology, Food Animals, Pregnancy, Insemination, Artificial, ETHYLENE GLYCOL, 04 agricultural and veterinary sciences, General Medicine, Science des productions animales, ANE, insémination artificielle, Semen cryopreservation, spermatozoide, jument, Cold Temperature, [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies, Sperm Motility, sperme congelé, Female, Donkey, Animal production studies, équin, endocrine system, DIMETHYLFORMAMINE, Cryoprotectant, 040301 veterinary sciences, Semen, glycerol, Biology, Insemination, DMF, Andrology, Species Specificity, ETALON, Glycerol, conservation du sperme, Animals, Horses, CRYOPRESERVATION, urogenital system, 0402 animal and dairy science, fertilité animale, Dimethylformamide, Equidae, 040201 dairy & animal science, Sperm, anesse, Fertility, chemistry, Fertilization, Animal Science and Zoology, cheval, Semen Preservation
Abstract

International audience; A suitable method for the cryopreservation of donkey semen would be very valuable for the ex situ management of genetic diversity in this species. This report uses a variety of observation and trials to evaluate the effect of cryoprotectants in per-cycle pregnancy rates (PC) in equids females (jennies (donkey) and mares (horse)). This was explored by (1) comparing the results of insemination of jennies and mares with cooled or frozen donkey semen, (2) examining the possible toxic effect of the cryoprotectant (CPA) glycerol in these two species and (3) studying alternative solutions. Donkey and horse semen was either used immediately, or cooled according to some steps of the pre-freezing procedure or frozen and thawed. The pre-freezing procedure included semen dilution, centrifugation, resuspension in milk or in INRA82 + 2% egg yolk + various % CPA (expressed as final concentrations in extended semen (v/v)) and then cooling to 4 degrees C. PC was similar in mares and jennies inseminated with donkey semen cooled to 4 degrees C in milk. However, the PC was significantly higher in mares than in jennies when donkey semen was frozen with 2.2% glycerol (36%, n = 50 cycles vs. 11%, n = 38 cycles; P < 0.01). Increasing the concentrations of glycerol (0, 2.2, 3.5, 4.8%) before cooling stallion semen resulted in a progressive decrease in mare PC (87, 53, 53, 13% (n = 15 cycles for each concentration); P < 0.0001). The addition of 2.2% glycerol before cooling donkey semen decreased the PC measured in jennies to 0. The replacement of glycerol by 2% dimethylformamide increased the fertility obtained in jennies with cooled donkey semen (PC: 67%, n= 12 cycles) but did not increase the fertility obtained with frozen-thawed donkey semen (PC: 11%, n = 28 cycles with dimethylformamide vs. 0%, n = 16 cycles with glycerol). In conclusion, this study clearly shows that the ability of jennies to conceive after AI with donkey frozen semen is lower than that of mares. Glycerol affects the fertility of donkey and stallion spermatozoa as early as during the pre-freezing procedure. In consequence, the glycerol level must be low in frozen equine semen to provide good fertility. The toxic dose of glycerol for donkey spermatozoa seems to be almost half that for stallion spermatozoa. Whether this greater sensitivity of donkey spermatozoa to glycerol is responsible for the low success of semen cryopreservation in jennies is not so obvious because replacement of glycerol by dimethylformamide was not much more effective in terms of fertility. (C) 2008 Elsevier B.V. All rights reserved.

Published
2009
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4. La congélation de semence d'étalon dans un milieu à base d'INRA96® améliore le taux de fertilité en comparaison avec le milieu INRA82

Author

V. Furstoss, Florence Batellier, Elodie Pillet, Yves Le Vern, Dominique Kerboeuf, Guy Duchamp, Michèle Magistrini, Marianne Vidament, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), IMV Technologies, Unité Pluri-espèces d'Expérimentation Animale en Physiologie de la Reproduction et des Comportements (TOURS UPEA PRC), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), and Infectiologie Animale et Santé Publique (UR IASP)

Subjects
Cryoconservation, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, food.ingredient, Etalon, Total fertility rate, media_common.quotation_subject, reproduction animale, Semen, Fertility, Milieu INRA96, Biochemistry, Cryopreservation, law.invention, Andrology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, food, milieu de conservation, law, Yolk, Glycerol, conservation du sperme, media_common, Stallion, 030219 obstetrics & reproductive medicine, Chemistry, Extender, 0402 animal and dairy science, fertilité animale, 04 agricultural and veterinary sciences, 040201 dairy & animal science, Sperm, Agricultural sciences, cheval, semence, Sciences agricoles, Food Science, équin
Abstract

International audience; The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96 (R) extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96 (R) is a chemically- defined extender developed for fresh semen storage at 4 degrees C or 15 degrees C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96 (R) extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with INRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96 (R) than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96 (R). Further research is needed to understand how INRA96 (R) components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.

Published
2008
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5. [Effect of donor graft characteristics on clinical outcomes in Descemet stripping automated endothelial keratoplasty (DSAEK)].

Author

Ho Wang Yin G, Sampo M, Soare S, and Hoffart L

Subjects
Adult, Aged, Aged, 80 and over, Corneal Pachymetry, Corneal Transplantation methods, Female, Follow-Up Studies, France, Graft Survival, Humans, Male, Middle Aged, Prognosis, Treatment Outcome, Young Adult, Corneal Diseases diagnosis, Corneal Diseases surgery, Descemet Stripping Endothelial Keratoplasty methods, Endothelium, Corneal transplantation, Tissue Donors
Abstract

Introduction: The purpose of our study was to evaluate the progression of lamellar corneal grafts after endothelial keratoplasty by Descemet stripping automated endothelial keratoplasty (DSAEK) and the effect of their characteristics on the clinical and functional results., Materials and Methods: This was a prospective study in which 74 patients who had undergone endothelial keratoplasty by DSAEK were included. The corneal grafts were obtained from a French tissue bank and were delivered in a preservation medium containing a deturgescent agent (Corneajet ® or Stemalpha 3 ® ). Pachymetry of the corneal graft was measured ex vivo by ultrasonic pachymetry prior to dissection, then after the 1st or 2 nd cut as necessary. Corrected visual acuity in logMAR, total graft pachymetry, measured by anterior segment OCT (Spectralis ® HRA+SDOCT, Heidelberg engineering, Inc., Heidelberg, Germany) and corneal density (Pentacam ® , Oculus, Inc., Wetzlar, Germany) were obtained for each patient preoperatively, at D5, M1, M3, M6, M9 and M12., Results: Overall, 74 corneal grafts, from donors of mean age 69.7±13.3 years (37 to 92 years) were transplanted into 67 patients aged 70.3±12.1 years. Predissection pachymetry was statistically significantly thicker in the case of short deturgescence time (r=-0.383, P=0.001). For the same deturgescence time, predissection pachymetry was statistically significantly thicker in the Stemalpha 3 ® medium (691±89μm for Corneajet ® vs. 760±100μm for Stemalpha 3 ® , P=0.01), with the same final pachymetry (168±31μm vs. 166±25μm, P=0.833). Graft pachymetry and total corneal pachymetry decreased significantly between the preoperative measurement and M12 (168±29μm vs. 92±57μm, P=0.0008 et 665±124μm vs. 566±73μm, P=0.027 respectively). Preoperative endothelial cell density (2938±418 cells/mm 2 ) did not correlate with postoperative visual acuity. There was a positive correlation between visual acuity at D5 and donor age (r=0.3, P=0.04). Postoperative visual acuity correlated positively with preoperative visual acuity (P<0.05). Corneal densitometry was statically higher than normal postoperative densitometry. Corneal density at M1 correlated positively with time since obtaining the donor tissue (r=0.373, P=0.043). There was no significant difference between the sub-groups of over or under 130μm. Seven patients experienced graft failure with no statistically significant graft risk factor., Discussion: The preservation medium affects predissection pachymetry with no consequence on clinical results. In our study, preoperative visual acuity and donor age were correlated with visual acuity after DSAEK., Conclusion: Although donor age was associated with better visual acuity at D5, the other donor characteristics had no effect on clinical results., (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)

Published
2017
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