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We conducted the first nutritional analysis of dairy products from the traditional Roja Mallorquina sheep breed. Samples of bulk raw milk were taken twice a month from December 2015 to March 2016 from sheep fed using a part-time grazing system, and fresh soft (FC, n = 8) and ripened (RC, n = 8) cheeses were made. The variability in vitamins, total phenolic compounds (TPC), total antioxidant capacity (TAC), and fatty acid (FA) content was influenced by the cheese-making process (differences between the cheese and the original milk) and by the type of cheese-making technology (mainly related to heating, the use of starter culture, and ripening). The most notable physicochemical characteristic of the cheeses was low fat content (24.1 and 29.6 g/100 g for FC and RC). Milk and RC were characterised by major concentrations of retinol (211.4 and 233.6 μg/100 g dry matter (DM), respectively) and TPC (18.7 and 54.6 μg/100 g DM, respectively), while FC was characterised by major concentrations of retinol (376.4 μg) and α-tocopherol (361.7 μg). The fat-soluble components of the FC generally exhibited better nutritional value for human health than those of the milk and RC, with a higher level of retinol and α-tocopherol; lower values for saturated FA, atherogenic, and thrombogenic indices; and higher levels of monounsaturated FA, polyunsaturated FA, n-3, and n-6. Acids, alcohols, and ketones comprised almost 95% of the volatile compounds detected. Acetoin and products of lactose and citrate metabolism played an important role in the development of the aromatic attributes of both kinds of cheese. This preliminary study can contribute to add value to these traditional products according to healthy nutritional criteria and supports the implementation of strategies to promote their commercialisation and obtain product labelling as “pasture-fed” or specific marks.

Objectives. (1) To determine whether plasma dioxin-like (DL) bioactivity differs between Inuit living in different Greenlandic districts, (2) to compare the DL activity of the Inuit having high burdens of POPs with a low-burden study group (Danish volunteers), and (3) to evaluate DL activity associations to POP exposure biomarkers and/or lifestyle factors. Study design. The study was a cross-sectional survey, including randomized inhabitants (70) from 6 different Greenlandic districts and young Danish volunteers (22). Methods. POPs and fatty acids profiles were analysed. Aryl hydrocarbon receptor (AhR) mediated DL-activity was determined by exposure of Hepa1.12cR AhR-CALUX reporter cell line to hexane: ethanol plasma extracts. Results. The sum PCBs/POPs level of Inuit was more than10 times higher than the levels found in Danish volunteers, and for both study groups the level was positively associated with age. The TCDD equivalent of the determined DL-activity, AhR-TEQ, differed between the Greenlandic districts. The AhR-TEQ data of the combined Inuit was significantly higher compared with the Danish women. AhR-TEQ of Inuit were positively associated with plasma POPs after adjustment for age and/or the ratio of n-3 to n-6 fatty acids, whereas no correlations were found for the Danish samples. Conclusions. AhR-TEQ differs between Inuit and Danish plasma samples. Plasma POP levels alone cannot be used as a biomarker for DL-activity. We suggest that the profile and level of plasma POPs, geographical location and diet have the greatest impact on plasma dioxin activity. Further studies are needed to elucidate the differences in geographical determinants of blood DL-activity. [ABSTRACT FROM AUTHOR]

Objectives. (1) To determine whether plasma dioxin-like (DL) bioactivity differs between Inuit living in different Greenlandic districts, (2) to compare the DL activity of the Inuit having high burdens of POPs with a low-burden study group (Danish volunteers), and (3) to evaluate DL activity associations to POP exposure biomarkers and/or lifestyle factors. Study design. The study was a cross-sectional survey, including randomized inhabitants (70) from 6 different Greenlandic districts and young Danish volunteers (22). Methods. POPs and fatty acids profiles were analysed. Aryl hydrocarbon receptor (AhR) mediated DL-activity was determined by exposure of Hepa1.12cR AhR-CALUX reporter cell line to hexane: ethanol plasma extracts. Results. The sum PCBs/POPs level of Inuit was more than10 times higher than the levels found in Danish volunteers, and for both study groups the level was positively associated with age. The TCDD equivalent of the determined DL-activity, AhR-TEQ, differed between the Greenlandic districts. The AhR-TEQ data of the combined Inuit was significantly higher compared with the Danish women. AhR-TEQ of Inuit were positively associated with plasma POPs after adjustment for age and/or the ratio of n-3 to n-6 fatty acids, whereas no correlations were found for the Danish samples. Conclusions. AhR-TEQ differs between Inuit and Danish plasma samples. Plasma POP levels alone cannot be used as a biomarker for DL-activity. We suggest that the profile and level of plasma POPs, geographical location and diet have the greatest impact on plasma dioxin activity. Further studies are needed to elucidate the differences in geographical determinants of blood DL-activity. [ABSTRACT FROM AUTHOR]

Abstract: The aim of the present study was to evaluate the impact of dietary fatty acids (FA) during the weaning period on expression of genes involved in the oxidation and metabolism of FA (peroxisome proliferator-activated receptor (PPAR-α), stearoyl-CoA-desaturase (SCD), Δ6-desaturase (D6D), acetyl CoA carboxylase (ACC) and fatty acid synthase (FAS)). Liver samples were obtained from littermates, either on day 28 of age just before weaning, or at day 56 after the 4 weeks ad lib. provision of 5% of either animal fat, fish oil or sunflower oil. In conclusion, genes involved in the regulation of FA conversion (SCD, D6D) were influenced by the n-6 to n-3 ratio, whereas the FA oxidation, as indicated by the expression of PPAR-α, was highly likely regulated by the hepatic ratio between mono- and poly-unsaturated FA. Furthermore, weaning and/or age affected the hepatic expression of genes involved in FA synthesis and conversion, but not the expression of PPAR-α. [Copyright &y& Elsevier]

Red crab (Pleuroncodes planipes) is a marine crustacean that represents an abundant resource that has not been properly used. The aim of this study was to evaluate the effects on cholesterol and n-3 and n-6 FA content in eggs when red crab meal (RCM) was included in laying hen rations. Ninety White Leghorn laying hens were randomly distributed into 3 treatments: control (0%RCM), 3% red crab meal (3%RCM), and 6% red crab meal (6%RCM) for 3 wk. Cholesterol and linoleic (LA), α-linolenic (ALA), arachidonic (AA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids were analyzed by gas chromatography. Performance parameters and cholesterol content were not different among treatments (P > 0.05). Total lipids were different in the control group and 6%RCM (9.0 and 9.4% respectively) compared with 3%RCM (10.9%). With 3%RCM and 6%RCM, the levels of all fatty acids analyzed were higher than in the control. Diets with 3 and 6% RCM doubled the content of ALA (16.4 and 15.4 mg/100 g of egg, respectively). Eicosapentaenoic acid was lowest in 0%RCM (6 mg/100 g of egg) compared with 3%RCM (13 mg/100g) and 6%RCM (17 mg/100 g). Similar results were obtained for DHA (56, 133, and 246 mg/100 g of egg). Linoleic acid and AA were highest (1,211 and 228 mg/100 g, respectively) with 3%RCM and with 6%RCM (1,151 and 200 mg/100 g, respectively) vs. control (890 and 163 mg/100 g). The ratio of n-6 to n-3 FA was 3 times lower with 6%RCM than in the control. It was concluded that inclusion of red crab meal in laying hen rations at levels of 3 and 6% (P < 0.05) increased the n-3 and n-6 fatty acids content in eggs. [ABSTRACT FROM AUTHOR]

This study examined the pressurized solvent extraction of wheat germ oil. The effects of temperature (45–135 °C at 1500 psi), extraction time, sample size and solvent type on the extraction efficiency and oil quality were studied. Extraction efficiency of the normal-hexane was compared to that of the iso- and high purity-hexane, iso-propanol, ethanol and acetone. The extracts were analyzed for n-3 and n-6 polyunsaturated fatty acid content. Pressurized solvent extraction reduced extraction time significantly as compared to Soxhlet extraction. The time required for complete oil recovery was dependent on the amount of wheat germ used for extraction. The amount of extract collected was highest when ethanol was used as a solvent for pressurized solvent extraction. Soxhlet and pressurized solvent extractions resulted in similar oil yields when hexane was used as a solvent. Fatty acid composition of the extracts was not affected by either temperature or extraction method. The experimental results indicate that a pressurized solvent extraction technique reduces solvent consumption and extraction time with no adverse effect on the extraction yield and fatty acid composition of the oil. [Copyright &y& Elsevier]

There is considerable interest in the potentially protective effects of high fish consumption on many chronic diseases. Many epidemiological studies use food frequency questionnaires (FFQ) to quantify usual dietary fish intake, so it is important to validate this assessment against objective markers. The objective of this study was to determine the relationship between plasma percentage fatty acids and dietary fish intake as assessed by a FFQ. A semiquantitative FFQ was completed by 174 adults from the community (aged 26-49 years) who also had venous blood analysed for plasma percentage fatty acids. Following linear regression modelling, total non-fried fish intake was a significant predictor of n-3 (regression coefficient, B = 0.94; 95% CI = 0.60-1.28), docosahexaenoic acid (DHA; B = 0.73; 95% CI = 0.47-0.99) and the ratio of n-6: n-3 fatty acids (B = -1.0; 95% CI = - 1.35- -0.65). Steamed, grilled or baked fish was a small but significant predictor of eicosapentaenoic acid (EPA) levels (B = 0.13; 95% CI = 0.05-0.21) while total fish intake was a predictor of n-6 fatty acids (B = -0.88; 95% CI = -1.41- -0.36). This semiquantitative FFQ could be useful for ranking subjects according to their likely plasma n-3, DHA, and n-6 fatty acid intake and the ratio of n-6: n-3 fatty acids, when the available resources may simply not permit biological markers to be used. [ABSTRACT FROM AUTHOR]

We conducted the first nutritional analysis of dairy products from the traditional Roja Mallorquina sheep breed. Samples of bulk raw milk were taken twice a month from December 2015 to March 2016 from sheep fed using a part-time grazing system, and fresh soft (FC, n = 8) and ripened (RC, n = 8) cheeses were made. The variability in vitamins, total phenolic compounds (TPC), total antioxidant capacity (TAC), and fatty acid (FA) content was influenced by the cheese-making process (differences between the cheese and the original milk) and by the type of cheese-making technology (mainly related to heating, the use of starter culture, and ripening). The most notable physicochemical characteristic of the cheeses was low fat content (24.1 and 29.6 g/100 g for FC and RC). Milk and RC were characterised by major concentrations of retinol (211.4 and 233.6 μg/100 g dry matter (DM), respectively) and TPC (18.7 and 54.6 μg/100 g DM, respectively), while FC was characterised by major concentrations of retinol (376.4 μg) and α-tocopherol (361.7 μg). The fat-soluble components of the FC generally exhibited better nutritional value for human health than those of the milk and RC, with a higher level of retinol and α-tocopherol; lower values for saturated FA, atherogenic, and thrombogenic indices; and higher levels of monounsaturated FA, polyunsaturated FA, n-3, and n-6. Acids, alcohols, and ketones comprised almost 95% of the volatile compounds detected. Acetoin and products of lactose and citrate metabolism played an important role in the development of the aromatic attributes of both kinds of cheese. This preliminary study can contribute to add value to these traditional products according to healthy nutritional criteria and supports the implementation of strategies to promote their commercialisation and obtain product labelling as "pasture-fed" or specific marks. [ABSTRACT FROM AUTHOR]