Tree mortality is a key factor for understanding forest dynamics. So far, however, only few studies have focused on the mortality of tree regeneration. Thus, more research is needed in this context to better understand the underlying ecological processes and predict more reliably how forest ecosystems will respond to ongoing climate change. As the current and future changes are expected to either favor or impair the growth and survival of large trees, it is pivotal to investigate the effects on the next generation, i.e. young and small trees. A better understanding of tree regeneration allows to extend or adapt the findings from the already well-studied adult, large trees to small-sized trees, for which sparse empirical data are available. Particularly relevant questions relate to the drivers of survival probabilities and of horizontal spatial patterns (i.e. the distribution in space) of tree regeneration. The aims of this PhD thesis therefore were (i) to improve the understanding of natural mortality processes of tree regeneration at different developmental stages and spatio-temporal scales, (ii) to investigate the effect of various abiotic and biotic factors on the mortality of tree regeneration, and (iii) to study the relationship between mortality, growth and site conditions. For this purpose, I assessed 1) the effect of emergence time, height and number of leaves of seedlings, light availability, temperature, precipitation, seedbed and microsite conditions on the survival time of seedlings; 2) the effect of light availability on the growth of saplings and its relationship with mortality; and 3) the effect of topography and of large neighboring trees on the spatial pattern of living and dead small trees. These effects were investigated for small-sized trees (diameter at breast height < 10 cm) of both conifer and deciduous species at several study sites in Switzerland across elevational gradients that represent distinct climate regimes and growth conditions. In Chapter 1, I studied the effects of emergence time on the mortality of seedlings. The underlying rationale was that global warming is expected to advance the timing of germination, leading the seedlings to potentially experience more severe damage and mortality due to late frost events in spring. Thus, I monitored the emergence, characteristics, and survival of seedlings across ten tree species in temperate mixed deciduous forests around Zurich (Switzerland) over one and a half years. For each seedling, I recorded characteristics such as height, number of cotyledons and euphylls, cause and severity of possible damages, and extent of missing foliar tissue due to herbivory. Moreover, I documented the seedbed type of each seedling and that of the microsite at the plot level. For each plot, I also determined light availability using hemispherical canopy photographs, logged the temperature curve, and measured soil moisture. Based on the empirical data, I conducted a survival analysis using the Kaplan-Meier method to estimate survival curves and Cox’s proportional hazards model to assess the effects of the explanatory variables on survival time. I tested whether the timing of emergence represents a trade‐off for seedling survival between minimizing frost risk and maximizing the length of the growing period. Seedlings that emerged early faced a severe late frost event. Nevertheless, they benefited from the overall longer growing period, resulting in increased overall survival. Larger seedling height and higher number of leaves positively influenced survival. Seedlings growing on moss had higher survival compared to those growing on mineral soil, litter, or in herbaceous vegetation. Since almost two‐thirds of the monitored seedlings died during the first growing season and early-emerging seedlings were more likely to survive, this chapter highlights how the first months of life together with an early emergence time of seedlings are decisive for successful tree regeneration, which will ultimately have an impact on the future development of forest stands. In Chapter 2, I investigated whether radial and vertical growth rates are suitable indicators of impending mortality in young trees, as previous research on adult, large trees had suggested, and whether light availability and tree size have an influence on mortality probability. Thus, I sampled an equal number of living and dead saplings of four conifer species (Swiss stone pine, European larch, Norway spruce and silver fir) in nine mountain forests along an elevational gradient of the Swiss Alps. I performed a tree-ring analysis, calculated both radial and vertical growth rates and compared them between living and dead saplings based on tree-ring widths reconstructed from stem disks at multiple tree heights. I observed a divergent pattern in radial growth of living and dead saplings, with reduced growth of dead saplings starting several years prior to death, which emphasizes the importance of long-term predisposing factors for tree mortality. Then, I quantified the combined effects of light availability, growth and tree size on mortality, using species- and site-specific conditional logistic regression models, by previously matching living and dead saplings of similar ages. Light availability influenced positively the survival probabilities of conifer saplings in mountain forests, although the positive effect decreased with increasing elevation. Recent radial growth rate and diameter had only minor effects on sapling mortality. By highlighting the importance of long-term predisposing factors for the mortality of conifer saplings in mountain forests, this chapter extends well-established findings of the adult stage to the so far little investigated sapling stage. In Chapter 3, I analyzed the horizontal spatial patterns of small living and dead Norway spruce trees in two subalpine forest reserves of Switzerland, Scatlè and Bödmerenwald, by nearest neighbor-based and distance-based analyses. I accounted for spatial inhomogeneity by investigating how the local densities of living and dead small trees depend on environmental covariates. I found that the local density of living and dead small trees is influenced by latitude, elevation and aspect. Yet, the influence of these covariates varied between the two forest reserves due to their different topography and peculiar site conditions. Then, I considered neighborhood interactions between trees based on the vicinity and size of trees, by analyzing how small trees are influenced by large neighboring trees over a range of spatial scales. Both tree vicinity and size were important for the spatial patterns of small trees in both reserves. Small living trees showed a random pattern around large dead trees over a range of distances and, at certain distances in one reserve, even dispersion. Small living trees further showed clustering around large living trees at short distances and dispersion at large distances. Small dead trees featured mainly a random pattern, even though with a tendency to cluster at short distances around large neighbors, irrespective of whether these were living or dead. Yet, the fading of clustering with increasing distance indicates that the influence of large trees on small trees varies with the distance and thus that the neighborhood interactions between trees are scale-dependent. I further found that the influence of large neighboring trees on small trees varied with topography, revealing a relationship between spatial inhomogeneity and neighborhood interactions, as I expected due to the strongly different tree sizes and environmental gradients in mountain forests. Overall, this chapter emphasizes the importance of considering both spatial inhomogeneity and neighborhood interactions when investigating the spatial ecology of mortality of small-sized trees in uneven-aged and unmanaged mountain forests. Throughout this PhD thesis, I extended well-established ecological findings from the adult, large trees to the regeneration stage of trees, which is an important bottleneck of forest dynamics. The empirical findings of my PhD thesis represent a considerable contribution towards a better understanding of the temporal and spatial patterns of mortality in tree regeneration as well as of the relationship between mortality, growth and site conditions.