1. Development of the screening conditions in vitro effective drugs against the causative agent of Сhikungunya fever
- Author
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S. Ya. Loginova, V. N. Schukina, S. V. Savenko, R. V. Sakharov, I. V. Surovyatkina, and S. V. Borisevich
- Subjects
сhikungunya virus ,cell culture ,multiple infections ,negative colonies ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The Сhikungunya virus (CHIKV) is a member of the Flavivirus genus, the Flaviviridae family, and is a zoonotic arbovirus infection transmitted by Aedes mosquitoes. Chikungunya fever causes explosive epidemics involving millions of cases. There is an urgent need to develop broad-spectrum antivirals. Actual is the development of a model of screening in vitro effective drugs. To select the optimal screening conditions, a comparative analysis of cell cultures sensitivity to Сhikungunya virus, strain FN198/66 was carried out. The dynamics and level of virus accumulation at different infectious doses were estimated. It is shown that the Сhikungunya virus, strain FN198/66, well propagated in transplantable cell cultures Vero V, Сl008 Vero, BHK-21/13 and TC-17. All studied cell lines can be used to screen for effective non-specific medical defenses against Сhikungunya virus.
- Published
- 2023
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