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2 results on '"noninvasive DNA isolation"'

1. Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment

Author

Min Zhang, Min Wei, Zhiguo Dong, Haibao Duan, Shuang Mao, Senlei Feng, Wenqian Li, Zepeng Sun, Jiawei Li, Kanglu Yan, Hao Liu, Xueping Meng, and Hongxing Ge

Subjects
Cyclina sinensis, Feces, noninvasive DNA isolation, DNA degradation, Biotechnology, TP248.13-248.65
Abstract

Abstract Background To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality of fecal DNA. And we analyzed the effects of different conditions on the degradation of feces and fecal DNA. Results The clear fecal DNA bands were detected by electrophoresis, and PCR amplification using clam fecal DNA as template was effective and reliable, suggesting that clam feces can be used as an ideal material for noninvasive DNA isolation. In addition, by analyzing the effects of different environmental temperatures and soaking times on the degradation of feces and fecal DNA, we found that the optimum temperature was 4 °C. In 15 days, the feces maintained good texture, and the quality of fecal DNA was good. At 28 °C, the feces degraded in 5 days, and the quality of fecal DNA was poor. Conclusions The clam feces can be used as an ideal material for noninvasive DNA isolation. Moreover, the quality of fecal DNA is negatively correlated with environmental temperature and soaking time.

Published
2019
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2. Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment

Author

Hongxing Ge, Hao Liu, Jiawei Li, Dong Zhiguo, Shuang Mao, Xueping Meng, Feng Senlei, Min Zhang, Duan Haibao, Sun Zepeng, Kanglu Yan, Li Wenqian, and Min Wei

Subjects
0106 biological sciences, 0301 basic medicine, Gel electrophoresis of nucleic acids, lcsh:Biotechnology, Biology, 010603 evolutionary biology, 01 natural sciences, Polymerase Chain Reaction, law.invention, 03 medical and health sciences, chemistry.chemical_compound, Feces, Environmental temperature, fluids and secretions, law, lcsh:TP248.13-248.65, Cyclina sinensis, Animals, Food science, Polymerase chain reaction, DNA Primers, DNA degradation, DNA, noninvasive DNA isolation, DNA extraction, Bivalvia, 030104 developmental biology, chemistry, Degradation (geology), Biotechnology, Research Article
Abstract

Background To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality of fecal DNA. And we analyzed the effects of different conditions on the degradation of feces and fecal DNA. Results The clear fecal DNA bands were detected by electrophoresis, and PCR amplification using clam fecal DNA as template was effective and reliable, suggesting that clam feces can be used as an ideal material for noninvasive DNA isolation. In addition, by analyzing the effects of different environmental temperatures and soaking times on the degradation of feces and fecal DNA, we found that the optimum temperature was 4 °C. In 15 days, the feces maintained good texture, and the quality of fecal DNA was good. At 28 °C, the feces degraded in 5 days, and the quality of fecal DNA was poor. Conclusions The clam feces can be used as an ideal material for noninvasive DNA isolation. Moreover, the quality of fecal DNA is negatively correlated with environmental temperature and soaking time.

Published
2019

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