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Background. The most effective and environmentally safe way to combat wheat diseases is to produce cultivars resistant to their pathogens. For this purpose, old landraces are often used as genetically diverse sources of traits important for breeding. In the process of wheat breeding for resistance to tan spot caused by the fungus Pyrenophora tritici-repentis (Died.) Drechs. (abbr. Ptr), selection is carried out against the dominant allele of Tsn1, the gene of sensitivity to the toxin Ptr ToxA, which induces necrosis and represents the main pathogenicity factor of Ptr controlled by the ToxA gene. The aim of the study was to characterize a set of bread wheat (Triticum aestivum L.) accessions from the VIR collection for resistance to various Ptr populations, genotype these accessions using Xfcp623 – a DNA marker of the Tsn1 gene, and identify sources of tan spot resistance.Materials and methods. Sixty-seven accessions of winter bread wheat landraces were studied. Seedling resistance to two Ptr populations was assessed using a 5-point scale adopted at VIZR. The allelic state of Tsn1 was identified by PCR.Results. Dominant alleles of Tsn1 were found for 55% of the studied accessions. Seventeen accessions were resistant or moderately resistant to two Ptr populations and an isolate from Krasnodar Territory previously used for their characterization. Nine of them had the tsn1tsn1 genotype, and 8 had Tsn1Tsn1. The accessions mainly belonged to three agroecological groups proposed by N. I. Vavilov: “steppe winter bread wheat (Banatka wheats)”, “North European forest awnless bread wheats (Sandomirka wheats)”, and “Caucasian mountain winter bread wheat”.Conclusion. The identified 17 accessions resistant to Ptr are potential breeding sources of resistance. In the studied set of accessions, no significant relationship was found between the allelic state of the Tsn1 gene in the accession and its response to the infection with pathogen populations, including isolates with the ToxA gene.

Keywords: breeding for resistance; durability of resistance; pathogen populations; resistance location; integrated management; yield; quality; wheat EN breeding for resistance durability of resistance pathogen populations resistance location integrated management yield quality wheat N.PAG N.PAG 5 04/10/21 20210330 NES 210330 Introduction to Wheat Diseases Agriculture in 2050 will need to produce about 50% more food because of the increase in the world population and the change in diets (FAO, [5]). Furthermore, 79 genes and more than 200 QTL were found for leaf rust and 82 genes and 140 QTL for stripe rust in the seedling and adult stages, respectively. Changes of races and virulence genes in Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen, in the United States from 1968 to 2009. Fabre et al. reported that some crop mechanisms and the expression of specific genes are needed to stimulate this disease, reviewing recent findings on the existence of wheat susceptibility factors, the role of phytohormones on its development and the role of the effectors such as DON in the susceptibility. [Extracted from the article]

Washing soft fresh produce such as strawberries, baby leaves, and sliced onions with sanitizing agents is challenging due to their fragile texture. Thus, treatments like aerosolization using slightly acidic electrolyzed water (SAEW) and ultraviolet C light-emitting diode (UVC LED) irradiation may be good alternatives. In the present study, the reduction effects of a combined treatment of aerosolization using SAEW and UVC LED irradiation on enterohemorrhagic Escherichia coli (EHEC) and Staphylococcus aureus attached to strawberries, baby leaves, and sliced onions were investigated. The behaviours of EHEC and S. aureus, moisture loss, colour measurement, and visual appearance were also analyzed at 10 and 15 °C for 7 days. The reduction effect of the combined treatment with 100 SAEW and UVC LED was higher (0.53–0.92 log CFU g−1) than a single aerosolization treatment (0.11–0.41 log CFU g−1), regardless of samples or pathogens. A greater effect on EHEC and S. aureus reduction was observed in strawberries (0.74 and 0.92 log CFU g−1) than in baby leaves (0.62 and 0.53 log CFU g−1) and sliced onions (0.55 and 0.62 log CFU g−1). The combined treatment further reduced the EHEC and S. aureus populations in strawberries during 7 days of storage at 10 and 15 °C. However, the EHEC and S. aureus populations were maintained in baby leaves and sliced onions at 10 °C for 7 days. Additionally, the greatest effect on the maintenance of colour and appearance was obtained in the combined treatment. Since the combined treatment reduces EHEC and S. aureus populations and preserves visual quality, it could be expected to extend the shelf life of soft fresh produce at the retailer stage of the supply chain.

Virulence frequencies to powdery mildew resistances in winter barley cultivars mostly registered in the Czech Republic were studied in 2007 and 2008. Random samples of the air populations originating from winter and spring barley fields were obtained by means of a mobile version of a jet spore sampler. Conidia were sampled by driving across the Czech Republic. In total 349 isolates were studied and 17 differentials were used. The virulence frequencies to specific resistances of given cultivars showed wide range from 0% to 100%. Nine differentials were used to distinguish 134 pathotypes, of which 32 representing 63.9% of isolates were detected in both years. Pathotype 773, which broke down the resistance of eight differentials, was the most abundant. In 2008, lower virulence frequencies to all differentials, and thus lower population complexity, were determined, which may be caused by different regional origins of the isolates examined. Importance of the study of the given pathogen population is discussed in terms of successful breeding of resistant barley cultivars.

The virulence frequencies to powdery mildew resistance genes possessed by winter barley cultivars registered and newly tested in the Czech Republic were studied in 2000, 2002 and 2004. Random samples of the populations originating from winter and spring barley fields were obtained from the air by a mobile version of a jet spore sampler mounted on a car roof. Conidia were sampled by driving across the Czech Republic. Fourteen differentials, carrying 18 out of 20 currently identified resistance genes present in winter barley cultivars, were used. High virulence frequencies (85-100%) to most resistance genes were found. Lower virulence frequencies (14.1-40.1%) were found to only three resistance genes that have not been described yet; their preliminary designations are Ml(Va), Ml(Dt) and Ml(Ca). The importance of resistance of winter barley cultivars is discussed with respect to limiting the speed with which the pathogen adapts to genetic resistances possessed by commercial cultivars of both winter and spring barley, and to the necessity of lowering the costs for powdery mildew control in barley.

Virulence analysis and two polymerase chain reaction-based assays were used to evaluate the population structure of Xanthomonas oryzae pv . oryzae (Xoo) from different elevations ranging from 150 to 2600 m in south-west China. Among the 218 isolates of Xoo, 18 pathotypes were identified using six near-isogenic rice lines, each containing a single resistance gene. Among them, pathotype 9 predominated in low and mid-elevations was virulent to all resistance genes, including Xa2, Xa3, xa5, xa13, Xa14 and Xa18. However, pathotype 2 was predominant at high elevation and was virulent to Xa18 only. The 18 pathotypes were grouped into four clusters. Isolates belonging to cluster 1 were mainly found at high and mid-elevations, while those of cluster 4 were mainly found at low elevations. There were significant trends of virulence of isolates from low to high with the elevation from high to low. The ERIC and J3 primers were used to screen the genomes of 218 isolates, and 56 molecular haplotypes were found. Multiple correspondence analyses revealed that 56 haplotypes were divided into four putative genetic lineages. Lineage 2 was the most frequently detected from 150 to 2600 m; it was clearly shown that isolates from high elevation with 80% is much more than from low and mid-elevation in the lineage. It is intriguing that genetic variation of Xoo is restricted by physical geographical barriers of elevations. This is the first report on the relationship of pathotypic and genotypic diversity of Xoo at different elevations. [ABSTRACT FROM AUTHOR]

In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of clonal lineages, with a wide range of haplotypes. M. oryzae populations lacked the grasshopper ( grh) element (96%) and appeared distinct to those in Asia. An overall near equal distribution (47-53%) of the mating types MAT1- 1 and MAT1- 2, high fertility status (84-89%) and the dominance of hermaphrodites (64%) suggest a strong sexual reproductive potential. Differences in pathogen aggressiveness and lack of cultivar incompatibility suggest the importance of quantitative resistance. Rice blast populations in West Africa showed a typical lineage-based structure. Among the nine lineages identified, three comprised ~90% of the isolates. Skewed distribution of the mating types MAT1- 1 (29%) and MAT1- 2 (71%) was accompanied by low fertility. Clear differences in cultivar compatibility within and between lineages suggest R gene-mediated interactions. Distinctive patterns of genetic diversity, sexual reproductive potential and pathogenicity suggest adaptive divergence of host-specific forms of M. oryzae populations linked to crop domestication and agricultural intensification. [ABSTRACT FROM AUTHOR]

Copyright of American Journal of Potato Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of American Journal of Potato Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Washing soft fresh produce such as strawberries, baby leaves, and sliced onions with sanitizing agents is challenging due to their fragile texture. Thus, treatments like aerosolization using slightly acidic electrolyzed water (SAEW) and ultraviolet C light-emitting diode (UVC LED) irradiation may be good alternatives. In the present study, the reduction effects of a combined treatment of aerosolization using SAEW and UVC LED irradiation on enterohemorrhagic Escherichia coli (EHEC) and Staphylococcus aureus attached to strawberries, baby leaves, and sliced onions were investigated. The behaviours of EHEC and S. aureus, moisture loss, colour measurement, and visual appearance were also analyzed at 10 and 15 °C for 7 days. The reduction effect of the combined treatment with 100 SAEW and UVC LED was higher (0.53–0.92 log CFU g−1) than a single aerosolization treatment (0.11–0.41 log CFU g−1), regardless of samples or pathogens. A greater effect on EHEC and S. aureus reduction was observed in strawberries (0.74 and 0.92 log CFU g−1) than in baby leaves (0.62 and 0.53 log CFU g−1) and sliced onions (0.55 and 0.62 log CFU g−1). The combined treatment further reduced the EHEC and S. aureus populations in strawberries during 7 days of storage at 10 and 15 °C. However, the EHEC and S. aureus populations were maintained in baby leaves and sliced onions at 10 °C for 7 days. Additionally, the greatest effect on the maintenance of colour and appearance was obtained in the combined treatment. Since the combined treatment reduces EHEC and S. aureus populations and preserves visual quality, it could be expected to extend the shelf life of soft fresh produce at the retailer stage of the supply chain. [ABSTRACT FROM AUTHOR]