1. Gene expression response of Litopenaeus vannamei crustins in two specialized immune-related tissues upon Vibrio immersion challenge.
- Author
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Argenta, Nicolas, Perazzolo, Luciane Maria, and Rosa, Rafael Diego
- Subjects
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WHITELEG shrimp , *GENE expression , *VIBRIO , *VIBRIO harveyi , *GRAM-negative bacteria - Abstract
The aim of this study was to characterize the transcriptional response of representative genes from the four crustin types (Type I, LvCrustin Ia-6 and LvCrustin Ia-8; Type II, Crustin Lv and Crustin-like Lv; Type III, LvSWD; and Type IV, LvSLPI) in circulating hemocytes and intestine of Litopenaeus vannamei shrimp using an immersion-based challenge with the Gram-negative pathogen Vibrio harveyi. Tissue distribution pattern analysis showed that transcripts of both Type I crustins and Crustin-like Lv were only quantifiable in the intestine and hemocytes, respectively. Comparatively, the basal mRNA levels of Crustin Lv, LvSWD, and LvSLPI were higher in circulating hemocytes than in the intestine. While the expression of Crustin Lv increased 2.4-fold change in the intestine at 12 h after the bacterial immersion challenge, the transcriptional levels of the other crustin genes remained unaltered in the analyzed immune-related tissues. In conclusion, crustins from L. vannamei exhibit a distinct pattern of basal gene expression in hemocytes and intestine and are differentially modulated in response to a Vibrio immersion challenge. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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