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The experiment was conducted from April to May 2024 at the Centre for Excellence in Biotechnology Research and Training (CEBRT) lab of Amicable Knowledge Solution University (AKS) University, Satna. In the present study, a simple, routine, and reproducible RP-HPLC method was employed for estimating the levels of Ferulic acid (FA) in different treatments of maize that is essential in the phytochemical analysis of this plant, has been assessed based on ferulic acid (FA). The method used in this study was based on HPLC on a C18 column using a gradient of water, methanol, and acetic acid. The calibration curve showed optimum linearity from 10 to 100 µg ml-1, with R²=0.981. This method is used to quantify the concentration of FA. This methodology was used to detect FA in maize plants with different treatments: T1: control plants, T2: drought-induced plants, T3: plants inoculated with PKR1, T4: plants inoculated with PKR5, T5: plants inoculated with PKR6. An increase in FA was observed in all three Plant Growth-Promoting Rhizobacteria (PGPR)-treated plants (T3, T4, and T5) compared to T1 (control plants) and T2 (drought-induced plants). Among these, maximum FA was found in T4 treatment, and the maximum peak height and area of T4 were 229259 and 10884, respectively. The validated RP-HPLC method provided a reliable approach for analyzing FA content in maize leaves, supporting phytochemical studies and quality assurance across various plant treatments and derived products. [ABSTRACT FROM AUTHOR]

BACKGROUND: This study investigated the effects of γ‐aminobutyric acid (GABA) combined with ultrasonic stress germination (AUG) treatment on the phenolic content and antioxidant activity of highland barley (HB). Key variables, including germination times (ranging from 0 to 96 h), ultrasonic power (200–500 W), and GABA concentration (5–20 mmol/L), were optimized using response surface methodology (RSM) to enhance the enrichment of phenolic compounds. Furthermore, the study assessed the content, composition, and antioxidant activities of phenolic compounds in HB under various treatment conditions such as germination alone (G), ultrasonic stress germination (UG), and AUG treatment. RESULTS: The study identified optimal conditions for the phenolic enrichment of HB, which included a germination time of 60 h, an ultrasound power of 300 W, and a GABA concentration of 15 mmol L−1. Under these conditions, the total phenolic content (TPC) in HB was measured at 7.73 milligrams of gallic acid equivalents per gram dry weight (mg GAE/g DW), representing a 34.96% enhancement compared to untreated HB. Notably, all treatment modalities – G, UG, and AUG – significantly increased the phenolic content and antioxidant activity in HB, with the AUG treatment proving to be the most effective. CONCLUSION: These obtained results suggest that AUG treatment is a promising processing method for enriching phenolic compounds and improving antioxidant activity in HB. Subsequently, the AUG‐treated HB can be used to develop phenolic‐rich germinated functional foods to further broaden the application of HB. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Polygonum multiflorum Thunb. is an important medicinal plant with various biologically active substances such as emodin, THSG (2,3,5,4’-tetrahydroxystilben-2-O-β-D-glucoside), phenolics, flavonoids, and others. This study evaluated the effectiveness of different sterilization methods and optimized culture conditions for the rapid in vitro propagation of P. multiflorum. Silver nanoparticles (AgNPs) combined with Medipag was the most effective sterilization method compared to individual treatments for the same duration of 30 min. Subsequently, Javel was applied for 27 min to achieve further sterilization. After two weeks, the highest regeneration rate observed was 58.0%. The maximum number of shoots were induced from bud explants on MS (Murashige and Skoog, 1962) medium containing 4.0 mg/L BAP + 0.1 mg/L NAA with an average of 5.40 shoots/explant and shoot height of 20.40 mm after four weeks of culture. The highest rooting was observed in ¾ MS medium supplemented with 0.5 mg/L NAA with an average of 8.60 roots/shoot and 7.06 cm root length after five weeks of culture. Moreover, we observed that the tuber of P. multiflorum cultivated ex vitro possessed a superior total phenolic content (58.27 mg/g DW) and total flavonoid content (37.26 mg/g DW) compared to other plant parts. Similarly, the tuber extract showed the highest antioxidant activity at 94.64%. Notably, the amounts of these compounds were higher in in vitro shoots than in ex vitro leaves and stems. There was also a strong correlation between the total phenolic and flavonoid contents (r² = 0.991) and antioxidant activity. This study successfully established a comprehensive micropropagation protocol from initial explant cultivation to plantlet acclimatization under natural conditions. It further assessed the advantages of tissue-cultured plants in terms of phenolic and flavonoid compound accumulation, and their antioxidant capacity.Key message: This study developed a complete micropropagation protocol through plantlet acclimatization in natural conditions, while also highlighting the advantages of tissue-cultured plants in secondary compound accumulation and antioxidant activity. [ABSTRACT FROM AUTHOR]

Bananas are one of the most extensively cultivated fruits globally, yielding substantial amounts of greenery, including their leaves. Banana leaves (BL) have notable protein content, bioactive compounds, and a significant fiber component. This study aimed to investigate the impact of incorporating dried and nutrient-determined BL along with a multienzyme complex into the diet of quails. The experiment lasted 35 days, a total of 280 one-day-old quail chicks (Coturnix coturnix japonica) were allocated into four groups with seven replicates. Experimental diets were formulated with the addition of dried BL at levels of 0% (C), 3% (BL3), 6% (BL6), and 9% (BL9), with the inclusion of 1,000 mg/kg multienzyme complex to the basal diet. The total fiber content of diets was determined at 2.77%, 4.28%, 5.77%, and 7.28%, respectively. The inclusion of BL in the diet did not significantly affect growth performance. However, the addition of 3% and 6% BL to the diet resulted in a significant reduction in meat oxidation. A 6% BL inclusion led to the lowest serum low-density lipoprotein and the highest high-density lipoprotein concentrations (p < 0.05). Meat yellowness (b*) increased with all three levels of BL in the diet compared to the basal diet (p < 0.05), while L* and a* values remained unaffected. A 6% BL addition to quail diets may lead to improved meat quality and higher serum HDL concentration without detrimental effects on growth performance. [ABSTRACT FROM AUTHOR]

The excessive use of chemical fertilizers in the Guanxi honey pomelo production area has led to severe soil acidification and magnesium (Mg) deficiency, adversely affecting pomelo fruit quality. To address this issue, an integrated nutrient optimization model crucial for ensuring the sustainable and environmentally friendly development of the Guanxi honey pomelo industry has been explored. In a three-year experiment, two fertilizer treatments were implemented: a farmer fertilizer practice (FP) and an NPK reduction plus foliar Mg fertilizer (OPT + fMg). We investigated the impact of this integrated optimized fertilization measure on pomelo fruit quality from three aspects: flavor (sugars and organic acids), nutrition (vitamin C and mineral elements), and antioxidant properties (phenolics, flavonoids, and phytic acid). The results revealed that the OPT + fMg treatment improved fruit flavor by reducing acidity (titratable acid, citric acid, and quinine), while having a minimal impact on sugar components (sucrose, fructose, and glucose). Additionally, the OPT + fMg treatment increased the total phenolics, total flavonoids, and phytic acid in the fruit peel, enhancing its potential antioxidant quality. However, the OPT + fMg treatment reduced the mineral nutrient quality (excluding calcium) in the fruit. As for the fruit developmental period, the OPT + fMg treatment significantly increased the total flavonoid concentration in the peel from the mid-expansion fruit stage, followed by notable increases in phytic acid in the peel during the mid-to-late expansion fruit stage. The total phenolic concentration in the peel significantly rose only during the late fruit development stage. The most pronounced effect was observed on phytic acid in both peel and pulp. The influence of the OPT + fMg treatment on the mineral nutrients (excluding calcium) primarily occurred during the mid-to-late expansion fruit stage. Overall, the OPT + fMg treatment significantly improved the comprehensive nutritional quality of pomelo fruit, providing valuable insights for scientifically reducing fertilizer application while enhancing fruit quality. [ABSTRACT FROM AUTHOR]

Food waste is a significant concern when it comes to food safety. It is a well-known fact that fruit and vegetable wastage is high worldwide; however, quantitative data, especially on such waste in the retail sector, are limited. Wasted vegetables are sources of essential dietary compounds, benefiting from their revalorization. Studies related to the evaluation of the quantity and quality of these vegetables discarded in the retail sector can allow for the proposal of relevant alternatives for their use and the guarantee of food safety. This study aimed to assess wasted vegetables (beetroot, carrot, chayote, and cucumber) in the city of Pirassununga (Brazil) and characterize the purees obtained from these vegetable wastes. The purees were characterized in terms of microbiological aspects, color, proximal and mineral composition, phenolic concentration, and antioxidant activity. It was observed that 90% of the discarded vegetables were free from microbiological contamination and could be considered suitable for consumption according to the adopted classifications. Additionally, the purees had high nutritional levels, such as phenolics and fiber. Thus, considering the high levels of vegetable waste generated in the retail sector, and high value nutritional, vegetable waste could be viable in the development of new products, making it an important retail strategy for the circular economy. [ABSTRACT FROM AUTHOR]

Considering the areas of use of phenolic compounds, it is important to determine the concentrations at which they show synergistic and antagonistic interactions for their integration into various systems and their correct use. In this study, the synergistic interaction concentration of rosmarinic acid, gallic acid, and caffeic acid was determined by Folin--Ciocalteu and FRAP methods. The central composite design--response surface methodology was used to determine the optimum concentration for synergistic interaction. As a result of the optimization, caffeic acid, rosmarinic acid, and gallic acid showed synergistic interaction at 7.87 μM, 6.75 μM and 9.42 μM concentrations for Folin--Ciocalteu method; 8.03 μM, 9.34 μM and 6.00 μM concentration for FRAP method respectively. The capacity of phenolic compounds to prevent the formation of DNA base damage products was evaluated by GC--MS/MS. As a result, the synergistic concentration of three phenolics reduces the DNA damage products at 37.17% (FOLIN) and 40.17% (FRAP). [ABSTRACT FROM AUTHOR]

The influence of selected yeast starters (Kluyveromyces lactis L39, Kazachstania humilis AG5, Nakazawaea molendinolei IG9, Candida diddensiae IG12, and Candida adriatica L30) on the fermentation and final characteristics of natural-style Gordal and Manzanilla green table olives was investigated. In both cultivars, the addition of yeast starters had no significant influence on the evolution of physicochemical parameters or the final main metabolites compared to noninoculated olives. In the Gordal cultivar, K. lactis L39 originated the greatest enrichment of volatile compounds, whereas K. lactis L39 and C. adriatica L30 gave the best volatile profiles in the Manzanilla cultivar. In both cultivars, the β-glucosidase-positive strains N. molendinolei IG9, C. diddensiae IG12, and C. adriatica L30 produced no significant decrease in the total phenolic content at the end of fermentation. Although the yeast starters had a significant effect on the volatile contents of the fermented products, they did not have a significant influence on the main sensory characteristics perceived by a sensory panel. A significant linear relationship (R2 = 0.815, p < 0.001) was found and validated between the perceived bitterness intensity and the content of total phenols in olive pulp, providing a simple and objective method for the evaluation of bitterness in table olives without the need for sensory analysis. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the effects of the solvent type, extraction time, extraction temperature and solvent-to-material ratio on the recovery of the total saponin content (TSC), the total phenolic content (TPC) and the antioxidant activity from the rhizomes of Curculigo orchioides Gaertn. The extraction efficiency of the bioactive compounds by ultrasound-assisted extraction (UAE) was also evaluated. Extraction with 80% ethanol achieved the highest extraction yields, and the optimal conditions for the extraction of TSC were 60 min, 57 °C and an 80 mL/g solvent-to-material ratio. The optimal conditions for the recovery of TPC were 178 min, 45 °C and a solvent-to-material ratio of 68 mL/g. The highest antioxidant activity of the extracts from Curculigo orchioides Gaertn rhizomes was obtained with the optimal conditions of 180 min, 40 °C and an 80 mL/g solvent-to-material ratio. The actual extraction yields obtained from the optimal conditions were 11.33 mg aescin equivalents (AE)/g dry weight (DW) for TSC, 23.58 mg gallic acid equivalents (GAE)/g DW for TPC and DPPH antioxidant activity of 133.45 µM Trolox equivalents (TE)/g DW. UAE using the same type and amount of solvent for only 10 min could result in comparable extraction yields of TSC, TPC and DPPH antioxidant activity to the 180 min conventional extraction process. These promising results suggest the potential for the development of effective extraction processes to recover bioactive compounds from Curculigo orchioides Gaertn rhizomes in practical production. [ABSTRACT FROM AUTHOR]

Spray-dried yellow cashew juice ingredients produced under different inlet temperatures (140 and 150 °C) and gum arabic (GA) addition ratios (15% and 25% w/v) were evaluated for their physicochemical and phytochemical attributes and storage stability for 56 days. All spray-dried cashew juice particles showed high solids recovery (>70%) and solubility (>90%), low water activity (<0.3), and low hygroscopicity (<10%). Spray-dried particles prepared with 15% w/v GA showed spherical shapes with a semi-crystalline structure and higher ascorbic acid concentration (>650 mg 100 g−1) and total phenolic content (>330 mg GAE 100 g−1). During storage, spray-dried cashew juice particles maintained their water activity levels within the microbiologically safe range and retained high solubility, in addition to high ascorbic (>68%) and phenolic (>55%) acid retention. Overall, we showed that spray-drying cashew juice is a feasible strategy to upcycle abundant and undervalued cashew juice into stable, phytochemical-rich ingredients for multiple applications. [ABSTRACT FROM AUTHOR]

Silica, as a stationary phase, has low separation efficiency accompanied by overlapping, broadened, and tailed peaks, so it needs to be modified to improve its efficiency. This study aims to develop a silica-based stationary phase modified by tetraethylene glycol (TEG) to separate phenolic compounds. Silica was modified by a chemical bond between silanol groups on the silica surface and TEG through a 3-glycidyloxypropylmethoxysilane reaction. The modified silica was packed into a capillary column and used to separate simple phenolic compounds consisting of phenol, pyrocatechol, and pyrogallol. A sample of 0.2 µL was injected into the capillary liquid chromatography and the mobile phase employed was acetonitrile 98% with a flow rate of 3 μL min−1. Elution was also done isocratically in this process and detection was carried out at a wavelength of 254 nm. The mixture of simple phenolic compounds was successfully separated in less than 7 min. The asymmetry factor and resolution were 1.43–2.12 and 1.72–5.43 respectively. The number of the theoretical plates ranged from 213 to 7,857. Columns containing Si-TEG stationary phase also separate phenolic compounds, which consist of gallic acid, syringic acid, ferulic acid, and caffeic acid. A sample of 0.2 µL was injected into the capillary liquid chromatography and successfully separated the mixture in less than 12 min. The samples were eluted isocratically using a mixture of methanol and 50 mM phosphate buffer pH 2.5 (8:92) with a flow rate of 3 μL min−1. The phenolic acids compounds were detected at a wavelength of 280 nm. The chromatogram showed four separate peaks. The asymmetry factor and resolution were 1.53–1.63 and 1.14–1.74, respectively, but the number of the theoretical plates was low, ranging from 190 to 796. [ABSTRACT FROM AUTHOR]

Highlights: Total phenolic contents and total flavonoid contents from Averrhoa bilimbi showed correlation to antioxidant properties. Freeze drying preserved the antioxidative properties of fruit compared to oven drying. Low moisture food product from A. bilimbi with ranging Aw (0.48 to 0.51) and moisture content (15.56 to 24.81%). A. bilimbi exhibited different microstructural and functional group after exposed to different drying method. The use of natural-based ingredients for nutricosmetics, boasting remarkable phytochemical compounds and antioxidant properties sourced from underutilized fruits, has also gained widespread recognition. The present study was conducted to preserve Averrhoa bilimbi using emerging technology of freeze drying. Low moisture food ingredient in powder form was produced from A. bilimbi fruit. The freeze dried of A. bilimbi was compared with classic drying method such as oven drying in term of moisture content, water activity, total phenolic content, total flavonoid content, DPPH, and ABTS scavenging activities. The result showed that freeze dried A. bilimbi exhibited highest total phenolic content (18.59 ± 3.39 µg GAE /mg), total flavonoid content (0.010 ± 0.000 µg RE /mg), the strongest DPPH (9.25 ± 0.43 mg/mL), ABTS (5.88 ± 0.26 mg/mL) scavenging activities, compared to oven drying technique. The moisture content of freeze dried A. bilimbi (24.81 ± 4.08%) exhibited higher compared to oven dried of A. bilimbi. The freeze dried A. bilimbi (0.48 ± 0.00 aw) demonstrated higher water activity compared to oven drying technique. Microstructure of freeze dried sample was different from oven dried sample. Oven dried sample microstructure experience a serious damage. The chemical screening and identification was conducted using gas chromatography (GC-MS) and high-performance liquid chromatography tandem mass spectrometry of triple quadrupole (HP LCMS-MS QTOF). The freeze drying technology presents a promising result of emerging technology for preserving fruit products. Hence, this emerging drying method presents a good scope for application in the food and cosmetic industry, with good commercial value. [ABSTRACT FROM AUTHOR]

Beneng taro leaves (Xanthosoma undipes K. Koch) are Banten's local biodiversity, which has recently been used as a substitute for tobacco in cigarettes. The availability of this commodity is abundant along with increased the planted area. Therefore, it is necessary to evaluate other potency such as functional activities and also their phytochemical profiles which have not reported yet. One of importance factors that influencing functional and phytochemical profiles is the polarity of extraction solvent. The purpose of this study was to analyze the chemical profile and free radical scavenging activity of beneng taro leaves extracted with various polarities solvent. The leaves extraction was carried out using non-polar, semi-polar and polar solvents, namely hexane, chloroform, acetone and water. Their phytochemical profile was conducted by analyzing total phenolic content and FTIR spectrum, while functional activities by determining free radical scavenging activity. The results showed that the highest phenolic content was obtained from chloroform extract, while the free radical scavenging activity (IC50) was acquired from acetone extract with values of 11.24 mg GAE/g and 36.83 µg/ml, respectively. The FTIR spectrum of the hexane, chloroform, and acetone extract of beneng taro leaves had similar profiles, while the water extract was different.

This study aims to produce a low-calorie ice cream with a higher nutritional value using red beetroot (Beta vulgaris L.). Four kinds of ice cream containing 5%, 10 % and 15% sucrose and single red beetroot (RB) ratio 15% and control (15% sucrose+ RB free) encoded as RB5, RB10, RB15 and C were produced respectively. The addition of RB increased pH and decreased the acidity (LA) and dry matter contents compared to control. Ash content was decreased depending on the sucrose ratio. While the lowest overrun value was determined in the RB10 sample (23.08%), the highest was in sample C (39.18%). The increasing of sucrose ratio generally increased the first dropping time and decreased the total melting time. The addition of sucrose reduced the viscosity and the highest viscosity values were observed in the sample RB5. While the lowest total phenolic content (TPC) was observed in the control sample (329.69 GAE mg/kg), the highest TPC was found in the RB5 sample (558.55 GAE mg/kg). Also, the lowest DPPH value was determined in the control sample (13.66% inhibition), the highest DPPH value was determined in the RB10 sample (27.98% inhibition). While fructose and glucose values decreased, sucrose values increased in the ice cream samples depending on the increasing of sucrose ratio.

Red raspberries are among the most significant wild edible fruits (WEFs) in Türkiye, thriving in cold to warm temperate regions across the country. This study focuses on 11 wild red raspberry ecotypes sampled from the Northern Anatolia region, examining their sensory, biochemical and antioxidant properties. Sensory analysis was used to compare ecotypes based on aroma, taste and juiciness. Biochemical assessments included soluble solid content (SSC), vitamin C amount, organic acids, total anthocyanins (TA), total phenolic content (TPC), total flavonoid content and total antioxidant capacity. The Ferric Reducing Antioxidant Power (FRAP) assay measured antioxidant capacity. Despite similar growing conditions, significant variations were observed among ecotypes and across years. In the first year, fruit weights ranged from 1.04 g to 1.33 g, and in the second year, they ranged from 0.97 g to 1.27 g. Fruit chroma values ranged between 26.11 and 33.70 in 2021, and 23.17 and 30.19 in 2022. Vitamin C exhibited considerable variability, ranging from 29.3 mg · 100 g−1 to 44.4 mg · 100 g−1 across ecotypes and years. TPC, total anthocyanin content (TAC) and total flavonoid content ranged from 164 mg to 390 mg gallic acid equivalent (GAE) · 100 g−1, 17.3 mg to 33.2 mg cyanidin-3-glucoside equivalent · 100 g−1 and 10.3 mg to 17.6 mg quercetin equivalent (QE) · 100 g−1, respectively, in both years. Citric acid emerged as the dominant organic acid across all ecotypes. Notably, the ecotypes V-4, V-8, V-3 and V-10 showcased larger, more appealing fruits suitable for fresh consumption, whereas V-3 and V-5 presented sweeter fruits ideal for processing. Additionally, the ecotypes V-6, V-7 and V-11 displayed higher levels of health-promoting compounds, such as TPC and antioxidant capacity, suggesting their potential as functional foods and valuable sources of natural antioxidants in the future.

The present study focuses on qualitative and quantitative phytochemical and biological analysis in bark and leaf extracts of Schima wallichii growing in Gulmi, Nepal. TPC and TFC were measured using the Folin-Ciocalteu reagent and the aluminum chloride colorimetric method, respectively. The DPPH assay was used for antioxidant activity, and antimicrobial activity was studied with the agar-well diffusion method. The presence of polyphenols, flavonoids, quinones, coumarins, saponins, terpenoids, and tannins was detected by qualitative phytochemical analysis. Total phenolic content in an aqueous leaf extract and methanol bark extract ranged from 199 ± 5 to 219 ± 8 mg GAE/g. Similarly, the flavonoid content found between 6.0 ± 1.7 in bark to 33 ± 2 mg QE/g in leaf extract. The antioxidant activity of IC50 36 ± 3, 39 ± 1, 52 ± 3, and 73 ± 4 µg/mL for methanolic bark extract, methanolic leaf, aqueous bark, and aqueous leaf extracts, respectively. The bark methanol extract showed the most antibacterial activity with ZOI of 10.0 ± 0.3, 8.0 ± 0.3, 14 ± 0, and 11 ± 1 mm against Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, respectively. Plant extracts were found to be nontoxic to mildly toxic to the brine shrimp nauplii. The plant is found to be rich in phenolics, and flavonoids showing significant antioxidant and antibacterial activities, and is nontoxic against brine shrimp nauplii. The plant imparts a health-promoting effect that needs to be explored in pharmaceutical research.

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Although legumes can meet nitrogen requirements via symbiotic nitrogen fixation, they must acquire phosphorus from the rhizosphere through their roots. Additionally, the fixation of phosphorus with various cations in soils reduces its availability, thereby decreasing its effectiveness and increasing production costs. This study was conducted during the 2021-22 and 2022-23 growing seasons to investigate the effects of seed pre-treatment (priming) and phosphorus doses on plant growth, yield attributes, and seed chemical composition in lentil at Siirt, Türkiye. Six seed priming treatments and four phosphorus doses were used in the study. The research was laid out in split-plot randomized complete block design with four replications. According to the results, phosphorus fertilizer increased seed yield, but there was no statistical difference between 15 and 60 kg P2O5 ha− 1. Seed priming had a synergistic effect, allowing for higher seed yield when combined with phosphorus fertilizer, especially silicon priming resulting in high seed yield even at low phosphorus fertilizer doses. This result also indicated that seed priming improved phosphorus efficiency. The highest seed yield and net income were obtained by 6 kg P2O5 ha− 1 with silicon priming treatment. Moreover, seed priming with salicylic acid, beneficial bacteria and silicon boosted growth and yield attributes, and seed chemical composition. Silicon priming increased the total antioxidant content in seeds while salicylic acid priming provided opportunities for both the increase of total antioxidants and phenolics. In conclusion, seed priming is an easy-to-implement and economical method for reducing phosphorus fertilizer and an effective way for higher profitability in lentil cultivation. [ABSTRACT FROM AUTHOR]

The present study focuses on qualitative and quantitative phytochemical and biological analysis in bark and leaf extracts of Schima wallichii growing in Gulmi, Nepal. TPC and TFC were measured using the Folin-Ciocalteu reagent and the aluminum chloride colorimetric method, respectively. The DPPH assay was used for antioxidant activity, and antimicrobial activity was studied with the agar-well diffusion method. The presence of polyphenols, flavonoids, quinones, coumarins, saponins, terpenoids, and tannins was detected by qualitative phytochemical analysis. Total phenolic content in an aqueous leaf extract and methanol bark extract ranged from 199 ± 5 to 219 ± 8 mg GAE/g. Similarly, the flavonoid content found between 6.0 ± 1.7 in bark to 33 ± 2 mg QE/g in leaf extract. The antioxidant activity of IC50 36 ± 3, 39 ± 1, 52 ± 3, and 73 ± 4 µg/mL for methanolic bark extract, methanolic leaf, aqueous bark, and aqueous leaf extracts, respectively. The bark methanol extract showed the most antibacterial activity with ZOI of 10.0 ± 0.3, 8.0 ± 0.3, 14 ± 0, and 11 ± 1 mm against Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, respectively. Plant extracts were found to be nontoxic to mildly toxic to the brine shrimp nauplii. The plant is found to be rich in phenolics, and flavonoids showing significant antioxidant and antibacterial activities, and is nontoxic against brine shrimp nauplii. The plant imparts a health-promoting effect that needs to be explored in pharmaceutical research. [ABSTRACT FROM AUTHOR]

Red apricot (Prunus spp.) and Black Fig (Ficus carica L.) are two of the most commonly grown summer fruits in the Nakhchivan region. In this preliminary study, ethanol fruit extracts were compared based on their total phenolic content (TPC) and antioxidant properties. According to the Folin-Ciocalteu method, the TPC was found to be 176.20 mg GAE/100 g in red apricot and 45.30 mg GAE/100 g in fig. Their antioxidant capacities were assessed using 2,2-Diphenyl-1- picrylhydrazyl (DPPH) radical scavenging activity. The scavenging activity, identified as the concentration of ethanol extract needed to scavenge half of the radicals (SC50), was determined to be 3.52 mg/mL for red apricot and 6.46 mg/mL for fig. Based on these two biological activity assays, red apricot exhibited higher phenolic composition and radical scavenging activity. However, further studies are required to understand whether these differences are attributed to secondary metabolites in these fruits. [ABSTRACT FROM AUTHOR]

Grapes are important sources of phenolic compounds, anthocyanins, and ascorbic acid, which are recognized for their significant biological benefits. This study aimed to explore the potential of using a nano zinc oxide coating to preserve the freshness and quality of grapes, as well as the properties of grape molasses. Nano zinc oxide was applied at three different concentrations (0.0%, 0.5%, and 1% w/v) through a three-stage spraying process during the flowering, unripe, and ripening stages, 20 days before harvest. Grape samples were then stored at 4 °C for 35 days. Throughout the storage period, various parameters were measured, including pH, titratable acidity, weight loss, Brix°, total phenolic content, anthocyanin and ascorbic acid levels, antioxidant activity, color indexes (L*, a*, b*), and microbial counts. Also, the properties of molasses were evaluated. Over time, there was an increase in pH (3.58–3.75 to 4.19–4.62), weight loss (0 to 7.12–10.2%), Brix° (22.44–22.69 to 25.95–28.62), color index b* (20.44–22.18 to 23.01–25.77), total viable count of bacteria (3.24–3.34 to 3.52–3.83 Log CFU/g), and total mold and yeast count (3.06–3.35 to 3.48–3.74 Log CFU/g), while titratable acidity (0.78–0.82 to 0.58–0.67%), phenolic content (1.56–1.88 to 1.29–1.43 mg/g), anthocyanin (0.04–0.05 to 0.03–0.04 mg/g) and ascorbic acid levels (10.03–12.53 to 6.66 to 9.10 mg of vitamin C/100 mL), antioxidant activity (30.06–35.6 to 26.23–29.13%), and color indexes L* (42.91–43.74 to 37.50–39.18) and a* (− 2.10 to − 3.30 to − 3.80 to − 5.36) decreased, along with a decline in sensory scores. By increasing the concentration of zinc oxide nanoparticles from 0 to 1% w/v, total sugar (66.85 to 67.56%) and Brix° (76.93 to 77.5) increased while conductivity (1.80 to 1.53 mS/cm) and specific gravity (1.55 to 1.38 g/cm3) decreased. The findings indicate that using a 0.5% w/v nano zinc oxide coating is effective in enhancing the freshness and maintaining the quality of grapes during storage. [ABSTRACT FROM AUTHOR]

Cutaneous melanoma is a complex pathology that still has only treatments that lack efficiency and offer many adverse effects. Due to this scenario emerges the need to analyze other possible treatments against this disease, such as the effect of phenolic compounds. These substances have proven antitumor effects, but still have not been fully explored as a form of therapy to combat melanoma. Also, the purinergic receptors, along with its system molecules, take part in the formation of tumors from many pathways, such as the actions of ectoenzymes and receptors activity, especially P2Rs family, and are formed by structures that can be modulated by the phenolic compounds. Therefore, more studies have to be made with the aim of explaining the purinergic system activity in carcinogenesis of cutaneous melanoma and the effects of its modulation by phenolic compound, in order to enable the development of new therapies to combat this aggressive and feared cancer. [ABSTRACT FROM AUTHOR]

The diverse nutrients found in sweet potato leaves, including vitamins, minerals, and antioxidants, offer a range of health benefits. The presence of carotenoids and polyphenols, both powerful health-promoting compounds, highlights their potential contribution to medical science. The main objective of this study was to ascertain essential functionals substances of antioxidant, phenolic compounds, and β-carotene in the leaves of 14 distinct sweet potato lines cultivated in open field. A comprehensive analysis of antioxidant capacity, phenolic content, and β-carotene was conducted using ABTS, Folin-Ciocalteu, and HPLC techniques, respectively. The study showed that the concentrations of total antioxidants, phenols, and β-carotene differed markedly among the leaf materials. Among the lines, SP-13 stands out with the highest concentration of phenols (124.64 mg/g dry weight), while SP-14 comes in at the opposite end with the lowest amount (62.97 mg/g dry weight) under field conditions. In the case of antioxidant content in line SP-3 showed the highest with 3.55 mg/g dry weight, while SP-14 brings up the lowest with 1.88 mg/g dry weight. Line SP-5 showed the most β-carotene (0.51 mg/g dry weight), while SP-11 had the least (0.05 mg/g dry weight). Therefore, it can be concluded that sweet potato leaves are a valuable dietary source of antioxidants, phenolic compounds, and β-carotene which have beneficial health elements.

Strawberry (Fragaria × ananassa Duch.) is one of the most widely consumed and cultivated fruits worldwide. Sorbitol plays a role in plant responses to many biotic and abiotic stresses. In this research, we intended to understand the effect of sorbitol spraying on the bioactive compounds of strawberry leaves. The application of sorbitol at different concentrations (0, 25, 50 mM and 75 mM) greatly improved strawberry characteristics such as total chlorophyll, chlorophyll a and b, carotenoids, and total phenolics. As sorbitol concentrations increased, chlorophyll a and chlorophyll b values increased in the samples taken during the fruiting period and higher values were obtained. The carotenoid content increased by approximately 189.49% and the total phenolic content increased by 30.85% in strawberry plants treated with sorbitol compared with the control. Supply of sorbitol decreased flavonoid content. The results indicate that sorbitol treatment has no inhibitory influence on the overall growth of strawberries. Among the biochemical parameters analyzed, chlorophyll, phenolic, and carotenoid contents increased, whereas flavonoid content decreased with sorbitol application.

Abstract Dayak tribes indigenous to the Indonesian island of Borneo has been using Bajakah Kalalawit (Uncaria gambir Roxb.) as traditional medicine for ages. This inspired us to develop phenolic from Bajakah Kalalawit extract as antibacterial agent. The extraction was done through decoction method and the determination of phenolic concentration was done using a visible spectrophotometer and Folin–Ciocalteu reagent (mixture of phosphotungstic and phosphomolybdic acids). We investigated the possibility of developing phenolic nanoparticle for future work. Kirby-Bauer method was used to assess antibacterial activity of phenolic against Staphylococcus aureus and the results were compared to Chloramphenicol in terms of its efficacy and duration of inhibition. This study contributes to the ongoing effort to address antibiotic resistance through the development of innovative antibacterial agents derived from natural sources. The results provide valuable insights into the potential of Bajakah Kalalawit phenolic extracts as a promising avenue for combating bacterial infections in the future.

Abstract Trifloxystrobin (TFS) is a widely used strobilurin class fungicide. Ginkgo biloba L. has gained popularity due to its recognized medicinal and antioxidant properties. The aim of this study was to determine whether Ginkgo biloba L. extract (Gbex) has a protective role against TFS-induced phytotoxicity, genotoxicity and oxidative damage in A. cepa. Different groups were formed from Allium cepa L. bulbs subjected to tap water (control), 200 mg/L Gbex (Gbex1), 400 mg/L Gbex (Gbex2), 0.8 g/L TFS solution (TFS), 200 mg/L Gbex + 0.8 g/L TFS (TFS + Gbex1) and 400 mg/L Gbex + 0.8 g/L TFS (TFS + Gbex2), respectively. The phenolic composition of Gbex and alterations in the morphological, physiological, biochemical, genotoxicity and anatomical parameters were evaluated. Rutin, protocatechuic acid, catechin, gallic acid, taxifolin, p-coumaric acid, caffeic acid, epicatechin, syringic acid and quercetin were the most prevalent phenolic substances in Gbex. Rooting percentage, root elongation, weight gain, chlorophyll a and chlorophyll b decreased by approximately 50%, 85%, 77%, 55% and 70%, respectively, as a result of TFS treatment compared to the control. In the TFS group, the mitotic index fell by 28% compared to the control group, but chromosomal abnormalities, micronuclei frequency and tail DNA percentage increased. Fragment, vagrant chromosome, sticky chromosome, uneven chromatin distribution, bridge, vacuole-containing nucleus, reverse polarization and irregular mitosis were the chromosomal abnormalities observed in the TFS group. The levels of proline (2.17-fold) and malondialdehyde (2.71-fold), as well as the activities of catalase (2.75-fold) and superoxide dismutase (2.03-fold) were increased by TFS in comparison to the control. TFS-provoked meristematic disorders were damaged epidermis and cortex cells, flattened cell nucleus and thickened cortex cell wall. Gbex combined with TFS relieved all these TFS-induced stress signs in a dose-dependent manner. This investigation showed that Gbex can play protective role in A. cepa against the phytotoxicity, genotoxicity and oxidative damage caused by TFS. The results demonstrated that Gbex had this antioxidant and antigenotoxic potential owing to its high phenolic content.

Because grape pomace powder (GPP) contains abundant phenolic chemicals and fibres, GPPs can serve as a filler in developing novel food products. This study examined how the GPP amounts affected a composite flour's physicochemical properties and bakery snacks' chemical, technical, and sensory properties. The experimental procedure involved replacing wheat flour (WF) with GPP at 5-20% while maintaining 100% WF as the control. The addition of GPP resulted in a significant decrease in the oil absorption capacity (P ≤ 0.05), while the rehydration index and water absorption capacity increased (P ≤ 0.05). The peak length (5.44-5.90 min), pasting temperature (70.20-80.92 °C), peak viscosity (124.72-172.80 RVU; RVU - relative value unit), trough viscosity (60.76-82.04 RVU), breakdown viscosity (69.56-93.74 RVU), final viscosity (162.70-222.30 RVU), and setback viscosity were measured. The addition of GPP to the composite flour and snacks decreased the lightness (L*) and increased the redness (a*) and yellowness (b*). The items' higher dietary fibre (DF) allowed them to claim 'high fibre content' when the maximum GPP was added. The GPP also increased the snacks' total solids, protein, ash, fibre, total phenolics content (TPC), and antioxidant capacity. The sensory acceptability of the snacks made with 5-10% GPP instead of WF was higher.

Bacteria are one of the organisms that may cause disease in the human body. Some bacteria which often infect the human body are Escherichia coli and Staphylococcus aureus. Both bacteria are pathogenic and possibly lead to various diseases such as diarrhea, urinary tract infections, and lung infections that have the potential to result in death. Treatment of infections caused by these bacteria can be done by giving antibiotics as therapy or a quick cure against infection. The use of antibiotics has side effects that result in resistance. Therefore, it is necessary to screen bioactive compounds with bioactivity as better antibacterial agents so that they can be developed into drugs that can cure diseases caused by bacteria. It is urgent to utilize natural antibacterials from plants to treat bacterial infections. This research aims to obtain the compound content, the total phenolic, and antibacterial activity of acetone extract of rambai leaves, so this research focused on rambai leaf acetone extract phytochemical content, total phenolic content, and antibacterial activity examination. Then, Phytochemical testing of rambai leaf acetone extract (Baccaurea motleyana) shows rambai leaves contain alkaloid compounds, tannins, flavonoids, saponins, steroids, and terpenoids that have the potential to be antibacterial. The examination of phenolic content was accomplished by using the Folin-Ciocalteau method. The total phenolic test results obtained were 68.63 mg EAG/g. Antibacterial testing was performed by implementing the disk diffusion method. Antibacterial testing of rambai leaf acetone extract Concentration of 20 %, 40%,60%, and 80% showed consecutive results in inhibition zones of 3.86 mm, 6.48 mm, 6.55 mm, and 6.63 mm with weak to medium activity for E. coli bacteria. However, S. aureus bacteria showed consecutive results in inhibition zones of 6.02 mm, 10.15 mm, 17.10 mm, and 19.46 mm with medium to intense activity. There has not been much research on rambai from Bangka Belitung, so there is an indispensable for further research regarding the total flavonoid and total alkaloid content of rambai leaves. Research may also carried out using a variety of solvents.

Abstract Black cumin seeds are extensively utilized for foods, cosmetics, and medicinal purposes. The current investigation was undertaken to analyzse the nutritional values, fatty acid compositions, bioactive compounds, and antioxidant roles of different black cumin genotypes available in Bangladesh. The genotype BSK-2074 had a higher content of proximate composition of crude oil (35.17%), crude protein (23.51%), total carbohydrate (35.23%), moisture (6.54%), ash (3.74%), crude fiber (8.12%), and gross energy value (644.88 kcal/100 g) than the released varieties and cultivars of black cumin available in Bangladesh. The major fatty acid profile of the seed-oil of the BSK-2074 genotype revealed that the oil contained a high amount of linoleic (59.39%), oleic (25.72%), palmitic (13.11%), and stearic (2.25%) acids, as well as a low amount of arachidic, linolenic, myristic, behenic, palmitoleic, lignoceric, and eicosadienoic acids. Moreover, the genotype BSK-2074 contained a high amount of unsaturated (UFAs, 85%), and low content of saturated (SFAs, 18%) fatty acids, and the ratio of UFAs to SFAs was higher (4.39–5.37). Again, the seeds of the genotype BSK-2074 were also enriched in different minerals, viz., calcium, magnesium, potassium, phosphorus, iron, and zinc. Moreover, total phenolic contents (TPC) and flavonoid contents (TFC) were found significantly higher in the genotype BSK-2074 (478.47 ± 7.33 mg GAE/100 g and 284.07 ± 2.08 mg QE/100 g, respectively) than the local Kalozira variety (316.48 ± 2.33 mg GAE/100 g and 120.53 ± 3.57 mg QE/100 g) found in Bangladesh. The activities of antioxidants 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals and ferric reducing antioxidant power (FRAP) were found significantly higher in BSK-2074 (37.93% and 129.65 ± 0.19 mg GAE/100 g), than the local Kalozira variety (98.09% and 193.75 ± 0.19 mg GAE/100 g). Thus, the new genotype, BSK-2074, was identified and suggested as the most promising and nutritionally enriched genotype of black cumin.

Background: Breast and esophageal cancer are the most aggressive and prominent causes of death worldwide. In addition, these cancers showed resistance to current chemotherapy regimens with limited success rates and fatal outcomes. Recently many studies reported the significant cytotoxic effects of phenolic and terpene fractions extracted from various Prunus species against different cancer cell lines. As a result, it has a good chance to be tested as a complement or replacement for standard chemotherapies. Methods: The study aimed to evaluate the cytotoxicity of phenolic and terpene fractions extracted from Iraqi Prunus arabica on breast (AMJ13) and esophageal (SK-GT-4) cancer cell lines by using the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide). Analysis using the Chou-Talalay method was performed to assess the synergistic effect between the extracted fractions and chemotherapeutic agent (docetaxel). Moreover, high-performance liquid chromatography (HPLC) analysis was conducted for the quantitative determination of different bioactive molecules of both phenolic and terpene fractions in the extract. Results: According to the findings, the treatment modalities significantly decreased cancer cell viability of AMJ13 and SK-GT-4 and had insignificant cytotoxicity on the normal cells (normal human fibroblast cell line) (all less than 50% cytotoxicity). Analysis with Chou-Talalay showed a strong synergism with docetaxel on both cancer cell lines (higher cytotoxicity even in low concentrations) and failed to induce cytotoxicity on the normal cells. Important flavonoid glycosides and terpenoids were detected by HPLC, in particularly, ferulic acid, catechin, chlorogenic acid, β-sitosterol, and campesterol. Conclusions: In conclusion, the extracted fractions selectively inhibited the proliferation of both cancer cell lines and showed minimal cytotoxicity on normal cells. These fractions could be naturally derived drugs for treating breast and esophageal cancers.

Dayak tribes indigenous to the Indonesian island of Borneo has been using Bajakah Kalalawit (Uncaria gambir Roxb.) as traditional medicine for ages. This inspired us to develop phenolic from Bajakah Kalalawit extract as antibacterial agent. The extraction was done through decoction method and the determination of phenolic concentration was done using a visible spectrophotometer and Folin–Ciocalteu reagent (mixture of phosphotungstic and phosphomolybdic acids). We investigated the possibility of developing phenolic nanoparticle for future work. Kirby-Bauer method was used to assess antibacterial activity of phenolic against Staphylococcus aureus and the results were compared to Chloramphenicol in terms of its efficacy and duration of inhibition. This study contributes to the ongoing effort to address antibiotic resistance through the development of innovative antibacterial agents derived from natural sources. The results provide valuable insights into the potential of Bajakah Kalalawit phenolic extracts as a promising avenue for combating bacterial infections in the future. [ABSTRACT FROM AUTHOR]

Response surface methodology was used to optimize the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) and total phenols (TP) from Berberis kongboensis fruit. Single-factor experiments were conducted with the ultrasonication power, liquid–solid ratio, ethanol concentration, extraction temperature, and extraction time. A Plackett–Burman design was used to identify the main factors affecting the extraction rates of TF and TP. A Box–Behnken design was used to further optimize the ethanol concentration, solvent/sample ratio, and extraction temperature. Under the optimum conditions, the ethanol concentration of 34.67%, solvent/sample ratio of 38.44 mL/g, and extraction temperature of 46.53 °C, the yields of TF and TP were 107.92 mg rutin equivalents/g and 4.86 mg gallic acid equivalents/g, respectively. The extract from UAE had a better yield and higher antioxidant activities in vitro than that obtained by conventional solvent extraction. The B. kongboensis fruit is rich in flavonoids and phenolics, and it has potential application value in the food industry. [ABSTRACT FROM AUTHOR]

Ajowan (Trachyspermum ammi L.) is considered a valuable spice and medicinal herb. In this study, the essential oil content and composition of the aerial parts of ajowan were investigated under different drying treatments (sun, shade, oven at 45 °C, oven at 65 °C, microwave, and freeze drying). Moreover, the phenolic content, flavonoid content, and antioxidant capacity of samples were also assessed. Fresh samples produced the highest essential oil content (1.05%), followed by those treated under sun (0.7%) and shade drying (0.95%). Based on gas chromatography–mass spectrometry (GC–MS), thirty compounds were determined in which thymol (34.84–83.1%), carvacrol (0.15–32.36%), p-cymene (0.09–13.66%), and γ-terpinene (3.12–22.58%) were the most abundant. Among the drying methods, freeze drying revealed the highest thymol content, followed by drying in a 45 °C oven. The highest TPC (total phenolic content) and TFC (total flavonoid content) were obtained in the fresh sample (38.23 mg TAE g−1 dry weight (DW)) and in the sample oven-dried at 45 °C (7.3 mg QE g−1 DW), respectively. Based on the HPLC results, caffeic acid (18.04–21.32 mg/100 gDW) and ferulic acid (13.102–19.436 mg/100 g DW) were the most abundant phenolic acids, while among flavonoids, rutin constituted the highest amount (10.26–19.88 mg/100 gDW). Overall, freeze drying was the most promising method of drying for preserving the phenolic (TPC) and flavonoid (TFC) compounds and oil components. [ABSTRACT FROM AUTHOR]

Sage (Salvia officinalis L.) is a medicinal and aromatic plant (MAP) belonging to the Lamiaceae family. Its morphological, productive and chemical characteristics are affected by abiotic and biotic factors. The use of biostimulants seems to be one of the most interesting innovative practices due to fact they can represent a promising approach for achieving sustainable and organic agriculture. Despite a large application in horticulture, the use of biostimulants on MAPs has been poorly investigated. On this basis, a field experiment in a 2-year study was done to assess the effect of foliar treatments with different types of biostimulants (containing seaweeds, fulvic acids and protein hydrolysates) and two frequencies of application on morphological, productive, and chemical characteristics of S. officinalis grown organically in Mediterranean environment. Morphological, productive, and chemical parameters were affected by the factors. The biostimulant application generated higher plant height, chlorophyll content, relative water content, biomass yield and essential oil yield compared to control plants. In addition, more frequent application of biostimulants produced higher biomass and essential oil yield. The application of fulvic acid and protein hydrolysates every week produced the highest total fresh yields (between 3.9 and 8.7 t ha-1) and total dry yields (between 1.3 and 2.5 t ha-1). The essential oil yield almost doubled (33.9 kg ha-1) with a higher frequency of protein hydrolysates application. In this study, 44 essential oil compounds were identified, and the frequency factor significantly influenced the percentage of 38 compounds. The highest percentage of some of the most representative monoterpenes, such as 1,8-cineole, a-thujone and camphor, were observed in biostimulated plants, with average increases between 6% and 35% compared to control plants. The highest values for total phenolics, rosmarinic acid, antioxidant activity were obtained in control plants and with a lower frequency of biostimulant applications. This study emphasizes how biostimulant applications may be used to improve sage production performance and essential oil parameters when produced in agricultural organic system. At the same time, biostimulants application caused a decrease in total phenolic, antioxidant activity and rosmarinic acid values. [ABSTRACT FROM AUTHOR]

Because grape pomace powder (GPP) contains abundant phenolic chemicals and fibres, GPPs can serve as a filler in developing novel food products. This study examined how the GPP amounts affected a composite flour's physicochemical properties and bakery snacks' chemical, technical, and sensory properties. The experimental procedure involved replacing wheat flour (WF) with GPP at 5-20% while maintaining 100% WF as the control. The addition of GPP resulted in a significant decrease in the oil absorption capacity (P = 0.05), while the rehydration index and water absorption capacity increased (P = 0.05). The peak length (5.44-5.90 min), pasting temperature (70.20-80.92 °C), peak viscosity (124.72-172.80 RVU; RVU - relative value unit), trough viscosity (60.76-82.04 RVU), breakdown viscosity (69.56-93.74 RVU), final viscosity (162.70-222.30 RVU), and setback viscosity were measured. The addition of GPP to the composite flour and snacks decreased the lightness (L*) and increased the redness (a*) and yellowness (b*). The items' higher dietary fibre (DF) allowed them to claim 'high fibre content' when the maximum GPP was added. The GPP also increased the snacks' total solids, protein, ash, fibre, total phenolics content (TPC), and antioxidant capacity. The sensory acceptability of the snacks made with 5-10% GPP instead of WF was higher. [ABSTRACT FROM AUTHOR]

Algae are increasingly becoming the center for interest due to researchers for their composition of various important bioactive molecules. The objective of this study is to investigate the seasonal variations in the concentrations of flavonoids, polyphenols, chlorophyll a, chlorophyll b, and carotenoids, as well as the antimicrobial activity of methanolic extracts from the red alga Corallina officinalis collected from western Algeria. The results of the biochemical assays show that the best polyphenol contents are recorded by the summer extract with a concentration of 33.29 µgEqAG/mg. The recorded flavonoid contents are 967.33; 869; 239.66 and 156 µgEQ/mg for summer, autumn, spring and winter respectively. For chlorophyll a and b the winter extract recorded the highest values. The best results of antibacterial tests were noted for the summer season with diameters of 14.33 mm against the two-bacteria E. coli and Staphylococcus aureus resistant to methicillin (SARM). The autumn extract gave low activity against E. coli, Pseudomonas, SARM and Klebsiella. For the winter extract we noted resistance against B. cereus, Pseudomonas and B. subtilis while E. coli, SARM, Klebsiella and the yeast Candida albicans recorded low activity. For the spring extract, resistance was observed against all the bacteria tested except for the E. coli and Klebsiella strains. The extracts obtained can be used as an antibacterial due to their rich content of bioactive molecules. The best results of antibacterial tests are noted for the summer season. [ABSTRACT FROM AUTHOR]

Snack foods have experienced substantial growth in consumption globally, appealing to various age groups and becoming a significant segment of the food industry. The current investigation aimed to assess the nutritional, antioxidant, and sensory characteristics of an unconventional pie prepared from brown wheat flour(BWF), carob bean flour (CBF), onion, scallions, and roasted seaweeds. Chemical composition, minerals content, functional properties, total phenolics, total flavonoids, and antioxidants activity (DPPH), and phenolic profile of CBF, BWF, and binary combinations were evaluated. Five pies using different formulas (CGR0, CGR1, CGR2, CGR3, CGR4, and CGR5) were developed, where CGR0 serving as the control sample with 100% BWF. The other formulations (CGR1–CGR5) are binary combinations of BWF and CBF at various ratios (90:10, 80:20, 70:30, 60:40, and 50:50%). The onion pie samples were assayed for proximate composition, minerals, and sensory properties. CBF is rich in dietary fibers, with a content of 12.73%, substantial ash (4.79%), and carbohydrates (74.36%). Blending BWF with CBF significantly enhances the functional properties of the mixture, as indicated by improved WAC, AOC, EA, and FC compared to BWF. The addition of CBF into BWF in produced pie recipes led to significant increases in Fat, fiber, and ash. Additionally, substituting BWF with CBF in pie samples led to substantial decreases in carbohydrate content. The highest content of micro- and macro-elements was found in CGR5 samples, whereas the lowest was found in control samples CGR0. The results found that incorporating CBF into onion pie samples at levels between 10% to 40% can positively impact the sensory acceptability of the produced pie samples. [ABSTRACT FROM AUTHOR]

Waxy corn (Zea mays var. ceratina) is a pigmented corn rich in anthocyanins. Numerous studies have proved various biological activities of anthocyanins, and one of them is antioxidants. Antioxidants will donate electrons to stop or reduce rampaging free radicals in target particles. Several methods have been used to improve pigmented waxy corn's anthocyanin content, and one of them is conventional breeding through the reciprocal cross. Thus, this research investigates the effects of reciprocal cross on the anthocyanin content and its antioxidant activity in waxy corn. The total anthocyanin content (TAC) was determined using the pH differential method. On the other hand, the total phenolic content (TPC) was analyzed by the Folin- Ciocalteu colorimetric method. Then, the antioxidant activity was assayed through 2,2-Diphenyl-2-hydrazyl (DPPH) and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. The results indicated that hybrid BL 1-7 x BL 1-8 had the highest TAC and TPC at 71.43 mg/100g and 7.04 mg of GAE/g, respectively. BL 1-7 x BL 1-8 had the lowest IC50 values which are 133.68 ug/mL for DPPH assay and 17.13 ug/mL for ABTS assay. It is an exciting finding from this study to utilize inbred BL 1-7 as maternal plant for enhancement of anthocyanin content and antioxidant activity. Furthermore, TAC and TPC were significantly correlated with DPPH and ABTS activity. These correlations helped to add more information on the relationship between phytochemicals and antioxidant activity. The information obtained from this study is useful for making smart selection of inbreds that will result in new waxy corn hybrids that are full of health advantages and provide functional foods like natural colorants. [ABSTRACT FROM AUTHOR]

Four new polyoxygenated cucurbitane triterpene glycosides named kedrojiftene A-D (4-7), were isolated via different chromatographic techniques along with three phenolic compounds (1-3) from the crude extract of Kedrostis gijef. The isolated compounds were elucidated based on 1D, 2D-NMR spectroscopic analyses, HRESIMS and comparison with the related metabolites in literature. In bioassay, the α-glucosidase inhibitory assay for the isolated compounds was carried out and compounds (1-3) showed significant inhibitory activities. [ABSTRACT FROM AUTHOR]

The chemical composition of Sedum ewersii Ledeb., a plant indigenous to Kazakhstan and traditionally utilized in folk medicine, was comprehensively investigated, with a focus on its various plant parts. Fresh samples collected in May 2023 from the Almaty region underwent hydrodistillation to extract volatile components, followed by analysis using gas chromatography coupled with mass spectrometric detection, which identified a total of 71 compounds across different plant parts, including the root (underground part), root (aerial part), leaf, stem, and flowering aerial part. The predominant biologically active compound identified across all plant parts was Ethyl α-D-glucopyranoside. Monoterpenes, recognized as primary secondary metabolites, were notably abundant in each plant part, with varying compositions: the root (underground part) contained 28.58% aliphatic monoterpenes, 54.41% oxygenated monoterpenoids, 1.42% diterpenoids, and 15.59% other compounds; the root (aerial part) exhibited 1.34% aliphatic monoterpenes, 31.28% oxygenated monoterpenoids, 6.16% diterpenoids, and 61.22% other compounds; the stem and leaves showed 3.06% aliphatic monoterpenes, 21.49% oxygenated monoterpenoids, 17.99% diterpenoids, and 57.46% other compounds; and the flowering aerial part displayed 8.20% aliphatic monoterpenes, 53.18% oxygenated monoterpenoids, 23.75% diterpenoids, and 14.87% other compounds. Diterpenes, particularly Phytol, were prominently present in the leaf, stem, and flowering aerial parts. Additionally, a diverse array of organic acids, ketones, and phenolic compounds were identified across the plant parts, each potentially offering distinct pharmacological benefits. The presence of exclusive compounds in specific plant parts, such as Dihydroxyacetone in the root (aerial part), underscored the pharmacological diversity of S. ewersii. This study provides valuable insights into the chemical diversity and pharmacological potential of S. ewersii, suggesting promising applications in pharmaceutical and medicinal fields. Further research aimed at elucidating the individual and synergistic pharmacological effects of these compounds is crucial to fully harness the therapeutic benefits of this plant. [ABSTRACT FROM AUTHOR]

Sandalwood essential oil, known for its rich content of phenolic and flavonoid compounds, holds great promise for applications in perfumery and medicine. However, traditional production methods have raised concerns regarding their environmental impact and sustainability. This study explored subcritical ethanol extraction as a green technique to address these concerns. Under optimized conditions (6.04 MPa, 109 °C, and 2.01 mL/min), maximum oil yield reached 4.04% with substantial total phenolic compounds (TPCs) of 4.11 mg GAE/100 g and total flavonoid compounds (TFCs) of 8.85 mg QE/100 g in the extracted sandalwood oil. Furthermore, the oil displayed notable antioxidant activity AA of 71.68%. The temperature was identified as a significant factor affecting oil yield, TPCs, TFCs, and AA. The fine-tuning of the extraction temperature enhanced the desired characteristics, improving bioactive compound yields and heightening antioxidant potential. This study uses a green extraction technique to contribute to sustainable sandalwood essential oil production. [ABSTRACT FROM AUTHOR]

With climate warming and economic globalization, insect–microbe assemblages are becoming increasingly responsible for various devastating forest diseases worldwide. Japanese larch (Larix kaempferi) is extensively cultivated in China because of its high survival rate, rapid maturation and robust mechanical properties. Endoconidiophora fujiensis , an ophiostomatoid fungus associated with Ips subelongatus , has been identified as a lethal pathogen of L. kaempferi in Japan. However, there is a dearth of research on the pathogenicity of E. fujiensis in larches in China. Therefore, we investigated the pathogenicity of E. fujiensis in introduced L. kaempferi and indigenous larch (Larix olgensis) trees and compared the induced resistance responses to the pathogen in both tree species in terms of physiology and gene expression. Five-year-old saplings and 25-year-old adult trees of L. olgensis and L. kaempferi were inoculated in parallel during the same growing season. Endoconidiophora fujiensis exhibited high pathogenicity in both larch species, but particularly in L. kaempferi compared with L. olgensis adult trees; adult L. olgensis was more resistant to E. fujiensis than adult L. kaempferi , which was reflected in higher accumulation of defensive monoterpenes, such as myrcene, 3-carene and limonene and the earlier induction of defense genes catalase (CAT) and pathogenesis-related protein 1 (PR1). This study contributes to our understanding of the interactions between bark beetle-associated ophiostomatoid fungi and host larches, from phenotypic responses to alterations in secondary metabolites via defense- and metabolism-related gene activation, providing a valuable foundation for the management of larch diseases and pests in the future. [ABSTRACT FROM AUTHOR]

In this study, propolis referred to as of Türkiye Anatolian propolis was utilized. This work was aim to prepare a 70% ethanolic extract of propolis and subsequently determine certain biochemical parameters, total phenolic content (TPC), total flavonoid content (TFC), phenolic and flavonoid composition, inhibitory effects on certain enzymes [acetylcholinesterase (AChE), urease, and alpha-amylase], along with assessments of antiviral activity against Vero cells, lung cancer cells (A549), and breast cancer cells (MDA-MB-231). The richness of phenolic content in Anatolian propolis clearly indicates a high inhibitory effect on these enzymes. Additionally, it was observed to exert significant effects on cancer cells and demonstrated effective antiviral activity. It can be contemplated that the Anatolian propolis might serve as a potential source for novel drugs, and the isolation of its compounds could offer opportunities for utilization in traditional medicine. [ABSTRACT FROM AUTHOR]

In this study, vinegars were produced using traditional method from 15 different fruits (rosehip, pear, fig, wild pear, apple, plum, hawthorn (yellow and red), pomegranate, grape (cimin, cardinal), peach, cranberry, quince and medlar). It was aimed to determine the total phenolic, total flavonoid and total monomeric anthocyanin contents and antioxidant capacities of vinegars. As a result, it was concluded that the phenolic, flavonoid contents and the antioxidant capacity in all 3 methods (TEAC, FRAP and DPPH) of rosehip vinegar was significantly higher than other vinegars. In addition, wild pear and hawthorn vinegars were very rich in phenolic and flavonoid compounds and these vinegars had very high antioxidant capacity. The results of the study suggest that an alternative consumption method can be provided by producing vinegar, which is a healthy, aromatic and alternative product with high added value, from fruits such as rosehip, wild pear, hawthorn, cranberry, quince and medlar, whose usage and consumption are limited due to their natural structure. [ABSTRACT FROM AUTHOR]

Background: This research aims to explore the phenolics identification, phenolics quantification, antioxidant and potential biofunctional properties of lesser-known Thai fruits and their potency to treat type 2 diabetes mellitus (T2DM). Including, Antidesma puncticulatum, Dillenia indica, Diospyros decandra, Elaeagnus latifolia, Flacourtia indica, Garcinia dulcis, Lepisanthes fruticose, Mimusops elengi, Muntingia calabura, Phyllanthus reticulatus, Streblus asper, Syzygium cumini, Syzygium malaccense, Willughbeia edulis and Schleichera oleosa were analyzed by their phenolic and flavonoid content. These fruits have received limited scientific attention, prompting an investigation into their health benefits, particularly their relevance to diabetes management. Methods: The study utilized methanolic crude extracts to measure phenolic and flavonoid levels. Additionally, UHPLC-DAD was utilized to identify and quantify phenolics. The methanolic extracts were assessed for antioxidant and antidiabetic abilities, including α-glucosidase and α-amylase inhibition. Results and Conclusion: The study highlighted S. cumini as a rich source of phenolic (980.42 ± 0.89 mg GAE/g and flavonoid (3.55 ± 0.02 mg QE/g) compounds with strong antioxidant activity (IC50 by DPPH; 3.00 ± 0.01 µg/ml, IC50 by ABTS; 40 ± 0.01 µg/ml, FRAP; 898.63 ± 0.02 mg TE/ml). Additionally, S. cumini exhibited promising antidiabetic effects (S. cumini IC50; 0.13 ± 0.01 mg/ml for α-glucosidase inhibition, 3.91 ± 0.05 mg/ml for α-amylase inhibition), compared to Acarbose (IC50; 0.86 ± 0.01 mg/ml for α-glucosidase inhibition, 0.39 ± 0.05 mg/ml for α-amylase inhibition). Remarkably, compounds like catechins, gallic acid, kaempferol, and ellagic acid were identified in various quantities.This study suggests that these fruits, packed with phenolics, hold the potential to be included in an anti-diabetic diet and even pharmaceutical applications due to their health-promoting properties. [ABSTRACT FROM AUTHOR]