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The aim of this study was to investigate the antioxidant and antimicrobial effect of two selected plants: Tussilago farfara L. and Cetraria islandica L. The antioxidant activity was evaluated by DPPH and phosphomolybdenum method, total polyphenol content with Folin – Ciocalteu reagent, flavonoids content by aluminium chloride method. The detection of antimicrobial activity was carried out by disc diffusion method and evaluation of minimal inhibition concentration against five species of Gram-negative bacteria: Escherichia coli CCM 3988, Klebsiella pneumoniae CCM 2318, Salmonella enterica subsp. enterica CCM 3807, Shigella sonei CCM 1373, Yersinia enterocolitica CCM 5671 and five Gram-positive bacteria: Bacillus thuringiensis CCM 19, Clostridium perfringens CCM 4435, Haemophilus influenzae CCM4456, Listeria monocytogenes CCM 4699, Stapylococcus aureus subsp. aureus CCM 2461. Tussilago farfara showed stronger antioxidant activity (DPPH method – 7.67 mg TEAC – Trolox equivalent antioxidant capacity per g of sample; phosphomolybdenum method – 111.67 mg TEAC per g of sample), total polyphenol (11.92 mg GAE – galic acid equivalent per g of sample) and flavonid content (47.62 μg QE – quercetin equivalent per g of sample) with compare to Centraria islandica. Tussilago farfara also showed the higher antimicrobial activity against Gram-negative bacteria confirmed by both methods – disc diffusion and minimal inhibitory concentration.