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4 results on '"rhopalosiphum-padi virus"'

1. The 5' non-translated region of Varroa destructor virus 1 (genus Iflavirus): structure prediction and IRES activity in Lymantria dispar cells

Author

Juliette R. Ongus, Els C. Roode, Monique M. van Oers, Cornelis W. A. Pleij, and Just M. Vlak

Subjects
Insecta, Genes, Viral, Molecular Sequence Data, Laboratory of Virology, picorna-like-virus, Picornaviridae, Spodoptera, Regulatory Sequences, Nucleic Acid, Transfection, cricket-paralysis-virus, hepatitis-c virus, internal ribosome entry, translation initiation, Laboratorium voor Virologie, Species Specificity, Genes, Reporter, Luciferases, Firefly, Virology, Lymantria dispar, Animals, Luciferase, RNA, Messenger, Cricket paralysis virus, Cells, Cultured, Rhopalosiphum padi virus, Reporter gene, Base Composition, biology, Base Sequence, Schneider 2 cells, nucleotide-sequence analysis, fungi, triatoma-virus, biology.organism_classification, Blotting, Northern, PE&RC, Internal ribosome entry site, Gene Expression Regulation, Nucleic Acid Conformation, RNA, Viral, insect, 5' Untranslated Regions, rhopalosiphum-padi virus, Ribosomes, Algorithms, rna viruses
Abstract

Structure prediction of the 5′ non-translated region (NTR) of four iflavirus RNAs revealed two types of potential internal ribosome entry site (IRES), which are discriminated by size and level of complexity, in this group of viruses. In contrast to the intergenic IRES of dicistroviruses, the potential 5′ IRES structures of iflaviruses do not have pseudoknots. To test the activity of one of these, a bicistronic construct was made in which the 5′ NTR ofVarroa destructorvirus 1 (VDV-1) containing a putative IRES was cloned in between two reporter genes, enhanced green fluorescent protein and firefly luciferase (Fluc). The presence of the 5′ NTR of VDV-1 greatly enhanced the expression levels of the second reporter gene (Fluc) inLymantria disparLd652Y cells. The 5′ NTR was active in a host-specific manner, as it showed lower activity inSpodoptera frugiperdaSf21 cells and no activity inDrosophila melanogasterS2 cells.

Published
2006
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2. Standard methods for virus research in Apis mellifera

Author

Jozef J. M. van der Steen, Dirk C. de Graaf, Brenda V. Ball, Philippe Blanchard, Yanping Chen, Joachim R. de Miranda, Magali Ribière, Giles E. Budge, Lina De Smet, Eugene V. Ryabov, Laurent Gauthier, Nor Chejanovsky, Lesley Bailey, and Elke Genersch

Subjects
viruses, Primary level, reverse-transcriptase pcr, messenger-rna quantification, Virus, Deformed wing virus, quantitative rt-pcr, real-time pcr, Rhopalosiphum padi virus, queen-cell-virus, biology, Host (biology), Transmission (medicine), Entomology & Disease Management, Honey bee, Standard methods, biology.organism_classification, Virology, picorna-like virus, 3. Good health, deformed-wing-virus, Evolutionary biology, Insect Science, adult honey-bees, rhopalosiphum-padi virus, bee-paralysis-virus
Abstract

Honey bee virus research is an enormously broad area, ranging from subcellular molecular biology through physiology and behaviour, to individual and colony-level symptoms, transmission and epidemiology. The research methods used in virology are therefore equally diverse. This article covers those methods that are very particular to virological research in bees, with numerous cross-referrals to other BEEBOOK papers on more general methods, used in virology as well as other research. At the root of these methods is the realization that viruses at their most primary level inhabit a molecular, subcellular world, which they manipulate and interact with, to produce all higher order phenomena associated with virus infection and disease. Secondly, that viruses operate in an exponential world, while the host operates in a linear world and that much of the understanding and management of viruses hinges on reconciling these fundamental mathematical differences between virus and host. The article concentrates heavily on virus propagation and methods for detection, with minor excursions into surveying, sampling management and background information on the many viruses found in bees.

Published
2013

3. Standard methods for virus research in Apis mellifera

Author

Miranda, J.R., Bailey, L., Ball, B.V., Blanchard, P., Budge, G.E., Chejanovsky, N., Chen, Y.P., Gauthier, L., Genersch, E., de Graaf, D.C., Ribiere, M., Ryabov, E., de Smet, L., van der Steen, J.J.M., Miranda, J.R., Bailey, L., Ball, B.V., Blanchard, P., Budge, G.E., Chejanovsky, N., Chen, Y.P., Gauthier, L., Genersch, E., de Graaf, D.C., Ribiere, M., Ryabov, E., de Smet, L., and van der Steen, J.J.M.

Abstract

Honey bee virus research is an enormously broad area, ranging from subcellular molecular biology through physiology and behaviour, to individual and colony-level symptoms, transmission and epidemiology. The research methods used in virology are therefore equally diverse. This article covers those methods that are very particular to virological research in bees, with numerous cross-referrals to other BEEBOOK papers on more general methods, used in virology as well as other research. At the root of these methods is the realization that viruses at their most primary level inhabit a molecular, subcellular world, which they manipulate and interact with, to produce all higher order phenomena associated with virus infection and disease. Secondly, that viruses operate in an exponential world, while the host operates in a linear world and that much of the understanding and management of viruses hinges on reconciling these fundamental mathematical differences between virus and host. The article concentrates heavily on virus propagation and methods for detection, with minor excursions into surveying, sampling management and background information on the many viruses found in bees.

Published
2013

4. The 5' non-translated region of Varroa destructor virus 1 (genus Iflavirus): structure prediction and IRES activity in Lymantria dispar cells

Author

Ongus, J.R., Roode, E.C., Pleij, C.W.A., Vlak, J.M., van Oers, M.M., Ongus, J.R., Roode, E.C., Pleij, C.W.A., Vlak, J.M., and van Oers, M.M.

Abstract

Structure prediction of the 5' non-translated region (NTR) of four iflavirus RNAs revealed two types of potential internal ribosome entry site (IRES), which are discriminated by size and level of complexity, in this group of viruses. In contrast to the intergenic IRES of dicistroviruses, the potential 5' IRES structures of iflaviruses do not have pseudoknots. To test the activity of one of these, a bicistronic construct was made in which the 5' NTR of Varroa destructor virus 1 (VDV-1) containing a putative IRES was cloned in between two reporter genes, enhanced green fluorescent protein and firefly luciferase (Fluc). The presence of the 5' NTR of VDV-1 greatly enhanced the expression levels of the second reporter gene (Fluc) in Lymantria dispar Ld652Y cells. The 5' NTR was active in a host-specific manner, as it showed lower activity in Spodoptera frugiperda Sf21 cells and no activity in Drosophila melanogaster S2 cells

Published
2006

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