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Large‐scale ecological restoration is a crucial tool for improving the quality and extent of habitat for wildlife. In Australia, 25,000 hectares of land is to be restored to support habitat connectivity for the Endangered koala (Phascolarctos cinereus). However, limited funding is a barrier to achieving restoration of this scale without the integration of new technologies and approaches. Seed enhancement technologies (SETs) are commonly used in agriculture to improve plant establishment and growth while reducing seed wastage; yet SETs are rarely used in restoration. Here, we tested the efficacy of SETs with five native tree species favored by koalas. Using a greenhouse trial, we measured emergence, time to emergence, growth rates, and survival up to 7 weeks post planting. At 33 weeks post‐planting, we measured total biomass, root: shoot ratios, and the specific root length. SETs resulted in comparable emergence and survival and promoted a longer time to emergence relative to bare seeds. Importantly, seedlings grown from SETs yielded higher average growth rates (0.3 mm/week higher), total biomass (5.93 mg higher) and root: shoot ratios (0.258 higher) relative to seedlings grown without SETs. These findings underscore the applicability of SETs to native species to support large‐scale koala habitat restoration by increasing the growth rate and size of seedlings at the early life stage. [ABSTRACT FROM AUTHOR]

Summary: The high cost of native seeds and low seedling establishment percentages after seed broadcasting are major constraints for seed‐based rehabilitation. This study examines whether extruded seed pelleting can be used as a tool to distribute seeds more effectively (e.g. distribute small seeds in a precise manner) while maintaining or enhancing seedling emergence and establishment of Australian shrub species used in the rehabilitation of Phytophthora cinnamomi infested Jarrah (Eucalyptus marginata) forest sites. Pellet formulation was first tested using five different formulations of dry ingredients and the addition of seeds from four Phytophthora‐resistant species under glasshouse conditions. The best performing formulation from this work was then adopted to compare laboratory‐produced and field‐deployed pellets. Seedling emergence of the larger seeded species, Raspberry Jam Wattle (Acacia acuminata) and Pin‐cushion Hakea (Hakea laurina), was highest using field‐deployed pellets and emergence was statistically similar to the non‐pelleted seeds. Seedling emergence reached 100% for the small seeded species, Silky‐leaved Bold Flower (Calothamnus sanguineus) and Melaleuca seriata, from laboratory‐produced pellets and was significantly higher than non‐pelleted seeds. These results indicate that extruded pelleting offers an alternative direct seeding option that can result in greater or equal seedling emergence than that observed with non‐pelleted seeds, yet the responses are species‐specific. These species‐specific pelleting techniques must now be tested under field conditions to confirm whether pellet production method and delivery improve seedling establishment under field conditions. [ABSTRACT FROM AUTHOR]

Abstract Large‐scale ecological restoration is a crucial tool for improving the quality and extent of habitat for wildlife. In Australia, 25,000 hectares of land is to be restored to support habitat connectivity for the Endangered koala (Phascolarctos cinereus). However, limited funding is a barrier to achieving restoration of this scale without the integration of new technologies and approaches. Seed enhancement technologies (SETs) are commonly used in agriculture to improve plant establishment and growth while reducing seed wastage; yet SETs are rarely used in restoration. Here, we tested the efficacy of SETs with five native tree species favored by koalas. Using a greenhouse trial, we measured emergence, time to emergence, growth rates, and survival up to 7 weeks post planting. At 33 weeks post‐planting, we measured total biomass, root: shoot ratios, and the specific root length. SETs resulted in comparable emergence and survival and promoted a longer time to emergence relative to bare seeds. Importantly, seedlings grown from SETs yielded higher average growth rates (0.3 mm/week higher), total biomass (5.93 mg higher) and root: shoot ratios (0.258 higher) relative to seedlings grown without SETs. These findings underscore the applicability of SETs to native species to support large‐scale koala habitat restoration by increasing the growth rate and size of seedlings at the early life stage.

Introduction: Safflower seeds are rich in unsaturated fatty acids with a high capacity for peroxidation, which have a high potential to reduce germination and seed vigor during the storage period. Therefore, Introducing appropriate methods to preserve or improve their germplasm during storage would be advantageous. The aim of this study was to investigate the effects of seed priming on germination and vigor of safflower seeds (Sofeh and Sina cultivars). Materials and Methods: A three-factor experiment was conducted in a completely randomized design with three replications before and after artificial deterioration. The experimental factors included controlled deterioration of seeds at 45°C in six levels (no deterioration, 1, 2, 3, 4, and 6 days) and priming in four levels (no prime, hydropriming, salicylic acid 50 mg/l and sodium chloride 5 percent). Results: Artificial aging strongly and linearly reduced the germination ability of safflower seeds, and germinability and seed vigor reach zero in a time interval which lasts between 2.5 to 4.5 days (depending on the treatment and the investigated trait). The use of priming prior to artificial aging was more advantageous than priming after artificial aging. In addition, priming with salicylic acid was more useful compared to other priming treatments. Conclusion: Priming of safflower seeds before storage would result in the extended shelf-life of the stored seeds while also preserving the seed germination potential. [ABSTRACT FROM AUTHOR]

Worldwide, there is a strong need for new, innovative, large‐scale approaches to restoring diverse native vegetation. Seed‐based revegetation, while often employed, can suffer from low plant establishment. Various seed coating technologies have been widely employed for decades to promote efficient agricultural planting; however, a significant obstacle to the implementation of this technology for native species revegetation is the limited access to the expertise and techniques which are mostly confidential to and specialized for the agrochemical industry. Here we investigated whether the seeds of Australian native species may benefit from extruded pelleting, measuring both seedling emergence and early growth, and testing the pellets with and without the addition of a commercial plant probiotic. A manipulative glasshouse experiment was carried out with three treatments (bare seeded; standard pellets; pellets amended with a probiotic) for four native plant species. Incorporation of seeds within standard pellets was shown to maintain high percentages of emergence and improve growth of three of the tested species (Daviesia ulicifolia, Hardenbergia violacea, and Indigofera australis) in the glasshouse setting, with increases in mean biomass of 83, 385, and 1,002%, respectively. However, emergence in the fourth species (Bursaria spinosa) was low in all seed treatments, perhaps due to excessive sowing depth (bare seeds), while combined with being encased inside a pellet. Relative to the standard pellets, probiotic amended pellets did not impart further detectable emergence or growth benefits. Ultimately, these results highlight exciting prospects for the application of extruded seed pellets in facilitating efficient use of seed in the revegetation of some native species. [ABSTRACT FROM AUTHOR]

Manganese is an important essential micronutrient, and its deficiency causes latent health issues in humans. Agronomic biofortification can promisingly improve the plant nutrient concentration without changing the genetic makeup of plants. This study was designed to assess the best method of Mn application to enhance productivity and grain Mn contents under conventional tillage (CT) and no tillage (NT) systems. Manganese was delivered through seed coating (250-mg kg−1 seed), osmopriming (0.1-M Mn solution), soil application (1 kg ha−1), and foliar application (0.25-M Mn solution). A general control with no seed Mn application was included, whereas hydropriming and water spray were used as positive control treatments for Mn seed priming and Mn foliar spray, respectively. No tillage had a higher total soil porosity (9%), soil organic carbon (16%), soil microbial biomass carbon (4%), nitrogen (2%), and soil nutrients in the CT system. Manganese nutrition through various methods significantly enhanced the yield, grain biofortification, and net benefits for CT and NT systems. Averaged across two years, the maximum improvement in grain productivity was recorded with osmopriming (28%) followed by foliar application (26%). The highest grain Mn concentration (29% over no application) was recorded with Mn foliar applications under both tillage systems. Moreover, the highest economic returns and marginal net benefits were recorded with osmopriming. To improve the wheat production, profitability, and grain Mn concentration, Mn application through priming and foliar application may be opted.

Zinc (Zn) deficiency is a global concern for human health and causes a decrease in crop production and nutritional characteristics. A two-year field study was planned to evaluate comparative effects of various Zn application approaches in bread wheat under plough tillage (PT) and zero tillage (ZT) system. Cultivation of wheat under ZT improved the soil organic carbon (17%), total soil porosity (11%), soil microbial biomass nitrogen (5%), and carbon (5%) in comparison to PT system averaged across the two years. Various efficiency indices were significantly influenced by Zn application methods during both years of experimentation. However, grain Zn contents were maximum with foliar-applied Zn in PT (31%) and soil-applied Zn under the ZT system (29.85%). Moreover, Zn use also enhanced the bioavailable Zn as lower phytate contents and phytate to Zn molar ratio were recorded. The highest bioavailable Zn was calculated for foliar (30%) and soil application (28%). Under both tillage systems, the maximum net benefits were obtained through Zn seed priming; nevertheless, ZT resulted in higher net benefits than PT due to low associated costs. In conclusion, Zn nutrition through different methods enhanced the productivity, profitability, and grain biofortification of wheat under PT and ZT systems.

Seed quality will remain the centerpiece of successful agricultural programs in the year 2000. As new changes occur in agriculture driven by advancements in biotechnology, seed enhancement technologies, a more diverse seed user clientele, and communication technologies, successful seed companies will require a knowledgeable and informed workforce to assure high seed quality. A new approach to seed technology training is professed that relies on the establishment of a three-institution consortium to achieve this objective. Advantages of the consortium are identified that emphasize the unique strengths of each institution, their geographic advantages representing major climactic/agricultural zones in the world, and differing approaches to seed technology training that are facilitated by increasing ease of global communication. This may be a better way to conduct seed technology training in the year 2000.

Manganese is an important essential micronutrient, and its deficiency causes latent health issues in humans. Agronomic biofortification can promisingly improve the plant nutrient concentration without changing the genetic makeup of plants. This study was designed to assess the best method of Mn application to enhance productivity and grain Mn contents under conventional tillage (CT) and no tillage (NT) systems. Manganese was delivered through seed coating (250-mg kg−1 seed), osmopriming (0.1-M Mn solution), soil application (1 kg ha−1), and foliar application (0.25-M Mn solution). A general control with no seed Mn application was included, whereas hydropriming and water spray were used as positive control treatments for Mn seed priming and Mn foliar spray, respectively. No tillage had a higher total soil porosity (9%), soil organic carbon (16%), soil microbial biomass carbon (4%), nitrogen (2%), and soil nutrients in the CT system. Manganese nutrition through various methods significantly enhanced the yield, grain biofortification, and net benefits for CT and NT systems. Averaged across two years, the maximum improvement in grain productivity was recorded with osmopriming (28%) followed by foliar application (26%). The highest grain Mn concentration (29% over no application) was recorded with Mn foliar applications under both tillage systems. Moreover, the highest economic returns and marginal net benefits were recorded with osmopriming. To improve the wheat production, profitability, and grain Mn concentration, Mn application through priming and foliar application may be opted. [ABSTRACT FROM AUTHOR]

Zinc (Zn) deficiency is a global concern for human health and causes a decrease in crop production and nutritional characteristics. A two-year field study was planned to evaluate comparative effects of various Zn application approaches in bread wheat under plough tillage (PT) and zero tillage (ZT) system. Cultivation of wheat under ZT improved the soil organic carbon (17%), total soil porosity (11%), soil microbial biomass nitrogen (5%), and carbon (5%) in comparison to PT system averaged across the two years. Various efficiency indices were significantly influenced by Zn application methods during both years of experimentation. However, grain Zn contents were maximum with foliar-applied Zn in PT (31%) and soil-applied Zn under the ZT system (29.85%). Moreover, Zn use also enhanced the bioavailable Zn as lower phytate contents and phytate to Zn molar ratio were recorded. The highest bioavailable Zn was calculated for foliar (30%) and soil application (28%). Under both tillage systems, the maximum net benefits were obtained through Zn seed priming; nevertheless, ZT resulted in higher net benefits than PT due to low associated costs. In conclusion, Zn nutrition through different methods enhanced the productivity, profitability, and grain biofortification of wheat under PT and ZT systems. [ABSTRACT FROM AUTHOR]