1. Different enzymatic antioxidative pathways operate within the sheep caruncular and intercaruncular endometrium throughout the estrous cycle and early pregnancy
- Author
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Kaïs H Al-Gubory, Catherine Garrel, Patrice Faure, Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay, Unité de Biochimie Hormonale - Nutritionnelle, Département de Biologie - Toxicologie - Pharmacologie, Centre Hospitalier Universitaire de Grenoble, Biologie du Développement et Reproduction (BDR), and École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)
- Subjects
0301 basic medicine ,Glutathione reductase ,sexual heat ,Endometrium ,chemistry.chemical_compound ,Food Animals ,ovin ,Pregnancy ,Conceptus ,endometrium ,Small Animals ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,caruncular and intercaruncular ,wether hoggs ,chemistry.chemical_classification ,early pregnancy ,biology ,Glutathione peroxidase ,estrous cycle ,cycle œstral ,Enzymes ,medicine.anatomical_structure ,endomètre ,Female ,progestérone ,medicine.medical_specialty ,sheep ,mouton ,Gene Expression Regulation, Enzymologic ,Superoxide dismutase ,Andrology ,03 medical and health sciences ,antioxydant enzymes ,Internal medicine ,medicine ,Animals ,Estrous cycle ,Equine ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Glutathione ,medicine.disease ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,chemistry ,enzyme antioxydante ,biology.protein ,Pregnancy, Animal ,Animal Science and Zoology ,plasma progesterone - Abstract
There has been a growing interest in the role played by antioxidant enzymes in the regulation of endometrial function in mammals. However, little is known about enzymatic antioxidative pathways involved in conditioning the cyclic and early pregnant endometrium for conceptus attachment and implantation in domestic ruminants. We aimed to investigate changes in activities of superoxide dismutase 1 and 2 (SOD1, SOD2), glutathione peroxidase (GPX), glutathione reductase (GR) and catalase (CAT) in sheep caruncles (CAR) and intercaruncles (ICAR) endometrial tissues of cyclic and early pregnant ewes. Irrespective of day of cycle or pregnancy, CAR demonstrated higher activities of SOD1 and SOD2 than in ICAR. On day 12 of the estrous cycle, ICAR demonstrated higher activity of GPX and GR than in CAR tissues. On days 12 and 16 the estrous cycle, ICAR demonstrated higher activity of CAT than in CAR. CAR demonstrated higher activity of GPX on day 18 than on days 4, 8, 12 and 16 of the estrous cycle. CAR demonstrated higher activity of CAT on day 18 than on days 4, 8, 12 and 16 of the estrous cycle. ICAR demonstrated higher activity of CAT on day 18 than on days 4, 8, and 16 of the estrous cycle. The activity of CAT in ICAR increased from days 4 and 8 to day 12 of the estrous cycle. The activity of SOD2 in CAR increased from day 16 to day 18 of pregnancy. On day 12 of pregnancy, CAR demonstrated higher activity of GPX than in ICAR. On day 16 of pregnancy, ICAR demonstrated higher activity of GPX than in CAR. The activity of GPX in ICAR increased from day 12 to day 16 of pregnancy. The activity of GPX in CAR increased from day 16 to day 18 of pregnancy. The activity of GR in CAR and ICAR increased from days 12 and 16 to day 18 of pregnancy. On days 16 and 18 of pregnancy, ICAR demonstrated higher activity of CAT than in CAR. The activity of CAT in CAR decreased from day 12 to days 16 and 18 of pregnancy. The activity of CAT in ICAR decreased from day 12 to day 16 of pregnancy and then increased from day 16 to day 18 of pregnancy. In conclusion, different antioxidant mechanisms operate within CAR and ICAR endometrium throughout the estrous cycle and during early pregnancy. This might be related to the different but important roles of CAR and ICAR endometrial tissues for the establishment of pregnancy.
- Published
- 2017
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