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The composition of the lipophylic extract from the sponge Suberites domuncula was investigated. Lipids and their fatty acids, as well as volatile compounds and sterols were identified. Stanols are the main class of steroids in the investigated sponge. A high concentration of unsaturated long chain fatty acids (C26–C28) was identified. The presence of branched and odd fatty acids indicates associated bacteria in the sponge.