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Arbuscular mycorrhizal fungi (AMF) might amend the toxicity of trace elements and improve the nutritional status of plants, but little is known about these responses in tropical native plants in iron-ore-impacted sites with excess zinc (Zn). We evaluated how the association with AMF contributes to the resistance capacity of Stryphnodendron adstringens to high Zn concentrations in mining waste. Seeds were sown in pots containing iron-ore mining waste with different concentrations of Zn (0, 100, 200, 400 and 600 mg L−1) and two conditions (inoculated or not with AMF). The biomass accumulation and physiological processes were severely impacted in the mining waste with Zn concentration above 100 mg L−1. The relative growth rate (RGR) and the concentration of phosphorus (P) in the shoot were higher in the AMF-inoculated plants, mainly when cultivated without Zn excess. Non-inoculated plants presented the lowest net photosynthetic rate (An) mainly due to impairment in light use efficiency, especially under Zn excess. The treatments did not change stomatal conductance, transpiration, Rubisco carboxylation rate, triose-phosphate utilization and respiration rate. The AMF improved the plant resistance capacity in mining waste substrate by promoting decreased oxidative damage, ameliorated P nutrition, higher RGR, root biomass and An. [ABSTRACT FROM AUTHOR]

This in vitro study aimed to evaluate the growth-inhibitory effects against periodontal disease-causing bacteria and cytotoxic effects against mouse fibroblast cells of the Stryphnodendron adstringens (barbatimão) hydroalcoholic extract. The contents of phenols and tannins in the extract were determined. The growth-inhibitory activity of the barbatimão was evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The viability of fibroblast cells was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide assay at 24 and 48 h post-treatment. The MIC values of the extract against Prevotella intermedia, Porphyromonas gingivalis , and Fusobacterium nucleatum were 0.05, 0.125, and 2 mg mL−1, respectively, while the MBC values were 4, 2, and 2 mg mL−1, respectively. The viability rate of barbatimão (0.25 mg mL−1)-treated L929 cells was higher than that of chlorhexidine (0.12%)-treated L929 cells at 48 h post-treatment. The contents of total phenolics and total tannins in the extract were 837.39 ± 0.10 and 785.82 ± 0.14 mg of tannic acid equivalent per gram of the extract, respectively. These findings indicate that the barbatimão hydroalcoholic extract, which exerted potent growth-inhibitory effects against the test microbial species and low cytotoxic effects on fibroblasts, has potential applications in the development of novel mouthwash products. [ABSTRACT FROM AUTHOR]

Keratoconus is an ectasia that leads to reduced stiffness of the cornea, which acquires a conical shape. Many studies are being carried out aiming at new treatments that lead to the recovery of these cross-links. Therefore, the efficiency of the Stryphnodendron adstringens butanolic extract in promoting cross-linking in corneal stroma of porcine eyes was analyzed. The influence of concentration of this plant extract to form cross-links was analyzed, as well as the influence of the contact time. The denaturation temperature results obtained for corneas treated with 1, 2, or 4% of the barbatimão butanolic extract show an increase of 3.84, 11.37, and 33.23%, respectively. These results suggest a rise in the number of cross-links. As for the contact time of the cornea with the 2% solution of the butanolic extract, it appears that the ideal time was 60 min. Enzymatic digestion assays confirm a decrease of 39% and 59% in the relative area and relative mass of corneas in the control group, while for the group treated with the barbatimão extract, these values were close to 0% and 9%, respectively. Therefore, it was possible to verify the efficiency of the barbatimão butanolic extract in modifying the stroma of porcine corneas, which promoted an increase in resistance to thermal and enzymatic degradation. [ABSTRACT FROM AUTHOR]

The present work aimed to evaluate the healing effect of hydrophilic polymeric resorbable biomembrane scaffolds containing plant extracts obtained from two different species, both popularly known as Stryphnodendron adstringens or Barbatimão. The hydrogel-based scaffolds were characterized and submitted to biological tests using Wistar rats to evaluate their healing capacity. The wound retraction index and the evaluation of the inflammatory process and tissue collagenization were recorded. The extracts showed antioxidant activity with IC50 between 10 and 20 µg/mL (DPPH assay) and 4–6 mmol Trolox/g (FRAP assay). The extract of Stryphnodendron adstringens (SA) presented gallocatechin, epigallocatechin, and O-methylpigalocatechin, while the extract of Abarema cochliacarpa (AC) presented catechin, dimers of procyanidins, di-O-hydroxide, O-deoxyhexosi-hexoside, and epicatechin. The membranes containing SA extract (GELSA) were more rigid, with a more intense color, but less thick, with a more compact structure and few pores. The membranes containing AC extract (GELAC) presented a mechanical profile like the gelatin membrane (GEL), with greater permeability to water vapor. The GELAC and GELSA membranes showed similar thermal degradation profiles. The wounds treated with the membranes containing the extracts obtained high levels of retraction of the wounds with values around 60% and 80% in three and seven days, respectively. These data indicate that the compounds of both species have promising biological activities in the repair process, showing that the extracts accelerated the healing process due to the lower intensity of the inflammatory reaction and the presence of compounds such as catechin and epigallocatechin. [ABSTRACT FROM AUTHOR]

Stryphnodendron adstringens is a medicinal plant that has a broad spectrum of action, including antibacterial activity. The aim of the present study was to evaluate the effect of S. adstringens alone and in combination with potassium sorbate (PS) against foodborne bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and, for most of the bacteria tested, the crude extract (CE), aqueous fraction (AQF), and ethyl-acetate fraction (EAF) of S. adstringens had a MIC and MBC ranging from 500 to ≥ 1000 µg/mL. The AQF and EAF showed greater activity against S. aureus strains (MIC = 125 to 250 µg/mL; MBC = 500 to 1000 µg/m). Quantitative cell viability was determined and was observed reductions ranging from 3.0 to 5.8 log10 CFU/ml.The combination of S. adstringens and PS against seven S. aureus isolates was determined by the checkerboard method at neutral and acid pH. In a neutral medium, the AQF + PS combination presented synergistic or additive interactions against six S. aureus strains. The combination of EAF + PS resulted in additive interactions against four bacterial isolates. In an acidic medium, the AQF + PS combination was synergistic or additive against all S. aureus, while EAF + PS presented the same effect against six S. aureus strains S. adstringens showed important antibacterial effects against foodborne S. aureus strains. Moreover, the combination of S. adstringens fractions and PS improved the antibacterial activity compared to the compounds utilized individually. The combined use of these compounds may be an alternative to reduce bacterial food contamination and improve food safety. [ABSTRACT FROM AUTHOR]

Abstract The plant Stryphnodendron adstringens is a species of legume in the genus Stryphnodendron that is found in Brazil. It is also known as “barbatimão-verdadeiro” and it is popularly used in folk medicine due to its healing properties. Purpose: The aim of this study was to evaluate the healing activity of hydrogel, together with an S. adstringens hydroalcoholic extract, in diabetic and non-diabetic rats. Methods: The phytochemical analysis of the S. adstringens hydroalcoholic extract was evaluated through HPLC and its antioxidant activity was measured by the DPPH assay. The cytotoxicity, the scratch assay, and the fibroblast cell proliferation were also evaluated. Forty (40) Wistar rats were submitted to an excision for a full-thickness wound of 1.5 cm × 1.5 cm in their dorsum. The animals were treated twice a day over 16 days, with a rich layer of the corresponding treatment: Hydrogel; Hydrogel associated with 5% S. adstringens hydroalcoholic extract (H + SAHE); S. adstringens hydroalcoholic extract (SAHE); Collagenase with Chloramphenicol; or Carbopol. Morphological and histological analyses of the injury were performed. Results: The phytochemical analysis of SAHE indicated the presence of phenolic compounds, tannins, and flavonoids. The hydroalcoholic extract showed strong antioxidant activities (IC50 = 25.56 ± 1.04 μg/mL). H + SAHE induced the fibroblast proliferation (148 ± 6.9%) and it was not cytotoxic. The association with H + SAHE showed a more pronounced healing activity than did the other treatments in the non-diabetic animals and in the diabetic animals, hence, promoting angiogenesis and reepithelialization. Conclusion: Under these scenarios, this study has demonstrated effectiveness in the recovering wounds of diabetic rats. Graphical abstract

ABSTRACT Background: There is still a need for progress in the treatment of transsphincteric anal fistulae and the use of herbal medicines seems promising. Aim: To evaluate the efficacy of Stryphnodendron adstringens associated with fistulotomy and primary sphincteroplasty in the treatment of transsphincteric fistulae in rats. Methods: Thirty Wistar rats were used, which were submitted to transsphincteric fistulas with steel wire 0; after 30 days a treatment was performed according to the group. Group A (n=10) was submitted to fistulotomy; group B (n=10), fistulotomy followed by primary sphincteroplasty with “U” stitch with polyglactin 911 4-0; group C(n=10) , similar to group B, but with the interposition between the muscular stumps of hemostatic sponge soaked in Stryphnodendron adstringens extract. Euthanasia was performed after 14 days, resecting a segment of the anal canal for histological analysis, which aimed to evaluate the closure of the fistula, the area of separation of the muscle cables, the inflammatory process and the degree of fibrosis. Results: No animal had a remaining fistulous tract. About the spacing between the muscle cables, an average of 106.3 µm2 was observed in group A, 82.8 µm2 in group B and 51.8 µm2 in group C (p

ABSTRACT: Fasciolosis has been diagnosed in cattle, goats, sheep and horses in southern and southeastern Brazil. Effective alternative treatments are the targets of study. One promising alternative is the use of plant extracts. The aim of this study was to perform phytochemical analysis of extracts of Eugenia uniflora L., Harpagophytum procumbens, Psidium guajava L. and Stryphnodendron adstringens, and to evaluate the in vitro efficacy of these extracts on ovicidal activity in Fasciola hepatica. Plant extracts were analyzed for phytochemical properties. F. hepatica eggs were collected directly from the gallbladders of animals diagnosed as positive for fasciolosis on post mortem examination. One hundred eggs were incubated with 3 ml of each extract at concentrations of 0.10%, 0.25% and 0.50%, albendazole 0.50% (positive control) or tap water (negative control). To determine anti larval efficacy of each plant extract, hatched eggs were counted and the averages were used. Phytochemical analysis revealed the presence of phenolic compounds, tannins and terpenes in most extracts. E. uniflora L. extract was 100% effective at 0.10%, H. procumbens was effective at 0.25% and P. guajava L. and S. adstringens extracts were 100% effective at all concentrations tested. Taken together, the data suggested that ovicidal activity in F. hepatica is due to the presence of these bioactive compounds.

The plant Stryphnodendron adstringens is a species of legume in the genus Stryphnodendron that is found in Brazil. It is also known as "barbatimão-verdadeiro" and it is popularly used in folk medicine due to its healing properties. Purpose: The aim of this study was to evaluate the healing activity of hydrogel, together with an S. adstringens hydroalcoholic extract, in diabetic and non-diabetic rats. Methods: The phytochemical analysis of the S. adstringens hydroalcoholic extract was evaluated through HPLC and its antioxidant activity was measured by the DPPH assay. The cytotoxicity, the scratch assay, and the fibroblast cell proliferation were also evaluated. Forty (40) Wistar rats were submitted to an excision for a full-thickness wound of 1.5 cm × 1.5 cm in their dorsum. The animals were treated twice a day over 16 days, with a rich layer of the corresponding treatment: Hydrogel; Hydrogel associated with 5% S. adstringens hydroalcoholic extract (H + SAHE); S. adstringens hydroalcoholic extract (SAHE); Collagenase with Chloramphenicol; or Carbopol. Morphological and histological analyses of the injury were performed. Results: The phytochemical analysis of SAHE indicated the presence of phenolic compounds, tannins, and flavonoids. The hydroalcoholic extract showed strong antioxidant activities (IC50 = 25.56 ± 1.04 μg/mL). H + SAHE induced the fibroblast proliferation (148 ± 6.9%) and it was not cytotoxic. The association with H + SAHE showed a more pronounced healing activity than did the other treatments in the non-diabetic animals and in the diabetic animals, hence, promoting angiogenesis and reepithelialization. Conclusion: Under these scenarios, this study has demonstrated effectiveness in the recovering wounds of diabetic rats. [ABSTRACT FROM AUTHOR]

Stryphnodendron adstringens is a typical tree from Brazilian Savanah used in medicine as an anti-inflammatory and antiseptic agent. It is secondary metabolites has biological activities, so the development of efficient extraction methods is essential. Microwave irradiation through assisted extraction is innovative and highly efficient for bioactive compounds. The aim of this study was to optimize an extractive method for phenolics compounds, as tannins, from the stem bark of "barbatimão" by microwave irradiation using a statistical planning and to evaluate its consistency with conventional extraction. Microwave irradiation extraction, 16.36–22.12% of phenols and 15.91–18.69% of tannins, has a better yield when compared to conventional extraction, 14.99% of phenols and 16.70% of tannins. The method by microwave irradiation is consistent with the conventional one. However, extraction by microwave irradiation had a reduction in reaction time, reagent volume, samples amount and energy consumption when compared to conventional extraction. [ABSTRACT FROM AUTHOR]

We evaluated the activity of the aqueous fraction and the ethyl acetate fraction of Stryphnodendron adstringens against Staphylococcus aureus and proposed their mechanism of action. The antibacterial activity of S. adstringens fractions was evaluated against S. aureus and the cell targets were rated by docking. The fractions showed moderate antibacterial activity against S. aureus without toxicity on two mammalian cell lines. They also showed synergistic antibacterial activity with tannic acid (TA). In silico assays indicated FabG, FabZ and FabI as probable targets. The metabolic pathway for fatty acid biosynthesis in S. aureus was affected by components of S. adstringens. The synergistic effect when combining TA with S. adstringens fractions suggests a natural alternative to S. aureus control. Significance and Impact of the Study: This is the first study describing the possible targets of action of Stryphnodendron adstringens on Staphylococcus aureus. Molecular dynamics simulations showed that the components of S. adstringens affected the metabolic pathway for fatty acid biosynthesis (FAS II) in S. aureus, inhibiting the FabI, FabG and FabZ enzymes. As tannic acid (TA) is a known inhibitor of some targets identified, we showed synergistic antibacterial activity of S. adstringens in combination with TA. This combination did not show toxicity against HaCaT and Vero cells and based on all these results we suggest that S. adstringens can be a natural and sustainable alternative to S. aureus control. [ABSTRACT FROM AUTHOR]

Keratoconus is a progressive disease that affects patients' quality of life by producing corneal irregular astigmatism and low vision as the cornea becomes thinner and presents a conical protrusion. Inducing cross-linking in keratoconic corneal stroma is the current preventive intervention that effectively blocks the progression of this ectasia. As a result of this process, there is an increase in biomechanical strength and resistance of the cornea. In this context, two plant extracts rich in polyphenols (Euterpe oleracea and Stryphnodendron adstringens) have been tested in this study to compact and stiffen the tissue, and its effect was evaluated by enzymatic digestion of porcine corneas. Thermal analysis by DSC was employed to verify the corneal resistance to degradation induced by the plant extracts. The values of temperature and enthalpy of denaturation at the initial time were: 65.6 ± 0.3 °C/3.8 ± 0.3 J g−1; 79.4 ± 0.5 °C/5.5 ± 0.4 J g−1; and 93.1 ± 1.2 °C/8.0 ± 0.6 J g−1, respectively, for the control, E. oleracea, and S. adstringens groups. After 24 h of collagenase digestion, these values were: 60.1 ± 0.8 °C/1.3 ± 0.1 J g−1; 78.1 ± 1.0 °C/5.6 ± 0.6 J g−1; and 93.2 ± 0.6 °C/8.1 ± 0.06 J g−1, respectively. These results show that there was no decrease in temperature and enthalpy of denaturation (p < 0.01) in corneas of the groups treated with the E. oleracea or S. adstringens extracts. However, in the control group, the temperature and the enthalpy decreased by approximately 8% and 65%, respectively. Therefore, this work demonstrated for the first time that plant extracts rich in polyphenols were efficient in decreasing the digestion and increasing the denaturation temperature of corneas. [ABSTRACT FROM AUTHOR]

The scarcity of nonrenewable resources and the increase in environmental pollution have intensified the search for materials that exhibit specific characteristics and are nontoxic, renewable, and sustainable. Thus, the objective of this work was to produce natural polyphenol adhesives reinforced with rice husk and its ash to increase the mechanical resistance and moisture resistance of the glue line in wood bonded joints. Polyphenols were extracted from the bark of Stryphnodendron adstringens (Mart.) Coville (barbatimão). Adhesives were produced with a 50 % solid and 50 % liquid composition. Rice husk and husk ash underwent X-ray fluorescence analysis (XRF). Adhesives and reinforcement material were characterized by Fourier transform infrared (FTIR) and thermogravimetric analyses (TGA). The adhesives were glued in a mechanical press in specimens made of Pinus elliottii , which were subjected to shear testing of the wet and dry glue line. As a result, the chemical components present in rice husk and its ash positively influenced the properties of the adhesives. The mechanical glue line shear test showed that the adhesive reinforced with rice husk ash did not show a statistically significant difference. However, natural adhesives based on polyphenols from barbatimão strengthened with rice husk and ash showed improved properties, demonstrating how much it pays to use the residue of rice production to reinforce the matrix of tannin adhesives. Thus, it can be determined that reinforcement with rice husk and ash is efficient in improving some properties of natural adhesives based on polyphenols. [Display omitted] • Sustainable production by applying the residue of rice crop production • Use of a forest specie from cerrado for tannin extraction and adhesive production • The reinforcement with residue from rice production in natural adhesives improved their characteristics [ABSTRACT FROM AUTHOR]

Medicinal plants have been recognized as hosts of high diverse endophytic microorganisms, including fungi that produce secondary metabolites with biological activity. Two biomes in Brazil, Pantanal (wetland), and Cerrado (savannah), are known as biodiversity hotspots, and despite their importance as a reservoir for several species, knowledge about the fungal biodiversity in these biomes is very limited. Fungal endophytic communities associated with leaves and petioles of the medicinal plants Vochysia divergens (from Pantanal) and Stryphnodendron adstringens (from Cerrado) were analyzed and studied for their antimicrobial activity against human and plant pathogens. A total of 1,146 isolates of endophytic fungi were obtained from plants collected in January and June of 2016 and grouped into 124 morphotypes. One isolate of each morphotype was identified by sequencing of internal transcribed spacer (ITS) region of the rDNA gene, which revealed the presence of 24 genera, including 3 possible new genera, and 48 taxa. Differences in the endophytic community according to the biomes were observed concerning the analyzed morphotypes. However, when we analyzed the diversity of genera and richness, they were similar for both plants, with Diaporthe, Phyllosticta, and Neofusicoccum as dominant genera. In addition, the community composition of V. divergens differs according to the analyzed plant tissues (petiole and leaf). These data suggested that both, the plant species and plant tissues play a role in the composition of endophytic community. As regards the biotechnological potential, 5 isolates showed activity against the phytopathogens Phyllosticta citricarpa, Colletotrichum abscissum, and Fusarium verticilioides, and 8 isolates showed high activity against clinical pathogens and were selected for the production of crude extract in different culture media. Extract from cultivation of Diaporthe sp. LGMF1548 and LGMF1583 and Neofusicoccum brasiliense LGMF1535 showed activity against methicillin-resistant Staphylococcus aureus, Klebssiella pneumonia, and Candida albicans. In addition, extracts of Diaporthe cf. heveae LGMF1631 inhibited 90% of the mycelial growth of the P. citricarpa and 70% of C. abscissum and may represent an alternative to be used in the biological control of these phytopathogens. Future research will focus on the chemical characterization and structural elucidation of these bioactive compounds.

Medicinal plants have been recognized as hosts of high diverse endophytic microorganisms, including fungi that produce secondary metabolites with biological activity. Two biomes in Brazil, Pantanal (wetland), and Cerrado (savannah), are known as biodiversity hotspots, and despite their importance as a reservoir for several species, knowledge about the fungal biodiversity in these biomes is very limited. Fungal endophytic communities associated with leaves and petioles of the medicinal plants Vochysia divergens (from Pantanal) and Stryphnodendron adstringens (from Cerrado) were analyzed and studied for their antimicrobial activity against human and plant pathogens. A total of 1,146 isolates of endophytic fungi were obtained from plants collected in January and June of 2016 and grouped into 124 morphotypes. One isolate of each morphotype was identified by sequencing of internal transcribed spacer (ITS) region of the rDNA gene, which revealed the presence of 24 genera, including 3 possible new genera, and 48 taxa. Differences in the endophytic community according to the biomes were observed concerning the analyzed morphotypes. However, when we analyzed the diversity of genera and richness, they were similar for both plants, with Diaporthe, Phyllosticta, and Neofusicoccum as dominant genera. In addition, the community composition of V. divergens differs according to the analyzed plant tissues (petiole and leaf). These data suggested that both, the plant species and plant tissues play a role in the composition of endophytic community. As regards the biotechnological potential, 5 isolates showed activity against the phytopathogens Phyllosticta citricarpa, Colletotrichum abscissum, and Fusarium verticilioides, and 8 isolates showed high activity against clinical pathogens and were selected for the production of crude extract in different culture media. Extract from cultivation of Diaporthe sp. LGMF1548 and LGMF1583 and Neofusicoccum brasiliense LGMF1535 showed activity against methicillin-resistant Staphylococcus aureus, Klebssiella pneumonia, and Candida albicans. In addition, extracts of Diaporthe cf. heveae LGMF1631 inhibited 90% of the mycelial growth of the P. citricarpa and 70% of C. abscissum and may represent an alternative to be used in the biological control of these phytopathogens. Future research will focus on the chemical characterization and structural elucidation of these bioactive compounds. [ABSTRACT FROM AUTHOR]

Keratoconus is an eye ectasia that affects the cornea causing distortion of vision. Corneal crosslinking promoted by riboflavin photo-stimulated at 365 nm is a treatment used successfully to block the keratoconus progression. In this study, we characterize rabbit corneas subjected to enhanced crosslinking using different vegetable extracts prepared from Stryphnodendron adstringens (Mart.) Coville bark by differential scanning calorimetry. The extracts tested in this study were: aqueous, ketonic, ethanolic, ethyl acetate and hydroalcoholic, which revealed high levels of polyphenols, such as proanthocyanidins. The corneas, obtained from the slaughter rabbits, were divided into groups: control (consisting of untreated corneal samples) and S. adstringens, where the samples were subjected to 4% vegetable extracts for 2 h. The DSC profiles of the corneas were obtained in nitrogen atmosphere. The results showed that temperature and enthalpy of denaturation of the corneal samples treated with S. adstringens were higher than the control group. The control group presents temperature and enthalpy values of 59.8 ± 1.1 °C and 24.6 ± 2.7 J g, respectively. After crosslinking with ketone extract, the values were 90.1 ± 1.1 °C and 39.9 ± 2.7 J g. These results show that S. adstringens is efficient to increment the crosslinking level of the corneas. [ABSTRACT FROM AUTHOR]

Copyright of Pesquisa Veterinaria Brasileira is the property of Colegio Brasileiro de Patologia Animal - CBPA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Arquivo Brasileiro de Medicina Veterinária e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Pesquisa Veterinaria Brasileira is the property of Colegio Brasileiro de Patologia Animal - CBPA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Stryphnodendron adstringens (Mart.) Coville (barbatimão) is a tree belonging to the Fabaceae family, and it is commonly found in the southeastern Brazilian cerrado. The stem bark of this tree is traditionally used as an anti-inflammatory and antiseptic agent to treat leucorrhea and diarrhea, as well as to promote wound healing (owing to the presence of proanthocyanidins). Proanthocyanidins were obtained from the ethanolic extract of S. adstringens stem bark and assessed by reversed phase-high performance liquid chromatography with an ultraviolet/diode array detector. The identified compounds included gallic acid, catechin, gallocatechin (GC), epigallocatechin, and epigallocatechin gallate (EGCG). The selected markers, GC and EGCG, were simultaneously used for chromatographic validation (linearity range: 30-330 ng, equivalent to 3-33 µg/mL; r>0.998). The method showed precision (intra-day relative standard deviation [RSD]: 1.72% for GC; 1.16% for EGCG; inter-day RSD: 1.74%-2.60% for both markers), accuracy, robustness, and selectivity. The limits of detection and quantitation were 0.29 µg/ml and 0.89 µg/ml for GC, and 0.88 µg/mL and 2.67 µg/mL for EGCG, respectively. In addition, S. obovatum was evaluated and showed an average of 12.2 µg/mL for GC (equivalent to 1.22% w/w) and 14.2 µg/mL for EGCG (equivalent to 1.42% w/w) in the ethanolic extract. The quantitative results were compared to those obtained for S. adstringens, which showed that the markers are present in both species.

The aim of this study was to evaluate the insecticidal effect of aqueous, alcoholic, and oil extracts from leaves of eight medicinal plants against Diabrotica speciosa prepared at five concentrations. The extracts that used commercial soybean oil as solvent showed the highest D. speciosa mortality due to the solvent itself, regardless of the used plants and their concentrations. Thus, commercial soybean oil was discarded as solvent since at these volumes it would cause serious phytotoxicity problems. After 24 hours of exposure of the pest to the extracts, the highest D. speciosa mortality values were observed for Copaifera langsdorfii and Chenopodium ambrosioides extracts, both in 5% alcohol, and Artemisia verlotorum, in 10% water. However, in the last mortality assessment (48 h), C. langsdorfii extract in 5% alcohol showed higher mortality of this pest, followed by C. ambrosioides extract in 5% alcohol, compared to the remaining plants.O objetivo deste trabalho foi avaliar o efeito inseticida de extratos aquosos, alcoólicos e oleosos de folhas de oito plantas medicinais contra Diabrotica speciosa preparadas em cinco concentrações. Os extratos que utilizaram óleo de soja comercial como solvente apresentaram as maiores mortalidades de D. speciosa em função do próprio óleo, independentemente das plantas utilizadas em suas concentrações. Sendo assim, o óleo de soja comercial foi descartado como solvente, pois nestes volumes acarretaria sérios problemas de fitotoxidade. Após 24 horas de exposição da praga aos extratos, os maiores valores de mortalidade de D. speciosa foram observados nos extratos de Copaifera Langsdorfii e de Chenopodium ambrosioides, ambos em álcool 5%, e de Artemisia verlotorum, em água 10%. Entretanto, na última avaliação de mortalidade (48 h), o extrato de C. langsdorfii em álcool a 5% apresentou maior mortalidade dessa praga, seguida pelo extrato alcoólico a 5% de C. ambrosioides comparada às demais plantas.

Sabe-se que o número de casos de câncer de pele tem crescido muito nos últimos anos. Com isso, novos produtos são constantemente desenvolvidos no intuito de aumentar a gama de substâncias para proteção solar. O principal objetivo desse trabalho foi a análise in vitro da propriedade fotoprotetora de uma formulação contendo filtros solares químicos, adicionada de extratos brutos de Guazuma ulmifolia, Maytenus ilicifolia, Stryphnodendron adstringens e Trichilia catigua, respectivamente, e a avaliação quanto ao acréscimo do Fator de Proteção Solar (FPS). O ensaio foi conduzido pelo método espectrofotométrico in vitro do Fator de Proteção Solar (FPS). O teor de Polifenóis Totais (PT) e sua correlação com a Capacidade Antioxidante (CA) dos extratos avaliados foram realizados. Os resultados demonstram que as formulações foram estáveis após a adição dos extratos, quanto a características físicoquímicas (características macroscópicas, ensaio de centrifugação e pH) quando comparadas ao controle. O ensaio de FPS in vitro demonstrou decaimento do FPS das formulações acrescidas de extratos vegetais ricas em compostos fenólicos, exceto para o extrato de S. adstringens. Os FPS obtidos para os extratos foram ≤2. O teor em PT foram (%): G. ulmifolia, 24,26±0,34; M. ilicifolia, 14,66±0,18; S. adstringens, 34,38±0,62 e T. catigua, 41,60±0,13. Os valores da CA (IC50) frente ao radical DPPH foram (μg/mL): vitamina C (7,52±0,12), G. ulmifolia (8,94±0,13), M. ilicifolia (38,41±0,93), S. adstringens (7,31±0,15) e T. catigua (5,48±0,04). A CA foi diretamente proporcional ao teor de PT.

A candidíase vaginal é uma doença causada, na maioria das vezes, pelo fungo do gênero Candida sp, que habita o trato gastrintestinal e geniturinário da espécie humana e pode tornar-se patogênico sob determinadas condições. A maioria dos indivíduos desenvolve defesas imunológicas que impedem a proliferação e desenvolvimento de candidíase localizada ou disseminada. Embora a causa exata do aumento de espécies não-albicans seja desconhecida, há evidências de que a própria terapia antifúngica possa estar contribuindo para o processo. Linhagens de C. glabrata são mais resistentes aos imidazólicos do que a C. albicans, sendo necessária uma concentração 10 vezes superior de miconazol para eliminar a C. glabrata quando comparada a C. albicans. Foi realizado um antifungigrama testando o potencial de ação de produtos vegetais sobre o fungo Candida sp. Foi observado que, ocorreu inibição do fungo no contato com os extratos hidroglicólicos das plantas Arctium lappa L., Calendula officinalis L., Stryphnodendron adstringens (Mart.) Coville e Tabebuia avellanedae Lorentz ex Griseb. A importância deste trabalho reside na possibilidade de desenvolvimento de tratamento complementar, menos agressivo, de menor custo e sem toxidade, o que possibilitaria melhor qualidade de vida para portadoras de candidíase vaginal recorrente ou não.Vaginal candidiasis is a disease caused, in most cases, by the fungus of the genus Candida sp., which inhabits the gastrointestinal and genitourinary tracts of the human species and can become pathogenic under certain conditions. Most individuals develop immune defenses that prevent the proliferation and the development of localized or disseminated candidiasis. Although the exact cause of the increase in non-albicans species is unknown, there is evidence that antifungal therapy itself may have contributed to it. Strains of C. glabrata are more resistant to imidazole than C. albicans, and a 10-fold higher concentration of miconazole is required to eliminate C. glabrata compared to C. albicans. Etest was performed by testing the action potential of plant products on the fungus Candida sp. Inhibition of the fungus occurred when it was in contact with the hydroglycol extracts of plants Arctium lappa L., Calendula officinalis L., Stryphnodendron adstringens (Mart.) Coville and Tabebuia avellanedae Lorentz ex Griseb. The importance of this study lies in the possibility of developing a complementary treatment, less aggressive, of lower cost and without toxicity, which would allow better life quality for women with vaginal candidiases that are recurrent or not.

The aim of this study was to evaluate the in vitro antimicrobial activity of Lippia sidoides essential oil (LsEO), popularly known as “rosemary-pepper”, and the Stryphnodendron adstringens dry crude extract (SaDCE), or “barbatimao”, against bacteria isolated from total milk flock, from small farms of northern Minas Gerais state. SaDCE was obtained from the peel of the vegetable through static distillation in ethanol 99.9% during eight days. LsEO was obtained through hydro-distillation of its fresh leaves. The bacteria Escherichia coli and Staphylococcus aureus isolated from milk underwent the test of disk-diffusion and minimum bacterial concentration (MBC), using concentrations of 320, 160, 80, 40, 20 and 0μl/mL of LsEO and 400, 200, 100, 50, 25 and 0mg/mL of SaDCE. All bacteria were sensitive to the vegetable extracts, except the Escherichia coli which was not inhibited by any test when SaDCE was used.

A cria ensacada brasileira é uma das piores enfermidades de abelhas Apis mellifera L. africanizadas, principalmente do Sudeste do Brasil. Ela afeta o desenvolvimento das larvas, após serem alimentadas pelas abelhas nutrizes com o pólen do barbatimão (Stryphnodendron polyphyllum e Stryphnodendron adstringens), ocasionando a morte entre os estágios de pré-pupa e pupa. O objetivo deste trabalho foi verificar o efeito do substituto de pólen na redução da mortalidade da cria ensacada brasileira durante o florescimento do Stryphnodendron adstringens. Foram utilizados dois tratamentos: as colmeias do tratamento 1 receberam semanalmente substituto de pólen (mistura de farinha de soja, fubá e farinha de trigo em proporções iguais). As colmeias do tratamento 2 não receberam o substituto de pólen, tendo como único alimento o pólen que encontravam na natureza. As colmeias alimentadas apresentaram diferença significativa de 93,5% de viabilidade de sobrevivência de cria em relação às não alimentadas, que apresentaram 80,4% de sobrevivência. A frequência de pólen S. adstringens nos coletores de pólen, no tratamento 1, foi 0,21. Entretanto, no Tratamento 2, foi de 4,38, indicando que as colmeias que não se alimentaram com o substituto apresentaram maior frequência do pólen dessa planta, quando comparadas com as que dispunham da dieta artificial. Esse manejo sugere que a oferta do substituto de pólen, antes e durante a florada do Stryphnodendron adstringens, é importante na redução dos efeitos da cria ensacada brasileira em enxames de abelhas africanizadas.Brazilian sac brood is one of the worst diseases of Apis mellifera L. mainly in southeast Brazil. This disease affects the larval development after having been fed with "barbatimão" pollen (Stryphnodendron polyphyllum and Stryphnodendron adstringens); the workers become intoxicated and die between the prepupae and pupae stages. This study verified the effect of pollen substitute on the reduction of Apis mellifera L. mortality, caused by Brazilian sac brood during Stryphnodendron adstringens flowering. Two treatments were used: in treatment one, colonies received pollen substitute weekly (a blend of a soy flour, maize flour and wheat flour of equal proportions). The hives of treatment 2 didn´t receive the replacement of pollen substitute, receiving only the pollen found in nature. The hives which were fed with the pollen substitute had significant difference of 93.5% of brood survival viability when compared to non-fed which had 80.4% of survival. The frequency of pollen S. adstringens in pollen traps, in treatment 1, was of 0.21. However, in treatment 2, the frequency was of 4.38, indicating that the hives that were not fed with the substitute showed a higher frequency of pollen of this plant when compared with those who had the artificial diet. The results suggest that supplying with pollen substitute, before and during Stryphnodendron adstringens flowering, is important in reducing the effects of Brazilian sac brood disease.

Teve como objetivo avaliar a atividade antimicrobiana in vitro do óleo essencial do Lippia sidoides (OELs) conhecido popularmente como “alecrim-pimenta” e do extrato bruto seco do Stryphnodendron adstringens (EBSSa) “barbatimão” em bactérias isoladas do leite total de rebanho, provenientes de unidades agrícolas familiares do norte de Minas Gerais. O EBSSa foi obtido a partir da casca por extração estática em etanol 99.9% por oito dias. O OELs foi obtido por meio da hidro-destilação das folhas frescas. As bactérias foram isoladas do leite total do rebanho de amostras de leite de propriedades rurais do Norte de Minas Gerais e posteriormente foram identificadas por métodos padrões. Foram submetidas aos testes de disco-difusão e concentração bactericida mínima (CBM), utilizando as concentrações de 320, 160, 80, 40, 20 e 0µl/mL do OELs e 400, 200, 100, 50, 25 e 0mg/mL do EBSSa. Todas as bactérias se mostraram sensíveis aos extratos vegetais, exceto a Escherichia coli para os dois testes quando foi utilizado o EBSSa.

Stryphnodendron adstringens (Mart.) Coville, 1910 is a small tree, distributed widely throughout the Cerrado region of Brazil and named "barbatimão" by the Tupi-Guarani tribes, which presents astringent properties. Its ethnopharmacological uses comprise, among others, anti-inflammatory and wound healing action, and it is used in the treatment of diarrhea and gynecological problems. The phytotherapeutic use of 'barbatimão' is largely related to its tannin content, which is abundant in its bark. The main goal of the present study was to evaluate the cytotoxic, mutagenic, and genotoxic potential of the lyophilized solution of the stem bark of S. adstringens, using the Ames test, the SOS-Inductest and the SOS-Chromotest. S. adstringens presented cytotoxic activity in all tested systems, did not present mutagenic activity detectable by the Ames test and SOS-Chromotest, and showed some genotoxic effect on the SOS-Inductest. However, the metabolization of the extract by S9 fraction attenuated its genotoxic and cytotoxic activities.Stryphnodendron adstringens (Mart.) Coville, 1910 é uma pequena árvore amplamente distribuída nas regiões de cerrado do Brasil, chamada de "barbatimão" pelas tribos Tupi-Guarani, que apresenta propriedade adstringente. Seu uso etnofarmacológico compreende, entre outros, efeitos antiinflamatório e cicatrizante, sendo empregada no tratamento de diarréias e problemas ginecológicos. Grande parte das aplicações do fitoterápico de barbatimão está relacionada aos taninos, abundantes em sua casca. O objetivo do presente trabalho foi avaliar os potenciais citotóxico, mutagênico e genotóxico da solução liofilizada da casca de S. adstringens, utilizando Teste de Ames, SOS-Induteste e SOS-Cromoteste. S. adstringens apresentou atividade citotóxica em todos os sistemas testados, não apresentou atividade mutagênica detectável pelo teste de Ames e SOS-Cromoteste e mostrou certo efeito genotóxico no SOS-Induteste. Porém, a metabolização do extrato pela fração S9 atenuou suas atividades genotóxica e citotóxica.

This study evaluated the cicatrizant effect of an ointment containing 1% of the ethyl-acetate fraction extracted from the stem bark of "barbatimão" (Stryphnodendron adstringens), in wounds made in the skin of rats, after 4, 7 and 10 days of treatment. Control wounds were treated with a base ointment without extract. The proliferation of keratinocytes in the area of reepithelialization was evaluated by counting the number of epithelial cells that were blocked in metaphase by vincristine sulfate. The length of the reepithelialized margin and the contraction of the wound were measured. Topical application of the "barbatimão" ointment stimulated proliferation of the keratinocytes, but had no effect on the length of the epithelium or on the contraction of the wounds.Neste estudo, avaliou-se a atividade cicatrizante de uma pomada contendo uma fração acetato de etila 1% obtida de cascas de "barbatimão" (Stryphnodendron adstringens) em feridas excisionais na pele de ratos após 4, 7 e 10 dias de tratamento. Feridas controle foram tratadas com pomada base, sem extrato. A proliferação dos queratinócitos na área reepitelizada foi avaliada através da contagem do número de queratinócitos bloqueados em metáfase, pelo sulfato de vincristina. O comprimento da margem reepitelizada e a contração das feridas foram mensurados. As feridas tratadas com barbatimão apresentaram um maior número de mitoses do que aquelas tratadas com a pomada base, em todos os tempos avaliados. A aplicação tópica da pomada de "barbatimão" estimulou a proliferação epitelial contudo não teve efeito sobre a migração dos queratinócitos ou sobre a contração das feridas.

O estudo objetivou realizar ensaio toxicológico pré-clínico inicial para investigar a toxicidade das folhas de alecrim-pimenta (Lippia sidoides Cham.), aroeira (Myracrodruon urundeuva Fr. All.) e barbatimão [Stryphnodendron adstringens (Mart.) Coville] e do farelo da casca de pequi (Caryocar brasiliense Camb.), por meio da determinação da dose letal 50% (DL50). Na investigação da DL50, foram utilizados grupos de camundongos Swiss de mesmo sexo (n=150, sendo 30 animais por tratamento) inoculados, por via intraperitoneal, com diluições seriadas do extrato hidroalcoólico das folhas de alecrim, aroeira e barbatimão e do farelo da casca de pequi. Após a inoculação, os animais foram observados por um período de 14 dias, para determinar a quantidade de mortos, doentes e sobreviventes. O estudo toxicológico pré-clínico agudo demonstrou, em camundongos por via intraperitoneal, toxicidade igual a 0,31mg mL-1 DL50 mL-1 para todas as plantas, exceto para o barbatimão, que apresentou toxicidade igual a 0,25mg mL-1. Pesquisas devem ser realizadas visando a obter dados de toxicidade das plantas em outras vias para assegurar o uso em saúde humana e animal.This study aimed to conductpre-clinical toxicology testing to investigate the toxicity of Lippia sidoides Cham., Myracrodruon urundeuva Fr. All., Stryphnodendron adstringens (Mart.) Coville and Caryocar brasiliense Camb., by determining the 50% (LDL50) lethal dose. In the investigation of the LD50, groups of Swiss mice of the same sex were used (n=150; 30 animals per plant and all inoculated intraperitoneally with serial dilutions of the aqueous fraction obtained from the leaves of plants. After inoculation, the animals were observed along a period of 14 days in order to observe the dead, sick and survivors rate. In mice inoculated intraperitoneally, the acute pre-clinical toxicology testing demonstrated toxicity equal to 0,31mg mL-1 LD50 mL-1 for all plants. The exception was for Stryphnodendron adstringens, which presented toxicity equal to a 0,25mg mL-1DL50 mL-1 and values expressed in terms of dilution. Research should be conducted to obtain data on toxicity of the plants in other ways to ensure the use in human and animal health. Therefore, the studied plants should be used with caution. They present a relatively mild toxic potential, but it may be harmful to users if they are not properly utilized.

A sensitive RP-HPLC method with UV detection successfully measured phenol(s) in an ointment containing 3% Stryphnodendron adstringens extract. Chromatography used acetonitrile (0.05% trifluoroacetic acid):water (0.05% trifluoroacetic acid) (v/v), flow rate 0.8 mL min-1. Quantitation was accomplished by the external-standard method. Linearity for 2.00 to 16.00 μg mL-1 (gallic acid) and 1.14 to 18.24 μg mL-1 (gallocatechin) was established. Intra- and inter-day precision levels were under 5%. LOD and LOQ were 0.231 and 0.770 μg mL-1 (gallic acid) and 0.151 and 0.504 μg mL-1 (gallocatechin), respectively. Determination of phenols was unaffected by product excipients.

Um método de separação e quantificação por cromatografia líquida de alta eficiência (CLAE) em fase reversa foi desenvolvido usando água (0,05% de TFA):acetonitrila (0,05% de TFA) como fase móvel, em sistema gradiente para a análise dos flavan-3-óis presentes em extrato semipurificado das cascas de Stryphnodendron adstringens, Stryphnodendron polyphyllum e Stryphnodendron obovatum. A CLAE foi realizada com a fração acetato de etila (FAE) sendo submetida à extração em fase sólida (cartucho C18-E) com metanol:água (2:8), filtrada por membrana de porosidade 0,5 μm; a pré-coluna e coluna empregadas foram Phenomenex® Gemini C-18 (5 μm), com esta última mantida a 30 ºC, com vazão de 0,8 mL/min e detecção a 210 nm. Utilizaram-se soluções dos padrões de ácido gálico e galocatequina para a obtenção da curva analítica. O método proposto foi validado de acordo com a resolução RE n° 899/2003 da ANVISA. A análise quantitativa da FAE das três espécies mostrou que existe similaridade no teor de galocatequina. S. adstringens possui ácido gálico em uma proporção superior a 60% em relação às outras duas espécies. A metodologia desenvolvida mostrou-se viável à aplicação em plantas ricas em taninos, como nos casos de S. adstringens, S. polyphyllum e S. obovatum.A method of separation and quantification by reverse-phase high-performance liquid chromatography (HPLC) was developed, using water (0.05% TFA):acetonitrile (0.05% TFA) as the mobile phase in a gradient system. Flavan-3-ols present in a semipurified extract from the stem bark of Stryphnodendron adstringens, Stryphnodendron polyphyllum and Stryphnodendron obovatum were analyzed. The HPLC was performed with the ethyl-acetate fraction (EAF) using a solid-phase extraction on cartridges C18-E with methanol:water (2:8), filtered through a membrane of 0.5 μm pore size; the column was Phenomenex® Gemini C-18 (5 μm) at 30 ºC, with a flow rate of 0.8 mL/min. The analysis was done at 210 nm. Gallic acid and gallocatechin solutions were used as calibration standards. The proposed method was validated by resolution RE No. 899/2003 of the National Health Surveillance Agency. Quantitative analysis of the EAF showed high contents of flavan-3-ols in the stem bark of all three species. This study demonstrated that it is possible to determine the concentration of individual substances in tannin-rich plants. The system developed can be used as a chromatographic profile for the semipurified fraction of S. adstringens, S. polyphyllum, and S. obovatum.

Tuberculosis (TB) is a very serious problem worldwide and the increasing number of multiple drugs resistant TB cases makes the search for new anti-TB drugs an urgent need. Indigenous knowledge about the use of native plants to treat illnesses has contributed to the discovery of new medicines. In this study, the antimycobacterial activity of seven medicinal drinks was assessed: Ananas sativus (hydroalcoholic fruit extract), Aristolochia triangularis (aqueous and hydroalcoholic leaf, root and stem extracts), Bromelia antiacantha (hydroalcoholic fruit extract), Stryphnodendron adstringens (hydroalcoholic bark extract), Tabebuia ovellanedae (hydroalcoholic bark extract), Vernonia polyanthes (hydroalcoholic root extract), all used by the Vanuíre indigenous community in the treatment of respiratory diseases. The activity was evaluated by using a time-to-kill assay, in which Mycobacterium tuberculosis H37Rv was cultured on Löwenstein-Jensen medium, after thirty minutes, one, three, six, twelve and twenty-four hours contact of the bacteria with each drink. Within half to one hour contact, the hydroalcoholic drinks of A. triangularis, S. adstringens, T. ovellanedae and V. polyanthes reduced the bacterial growth by 2 orders of magnitude in CFU/mL, and all bacterial growth was absent after three hours contact. In contrast, no mycobactericidal effect was detected in the aqueous extract of A. triangularis or in the hydroalcoholic beverages of A. sativus and B. antiacantha, even after twenty-four hours contact. Keywords: Tuberculosis; indigenous drinks; Aristolochia triangularis; Stryphnodendron adstringens; Tabebuia ovellanedae; Vernonia polyanthes.

As normas oficiais para análise de sementes não estabelecem critérios para a execução de testes de germinação da maioria das espécies florestais. Assim, o objetivo deste trabalho foi a determinação do substrato, da temperatura e da necessidade de superação da dormência das sementes para o teste de germinação de Stryphnodendron adstringens. Foram avaliados o tratamento de escarificação com ácido sulfúrico por 60 min, os substratos vermiculita, rolo de papel, areia e solo e as temperaturas constantes de 20, 25, 30 e 35 ºC e alternadas de 15-35 ºC e 20-30 ºC, utilizando-se a primeira contagem de germinação (7 dias) e a porcentagem de plântulas normais, anormais, sementes mortas e dormentes (42º dia após a semeadura) com quatro repetições de 50 sementes. As sementes de S. adstringens devem ser submetidas ao teste de germinação, após superação da dormência, em substrato papel e nas temperaturas constantes de 25, 30 ou 35 ºC ou alternadas de 20-30 ºC.The official rules for seed testing don't establish criterions for accomplishment of germination test for greater number of forest species. Thus, the aim of this work was to define substrate, temperature and method for removing hard seededness for germination test of Stryphnodendron adstringens. They were studied: acid scarification with sulphuric acid for 60 minutes, substrates: vermiculita, rolled towel paper, sand and soil, and temperatures: constant, 20, 25, 30 and 35 ºC, and alternating, 15-35 ºC and 20-30 ºC. They were evalueted by first counting of the test (percentage of normal seedlings at 7 days after sowing), and the percentages of normal and abnormal seedlings, dormant and dead seeds (42 days after sowing), with four replications of 50 seeds. Seeds with hardness removed must be used to evaluate the germination of S. adstringens, in paper substrate and with constant temperature of 25, 30 or 35 ºC or alternating of 20-30 ºC.

O objetivo do trabalho foi identificar métodos para superar a dormência e promover a germinação de sementes de Stryphnodendron adstringens e Stryphnodendron polyphyllum. Sementes de ambas as espécies foram submetidas aos seguintes tratamentos desuperação de dormência: testemunha, escarificação mecânica (lixa número 220), H2O quente (temperatura inicial de 87oC) por 5 e por 15 minutos, escarificação química com H2SO4 (95%) por 30, 45, 60, 75, 90 e 105 minutos, choque térmico 1 (0oC e 50% UR por 1h e após imersão em água por 10 min. Ti=85oC Tf=57oC), choque térmico 2 (40oC e 40%UR por 6 h e após imersão em água por 10 min. Ti=0oC Tf=3oC), choque térmico 3 (40oC e 40% UR por 6h e após imersão em água por 10 min Ti=25oC Tf=25oC). Conclui-se que sementes pertencentes ao mesmo gênero, mas de espécies diferentes, respondem de forma diversa a alguns tratamentos de superação de dormência. Para ambas as espécies, a escarificação das sementes com H2SO4 (45 minutos) ou lixa são métodos que podem ser utilizados com máxima eficiência para a superação da dormência e promoção da germinação.The aim of this research was to identify methods for breaking dormancy and promoting germination of Stryphnodendron adstringens and S. polyphyllum seeds. Seeds of both species were submitted to the following treatments for breaking dormancy: control; mechanical scarification (sandpaper number 220); hot water (initial temperature of 87°C) for 5 and 15 minutes; chemical scarification with H2SO4 (95%) for 30, 45, 60, 75, 90 and 105 minutes; thermalshock 1 (0°C and 50% RH for 1 h and thereafter 10 minutes into water Ti=85°C, Tf=57°C); thermic shock 2 (40°C and 40% RH for 6 h and thereafter 10 minutes into water, Ti=0°C, Tf=3°C); thermal shock 3 (40°C and 40% RH for 6h and thereafter 10 minutes into water, Ti=25°C, Tf=25°C). We concluded that seeds belonging to samegenus but from different species answered differently to some treatments for breaking dormancy. Chemical scarification with H2SO4 (45 minutes) and mechanical scarification with sandpaper are methods that can be employed with efficiency to break dormancy and promote germination for both species.

O objetivo desta pesquisa foi determinar um protocolo de micropropagação para o barbatimão. Para indução de brotações, os segmentos nodais foram inoculados em meio de cultura MS com diferentes concentrações de cinetina (0, 1, 2, 3, 4 e 5mg L-1). Para indução de raízes, brotações obtidas "in vitro" foram inicialmente transferidas para meio MS contendo 50% da concentração de sais e com diferentes concentrações de ANA (0, 1, 2, 3 e 4mg L-1). Posteriormente, as brotações foram transferidas para meio MS contendo 50% da concentração de sais, suplementado com carvão ativado. Para a aclimatização, as plantas obtidas foram transferidas para tubetes contendo diferentes substratos e, então, envolvidas com saco plástico transparente para auxiliar temporariamente na manutenção da umidade. As concentrações de 1 e 5mg L-1 de cinetina foram as mais eficientes na indução de gemas e brotos, respectivamente. Brotações com maiores comprimentos foram observadas em meio de cultura suplementado com 1mg L-1 de cinetina. Raízes em maior número e comprimento foram observados em meio de cultura suplementado com 4mg L-1 de ANA. Os substratos utilizados não afetaram significativamente a taxa de sobrevivência das plantas durante a fase de aclimatização.The objective of the research was to determine a micropropagation protocol for the specie Stryphnodendron adstringens (Mart.) Coville. For shoot induction, nodal segments were inoculated in MS medium with different concentrations of kinetin (0, 1, 2, 3, 4 e 5mg L-1). For root induction, initially the shoots obtained 'in vitro' were transferred to MS containing 50% of salt concentration and with different concentrations of NAA (0, 1, 2, 3 e 4mg L-1). Lately, the shoots were moved to MS medium containing 50% of salt concentration, supplemented with activated charcoal. For acclimatization, the plants obtained were moved to plastic tubes containing different substrates and then covered with transparent plastic bags in order to maintain the environment humidity. The kinetin concentrations of 5.0 and 1.0mg L-1 were the most efficient for the induction of shots and buds, respectively. Shoot with a higher lengh were observed in MS medium supplemented with 1.0mg L-1 of kinetin. Roots in a higher number and length were observed in MS medium supplemented with 4.0mg L-1 of NAA. The substrates used had no significant effect on the rate of plant survival during the acclimatization stage.