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Nitrate, the form of nitrogen often associated with kelp growth, is typically low in summer during periods of high macroalgal growth. More ephemeral, regenerated forms of nitrogen, such as ammonium and urea, are much less studied as sources of nitrogen for kelps, despite the relatively high concentrations of regenerated nitrogen found in the Southern California Bight, where kelps are common. To assess how nitrogen uptake by kelps varies by species and nitrogen form in southern California, USA, we measured uptake rates of nitrate, ammonium, and urea by Macrocystis pyrifera and Eisenia arborea individuals from four regions characterized by differences in nitrogen availability-Orange County, San Pedro, eastern Santa Catalina Island, and western Santa Catalina Island-during the summers of 2021 and 2022. Seawater samples collected at each location showed that overall nitrogen availability was low, but ammonium and urea were often more abundant than nitrate. We also quantified the internal %nitrogen of each kelp blade collected, which was positively associated with ambient environmental nitrogen concentrations at the time of collection. We observed that both kelp species readily took up nitrate, ammonium, and urea, with M. pyrifera taking up nitrate and ammonium more efficiently than E. arborea. Urea uptake efficiency for both species increased as internal percent nitrogen decreased. Our results indicate that lesser-studied, more ephemeral forms of nitrogen can readily be taken up by these kelps, with possible upregulation of urea uptake as nitrogen availability declines.

Microglia, the resident immune cells of the central nervous system (CNS), play a crucial role in maintaining neural homeostasis but can also contribute to disease and injury when this state is disrupted or conversely play a pivotal role in neurorepair. One way that microglia exert their effects is through the secretion of small vesicles, microglia-derived exosomes (MGEVs). Exosomes facilitate intercellular communication through transported cargoes of proteins, lipids, RNA, and other bioactive molecules that can alter the behavior of the cells that internalize them. Under normal physiological conditions, MGEVs are essential to homeostasis, whereas the dysregulation of their production and/or alterations in their cargoes have been implicated in the pathogenesis of numerous neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), multiple sclerosis (MS), spinal cord injury (SCI), and traumatic brain injury (TBI). In contrast, MGEVs may also offer therapeutic potential by reversing inflammation or being amenable to engineering for the delivery of beneficial biologics or drugs. The effects of MGEVs are determined by the phenotypic state of the parent microglia. Exosomes from anti-inflammatory or pro-regenerative microglia support neurorepair and cell survival by delivering neurotrophic factors, anti-inflammatory mediators, and molecular chaperones. Further, MGEVs can also deliver components like mitochondrial DNA (mtDNA) and proteins to damaged neurons to enhance cellular metabolism and resilience. MGEVs derived from pro-inflammatory microglia can have detrimental effects on neural health. Their cargo often contains pro-inflammatory cytokines, molecules involved in oxidative stress, and neurotoxic proteins, which can exacerbate neuroinflammation, contribute to neuronal damage, and impair synaptic function, hindering neurorepair processes. The role of MGEVs in neurodegeneration and injury—whether beneficial or harmful—largely depends on how they modulate inflammation through the pro- and anti-inflammatory factors in their cargo, including cytokines and microRNAs. In addition, through the propagation of pathological proteins, such as amyloid-beta and alpha-synuclein, MGEVs can also contribute to disease progression in disorders such as AD and PD, or by the transfer of apoptotic or necrotic factors, they can induce neuron toxicity or trigger glial scarring during neurological injury. In this review, we have provided a comprehensive and up-to-date understanding of the molecular mechanisms underlying the multifaceted role of MGEVs in neurological injury and disease. In particular, the role that specific exosome cargoes play in various pathological conditions, either in disease progression or recovery, will be discussed. The therapeutic potential of MGEVs has been highlighted including potential engineering methodologies that have been employed to alter their cargoes or cell-selective targeting. Understanding the factors that influence the balance between beneficial and detrimental exosome signaling in the CNS is crucial for developing new therapeutic strategies for neurodegenerative diseases and neurotrauma. [ABSTRACT FROM AUTHOR]

The study of host–pathogen interactions has increased considerably in recent decades. This intercellular communication has been mediated by extracellular vesicles (EVs) that play an important role during the interaction. EVs are particles of lipid bilayer and described in different types of cells, eukaryotic or prokaryotic. Depending on their biogenesis they are described as exosomes (derived from multivesicular bodies) and microvesicles (derived from the plasma membrane). The EVs carry biomolecules, including nucleic acids, lipids, and proteins that can be released or internalized by other cells in different pathways (endocytosis, macropinocytosis, phagocytosis, or membrane fusion) in the process described as uptake. The balance between biogenesis and uptake of EVs could modify physiological and pathophysiological processes of the cell. This review is focusing on the dynamic roles of release and capture of EVs during host–pathogen interaction. We also do a critical analysis of methodologies for obtaining and analyzing EVs. Finally, we draw attention to critical points to be considered in EV biogenesis and uptake studies. [ABSTRACT FROM AUTHOR]

To perform an illocutionary act – e.g. to apologise – is it necessary, sufficient, or irrelevant that a hearer understand you to be performing that act? This issue is sometimes called the 'problem of uptake'. Famously, Austin's inaugural account makes uptake necessary for illocutionary force. For decades, critics have objected to this view: Some have sought to show that uptake is irrelevant; others have argued that uptake is important though not essential; while still others have argued that uptake is necessary though must be radically reframed. Nevertheless, it is widely agreed that there is a problem of uptake in Austin. In this paper, I contend that this view is wrong. Austinian uptake, I argue, is essential; yet, contra the standard reading, it is not synonymous with understanding. Uptake is a narrow criterion pertaining to conventional-linguistic competencies, which rules out specific kinds of misunderstanding, not misunderstanding überhaupt. The importation of non-Austinian assumptions about uptake from subsequent speech act theorists – specifically, the mis-equivocation of uptake with understanding a speaker's intention – I show, has muddied the water. This paper offers a novel reconsideration and salvaging of uptake and its significance, whose stakes I elucidate through intervention in the fraught issue of conceptualising sexual consent. [ABSTRACT FROM AUTHOR]

Hypothetical and experimental models of unbound fraction have been proposed to facilitate predicting the hepatic clearance (CL H) of drugs from values of intrinsic clearance for the unbound drug (CL int-in vitro-unbound) and the well-stirred model (WSM). The hypothetical model (fu -adjusted) is adjusting the unbound fractions determined in plasma in vitro to estimate the maximum unbound fractions at the hepatocytes if each drug-protein complex in plasma becomes fully dissociated at the membrane by any albumin (ALB)-facilitated hepatic uptake mechanism. The model of fu -adjusted is also adjusting the unbound fraction for a pH gradient effect across the membrane. Alternatively, the new experimental model (fu -dynamic) measures the unbound fractions resulting to the dynamic dissociation kinetics from proteins in the presence of plasma and a liver enzyme in an in vitro assay. The objective of this study was to conduct an in-depth analysis of previous CL H predictions made with these unbound fractions in a companion manuscript. Furthermore, a new dataset on transporter substrates was also included in this study. Finally, the physiological basis of fu -adjusted has been redefined to extend its applicability with more drugs. In this case, there are lower concentrations of binding proteins in liver versus plasma that could also explain the higher unbound fractions for that organ. The outcomes associated to additional analyses pointed out that fu -adjusted , again, generally provided the most accurate predictions of CL H because fu -dynamic has generated superior biases of underpredictions or overpredictions. For slowly metabolized drugs bound to ALB, fu -dynamic was definitively less accurate than fu -adjusted. For other drug properties, fu -dynamic fared better but it was still not generally more accurate than fu -adjusted. Furthermore, experimental values of fu -dynamic were sometimes incoherent. For example, drugs bound to alpha-acid glycoprotein (AGP) did not follow the principle of fu -dynamic (i.e. , values of fu -dynamic did not correlate with values of CL int-in vitro-unbound) by contrast to those drugs bound to ALB. Therefore, the current experimental setting for fu -dynamic might be unsuitable in some circumstances. Overall, this study confirmed that calculated values of fu -adjusted were as accurate as experimental values of fu -dynamic and can even be more accurate. A guidance on which unbound fraction to use in the WSM is also provided. [ABSTRACT FROM AUTHOR]

As an act of self-disclosure, the speech act of self-praise may be perceived as problematic or inappropriate due to societal expectations, such as politeness preferences. Despite recent growing interest in self-praise, it remains relatively under-researched, particularly in naturally occurring conversation in languages other than English. An examination of 53 hours of Japanese conversation (both telephone and face-to-face) reveals that despite Japanese societal norms against self-praise, speakers do praise themselves, and that this behavior occurs in interactions within both close relationships (i.e., among friends and family members) and distant ones (i.e., in first meetings). However, its relative infrequency indicates that self-praise is a marked social behavior in Japanese conversation. The data also demonstrate some distinctive patterns in the ways in which self-praise is delivered and received depending on the type of conversation. Interactional patterns that emerge in the data illustrate the integral role that coparticipants (i.e., the recipients of speakers' self-praise) play in making this risky social act possible and appropriate in conversation. • Self-praise is more marked in conversation than in online discourse. • Self-praise is delivered and received differently depending on conversation type. • Participants work together to minimize the illocutionary effects of self-praise. [ABSTRACT FROM AUTHOR]

Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used as a natural fungicide to control plant diseases, particularly for managing rice blast. However, its uptake mechanism and transport in rice remain to be explored. In this article, rice seedlings were treated by the foliar spraying method, and the content of KSM in rice leaves, stems and roots under different treatments was detected by high‐performance liquid chromatography tandem mass spectrometry (HPLC‐MS/MS), respectively. Results showed that leaf‐applied KSM could be transported to the phloem and migrate to roots and stems after uptake by leaves. Concentration, temperature and pH had significant effects on the uptake of KSM. Compared with the control, the competitive inhibitors d‐glucose and phlorizin both inhibited the uptake of KSM, demonstrating that sugar transporter proteins were involved in the uptake process. The energy inhibitors dinitrophenol (DNP) and carbonyl cyanide chlorophenylhydrazone (CCCP) also significantly inhibited the uptake of KSM, indicating that the uptake of KSM required energy consumption. Thus, the uptake of KSM by rice was an active process involving sugar transporter proteins, and it could migrate downward through the phloem. This study contributes to the promotion of the scientific application of antibiotics and the biological control of crop diseases. It will also provide a theoretical basis for the development of root‐targeted pesticides and transport pesticides with phloem mobility. [ABSTRACT FROM AUTHOR]

Soybean (Glycine max [L.] Merr.), one of the top 10 crops produced worldwide, is a significant dietary source of isoflavones. Understanding the bioaccessibility and bioavailability of soy isoflavones (SIs) is important fgor comprehending their various bioactivities in the human body. In vitro approaches can provide valuable insights into the bioavailability of SIs across food matrices and their step-by-step digestive mechanisms. This review focused on evaluating reports that employ invitro methods to study the bioavailability of SIs, using human-simulated gastrointestinal digestive system models and the Transwell system with Caco-2 cell monolayers. Specifically, this review discusses how the molecular structures of SIs influence their bioaccessibility and bioavailability. The differences in bioaccessibility and bioavailability of SI aglycones and their conjugated SIs indicate that the molecular structure is a critical factor influencing the bioactivities of SIs. This review provides important information for evaluating and understanding the physiological effects of soybean or SI-containing foods. [ABSTRACT FROM AUTHOR]

Fagopyrum dibotrys is a herbal plant. Selenium (Se) is a beneficial element for plants; selenium nanoparticles (SeNPs) are gaining importance in food and agriculture due to their low toxicity and high activity. This study revealed that foliar application of SeNPs enhanced superoxide dismutase, glutathione peroxidase, and peroxisome activities and significantly enhanced the flavonoid compound content in F. dibotrys. SeNPs with a concentration of 5.0 mg L−1 also promoted the growth of F. dibotrys. The foliar application of SeNPs could be absorbed by pores in leaves of F. dibotrys and mainly transformed to selenomethionine (32.5–43.2%) and selenocysteine (23.4–38.4%) in leaves and tubers of F. dibotrys. Consequently, this study offers a profound understanding of plants' uptake and biotransformation of SeNPs. Furthermore, the findings of this study have suggested that SeNPs can be applied to improve the quantity and quality of the herbal plant of F. dibotrys. [ABSTRACT FROM AUTHOR]

In this paper I characterize an unexplored category of what I call unfinished speech acts (USA), intentionally designed by the speaker as incomplete, and intended to be finalised by the hearer. The speaker relies on the hearer’s contribution because she is willing to minimize conversational risk regarding a certain implicit content or a certain indirect speech act. I focus on three paradigm cases: insinuations, polite novel (as opposed to conventionalized) indirect speech acts, and flirting. I sketch a general characterization of USAs, and underline several important dissimilarities between USAs and standard implicatures and conventionalized indirect speech acts. In closing, I gesture at ways the notion of USA could clarify recent philosophical debates, from both a theoretical and a socially engaged point of view: my proposal elucidates how explicit and implicit contents manage to enter the conversational context and, more generally, how speech acts are successfully performed – with an obvious impact on how we conceptualize both the speaker’s and the audience’s conversational responsibility. [ABSTRACT FROM AUTHOR]

The application of nanotechnology in agriculture has emerged as a tool of great sustainable use for crop improvement. Pregerminative treatments with various chemicals and hormones are routine procedures to break the seeds' dormancy and stimulate crop germination. However, innovative techniques using nanoparticles (NPs) for seed priming are considered a potentially effective and efficient strategy to promote seed germination, nutrient delivery, and growth through impregnation. The present work aimed to synthesize iron oxide NPs (FeONPs) by a green method by using vegetal organic waste as a source of reducing and stabilizer agents for NPs formation, and then it was used to promote seed germination, growth, and biofortification of Solanum lycopersicum. The results indicate the effectiveness of orange peel waste for the one-step green synthesis of magnetite Fe3O4 NPs with an apparent spherical form and an average size of 13 nm determined with SEM analysis. The application of NPs as a nano priming (0.1–300 ppm) did not show significant changes in the germination development of tomatoes. However, positive effects on the growth of seedlings were observed with medium doses administered (10–50 ppm) because root length, stem length, and biomass were increased. In addition, an increase in the Fe and other micronutrients of both grain and seedlings was also observed, suggesting that nano priming with Fe3O4 NPs can stimulate the growth and Fe uptake of S. lycopersicum crops. [ABSTRACT FROM AUTHOR]

AbstractProviding corrective feedback to second language (L2) writing constitutes a crucial digital affordance for AI-assisted writing systems. However, L2 writers’ revision strategies and obstacles to adopting AI-generated feedback, such as ChatGPT, remain unclear. Forty-five L2 students in a computer science program were tasked with seeking corrective feedback from ChatGPT for their argumentative essays, followed by an analysis of their revisions and rationale for feedback uptake strategies. The findings revealed that approximately 38% of the feedback was either explicitly argued (22%) or ignored (16%). Upon controlling for writing proficiency, participants statistically rejected a significantly higher proportion of feedback at the content level (e.g. evidence) than at the form level (e.g. grammar). Utilizing the Technology Acceptance Model, the reasons for rejecting or ignoring ChatGPT-generated feedback were examined through participants’ reflective data, focusing on two perspectives: inconvenience to use and unusefulness. Inconvenient factors included (1) overload feedback, (2) provision of general descriptions instead of specific error highlighting, and (3) repetitive and tedious comments. Themes related to unusefulness encompassed (1) misinterpretation of authors’ intentions, (2) lack of clarity and illustrative examples, and (3) extraneous and irrelevant feedback. The implications entail pedagogical strategies to mitigate barriers and foster feedback literacy in AI-assisted educational environment. [ABSTRACT FROM AUTHOR]

Despite evidence supporting genetic testing's utility in hereditary cancer risk management, uptake remains low among at-risk relatives of a hereditary cancer patient. The qualitative systematic review aims to identify the psychosocial barriers and facilitators associated with the uptake of genetic counselling services and/or genetic testing (GC/GT). A systematic literature search was performed across six databases in June 2023, limited to studies published in English from 2010 onwards. Qualitative studies interviewing hereditary cancer patients, their relatives, and/or healthcare providers to identify the psychosocial barriers and facilitators associated with the uptake of genetic testing for hereditary cancer syndrome were eligible for inclusion. A thematic analysis was conducted on the extracted data from 41 eligible qualitative studies. 54% of the studies were from the United States and 75% of the participants were female. 14 psychosocial barriers and nine facilitators to the uptake of genetic testing services were identified. The most frequently cited psychosocial barriers to genetic testing uptake were ‘emotional roller coaster’, ‘threat of genetic discrimination’, and ‘no perceived benefit of genetic testing’. In contrast, the most common facilitators were ‘concern for family’, ‘easing personal worries’, and ‘knowledge is empowering’. Our findings reveal complex factors affecting GC/GT service uptake, some with dual effects. [ABSTRACT FROM AUTHOR]

Caring for a disabled child involves both indirect and direct economic costs, which in turn may create a financial burden for the family. Using Norway as a case, we examine if public compensatory measures reach those most in need, by assessing whether socioeconomic disadvantages and immigrant background impact adversely on the uptake of a nationally available cash benefits eligible for parents caring for a child with a disability. We take advantage of national administrative register data on children with a selection of well‐defined diagnoses born between 2009 and 2017 (N = 14,469). Our results show nonnegligible, but relatively minor, social inequalities in the uptake of cash benefits related to socioeconomic and immigrant background. Our findings indicate that this state's financial support seems to reach those most in need. However, it is essential to deepen our understanding of how socioeconomic and immigrant backgrounds influence access to and utilization of municipal healthcare services. Notably, the application process for these services often involves a greater degree of discretion on the part of welfare professionals. Further research is needed to illuminate the nuanced dynamics of these factors in the context of accessing healthcare services at the local level. [ABSTRACT FROM AUTHOR]

Background: Cervical cancer is a leading cause of cancer mortality globally, especially in Africa, including Ethiopia. This review assesses predictors of cervical cancer screening uptake among Ethiopian-eligible women using the Health Belief Model. Higher education levels, perceived susceptibility, severity, and fewer barriers are associated with increased screening. Effective HBM-based interventions could enhance screening rates, potentially reducing cervical cancer incidence and mortality. Objective: The review aimed to synthesize the existing literature on the prevalence of Cervical Cancer Screening Service Uptake and Associated Factors among Eligible Women using the Health belief model in Ethiopia, 2024. Method: This systematic review and meta-analysis searched Google Scholar, PubMed, and the Cochrane Library engine. Key terms such as "Cervical cancer screening", "uptake", "utilization", "factors", "barriers", and "Ethiopia" were used to identify relevant articles. Data extraction utilized a detailed form, and the methodological quality of each study was assessed using the JBI quality appraisal checklist for cross-sectional studies. Statistical analysis was conducted using STATA version 17, and the meta-analysis findings were presented using forest plots and tables. Result: The result of seven studies revealed that the overall prevalence of Cervical Cancer Screening Service Uptake among eligible women in Ethiopia was 21% (95% CI: 15%-27%). Factors independently associated with Cancer Screening Service Uptake included: Knowledge (OR = 4.563, 95% CI: 1.012–4.188), age 30 up to 49 (OR = 4.106, 95% CI: 1.562–6.650), history of STD (OR = 2.59, 95% CI: 1.694–4.486), high perceived susceptibility (OR = 3.814, 95% CI: 2.312–5.316), high perceived severity (OR = 2.603, 95% CI: 2.203–3.003), low perceived barrier (OR = 4.390, 95% CI: 1.331–8.449), high perceived self-efficacy (OR = 4.77, 95% CI: 4.102–5.431), high perceived benefit (OR = 3.67, 95% CI: 1.851–5.489), and education level greater than primary level (OR = 4.497, 95% CI: 3.619–5.375). Conclusion: Cervical cancer is a major public health challenge in Ethiopia. Consequently, there is a pressing need for the governments to formulate comprehensive, multi-sectorial policies and strategies. These initiatives should be designed to address the problem influenced by interconnected factors, to reduce the prevalence of cervical cancer in Ethiopia. [ABSTRACT FROM AUTHOR]

Background: Breast cancer is the most common cancer among women worldwide. Breast cancer screening programs are widely promoted because of their effectiveness in the early detection of cancer. However, a significant proportion of eligible Catholic nuns in the Lake Zone of Tanzania remain underscreened. This study is aimed at investigating the factors associated with breast cancer screening uptake among Catholic nuns in Lake Zone, Tanzania. Methods: This was a cross‐sectional study design among 385 catholic nuns. Simple random sampling was deployed to enrolled catholic nuns, the ODK collect v2023.2.4 was electronically used to collect data. Binary logistic regression was used to assess the factors associated with the uptake of breast cancer screening. Statistical analysis was performed using STATA 18.0, with a significance level set at a p value less than 0.05. Result: The prevalence uptake of breast cancer screening (i.e., breast self‐examination, clinical examination, or mammography examination) was 64% (n = 247, 95% CI, 59.3%–68.9%). A total of 57.4% had inadequate knowledge on the uptake of breast cancer screening (n = 221, 95% CI, 52.4%–62.4%). Also, the study found that 61.3% (95% CI, 56.4%–66.2%) of Catholic Nuns have negative attitudes towards the uptake of self‐breast examination among Catholic nuns. The findings revealed that 55.6% (n = 133, 95% CI, 50.6%–60.6%) and 52.7% (n = 52.7%, 95% CI, 47.7%–57.7%) of Catholic nuns negatively accept breast cancer screening and self‐breast examination, respectively. Nuns aged above 60 years were less likely to not perform BCS with a COR of 0.62 (95% CI, 0.39–0.97). Also, Catholic nuns who are in the nonhealth field are more likely to not perform BCS with a COR of 1.71 (95%, 1.07–2.74). Likewise, Catholic nuns who had negative acceptability of the Self‐breast examination were more likely to not perform BCS with an AOR of 1.65 (95% CI, 1.07–2.55). Conclusion: A study found a low uptake of breast cancer screening among Catholic nuns. This highlights the need for breast health intervention programs within religious congregations to address misconceptions and promote early detection. [ABSTRACT FROM AUTHOR]

Biotic ligand models (BLMs) predict the toxicity of metals in aquatic environments by accounting for metal interactions with cell surface receptors (biotic ligands) in organisms, including water chemistry (metal speciation) and competing cations. Metal binding constants (log KMBL values), which indicate the affinity of metals for cell surface receptors, are fundamental to BLMs, but have only been reported for a few commonly investigated metals and freshwater species. This review evaluated literature toxicity and uptake data for seven key metals (cadmium (Cd), cobalt (Co), copper (Cu), nickel (Ni), lead (Pb), uranium (U), and zinc (Zn)) and four key competing cations (protons (H), calcium (Ca), magnesium (Mg), and sodium (Na)), to derive average metal binding constants for freshwater organisms/taxa. These constants will improve current BLMs for Cd, Cu, Ni, Pb, and Zn, and aid in developing new BLMs for Co and U. The derived metal binding constants accurately predicted metal toxicity for a wide range of freshwater organisms (75–88% of data were within a factor of two and 88–98% of data were within a factor of three of the ideal 1:1 agreement line), when considering metal speciation, competing cations and the fraction of cell receptors ((fC)M50%) occupied by the metal at the median (50%) effect concentration (EC50). For many organisms, toxicity occurs when 50% of cell surface receptors are occupied by the metal, though this threshold can vary. Some organisms exhibit toxicity with less than 50% receptor occupancy, while others with protective mechanisms show reduced toxicity, even with similar log KMBL values. For Cu, U, and Pb, the toxic effect of the metal hydroxide (as MOH+) must be considered in addition to the free metal ion (M2+), as these metals hydrolyse in circumneutral freshwaters (pH 5.5 to 8.5), contributing to toxicity. [ABSTRACT FROM AUTHOR]

Background: The uptake of research findings into clinical practice is critical to providing health care that improves health outcomes for patients. This study explored how Patient-Centered Outcomes Research Institute (PCORI) awardees perceive the relationship between engagement of patients and other partners in research and three uses, or applications, of patient-centered comparative clinical effectiveness research (CER) study findings, which may lead to uptake in clinical practice: (1) Integration into clinical practice guidelines, recognized point-of-care decision tools, or documents that may inform policy; (2) Implementation beyond the study, including at sites outside of the study setting or patient populations; and (3) Active dissemination of findings to specific audiences by parties external to the study team. Methods: This exploratory qualitative study examined awardee and partner perceptions of what led to each use of study findings and how engaged partners contributed. We purposively selected PCORI-funded research projects with documentation of each use and conducted virtual interviews with 42 individuals (15 PIs or project leads, 2 research team members, and 25 partners) from 17 projects. We conducted thematic analysis of individual projects or project sets, across projects within each use case, and across the three uses. Results: Participants described three primary activities in which engaged partners made contributions before, during and after CER studies that facilitated the use of study findings: (1) generating relevant study findings, (2) distributing study findings strategically, and (3) making connections to people or organizations outside the study team. In addition, engagement continued to facilitate the use of study findings during subsequent PCORI-funded implementation and dissemination-specific projects, with partners adapting interventions and creating and tailoring dissemination messages and products. Finally, participants described attributes of teams' engagement approaches that may have supported partner contributions, including early and ongoing engagement, leveraging partners' connections and understanding of community needs, and using multiple engagement approaches. Conclusion: This study identified examples of how engagement can help facilitate the use of CER study findings, especially when engagement contributions occur in meaningful ways. Findings from this study suggest a framework for future research on the relationship between engagement in research and uptake of study findings into clinical practice. Plain English summary: This study looked at how PCORI awardees describe the relationship between engagement in research and three uses of study findings: (1) Citing findings in clinical practice guidelines or other documents that support health policy; (2) Putting findings into practice after the study; and (3) Sharing findings with specific audiences by people not on the study team. We selected PCORI-funded research projects that had signs of each use. Then, we interviewed 42 people, including 15 lead investigators or project leads, 2 research team members, and 25 partners. We summarized findings for each project and then across the three uses. Partners helped move forward each of the three uses of study findings. They helped to plan and conduct the studies, making findings relevant. Partners also helped to plan and then share study findings. They made connections to people and organizations for the use of study findings. In addition, partners helped to put findings into practice after the study and tailored and shared findings in their communities. Finally, the way project teams engaged partners may have supported partner contributions. Project teams had early and ongoing engagement in their study. Partners shared their understanding of community needs and used their connections to share findings. Also, projects often had more than one way to work with partners. Findings from this study highlight ways engagement in research can contribute to use and potentially uptake of study findings in clinical practice. The study also suggests topics for future research on how engagement affects the use of findings. Manuscript Text. [ABSTRACT FROM AUTHOR]

Microplastics, particularly those in the micrometer scale, have been shown to enter the human body through ingestion, inhalation, and dermal contact. Recent research indicates that microplastics can potentially impact the central nervous system (CNS) by crossing the blood-brain barrier (BBB). However, the exact mechanisms of their transport, uptake, and subsequent toxicity at BBB remain unclear. In this study, we evaluated the size-dependent uptake and cytotoxicity of polystyrene microparticles using an engineered BBB model. Our findings demonstrate that 0.2 μm polystyrene microparticles exhibit significantly higher uptake and transendothelial transport compared to 1.0 μm polystyrene microparticles, leading to increased permeability and cellular damage. After 24 h of exposure, permeability increased by 15.6-fold for the 0.2 μm particles and 2-fold for the 1.0 μm particles compared to the control. After 72 h of exposure, permeability further increased by 27.3-fold for the 0.2 μm particles and a 4.5-fold for the 1.0 μm particles compared to the control. Notably, microplastics administration following TNF-α treatment resulted in enhanced absorption and greater BBB damage compared to non-stimulated conditions. Additionally, the size-dependent toxicity observed differently between 2D cultured cells and 3D BBB models, highlighting the importance of testing models in evaluating environmental toxicity. [ABSTRACT FROM AUTHOR]

Predicting the response of aquatic species to environmental contaminants is challenging, in part because of the diverse biological traits within communities that influence their uptake and transfer of contaminants. Nanoplastics are a contaminant of growing concern, and previous research has documented their uptake and transfer in aquatic food webs. Employing an established method of nanoplastic tracking using metal-doped plastics, we studied the influence of biological traits on the uptake of nanoplastic from water and diet in freshwater predators through two exposure assays. We focused on backswimmers (Anisops wakefieldi) and damselfly larvae (Xanthocnemis zealandica) - two freshwater macroinvertebrates with contrasting physiological and morphological traits related to feeding and respiration strategies. Our findings reveal striking differences in nanoplastic transfer dynamics: damselfly larvae accumulated nanoplastics from water and diet and then efficiently eliminated 92% of nanoplastic after five days of depuration. In contrast, backswimmers did not accumulate nanoplastic from either source. Differences in nanoplastic transfer dynamics may be explained by the contrasting physiological and morphological traits of these organisms. Overall, our results highlight the importance and potential of considering biological traits in predicting transfer of nanoplastics through aquatic food webs. [ABSTRACT FROM AUTHOR]

Atherosclerotic lesions mainly form in arterial areas exposed to low shear stress (LSS), where endothelial cells express a senescent and inflammatory phenotype. Conversely, areas exposed to high shear stress (HSS) are protected from plaque development. Endothelial extracellular vesicles (EVs) have been shown to regulate inflammation and senescence, and therefore play a crucial role in vascular homeostasis. Whilst previous studies have shown links between hemodynamic forces and EV release, the effects of shear stress on the release and uptake of endothelial EVs remains elusive. We aim to decipher the interplay between these processes in endothelial cells exposed to atheroprone or atheroprotective shear stress. Confluent HUVECs were exposed to LSS or HSS for 24 h. Large and small EVs were isolated from conditioned medium by centrifugation and size exclusion chromatography. They were characterised by TEM, Western blot, tunable resistive pulse sensing, flow cytometry and proteomics. Uptake experiments were performed using fluorescently‐labelled EVs and differences between groups were assessed by flow cytometry and confocal microscopy. We found that levels of large and small EVs in conditioned media were fifty and five times higher in HSS than in LSS conditions, respectively. In vivo and in vitro uptake experiments revealed greater EV incorporation by cells exposed to LSS conditions. Additionally, endothelial LSS‐EVs have a greater affinity for HUVECs than HSS‐EVs or EVs derived from platelets, erythrocytes and leukocytes. Proteomic analysis revealed that LSS‐EVs were enriched in adhesion proteins (PECAM1, MCAM), participating in EV uptake by endothelial cells. LSS‐EVs also carried mitochondrial material, which may be implicated in elevating ROS levels in recipient cells. These findings suggest that shear stress influences EV biogenesis and uptake. Given the major role of EVs and shear stress in vascular health, deciphering the relation between these processes may yield innovative strategies for the early detection and treatment of endothelial dysfunction. [ABSTRACT FROM AUTHOR]

The application of biosolids, manure, and slurry onto agricultural soils and the growing use of treated wastewater in agriculture result in the introduction of human and veterinary pharmaceuticals to the environment. Once in the soil environment, pharmaceuticals may be taken up by crops, resulting in consequent human exposure to pharmaceutical residues. The potential side effects of pharmaceuticals administered in human medicine are widely documented; however, far less is known regarding the risks that arise from incidental dietary exposure. The aim of this study was to evaluate human exposure to pharmaceutical residues in crops and assess the associated risk to health for a range of pharmaceuticals frequently detected in soils. Estimated concentrations of carbamazepine, oxytetracycline, sulfamethoxazole, trimethoprim, and tetracycline in soil were used in conjunction with plant uptake and crop consumption data to estimate daily exposures to each compound. Exposure concentrations were compared to Acceptable Daily Intakes (ADIs) to determine the level of risk. Generally, exposure concentrations were lower than ADIs. The exceptions were carbamazepine, and trimethoprim and sulfamethoxazole under conservative, worst-case scenarios, where a potential risk to human health was predicted. Future research therefore needs to prioritize investigation into the health effects following exposure to these compounds from consumption of contaminated crops. [ABSTRACT FROM AUTHOR]

The use of zinc and Sulphur solubilizing bacteria to improve compost will help in the mineralization of these vital elements during its production process. Hence, the objective of this paper was to evaluate the assessment of the ability of some plant growth promoting bacteria to solubilize zinc and sulphur and hydrolyze starch and lipid and degrade cellulose in compost using appropriate standard techniques. Data obtained show out of 15 isolates screened for zinc and Sulphur solubilizing ability, only five were able to solubilize zinc and Sulphur as well as hydrolyze starch, lipid and degrade cellulose. The five isolates were selected and identified as Enterobacter kobei, Thiobacillus sp., Aspergillus udagawae, Aspergillus terreus and Meyerozyma guillermondii. Meyerozyma guillermondii showed the greatest ability to solubilize zinc and sulphur with solubilisation efficiency of 4.6 and 4.4, respectively. The five selected isolates showed great potential for possible use to fortify compost as a way of enriching the compost with zinc and sulphur for plant uptake. [ABSTRACT FROM AUTHOR]

Purpose: Seasonal influenza epidemics accounted for significant morbidity and mortality loads worldwide despite the availability of a safe vaccine as an efficient tool against severity of the disease. However, the uptake of the latter was sub-optimal. This study aims to identify predictors and barriers related to seasonal influenza vaccine uptake in the Kingdom of Bahrain. Design/methodology/approach: A cross-sectional study enrolled 502 individuals attending primary healthcare centers in Bahrain for ambulatory care between July and August 2022. The data were collected using an interviews-based questionnaire which included questions on demographic data, knowledge and attitudes and practices toward influenza vaccine. The authors identified the barriers as well as the determinants of the vaccine uptake and its recommendation to others. Findings: The mean age of participants was 35.07 years (SD = 13.9). Most of the respondents were Bahraini (86.5%) and 53.4 % were females. The results revealed that 34.1% have previous information about the influenza vaccine and 36.9% versus 69.9% are willing to receive the vaccine or advice it to others, respectively. Determinants of vaccine uptake were identified. Originality/value: This study confirmed a sub-optimal influenza vaccine acceptance in the general community of Bahrain despite a global access in primary care. Health professionals need to be more proactive in mobilizing the community and particularly females toward influenza vaccination. [ABSTRACT FROM AUTHOR]

Anthropogenic pollution of the aquatic environment with microplastics is one of the most urgent but least studied problems of modern ecotoxicology. This review, based on recent literature data, provides an analysis of studies in the field of absorption, bioaccumulation, and biological effects of microplastic exposure in freshwater bivalve mollusks (Bivalvia). A total of 22 studies have been conducted so far, which were performed on representatives of three families: Cyrenidae (45), Unionidae (25), and Dreissenidae (30%). The studies are made up of field observations (43.5) and field (8.7) and laboratory (47.8%) experiments. It has been shown that freshwater bivalves, as active filters, are able to absorb and accumulate in soft tissues (gills and hepatopancreas) microplastic particles from both water and bottom sediments, perceiving them as food objects. The bioaccumulation of microplastics in mollusks leads to functional and structural disorders in the body. The joint action of microplastics and other pollutants (cadmium, polychlorinated biphenyls, and pharmacological drugs) can cause both synergistic and antagonistic effects in the biological responses of mollusks. Based on these studies, it is suggested to use bivalve mollusks as bioindicators of freshwater pollution with microplastics. [ABSTRACT FROM AUTHOR]

The assessment of residue, absorption, conduction, and degradation of agricultural organosilicon surfactants in the environment is hindered by the lack of information on active ingredients and corresponding quantitative standards for organosilicon spray adjuvants. The spray adjuvant 'Jiexiaoli,' a primary organosilicon spray agent in China, was identified as hydroxy (polyethylene) propyl‐heptamethyl trisiloxane (TSS‐H) with 3–15 ethoxy (EO) groups. Purification of TSS‐H was achieved through semi‐preparative separation using high‐performance liquid chromatography (HPLC), resulting in TSS‐H purity exceeding 96%. An accurate residual detection method for nine oligomers (4–12 EO) of TSS‐H in rice roots, stems, leaves, and culture solution samples was developed using HPLC tandem high‐resolution mass spectrometry (HPLC‐HRMS). Recoveries for nine oligomers of TSS‐H in the four matrices ranged from 80.22% to 104.01%. Foliar application experiments demonstrated that TSS‐H did not transfer from the upper to the lower parts of the rice plant. The half‐lives of each oligomer (4–12 EO) in leaves were less than 3.21 days. Root application experiments revealed a root concentration factor (RCF) ranging from 0.20 to 0.56, a biological enrichment factor (BCF) ranging from 0.36 to 0.68, a transpiration factor (TSCF) ranging from 0.069 to 0.086, and a transport factor (TF) ranging from 0.08 to 0.43. These results indicated that TSS‐H could be absorbed by rice roots and conducted to the above‐ground parts of rice plants. This study fills the data gap in the environmental risk and food safety assessment of agricultural silicone spray adjuvants. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Abstract Background Cervical cancer is a leading cause of cancer mortality globally, especially in Africa, including Ethiopia. This review assesses predictors of cervical cancer screening uptake among Ethiopian-eligible women using the Health Belief Model. Higher education levels, perceived susceptibility, severity, and fewer barriers are associated with increased screening. Effective HBM-based interventions could enhance screening rates, potentially reducing cervical cancer incidence and mortality. Objective The review aimed to synthesize the existing literature on the prevalence of Cervical Cancer Screening Service Uptake and Associated Factors among Eligible Women using the Health belief model in Ethiopia, 2024. Method This systematic review and meta-analysis searched Google Scholar, PubMed, and the Cochrane Library engine. Key terms such as "Cervical cancer screening", "uptake", "utilization", "factors", "barriers", and "Ethiopia" were used to identify relevant articles. Data extraction utilized a detailed form, and the methodological quality of each study was assessed using the JBI quality appraisal checklist for cross-sectional studies. Statistical analysis was conducted using STATA version 17, and the meta-analysis findings were presented using forest plots and tables. Result The result of seven studies revealed that the overall prevalence of Cervical Cancer Screening Service Uptake among eligible women in Ethiopia was 21% (95% CI: 15%-27%). Factors independently associated with Cancer Screening Service Uptake included: Knowledge (OR = 4.563, 95% CI: 1.012–4.188), age 30 up to 49 (OR = 4.106, 95% CI: 1.562–6.650), history of STD (OR = 2.59, 95% CI: 1.694–4.486), high perceived susceptibility (OR = 3.814, 95% CI: 2.312–5.316), high perceived severity (OR = 2.603, 95% CI: 2.203–3.003), low perceived barrier (OR = 4.390, 95% CI: 1.331–8.449), high perceived self-efficacy (OR = 4.77, 95% CI: 4.102–5.431), high perceived benefit (OR = 3.67, 95% CI: 1.851–5.489), and education level greater than primary level (OR = 4.497, 95% CI: 3.619–5.375). Conclusion Cervical cancer is a major public health challenge in Ethiopia. Consequently, there is a pressing need for the governments to formulate comprehensive, multi-sectorial policies and strategies. These initiatives should be designed to address the problem influenced by interconnected factors, to reduce the prevalence of cervical cancer in Ethiopia.

Abstract Microplastics, particularly those in the micrometer scale, have been shown to enter the human body through ingestion, inhalation, and dermal contact. Recent research indicates that microplastics can potentially impact the central nervous system (CNS) by crossing the blood-brain barrier (BBB). However, the exact mechanisms of their transport, uptake, and subsequent toxicity at BBB remain unclear. In this study, we evaluated the size-dependent uptake and cytotoxicity of polystyrene microparticles using an engineered BBB model. Our findings demonstrate that 0.2 μm polystyrene microparticles exhibit significantly higher uptake and transendothelial transport compared to 1.0 μm polystyrene microparticles, leading to increased permeability and cellular damage. After 24 h of exposure, permeability increased by 15.6-fold for the 0.2 μm particles and 2-fold for the 1.0 μm particles compared to the control. After 72 h of exposure, permeability further increased by 27.3-fold for the 0.2 μm particles and a 4.5-fold for the 1.0 μm particles compared to the control. Notably, microplastics administration following TNF-α treatment resulted in enhanced absorption and greater BBB damage compared to non-stimulated conditions. Additionally, the size-dependent toxicity observed differently between 2D cultured cells and 3D BBB models, highlighting the importance of testing models in evaluating environmental toxicity. Graphical Abstract

Abstract Predicting the response of aquatic species to environmental contaminants is challenging, in part because of the diverse biological traits within communities that influence their uptake and transfer of contaminants. Nanoplastics are a contaminant of growing concern, and previous research has documented their uptake and transfer in aquatic food webs. Employing an established method of nanoplastic tracking using metal-doped plastics, we studied the influence of biological traits on the uptake of nanoplastic from water and diet in freshwater predators through two exposure assays. We focused on backswimmers (Anisops wakefieldi) and damselfly larvae (Xanthocnemis zealandica) - two freshwater macroinvertebrates with contrasting physiological and morphological traits related to feeding and respiration strategies. Our findings reveal striking differences in nanoplastic transfer dynamics: damselfly larvae accumulated nanoplastics from water and diet and then efficiently eliminated 92% of nanoplastic after five days of depuration. In contrast, backswimmers did not accumulate nanoplastic from either source. Differences in nanoplastic transfer dynamics may be explained by the contrasting physiological and morphological traits of these organisms. Overall, our results highlight the importance and potential of considering biological traits in predicting transfer of nanoplastics through aquatic food webs.

Abstract Background The Chinese biosimilar industry has demonstrated rapid growth in recent years. Limited evidence is available about biosimilar uptake at the national level. This study aimed to assess biosimilar uptake in the case of trastuzumab and to explore potential factors influencing the biosimilar penetration at national and provincial levels. Methods This study employed an interrupted time series analysis to assess the level and trend changes of national trastuzumab originator consumption and the overall trastuzumab consumption after the price reduction of the originator and the introduction of the first biosimilar using the China Hospital Pharmacy Audit procurement data from March 2018 to February 2023. A latent class trajectory model (LCTM) was also adopted to estimate the biosimilar penetration across 30 provincial-level administrative divisions (PLADs). Based on the LCTM grouping results, provincial characteristics were analyzed. Results After rapid growth, the penetration of biosimilars demonstrated a moderate ascending trend at the national level, reaching 27% in February 2023. Following the introduction of the first biosimilar in July 2021, the consumption of the originator decreased by 0.5% per month (P = 0.008), and the growth rate of overall trastuzumab consumption decreased by 1.1% per month (P = 0.014). LCTM fit the best when the number of trajectory classes was two, dividing 30 PLADs into a group demonstrating a faster increase in biosimilar penetration and the other with a slower increase. The PLADs in the fast-increasing group had a higher proportion of the population covered by the national basic health insurance, a lower proportion of the urban population, a lower proportion of the population covered by the urban employee health insurance program, a lower gross domestic product per capita, a lower total health expenditure per capita, and a lower out-of-pocket expenditure. Conclusions The uptake of trastuzumab biosimilars in China was lower compared with major European countries. The introduction of trastuzumab biosimilars presented a substitutional effect. Perceptions of physicians and patients, the medicines procurement model, competition from other biologics, and health insurance payment methods may influence biosimilar uptake. Enhancing a comprehensive understanding of biosimilars among physicians and patients, including biologics with biosimilars in the national pooled procurement, and implementing provider payment reforms could foster biosimilar penetration.

Escalating the production and application of tungsten disulfide (WS2) nanosheets inevitably increases environmental human exposure and warrants the necessity of studies to elucidate their biological impacts. Herein, we assessed the toxicity of WS2 nanosheets and focused on the impacts of low doses (≤10 μg/mL) on normal (BEAS-2B) and tumorigenic (A549) lung epithelial cells. The low doses, which approximate real-world exposures, were found to induce cell apoptosis, while doses ≥ 50 μg/mL cause necrosis. Focused studies on low-dose exposure to WS2 nanosheets revealed more details of the impacts on both cell lines, including reduction of cell metabolic activity, induction of lipid peroxidation in cell membranes, and uncoupling of mitochondrial oxidative phosphorylation that led to the loss of ATP production. These phenomena, along with the expression situations of a few key proteins involved in apoptosis, point toward the occurrence of mitochondria-dependent apoptotic signaling in exposed cells. Substantial differences in responses to WS2 exposure between normal and tumorigenic lung epithelial cells were noticed as well. Specifically, BEAS-2B cells experienced more adverse effects and took up more nanosheets than A549 cells. Our results highlight the importance of dose and cell model selection in the assessment of nanotoxicity. By using doses consistent with real-world exposures and comparing normal and diseased cells, we can gain knowledge to guide the development of safety precautions for mitigating the adverse impacts of nanomaterial exposure on human health.

Background and Aim: Neonatal and maternal tetanus have remained endemic in tropical countries of Asia and Africa, with high fatality. Vaccination of women of childbearing age is the main strategy for their prevention/elimination. However, the uptake of these vaccines among these women has remained dismally low in these parts of the world. This study looked at the uptake and determinants of tetanus vaccination among the female clinical medical students of Bayero University, Kano, purposely looking at the interplay between knowledge and other factors on vaccine uptake. Materials and Methods: This was a cross-sectional study of female medical students aged 15–45 years, selected using probability proportionate to size and simple random sampling. The required information was collected using a validated semi-structured questionnaire. The data were analyzed using the Statistical Product and Service Solutions (SPSS) for Windows version 23. Prevalences of tetanus vaccination were calculated. The presence of a statistically significant difference in prevalence and other characteristics of the study groups were determined using appropriate tests. P 7). The prevalence of tetanus toxoid (TT)1 and TT2 was 20.7% and 17.1%, respectively. Being married, ever pregnant, and higher level of study were significantly associated with being vaccinated (χ2/P =59.093/P

Abstract Background Exercise Referral Schemes (ERSs) have been implemented across Western nations to stimulate an increase in adult physical activity but evidence of their effectiveness and cost-effectiveness is equivocal. Poor ERS uptake and adherence can have a negative impact on effectiveness and cost-effectiveness and, if patterned by socio-demographic factors, can also introduce or widen health inequalities. Different modes of ERS delivery have the potential to reduce costs and enhance uptake and adherence. The primary aim of this study was to examine the effect of different programmes of ERS delivery on scheme uptake and adherence. Secondary aims were to examine the effect of socio-demographic factors on scheme uptake and adherence, and the impact of delivery mode on the expected resource and corresponding costs of delivering core parts of the programme. Methods This was an observational cohort study with cost analysis. Routine monitoring data covering a three-year period (2019–2021) from one large UK ERS (number of patients = 28,917) were analysed. During this period three different programmes of delivery were operated in succession: standard (all sessions delivered face-to-face at a designated physical location), hybrid (sessions initially delivered face-to-face and then switched to remote delivery in response to the Covid-19 pandemic), and modified (sessions delivered face-to-face, remotely, or a combination of the two, as determined on a case-by-case basis according to Covid-19 risk and personal preferences). Multi-level binary logistic and linear regression were performed to examine the effect of programme of delivery and socio-demographic characteristics on uptake and adherence. Cost data were sourced from regional-level coordinators and through NERS audits supplied by national-level NERS managers and summarised using descriptive statistics. Results There was no effect of programme of delivery on scheme uptake. In comparison to those on the standard programme (who attended a mean of 23.1 exercise sessions) those on the modified programme had higher adherence (mean attendance of 25.7 sessions) while those on the hybrid programme had lower adherence (mean attendance of 19.4 sessions). Being older, or coming from an area of lower deprivation, increased the likelihood of uptake and adherence. Being female increased the chance of uptake but was associated with lower adherence. Patients referred to the programme from secondary care were more likely to take up the programme than those referred from primary care for prevention purposes, however their attendance at exercise sessions was lower. The estimated cost per person for face-to-face delivery of a typical 16-week cycle of the scheme was £65.42. The same cycle of the scheme delivered virtually (outside of a pandemic context) was estimated to cost £201.71 per person. Conclusions This study contributes new evidence concerning the effect of programme of delivery on ERS uptake and adherence and strengthens existing evidence concerning the effect of socio-economic factors. The findings direct the attention of ERS providers towards specific patient sub-groups who, if inequalities are to be addressed, require additional intervention to support uptake and adherence. At a time when providers may be considering alternative programmes of delivery, these findings challenge expectations that implementing virtual delivery will necessarily lead to cost savings.

Cadmium accumulation in wheat as a daily food, even in low concentrations, is a serious threat to human health. Previous studies have reported conflicting results on the impact of calcium treatments on cadmium uptake and translocation in plants due to the complex soil conditions. Our hydroponic study offers clearer insights into how specific calcium treatment parameters influence cadmium uptake and translocation in wheat. The hydroponic medium was contaminated by cadmium (CdCl2) and the following treatments were applied: CaCO3, CaSO4, CaCl2, CaCO3 + CaSO4, CaCO3 + CaCl2, and CaSO4 + CaCl2. After harvesting, the wheat was analyzed for Cd2+ uptake characteristics including translocation factor, bioconcentration factor, and uptake. Furthermore, physiological growth parameters and plant nutrients were also determined. Applying CaCO3 significantly decreased wheat Cd2+ concentration by about three times in CaCO3 and two times in CaCO3 + CaSO4 and CaCO3 + CaCl2 treatments than in Cd-control. This study clearly elucidates that pH and CO32− were crucial in reducing Cd2+ concentration in wheat. SO42−, Cl−, and Ca2+ showed no effect on Cd2+ concentration. Ca2+ only reduced the translocation factor (TF) of Cd2+ in plants. CaCO3 also declined cadmium interference in the Mg2+, Mn2+, and Cu2+ uptake. Therefore, this study provides novel insight into the pure effects of calcium treatments on controlling cadmium contamination in plants, independent of soil effect.

Background: Vaccine- preventable-diseases are major contributors to child mortality in Africa. In Nigeria, apart from the routine childhood vaccines that are provided free by the government, there are additional lifesaving non-routine vaccines like Rotavirus, Pneumococcal Conjugate (PCV), Varicella, Cerebrospinal meningitis, and Measles, Mumps, Rubella (MMR) vaccines. Until 2015 when PCV was included in the routine childhood immunisation, these five vaccines were optional and parents paid to immunize their children with them. Objective: To assess the level of knowledge and utilization of nonroutine immunisations among mothers. Methodology: A descriptive, cross -sectional study conducted in three infant welfare clinics purposively selected in Ibadan North Local Government Area, Oyo State, Nigeria. Participants were 110 mothers of children aged 6-24 months. A structured questionnaire was used for data collection and analysis done using SPSS version 20.0. Descriptive statistics were computed and Chi-square test was used for investigating association between categorical variables at 0.05 level of significance. Results: The findings revealed that 62 (56.4%) of the 110 mothers were aware of non-routine immunisation of which 23 (20.9%) had good knowledge about it. Only 23 (20.9%) of their children were immunized with all the nonroutine vaccines. High income, higher level of education and good knowledge level about non-routine immunisation of mothers were associated with the utilization of all non-routine immunisation among their children (p

Abstract 3D spheroids of primary human hepatocytes (3D PHH) retain a differentiated phenotype with largely conserved metabolic function and proteomic fingerprint over weeks in culture. As a result, 3D PHH are gaining importance as a model for mechanistic liver homeostasis studies and in vitro to in vivo extrapolation (IVIVE) in drug discovery. However, the kinetics and regulation of drug transporters have not yet been assessed in 3D PHH. Here, we used organic cation transporter 1 (OCT1/SLC22A1) as a model to study both transport kinetics and the long-term regulation of transporter activity via relevant signalling pathways. The kinetics of the OCT1 transporter was studied using the fluorescent model substrate 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP+) and known OCT1 inhibitors in individual 3D PHH. For long-term studies, 3D PHH were treated with xenobiotics for seven days, after which protein expression and OCT1 function were assessed. Global proteomic analysis was used to track hepatic phenotypes as well as prototypical changes in other regulated proteins, such as P-glycoprotein and Cytochrome P450 3A4. ASP+ kinetics indicated a fully functional OCT1 transporter with a Km value of 14 ± 4.0µM as the mean from three donors. Co-incubation with known OCT1 inhibitors decreased the uptake of ASP+ in the 3D PHH spheroids by 35–52%. The long-term exposure studies showed that OCT1 is relatively stable upon activation of nuclear receptor signalling or exposure to compounds that could induce inflammation, steatosis or liver injury. Our results demonstrate that 3D PHH spheroids express physiologically relevant levels of fully active OCT1 and that its transporter kinetics can be accurately studied in the 3D PHH configuration. We also confirm that OCT1 remains stable and functional during the activation of key metabolic pathways that alter the expression and function of other drug transporters and drug-metabolizing enzymes. These results will expand the range of studies that can be performed using 3D PHH.

A two-year field experiment was conducted at Lovely Professional University, Phagwara during the Rabi season of 2018–2019 and 2019-20 to study the effect of crop establishment methods and sowing schedule on the growth and yield of wheat under the rice-wheat cropping system. The experiment comprised different rice residue management-based wheat establishment methods, sowing dates, and varying nitrogen levels. The results confirmed that growth, yield and yield attributes were all influenced by different crop establishment methods, sowing dates and level of nitrogen application. The results showed that maximum plant height, number of tillers per plant, number of spikes per plant, spike length, number of grains per spike and grain yield were observed under 20th November sowing (timely sowing) and with 100% RDN (recommended dose of nitrogen). Moreover, the maximum number of spikes per plant, spike length and grain yield were significantly highest for the residue incorporation method of wheat establishment. The optimum time of sowing also improved the grain yield of wheat. Among the different crop establishment methods, the best attainment of available nitrogen status in soil was found in residue incorporation treatment. [ABSTRACT FROM AUTHOR]

Lung cancer screening (LCS) has the potential to decrease mortality from lung cancer by 20%. Yet, more than a decade since LCS was established as an evidence-based practice, < 20% of the eligible population in the United States has been screened. This review focuses on critically appraising interventions that have been designed to increase the initial uptake of LCS, including how they address known barriers to LCS and their effectiveness in overcoming these barriers. Studies were categorized based on the primary barriers that they addressed: (1) identifying eligible patients (including enhancing awareness through smoking history collection, outreach, and education), (2) shared decision-making-related interventions, and (3) patient navigation interventions. Four of the studies included multicomponent interventions, which often included patient navigation as one of the components. Overall, the effectiveness of the studies reviewed at improving LCS uptake generally was modest and was limited by the multilevel barriers that need to be overcome. Multicomponent interventions generally were more effective at improving LCS uptake, but most studies still had relatively low completion of screening. Improving uptake of LCS requires learning from prior interventions to design multilevel interventions that address barriers to LCS at key steps and identifying which components of these interventions are effective and generalizable. [ABSTRACT FROM AUTHOR]

Background: Proper utilization of Insecticide Treated Nets (ITNs) has been shown to be very effective in preventing malaria and in reducing the incidence of the disease. Getting people to buy and utilize ITNs has proven very difficult especially in low-income settings of sub-Saharan Africa where the disease is endemic. This research work, therefore assesses the effectiveness of the application of social marketing strategies in encouraging the acquisition and utilization of ITNs. Focus of the Article: The marketing mix concept which is usually referred to as the 4Ps of social marketing-price, place, product and promotion-were put to test to identify how the respondents will respond to variation in any of them. The research aims to assess the people's response to a favorable change in the price of ITNs, product quality, product location and target advertisement. Research Question: The research questions that this study aimed to answer is; will the introduction of the traditional marketing mix of social marketing help drive the acquisition and utilization of ITNs by the people in rural communities of Imo State, Nigeria? Program Design/Approach: The research report contained in this manuscript is targeted at health products (tangible and intangible) marketing researchers in resource-limited settings. These researchers who may not be able to afford the acquisition of large quantity health products like the ITNs to conduct their study can adopt the method employed in this study. Here the "prospect" of using social marketing strategies like the traditional marketing mix, to determine a possible increase in the acquisition and utilization of ITNs by the people. Importance to the Social Marketing Field: This study describes how researchers in low-and-middle income countries could conduct a social marketing research on tangible products such as the ITN without really selling ITNs. The people's intent to buy and use ITNs were assessed. More than 10 previous studies from as far back as 1999 were assessed, none of them employed the current methodology. Methodology: This study was a descriptive cross-sectional study where a multi-stage sampling technique was employed to recruit the study participants. Results: It was found that 55.1% had ITNs, good utilization rate of 80.6% was recorded among those who possessed ITNs. Using the social marketing principle to assess respondents' intent to own and use ITNs, an increase of 27.2%, 38.1%, 31.3%, and 35.9% will be achieved if the cost of acquiring ITNs becomes affordable, when ITNs become accessible, when practical guidance on use was provided, and when quality was improved. The study also revealed that, the vigorous pursue of social marketing techniques can enhance the sales of ITNs and its usage among the people. Recommendation for Research or Practice: This study has justified the usefulness of social marketing in engendering positive behavioural change where people can be motivated to adopt a health innovation. It is therefore recommended that health innovations and products must be sold to the people using social marketing strategies as this has been proven not only to encourage adoption/acquisition, but also utilization. [ABSTRACT FROM AUTHOR]

Background and Aims: Despite a quick rollout of PrEP as a preventive method against Human Immunodeficiency Virus (HIV) infections in Zambia, adolescent and young adult populations have remained very vulnerable to HIV infection. This study assessed the awareness and willingness to use PrEP among University of Zambia (UNZA) students. Methods: Three hundred forty‐six students participated in this cross‐sectional study at UNZA between March and June 2021. A previously validated questionnaire assessed willingness to use PrEP. We tested the hypothesized pathways between sexual risk behavior and willingness to use PrEP using the structural equation model. Multivariate logistic regression analysis was employed to determine factors associated with willingness to use PrEP. Variables with a p‐value < 0.05 were considered statistically significant. Results: Of the 346 students, 271 (78.3%) were aware of PrEP, and 59 (17.1%) of the participants were willing to use PrEP. Only 17 (4.9%) of the participants had used PrEP before. In the multivariable logistic regression model, students who were aware of PrEP compared to those who were not (AOR = 3.03, 95% CI: 1.10, 8.40, p) were more likely to be willing to use PrEP. Sexual risk behavior indirectly and positively affected willingness to use PrEP through awareness of PrEP. Conclusion: Even though most students were aware of PrEP, the willingness to use this preventative measure is still low among UNZA students, resulting in low uptake. Therefore, concerted efforts are required to influence the willingness and uptake of PrEP, especially in high‐risk age groups such as university students. [ABSTRACT FROM AUTHOR]

The present study aimed to investigate effects of direct and indirect written corrective feedback (WCF) on the development of Korean EFL writing skills. Feedback was provided using the computer software Grammarly. Twenty-seven students of the English Department of Adult Life-long Education at a university in Seoul participated in this study. The researcher employed a three-stage design: (1) individual writing of a draft in class, (2) two types of feedback (direct WCF vs. indirect WCF, reformulation), and (3) individual rewriting in class. Participants' writing scores significantly improved after receiving feedback overall. In addition, errors in forms and clarity significantly reduced after the feedback, but not errors in word choices. Regarding feedback types, learning gains were more significant after direct WCF than reformulation, although errors in forms after direct WCF and word choice errors after reformulation significantly reduced among three types of errors. Additionally, most errors occurred in forms. The ratio of uptake was relatively low. The direct WCF group demonstrated a higher uptake in errors of forms and clarity, while word choice errors were more effectively addressed by reformulation. Theoretical and pedagogical implications of these findings are discussed. [ABSTRACT FROM AUTHOR]

Introduction: In South Africa over the past 20 years, immunisation has saved countless lives as well as prevented illnesses and disabilities. Despite this, vaccine-preventable illnesses remain a danger. The demand for and uptake of immunisation services are shaped by a variety of factors that can either act as barriers or facilitators to immunisation uptake. The aim of this project was to identify the supply and demand barriers and develop local strategies to improve childhood immunisation in four zero-dose districts in South Africa. Materials and Methods: This study used a mixed-method approach. In each of these four districts, 15 in-depth key informant interviews with health workers and local health managers and four focus group discussions (10 participants per focus group discussion) with community members and caregivers were held over a three-month period. Transcribed interviews were thematically analysed using qualitative analysis software (Nvivo®) into 10 factors as identified as important in influencing immunisation demand and uptake in previous studies. A further four were identified during the data analysis process. Results: Despite the varying role of factors affecting demand and uptake of immunisation services, three consistent findings stand out as major barriers across all districts. The first is interaction with healthcare staff. This clearly highlights the crucial role that the interactions between patients and staff play in shaping perceptions and behaviours related to immunisation services. The second is the overall experience of care at healthcare facilities. This emphasises the role that patient experience of services plays in perceptions and behaviours related to immunisation services. The third is family dynamics. This highlights the important role family dynamics play in shaping individuals' decisions regarding immunisation uptake as well as the impact it has on the ability of people to access health services. Discussion: The role played by the different factors in the demand and uptake of immunisation services varied across the four districts examined in this study. Each of the districts presents a unique landscape where different factors have varying degrees of importance in affecting the utilisation of immunisation services. In some districts, certain factors are major barriers, clearly hindering the demand and uptake of immunisation services, while in others, these same factors might be a relatively minor barrier. This discrepancy highlights the unique nature of healthcare challenges across the districts and the need for tailored strategy recommendations to address them effectively. [ABSTRACT FROM AUTHOR]

Malaria poses a significant threat to pregnant women in sub-Saharan Africa, necessitating effective interventions like the intermittent preventive treatment of malaria in pregnancy with sulfadoxine-pyrimethamine (IPTp-SP). However, challenges persist in the uptake and effectiveness of this intervention. This scoping review aims to explore IPTp-SP uptake in African countries, identify influencing factors, and assess its effectiveness in preventing malaria and adverse outcomes in pregnancy. This scoping review follows Arksey and O'Malley's framework, employing the PRISMA-ScR guidelines for reporting. Searches were conducted in PubMed, Embase, Scopus, JSTOR, Web of Science, Google Scholar, and ProQuest, focusing on studies post-2000 published in the English language. The search produced 15,153 records, of which 104 full-text records were eligible and 101 papers were included in this review. The findings suggest varying IPTp-SP uptake rates, spanning from 5.3% to 98.9%, with their effectiveness supported by longitudinal studies, randomised controlled-trials (RCTs), cross-sectional surveys, and mixed-method studies. IPTp-SP demonstrates efficacy in reducing malaria during pregnancy, placental parasitaemia, and anaemia episodes, alongside improved birth outcomes. Common adverse effects of IPTp-SP include prematurity and low birth weight. Facilitators of IPTp-SP uptake include education and ANC attendance, while commonly reported barriers included inadequate knowledge and healthcare system challenges. The findings also suggest adverse effects such as prematurity, low birth weight, and maternal and perinatal mortality associated with IPTp-SP uptake. It is vital to strengthen antenatal care services by integrating comprehensive counselling on IPTp-SP and address healthcare system challenges. Community engagement, women's empowerment, and context-specific interventions are necessary for promoting IPTp-SP uptake and improving maternal and neonatal health outcomes in Africa. [ABSTRACT FROM AUTHOR]

Cadmium accumulation in wheat as a daily food, even in low concentrations, is a serious threat to human health. Previous studies have reported conflicting results on the impact of calcium treatments on cadmium uptake and translocation in plants due to the complex soil conditions. Our hydroponic study offers clearer insights into how specific calcium treatment parameters influence cadmium uptake and translocation in wheat. The hydroponic medium was contaminated by cadmium (CdCl2) and the following treatments were applied: CaCO3, CaSO4, CaCl2, CaCO3 + CaSO4, CaCO3 + CaCl2, and CaSO4 + CaCl2. After harvesting, the wheat was analyzed for Cd2+ uptake characteristics including translocation factor, bioconcentration factor, and uptake. Furthermore, physiological growth parameters and plant nutrients were also determined. Applying CaCO3 significantly decreased wheat Cd2+ concentration by about three times in CaCO3 and two times in CaCO3 + CaSO4 and CaCO3 + CaCl2 treatments than in Cd-control. This study clearly elucidates that pH and CO32− were crucial in reducing Cd2+ concentration in wheat. SO42−, Cl−, and Ca2+ showed no effect on Cd2+ concentration. Ca2+ only reduced the translocation factor (TF) of Cd2+ in plants. CaCO3 also declined cadmium interference in the Mg2+, Mn2+, and Cu2+ uptake. Therefore, this study provides novel insight into the pure effects of calcium treatments on controlling cadmium contamination in plants, independent of soil effect. [ABSTRACT FROM AUTHOR]

Aconitate decarboxylase-1 (ACOD1) is expressed by activated macrophages and generates itaconate that exerts anti-microbial and immunoregulatory effects. ACOD1-itaconate is essential for macrophage-mediated control of the intracellular pathogen Coxiella (C.) burnetii, which causes Q fever. Two isomers of itaconate, mesaconate and citraconate, have overlapping yet distinct activity on macrophage metabolism and inflammatory gene expression. Here, we found that all three isomers inhibited the growth of C. burnetii in axenic culture in ACCM-2 medium. However, only itaconate reduced C. burnetii replication efficiently in Acod1-/- macrophages. In contrast, addition of citraconate strongly increased C. burnetii replication in Acod1+/- macrophages, whereas mesaconate weakly enhanced bacterial burden in Acod1-/- macrophages. Analysis of intracellular isomers showed that exogenous citraconate and mesaconate inhibited the generation of itaconate by infected Acod1+/- macrophages. Uptake of added isomers into Acod1-/- macrophages was increased after infection for itaconate and mesaconate, but not for citraconate. Mesaconate, but not citraconate, competed with itaconate for uptake into macrophages. Taken together, inhibition of itaconate generation by macrophages and interference with the uptake of extracellular itaconate could be identified as potential mechanisms behind the divergent effects of citraconate and mesaconate on C. burnetii replication in macrophages or in axenic culture. [ABSTRACT FROM AUTHOR]

Introduction: Dietary fatty acids play a pivotal role in energy metabolism and various physical functions. However, the molecular mechanisms underlying the absorption of long-chain fatty acids (LCFA) in ruminant animals remain poorly understood. To address this gap, we investigated the occurrence and distribution of genes and proteins previously characterized in nonruminant models within the gastrointestinal tract of cattle. Methods: Tissue samples from the rumen, descending duodenum, proximal jejunum, medial jejunum, distal jejunum, and distal ileum were collected at slaughter from eight Holstein steers. RT--PCR and capillary electrophoretic-based chemiluminescence were used to determine the mRNA and protein levels of genes and proteins involved in the uptake, activation, intracellular trafficking, re-esterification, and lipoprotein assembly of LCFA. Results and discussion: Measurable mRNA levels were found for 38 genes involved in the intestinal absorption of LCFA in all examined sections of the bovine gastrointestinal tract. The mRNA abundances of all genes were greater in the intestinal sections than in the rumen sections (p < 0.05). At the protein level, the expression of nine key transporters and enzymes, namely, CD36, SLC27A4, ACSL1, FABP1, FABP2, DGAT1, MTP, Apo-AI, and ACAT2, was detected in most of the intestinal sections. The distribution profile of these proteins in the small intestine suggested a prominent role for protein-mediated absorption of LCFA in the proximal and distal intestine in cattle. The low expression levels of most proteins in the rumen suggest little absorption of LCFA at this location in cattle. Overall, the findings from this study support the presence, in cattle, of genes and proteins involved in the intestinal absorption of LCFA described in models of nonruminants. Identifying the proteins involved in the absorption of LCFA in the small intestine is the first step in understanding how the expression of these proteins influences the capacity of the small intestine to absorb fatty acids in cattle. [ABSTRACT FROM AUTHOR]

As a novel succinate dehydrogenase inhibitor (SDHI), pydiflumetofen (PYD) exhibits broad-spectrum bactericidal activity in various crops; however, little is yet known about its absorption, translocation, and metabolic behavior within plants. Cucumber and tomato plants were cultured in hydroponic conditions spiked at 0.5 mg/L of PYD, and samples were collected at certain intervals to investigate the residual fate of PYD within the plants. The results demonstrated that PYD was readily absorbed by the roots of both plants, with mean root concentration factors (RCFs) of 5.6–12.3 for cucumber and 5.0–12.4 for tomato. PYD exhibited higher translocation ability from stems to leaves and limited from roots to stems in cucumber, while comparably weak root-to-stem and stem-to-leaf translocation were observed in tomato. By the end of the exposure period, a mass loss of 51.55% and 56.67% was observed, and six and three metabolites were found to be generated in the cucumber and tomato systems, respectively. This study provides a foundation for comprehending the uptake and translocation of PYD and offers novel insights into its potential risks to agricultural products and food safety. [ABSTRACT FROM AUTHOR]

The well-stirred model (WSM) is commonly used to predict the hepatic clearance in vivo (CL H) of drugs. The necessary intrinsic clearance of the unbound drug (CL int-in vitro-unbound) is generated in the in vitro assays in the presence of microsomes or hepatocytes but in the absence of plasma proteins. The value of CL int-in vitro-unbound can be extrapolated with the fraction unbound determined in vitro in plasma (fu p) only if the fraction unbound in vivo in liver is the same. However, this approach resulted to a systematic underprediction bias of CL H. With the goal of reducing this bias, two new models of fraction unbound were published in this journal. These models estimate the binding kinetics of the rates of association and dissociation of the drug-protein complex and propose that more dissociation in the liver compared to plasma will increase the fraction unbound available for the metabolism. Consequently, these two models generated higher values of fraction unbound, implying a lower underprediction bias of CL H with the WSM. The first model was developed by Poulin et al. and is referring to the value of fu p that is adjusted (fu -adjusted) to quantify the effect of a full dissociation of the drug-protein complex at the hepatocyte membrane in accordance with the theory of the albumin-facilitated hepatic uptake. A second model was developed by Yan et al. who presented a dynamic fraction unbound (fu -dynamic) measuring the real dissociation kinetics of the drug-protein complex with a new in vitro assay in the presence and absence of a recombinant liver enzyme in plasma. Therefore, the objective of this study was to make the first comparative assessment between these two models. The results indicate that, in general, the WSM combined with the values of fu -adjusted was the most accurate approach for predicting CL H. The WSM combined with the values of fu -dynamic has underperformed particularly with the acidic and neutral drugs binding to the albumin and presenting a low metabolic turnover in vitro. Therefore, the new in vitro assay for fu -dynamic gave an underprediction bias of CL H for these drug properties. However, the values of fu -adjusted are significantly higher than those values of fu -dynamic , and, this resulted to no underprediction bias, which is reinforcing the theory of the ALB-facilitated hepatic uptake. For the other neutral and acidic drugs, the models of fu -dynamic and fu -adjusted are in closer agreement. Finally, for the basic drugs, the models of fu -adjusted and fu -dynamic as well as a third model only considering a pH gradient effect on fu p are almost accurately equivalent. [ABSTRACT FROM AUTHOR]

The study aimed at examining the effect of Iron (Fe) management strategies on the redistribution of Fe fractions in soil and on the Fe bioavailability and phytic acid (PA) content in lentil grains. A pot experiment was conducted in Completely Randomized Design with 8 treatments replicated thrice. The treatments were Control (no fertilizer); Recommended dose of fertilizers (RDF) i.e. 20:40 (N: P2O5); RDF + Fe (basal application @10 kg/ha); RDF + Fe (foliar@ 1% (w/v) solution); RDF + Fe (basal + foliar); RDF + FeSB (biopriming with Fe solubilizing bacteria (Enterobacter cloacae strain BAU3) @ 5 mL/kg seed (1 x109 cfu mL−1)); RDF + Fe (seed nutripriming with 1% (w/v) solution); and RDF + (biopriming + nutripriming). Fe source for the treatments was Fe-EDDHA chelate. Higher Fe release from these fractions was observed under biopriming + nutripriming with water soluble Fe of 21.09 and 20.82 mg kg−1, respectively for the biopriming + nutripriming and sole biopriming treatments; and organically bound Fe of 230.95 and 211.36 mg kg−1 for the same treatments. These fractions contributed toward higher Fe uptake which was highest under biopriming + nutripriming. Values were statistically significant for biopriming and nutripriming and basal + foliar treatment. Lowest PA values in lentil grains as well as PA/Fe ratio were estimated under basal + foliar and biopriming + nutripriming treatments. Combined basal and foliar application of Fe esp. in chelated form and use of seed priming strategies have immense potential in enhancing the Fe bioavailability in grains from soil, seed or foliage. [ABSTRACT FROM AUTHOR]

Background: Bowel cancer screening using faecal immunochemical testing is provided in the United Kingdom with the aim of reducing mortality from colorectal cancer. However, there are low participant rates among ethnic minorities across the United Kingdom. Faith‐placed interventions have the potential to improve screening rates among such populations, but studies examining their effectiveness are scarce. Methods: We delivered a presentation on bowel cancer screening to 204 Muslims in seven mosques in East London (intervention group). All participants completed a questionnaire regarding attitudes, perceptions and knowledge of bowel cancer screening before and after the presentation. Concurrently, we administered the questionnaire to 72 subjects attending a mosque that did not receive the presentation (comparison group). Results: The intervention group showed a greater willingness to do the test (90% vs. 67%, p < 0.001) and to recommend it to others (96% vs. 74%, p < 0.001), ability to complete the test by themselves (94% vs. 56%, p < 0.001) and confidence in noticing symptoms (78% vs. 32%, p < 0.001) after the presentation compared to before. There was a significant difference between the intervention group post‐presentation and the comparison group on intention to do the test (90% vs. 79%, p = 0.02), recommending it to others (96% vs. 83%, p < 0.001), and confidence in their ability to complete the test by themselves (94% vs. 63%, p < 0.001). Conclusion: A culture‐sensitive, faith‐placed health education intervention delivered in mosques can substantially improve knowledge of bowel cancer screening and increase the intention to participate in the screening programme. Patient or Public Contribution: The intervention presentation was developed using insights from four public involvement sessions with four to six members representative of the East London Muslim community. The sessions sought attendees' thoughts on appropriate ways to approach the intervention design for their community and asked for their views on the acceptability, appropriateness of messaging, format/design and likely impact of the presentation. Their views were then utilised to improve the presentation. [ABSTRACT FROM AUTHOR]