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1. In vivo evolution of butane oxidation by terminal alkane hydroxylases AlkB and CYP153A6.

2. Production of renewable polymers from crop plants.

4. Establishment of new crops for the production of natural rubber.

5. Guayule and Russian dandelion as alternative sources of natural rubber.

6. Alkane hydroxylases involved in microbial alkane degradation.

7. Profiling mechanisms of alkane hydroxylase activity in vivo using the diagnostic substrate norcarane.

8. Prospects for biopolymer production in plants.

9. The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells.

10. CYP153A6, a soluble P450 oxygenase catalyzing terminal-alkane hydroxylation.

11. Selection of biocatalysts for chemical synthesis.

12. Cytochrome P450 alkane hydroxylases of the CYP153 family are common in alkane-degrading eubacteria lacking integral membrane alkane hydroxylases.

13. Poly(3-hydroxyalkanoate) polymerase synthesis and in vitro activity in recombinant Escherichia coli and Pseudomonas putida.

14. Expanding the alkane oxygenase toolbox: new enzymes and applications.

15. Biocatalytic production of perillyl alcohol from limonene by using a novel Mycobacterium sp. cytochrome P450 alkane hydroxylase expressed in Pseudomonas putida.

16. Expression of PHA polymerase genes of Pseudomonas putida in Escherichia coli and its effect on PHA formation.

17. Identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases.

18. Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2.

19. Characterization of two alkane hydroxylase genes from the marine hydrocarbonoclastic bacterium Alcanivorax borkumensis.

20. Biotransformation of limonene by bacteria, fungi, yeasts, and plants.

21. Practical issues in the application of oxygenases.

22. Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and Rhodococcus using polymerase chain reaction with highly degenerate primers.

23. Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa.

24. Gene cloning and characterization of multiple alkane hydroxylase systems in Rhodococcus strains Q15 and NRRL B-16531.

25. Alkane hydroxylase homologues in Gram-positive strains.

26. Enzyme technology: an overview.

27. Practical syntheses of N-substituted 3-hydroxyazetidines and 4-hydroxypiperidines by hydroxylation with Sphingomonas sp. HXN-200.

28. Oxidative biotransformations using oxygenases.

29. Functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria.

30. Rubredoxins involved in alkane oxidation.

31. Preparation of (R)- and (S)-N-protected 3-hydroxypyrrolidines by hydroxylation with Sphingomonas sp. HXN-200, a highly active, regio- and stereoselective, and easy to handle biocatalyst.

32. Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis.

33. New alkane-responsive expression vectors for Escherichia coli and pseudomonas.

34. The alkane hydroxylase gene of Burkholderia cepacia RR10 is under catabolite repression control.

35. Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes.

36. Detection of genes for alkane and naphthalene catabolism in Rhodococcus sp. strain 1BN.

37. Expression, stability and performance of the three-component alkane mono-oxygenase of Pseudomonas oleovorans in Escherichia coli.

38. Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains.

39. Determinants for overproduction of the Pseudomonas oleovorans cytoplasmic membrane protein alkane hydroxylase in alk+ Escherichia coli W3110.

40. Genetics of alkane oxidation by Pseudomonas oleovorans.

41. DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans.

42. Topology of the membrane-bound alkane hydroxylase of Pseudomonas oleovorans.

43. Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase.

44. Bioconversions of aliphatic compounds by Pseudomonas oleovorans in multiphase bioreactors: background and economic potential.

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