141 results on '"van der Lee, Theo A. J."'
Search Results
2. Adaptation of Fusarium Head Blight Pathogens to Changes in Agricultural Practices and Human Migration.
- Author
-
Yang, Meixin, Smit, Sandra, de Ridder, Dick, Feng, Jie, Liu, Taiguo, Xu, Jinrong, van der Lee, Theo A. J., Zhang, Hao, and Chen, Wanquan
- Subjects
HUMAN migrations ,CROPPING systems ,AGRICULTURE ,GENOMICS ,CROPS - Abstract
Fusarium head blight (FHB) is one of the most destructive wheat diseases worldwide. To understand the impact of human migration and changes in agricultural practices on crop pathogens, here population genomic analysis with 245 representative strains from a collection of 4,427 field isolates of Fusarium asiaticum, the causal agent of FHB in Southern China is conducted. Three populations with distinct evolution trajectories are identifies over the last 10,000 years that can be correlated with historically documented changes in agricultural practices due to human migration caused by the Southern Expeditions during the Jin Dynasty. The gradual decrease of 3ADON‐producing isolates from north to south along with the population structure and spore dispersal patterns shows the long‐distance (>250 km) dispersal of F. asiaticum. These insights into population dynamics and evolutionary history of FHB pathogens are corroborated by a genome‐wide analysis with strains originating from Japan, South America, and the USA, confirming the adaptation of FHB pathogens to cropping systems and human migration. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. The Pectobacterium pangenome, with a focus on Pectobacterium brasiliense, shows a robust core and extensive exchange of genes from a shared gene pool
- Author
-
Jonkheer, Eef M., Brankovics, Balázs, Houwers, Ilse M., van der Wolf, Jan M., Bonants, Peter J. M., Vreeburg, Robert A. M., Bollema, Robert, de Haan, Jorn R., Berke, Lidija, Smit, Sandra, de Ridder, Dick, and van der Lee, Theo A. J.
- Published
- 2021
- Full Text
- View/download PDF
4. A target enrichment approach for enhanced recovery of Synchytrium endobioticum nuclear genome sequences.
- Author
-
Nguyen, Hai D. T., Ponomareva, Ekaterina, Dadej, Kasia, Smith, Donna, Antoun, Melissa, van der Lee, Theo A. J., and van de Vossenberg, Bart T. L. H.
- Subjects
POTATOES ,GENE targeting ,GENOMES ,SEQUENCE analysis ,NUCLEOTIDE sequencing ,WARTS ,SPORES ,TUBERS - Abstract
Potato wart disease is caused by the obligate fungal pathogen Synchytrium endobioticum. DNA extraction from compost, purified spores and crude wart tissue derived from tuber galls of infected potatoes often results in low S. endobioticum DNA concentration or highly contaminated with DNA coming from other microorganisms and the potato host. Therefore, Illumina sequencing of these samples generally results in suboptimal recovery of the nuclear genome sequences of S. endobioticum. A hybridization-based target enrichment protocol was developed to strongly enhance the recovery of S. endobioticum DNA while off-target organisms DNA remains uncaptured. The design strategy involved creating a set of 180,000 molecular baits targeting both gene and non-gene regions of S. endobioticum. The baits were applied to whole genome amplified DNA samples of various S. endobioticum pathotypes (races) in compost, from purified spores and crude wart tissue samples. This was followed by Illumina sequencing and bioinformatic analyses. Compared to non-enriched samples, target enriched samples: 1) showed a significant increase in the proportion of sequenced bases mapped to the S. endobioticum nuclear genome, especially for crude wart tissue samples; 2) yielded sequencing data with higher and better nuclear genome coverage; 3) biased genome assembly towards S. endobioticum sequences, yielding smaller assembly sizes but higher representation of putative S. endobioticum contigs; 4) showed an increase in the number of S. endobioticum genes detected in the genome assemblies. Our hybridization-based target enrichment protocol offers a valuable tool for enhancing genome sequencing and NGS-based molecular detection of S. endobioticum, especially in difficult samples. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
5. Dead or Alive, that is the question: development and assessment of molecular Synchytrium endobioticum viability tests
- Author
-
van de Vossenberg, Bart T. L. H., primary, Smith, Donna, additional, van Gent-Pelzer, Marga P. E., additional, van den Berg, Marlies, additional, Govaert, Marcel, additional, Helderman, Carin M., additional, and van der Lee, Theo A. J., additional
- Published
- 2023
- Full Text
- View/download PDF
6. An Efficient Triplex TaqMan Quantitative PCR to Detect a Blackleg-Causing Lineage of Pectobacterium brasiliense in Potato Based on a Pangenome Analysis
- Author
-
van der Lee, Theo A. J., primary, van Gent-Pelzer, Marga P. E., additional, Jonkheer, Eef M., additional, Brankovics, Balázs, additional, Houwers, Ilse M., additional, van der Wolf, Jan M., additional, Bonants, Peter J. M., additional, van Duivenbode, Inge, additional, Vreeburg, Robert A. M., additional, Nas, Mathijs, additional, and Smit, Sandra, additional
- Published
- 2023
- Full Text
- View/download PDF
7. Stress and sexual reproduction affect the dynamics of the wheat pathogen effector AvrStb6 and strobilurin resistance
- Author
-
Kema, Gerrit H. J., Mirzadi Gohari, Amir, Aouini, Lamia, Gibriel, Hesham A. Y., Ware, Sarah B., van den Bosch, Frank, Manning-Smith, Robbie, Alonso-Chavez, Vasthi, Helps, Joe, Ben M’Barek, Sarrah, Mehrabi, Rahim, Diaz-Trujillo, Caucasella, Zamani, Elham, Schouten, Henk J., van der Lee, Theo A. J., Waalwijk, Cees, de Waard, Maarten A., de Wit, Pierre J. G. M., Verstappen, Els C. P., Thomma, Bart P. H. J., Meijer, Harold J. G., and Seidl, Michael F.
- Published
- 2018
- Full Text
- View/download PDF
8. Comparative genomics of chytrid fungi reveal insights into the obligate biotrophic and pathogenic lifestyle of Synchytrium endobioticum
- Author
-
van de Vossenberg, Bart T. L. H., Warris, Sven, Nguyen, Hai D. T., van Gent-Pelzer, Marga P. E., Joly, David L., van de Geest, Henri C., Bonants, Peter J. M., Smith, Donna S., Lévesque, C. André, and van der Lee, Theo A. J.
- Published
- 2019
- Full Text
- View/download PDF
9. A real-time TaqMan PCR assay to discriminate between pathotype 1 (D1) and non-pathotype 1 (D1) isolates of Synchytrium endobioticum
- Author
-
Bonants, Peter J. M., van Gent-Pelzer, Marga P. E., van Leeuwen, Gerard C. M., and van der Lee, Theo A. J.
- Published
- 2015
- Full Text
- View/download PDF
10. PanTools v3: functional annotation, classification and phylogenomics
- Author
-
Jonkheer, Eef M, primary, van Workum, Dirk-Jan M, additional, Sheikhizadeh Anari, Siavash, additional, Brankovics, Balázs, additional, de Haan, Jorn R, additional, Berke, Lidija, additional, van der Lee, Theo A J, additional, de Ridder, Dick, additional, and Smit, Sandra, additional
- Published
- 2022
- Full Text
- View/download PDF
11. Diagnosis of Fusarium Infections: Approaches to Identification by the Clinical Mycology Laboratory
- Author
-
van Diepeningen, Anne D., Brankovics, Balázs, Iltes, Jearidienne, van der Lee, Theo A. J., and Waalwijk, Cees
- Published
- 2015
- Full Text
- View/download PDF
12. Live and dead qPCR detection demonstrates that feeding of Nosema ceranae results in infection in the honey bee but not the bumble bee
- Author
-
van der Steen, Jozef J. M., primary, Hendriks, Marc J. A., additional, van Diepeningen, Anne D., additional, van Gent-Pelzer, Marga P. E., additional, and van der Lee, Theo A. J., additional
- Published
- 2022
- Full Text
- View/download PDF
13. Correction: A target enrichment approach for enhanced recovery of Synchytrium endobioticum nuclear genome sequences.
- Author
-
Nguyen, Hai D. T., Ponomareva, Ekaterina, Dadej, Kasia, Smith, Donna, Antoun, Melissa, van der Lee, Theo A. J., and van de Vossenberg, Bart T. L. H.
- Subjects
COPYRIGHT licenses - Published
- 2024
- Full Text
- View/download PDF
14. Phylogenomic Analysis of a 55.1-kb 19-Gene Dataset Resolves a Monophyletic Fusarium that Includes the Fusarium solani Species Complex
- Author
-
Geiser, David M., primary, Al-Hatmi, Abdullah M. S., additional, Aoki, Takayuki, additional, Arie, Tsutomu, additional, Balmas, Virgilio, additional, Barnes, Irene, additional, Bergstrom, Gary C., additional, Bhattacharyya, Madan K., additional, Blomquist, Cheryl L., additional, Bowden, Robert L., additional, Brankovics, Balázs, additional, Brown, Daren W., additional, Burgess, Lester W., additional, Bushley, Kathryn, additional, Busman, Mark, additional, Cano-Lira, José F., additional, Carrillo, Joseph D., additional, Chang, Hao-Xun, additional, Chen, Chi-Yu, additional, Chen, Wanquan, additional, Chilvers, Martin, additional, Chulze, Sofia, additional, Coleman, Jeffrey J., additional, Cuomo, Christina A., additional, de Beer, Z. Wilhelm, additional, de Hoog, G. Sybren, additional, Del Castillo-Múnera, Johanna, additional, Del Ponte, Emerson M., additional, Diéguez-Uribeondo, Javier, additional, Di Pietro, Antonio, additional, Edel-Hermann, Véronique, additional, Elmer, Wade H., additional, Epstein, Lynn, additional, Eskalen, Akif, additional, Esposto, Maria Carmela, additional, Everts, Kathryne L., additional, Fernández-Pavía, Sylvia P., additional, da Silva, Gilvan Ferreira, additional, Foroud, Nora A., additional, Fourie, Gerda, additional, Frandsen, Rasmus J. N., additional, Freeman, Stanley, additional, Freitag, Michael, additional, Frenkel, Omer, additional, Fuller, Kevin K., additional, Gagkaeva, Tatiana, additional, Gardiner, Donald M., additional, Glenn, Anthony E., additional, Gold, Scott E., additional, Gordon, Thomas R., additional, Gregory, Nancy F., additional, Gryzenhout, Marieka, additional, Guarro, Josep, additional, Gugino, Beth K., additional, Gutierrez, Santiago, additional, Hammond-Kosack, Kim E., additional, Harris, Linda J., additional, Homa, Mónika, additional, Hong, Cheng-Fang, additional, Hornok, László, additional, Huang, Jenn-Wen, additional, Ilkit, Macit, additional, Jacobs, Adriaana, additional, Jacobs, Karin, additional, Jiang, Cong, additional, Jiménez-Gasco, María del Mar, additional, Kang, Seogchan, additional, Kasson, Matthew T., additional, Kazan, Kemal, additional, Kennell, John C., additional, Kim, Hye-Seon, additional, Kistler, H. Corby, additional, Kuldau, Gretchen A., additional, Kulik, Tomasz, additional, Kurzai, Oliver, additional, Laraba, Imane, additional, Laurence, Matthew H., additional, Lee, Theresa, additional, Lee, Yin-Won, additional, Lee, Yong-Hwan, additional, Leslie, John F., additional, Liew, Edward C. Y., additional, Lofton, Lily W., additional, Logrieco, Antonio F., additional, S. López-Berges, Manuel, additional, Luque, Alicia G., additional, Lysøe, Erik, additional, Ma, Li-Jun, additional, Marra, Robert E., additional, Martin, Frank N., additional, May, Sara R., additional, McCormick, Susan P., additional, McGee, Chyanna, additional, Meis, Jacques F., additional, Migheli, Quirico, additional, Mohamed Nor, N. M. I., additional, Monod, Michel, additional, Moretti, Antonio, additional, Mostert, Diane, additional, Mulè, Giuseppina, additional, Munaut, Françoise, additional, Munkvold, Gary P., additional, Nicholson, Paul, additional, Nucci, Marcio, additional, O’Donnell, Kerry, additional, Pasquali, Matias, additional, Pfenning, Ludwig H., additional, Prigitano, Anna, additional, Proctor, Robert H., additional, Ranque, Stéphane, additional, Rehner, Stephen A., additional, Rep, Martijn, additional, Rodríguez-Alvarado, Gerardo, additional, Rose, Lindy Joy, additional, Roth, Mitchell G., additional, Ruiz-Roldán, Carmen, additional, Saleh, Amgad A., additional, Salleh, Baharuddin, additional, Sang, Hyunkyu, additional, Scandiani, María Mercedes, additional, Scauflaire, Jonathan, additional, Schmale, David G., additional, Short, Dylan P. G., additional, Šišić, Adnan, additional, Smith, Jason A., additional, Smyth, Christopher W., additional, Son, Hokyoung, additional, Spahr, Ellie, additional, Stajich, Jason E., additional, Steenkamp, Emma, additional, Steinberg, Christian, additional, Subramaniam, Rajagopal, additional, Suga, Haruhisa, additional, Summerell, Brett A., additional, Susca, Antonella, additional, Swett, Cassandra L., additional, Toomajian, Christopher, additional, Torres-Cruz, Terry J., additional, Tortorano, Anna M., additional, Urban, Martin, additional, Vaillancourt, Lisa J., additional, Vallad, Gary E., additional, van der Lee, Theo A. J., additional, Vanderpool, Dan, additional, van Diepeningen, Anne D., additional, Vaughan, Martha M., additional, Venter, Eduard, additional, Vermeulen, Marcele, additional, Verweij, Paul E., additional, Viljoen, Altus, additional, Waalwijk, Cees, additional, Wallace, Emma C., additional, Walther, Grit, additional, Wang, Jie, additional, Ward, Todd J., additional, Wickes, Brian L., additional, Wiederhold, Nathan P., additional, Wingfield, Michael J., additional, Wood, Ana K. M., additional, Xu, Jin-Rong, additional, Yang, Xiao-Bing, additional, Yli-Mattila, Tapani, additional, Yun, Sung-Hwan, additional, Zakaria, Latiffah, additional, Zhang, Hao, additional, Zhang, Ning, additional, Zhang, Sean X., additional, and Zhang, Xue, additional
- Published
- 2021
- Full Text
- View/download PDF
15. Additional file 2 of The Pectobacterium pangenome, with a focus on Pectobacterium brasiliense, shows a robust core and extensive exchange of genes from a shared gene pool
- Author
-
Jonkheer, Eef M., Brankovics, Balázs, Houwers, Ilse M., Van Der Wolf, Jan M., Bonants, Peter J. M., Vreeburg, Robert A. M., Bollema, Robert, De Haan, Jorn R., Berke, Lidija, Smit, Sandra, De Ridder, Dick, and Van Der Lee, Theo A. J.
- Abstract
Additional file 2: Figure S1. Number of core, accessory and unique genes per genome. Figure S2. Effect of loosening core and unique threshold. Figure S3. Size of an 87 P. brasiliense pangenome. Figure S4. Size of a pangenome of 110 strains in 19 different Pectobacterium species. Figure S5. Size of a 27 P. brasiliense strain pangenome. Figure S6. Core SNP tree with only a single representative genome per Pectobacterium and Dickeya species. Figure S7. Presence of pectinase genes in 197 Pectobacterium genomes. Figure S8. Core SNP tree. Figure S9. Multilocus sequence analysis (MLSA) tree. Figure S10. Average Nucleotide Identity (ANI) tree. Figure S11. K-mer distance tree. Figure S12. Gene distance tree.
- Published
- 2021
- Full Text
- View/download PDF
16. Additional file 3 of The Pectobacterium pangenome, with a focus on Pectobacterium brasiliense, shows a robust core and extensive exchange of genes from a shared gene pool
- Author
-
Jonkheer, Eef M., Brankovics, Balázs, Houwers, Ilse M., Van Der Wolf, Jan M., Bonants, Peter J. M., Vreeburg, Robert A. M., Bollema, Robert, De Haan, Jorn R., Berke, Lidija, Smit, Sandra, De Ridder, Dick, and Van Der Lee, Theo A. J.
- Abstract
Additional file 3. Supplementary analyses. Functional annotation and enrichment analysis.
- Published
- 2021
- Full Text
- View/download PDF
17. Phylogenomic Analysis of a 55.1-kb 19-Gene Dataset Resolves a Monophyletic Fusarium that Includes the Fusarium solani Species Complex
- Author
-
Geiser, David M, Al-Hatmi, Abdullah M S, Aoki, Takayuki, Arie, Tsutomu, Balmas, Virgilio, Barnes, Irene, Bergstrom, Gary C, Bhattacharyya, Madan K, Blomquist, Cheryl L, Bowden, Robert L, Brankovics, Balázs, Brown, Daren W, Burgess, Lester W, Bushley, Kathryn, Busman, Mark, Cano-Lira, José F, Carrillo, Joseph D, Chang, Hao-Xun, Chen, Chi-Yu, Chen, Wanquan, Chilvers, Martin, Chulze, Sofia, Coleman, Jeffrey J, Cuomo, Christina A, de Beer, Z Wilhelm, de Hoog, G Sybren, Del Castillo-Múnera, Johanna, Del Ponte, Emerson M, Diéguez-Uribeondo, Javier, Di Pietro, Antonio, Edel-Hermann, Véronique, Elmer, Wade H, Epstein, Lynn, Eskalen, Akif, Esposto, Maria Carmela, Everts, Kathryne L, Fernández-Pavía, Sylvia P, da Silva, Gilvan Ferreira, Foroud, Nora A, Fourie, Gerda, Frandsen, Rasmus J. N., Freeman, Stanley, Freitag, Michael, Frenkel, Omer, Fuller, Kevin K, Gagkaeva, Tatiana, Gardiner, Donald M, Glenn, Anthony E, Gold, Scott E, Gordon, Thomas R, Gregory, Nancy F, Gryzenhout, Marieka, Guarro, Josep, Gugino, Beth K, Gutierrez, Santiago, Hammond-Kosack, Kim E, Harris, Linda J, Homa, Mónika, Hong, Cheng-Fang, Hornok, László, Huang, Jenn-Wen, Ilkit, Macit, Jacobs, Adriaana, Jacobs, Karin, Jiang, Cong, Jiménez-Gasco, María Del Mar, Kang, Seogchan, Kasson, Matthew T, Kazan, Kemal, Kennell, John C, Kim, Hye-Seon, Kistler, H Corby, Kuldau, Gretchen A, Kulik, Tomasz, Kurzai, Oliver, Laraba, Imane, Laurence, Matthew H, Lee, Theresa, Lee, Yin-Won, Lee, Yong-Hwan, Leslie, John F, Liew, Edward C Y, Lofton, Lily W, Logrieco, Antonio F, S López-Berges, Manuel, Luque, Alicia G, Lysøe, Erik, Ma, Li-Jun, Marra, Robert E, Martin, Frank N, May, Sara R, McCormick, Susan P, McGee, Chyanna, Meis, Jacques F, Migheli, Quirico, Mohamed Nor, N M I, Monod, Michel, Moretti, Antonio, Mostert, Diane, Mulè, Giuseppina, Munaut, Françoise, Munkvold, Gary P, Nicholson, Paul, Nucci, Marcio, O'Donnell, Kerry, Pasquali, Matias, Pfenning, Ludwig H, Prigitano, Anna, Proctor, Robert H, Ranque, Stéphane, Rehner, Stephen A, Rep, Martijn, Rodríguez-Alvarado, Gerardo, Rose, Lindy Joy, Roth, Mitchell G, Ruiz-Roldán, Carmen, Saleh, Amgad A, Salleh, Baharuddin, Sang, Hyunkyu, Scandiani, María Mercedes, Scauflaire, Jonathan, Schmale, David G, Short, Dylan P G, Šišić, Adnan, Smith, Jason A, Smyth, Christopher W, Son, Hokyoung, Spahr, Ellie, Stajich, Jason E, Steenkamp, Emma, Steinberg, Christian, Subramaniam, Rajagopal, Suga, Haruhisa, Summerell, Brett A, Susca, Antonella, Swett, Cassandra L, Toomajian, Christopher, Torres-Cruz, Terry J, Tortorano, Anna M, Urban, Martin, Vaillancourt, Lisa J, Vallad, Gary E, van der Lee, Theo A J, Vanderpool, Dan, van Diepeningen, Anne D, Vaughan, Martha M, Venter, Eduard, Vermeulen, Marcele, Verweij, Paul E, Viljoen, Altus, Waalwijk, Cees, Wallace, Emma C, Walther, Grit, Wang, Jie, Ward, Todd J, Wickes, Brian L, Wiederhold, Nathan P, Wingfield, Michael J, Wood, Ana K M, Xu, Jin-Rong, Yang, Xiao-Bing, Yli-Mattila, Tapani, Yun, Sung-Hwan, Zakaria, Latiffah, Zhang, Hao, Zhang, Ning, Zhang, Sean X, Zhang, Xue, Geiser, David M, Al-Hatmi, Abdullah M S, Aoki, Takayuki, Arie, Tsutomu, Balmas, Virgilio, Barnes, Irene, Bergstrom, Gary C, Bhattacharyya, Madan K, Blomquist, Cheryl L, Bowden, Robert L, Brankovics, Balázs, Brown, Daren W, Burgess, Lester W, Bushley, Kathryn, Busman, Mark, Cano-Lira, José F, Carrillo, Joseph D, Chang, Hao-Xun, Chen, Chi-Yu, Chen, Wanquan, Chilvers, Martin, Chulze, Sofia, Coleman, Jeffrey J, Cuomo, Christina A, de Beer, Z Wilhelm, de Hoog, G Sybren, Del Castillo-Múnera, Johanna, Del Ponte, Emerson M, Diéguez-Uribeondo, Javier, Di Pietro, Antonio, Edel-Hermann, Véronique, Elmer, Wade H, Epstein, Lynn, Eskalen, Akif, Esposto, Maria Carmela, Everts, Kathryne L, Fernández-Pavía, Sylvia P, da Silva, Gilvan Ferreira, Foroud, Nora A, Fourie, Gerda, Frandsen, Rasmus J. N., Freeman, Stanley, Freitag, Michael, Frenkel, Omer, Fuller, Kevin K, Gagkaeva, Tatiana, Gardiner, Donald M, Glenn, Anthony E, Gold, Scott E, Gordon, Thomas R, Gregory, Nancy F, Gryzenhout, Marieka, Guarro, Josep, Gugino, Beth K, Gutierrez, Santiago, Hammond-Kosack, Kim E, Harris, Linda J, Homa, Mónika, Hong, Cheng-Fang, Hornok, László, Huang, Jenn-Wen, Ilkit, Macit, Jacobs, Adriaana, Jacobs, Karin, Jiang, Cong, Jiménez-Gasco, María Del Mar, Kang, Seogchan, Kasson, Matthew T, Kazan, Kemal, Kennell, John C, Kim, Hye-Seon, Kistler, H Corby, Kuldau, Gretchen A, Kulik, Tomasz, Kurzai, Oliver, Laraba, Imane, Laurence, Matthew H, Lee, Theresa, Lee, Yin-Won, Lee, Yong-Hwan, Leslie, John F, Liew, Edward C Y, Lofton, Lily W, Logrieco, Antonio F, S López-Berges, Manuel, Luque, Alicia G, Lysøe, Erik, Ma, Li-Jun, Marra, Robert E, Martin, Frank N, May, Sara R, McCormick, Susan P, McGee, Chyanna, Meis, Jacques F, Migheli, Quirico, Mohamed Nor, N M I, Monod, Michel, Moretti, Antonio, Mostert, Diane, Mulè, Giuseppina, Munaut, Françoise, Munkvold, Gary P, Nicholson, Paul, Nucci, Marcio, O'Donnell, Kerry, Pasquali, Matias, Pfenning, Ludwig H, Prigitano, Anna, Proctor, Robert H, Ranque, Stéphane, Rehner, Stephen A, Rep, Martijn, Rodríguez-Alvarado, Gerardo, Rose, Lindy Joy, Roth, Mitchell G, Ruiz-Roldán, Carmen, Saleh, Amgad A, Salleh, Baharuddin, Sang, Hyunkyu, Scandiani, María Mercedes, Scauflaire, Jonathan, Schmale, David G, Short, Dylan P G, Šišić, Adnan, Smith, Jason A, Smyth, Christopher W, Son, Hokyoung, Spahr, Ellie, Stajich, Jason E, Steenkamp, Emma, Steinberg, Christian, Subramaniam, Rajagopal, Suga, Haruhisa, Summerell, Brett A, Susca, Antonella, Swett, Cassandra L, Toomajian, Christopher, Torres-Cruz, Terry J, Tortorano, Anna M, Urban, Martin, Vaillancourt, Lisa J, Vallad, Gary E, van der Lee, Theo A J, Vanderpool, Dan, van Diepeningen, Anne D, Vaughan, Martha M, Venter, Eduard, Vermeulen, Marcele, Verweij, Paul E, Viljoen, Altus, Waalwijk, Cees, Wallace, Emma C, Walther, Grit, Wang, Jie, Ward, Todd J, Wickes, Brian L, Wiederhold, Nathan P, Wingfield, Michael J, Wood, Ana K M, Xu, Jin-Rong, Yang, Xiao-Bing, Yli-Mattila, Tapani, Yun, Sung-Hwan, Zakaria, Latiffah, Zhang, Hao, Zhang, Ning, Zhang, Sean X, and Zhang, Xue
- Abstract
Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. In 2013, the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agriculturally and clinically important Fusarium species, including the F. solani species complex (FSSC). Subsequently, this concept was challenged in 2015 by one research group who proposed dividing the genus Fusarium into seven genera, including the FSSC described as members of the genus Neocosmospora, with subsequent justification in 2018 based on claims that the 2013 concept of Fusarium is polyphyletic. Here, we test this claim and provide a phylogeny based on exonic nucleotide sequences of 19 orthologous protein-coding genes that strongly support the monophyly of Fusarium including the FSSC. We reassert the practical and scientific argument in support of a genus Fusarium that includes the FSSC and several other basal lineages, consistent with the longstanding use of this name among plant pathologists, medical mycologists, quarantine officials, regulatory agencies, students, and researchers with a stake in its taxonomy. In recognition of this monophyly, 40 species described as genus Neocosmospora were recombined in genus Fusarium, and nine others were renamed Fusarium. Here the global Fusarium community voices strong support for the inclusion of the FSSC in Fusarium, as it remains the best scientific, nomenclatural, and practical taxonomic option available.
- Published
- 2021
18. New broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat
- Author
-
Tabib Ghaffary, S. Mahmod, Faris, Justin D., Friesen, Timothy L., Visser, Richard G. F., van der Lee, Theo A. J., Robert, Olivier, and Kema, Gert H. J.
- Published
- 2012
- Full Text
- View/download PDF
19. Genetic analysis of resistance to septoria tritici blotch in the French winter wheat cultivars Balance and Apache
- Author
-
Tabib Ghaffary, Seyed Mahmod, Robert, Olivier, Laurent, Valerie, Lonnet, Philippe, Margalé, Eric, van der Lee, Theo A. J., Visser, Richard G. F., and Kema, Gert H. J.
- Published
- 2011
- Full Text
- View/download PDF
20. No to Neocosmospora: Phylogenomic and Practical Reasons for Continued Inclusion of the Fusarium solani Species Complex in the Genus Fusarium
- Author
-
O'Donnell, Kerry, Al-Hatmi, Abdullah M. S., Aoki, Takayuki, Brankovics, Balázs, Cano-Lira, José F., Coleman, Jeffrey J., de Hoog, G. Sybren, Di Pietro, Antonio, Frandsen, Rasmus J. N., Geiser, David M., Gibas, Connie F. C., Guarro, Josep, Kim, Hye-Seon, Kistler, H. Corby, Laraba, Imane, Leslie, John F., López-Berges, Manuel S, Lysøe, Erik, Meis, Jacques F., Monod, Michel, Proctor, Robert H., Rep, Martijn, Ruiz-Roldán, Carmen, Šišić, Adnan, Stajich, Jason E., Steenkamp, Emma T., Summerell, Brett A., van der Lee, Theo A. J., van Diepeningen, Anne D., Verweij, Paul E., Waalwijk, Cees, Ward, Todd J., Wickes, Brian L., Wiederhold, Nathan P., Wingfield, Michael J., Zhang, Ning, Zhang, Sean X., O'Donnell, Kerry, Al-Hatmi, Abdullah M. S., Aoki, Takayuki, Brankovics, Balázs, Cano-Lira, José F., Coleman, Jeffrey J., de Hoog, G. Sybren, Di Pietro, Antonio, Frandsen, Rasmus J. N., Geiser, David M., Gibas, Connie F. C., Guarro, Josep, Kim, Hye-Seon, Kistler, H. Corby, Laraba, Imane, Leslie, John F., López-Berges, Manuel S, Lysøe, Erik, Meis, Jacques F., Monod, Michel, Proctor, Robert H., Rep, Martijn, Ruiz-Roldán, Carmen, Šišić, Adnan, Stajich, Jason E., Steenkamp, Emma T., Summerell, Brett A., van der Lee, Theo A. J., van Diepeningen, Anne D., Verweij, Paul E., Waalwijk, Cees, Ward, Todd J., Wickes, Brian L., Wiederhold, Nathan P., Wingfield, Michael J., Zhang, Ning, and Zhang, Sean X.
- Abstract
This article is to alert medical mycologists and infectious disease specialists of recent name changes of medically important species of the filamentous mold Fusarium Fusarium species can cause localized and life-threating infections in humans. Of the 70 Fusarium species that have been reported to cause infections, close to one-third are members of the Fusarium solani species complex (FSSC), and they collectively account for approximately two-thirds of all reported Fusarium infections. Many of these species were recently given scientific names for the first time by a research group in the Netherlands, but they were misplaced in the genus Neocosmospora In this paper, we present genetic arguments that strongly support inclusion of the FSSC in Fusarium There are potentially serious consequences associated with using the name Neocosmospora for Fusarium species because clinicians need to be aware that fusaria are broadly resistant to the spectrum of antifungals that are currently available.
- Published
- 2020
21. Synchytrium endobioticum, the potato wart disease pathogen.
- Author
-
van de Vossenberg, Bart. T. L. H., Prodhomme, Charlotte, Vossen, Jack H., and van der Lee, Theo A. J.
- Subjects
WARTS ,POTATOES ,DISEASE management ,TUBERS ,SYMPTOMS ,FUNGI - Abstract
Potato wart disease is considered one of the most important quarantine pests for cultivated potato and is caused by the obligate biotrophic chytrid fungus Synchytrium endobioticum. This review integrates observations from early potato wart research and recent molecular, genetic, and genomic studies of the pathogen and its host potato. Taxonomy, epidemiology, pathology, and formation of new pathotypes are discussed, and a model for molecular S. endobioticum–potato interaction is proposed. Taxonomy: Currently classified as kingdom: Fungi, phylum: Chytridiomycota, class: Chytridiomycetes, order: Chytridiales, family: Synchytriaceae, genus: Synchytrium, species: Synchytrium endobioticum, there is strong molecular support for Synchytriaceae to be transferred to the order Synchytriales. Hosts and disease symptoms: Solanum tuberosum is the main host for S. endobioticum but other solanaceous species have been reported as alternative hosts. It is not known if these alternative hosts play a role in the survival of the pathogen in (borders of) infested fields. Disease symptoms on potato tubers are characterized by the warty cauliflower‐like malformations that are the result of cell enlargement and cell multiplication induced by the pathogen. Meristematic tissue on tubers, stolons, eyes, sprouts, and inflorescences can be infected while the potato root system seems to be immune. Pathotypes: For S. endobioticum over 40 pathotypes, which are defined as groups of isolates with a similar response to a set of differential potato varieties, are described. Pathotypes 1(D1), 2(G1), 6(O1), and 18(T1) are currently regarded to be most widespread. However, with the current differential set other pathogen diversity largely remains undetected. Pathogen–host interaction: A single effector has been described for S. endobioticum (AvrSen1), which is recognized by the potato Sen1 resistance gene product. This is also the first effector that has been described in Chytridiomycota, showing that in this fungal division resistance also fits the gene‐for‐gene concept. Although significant progress was made in the last decade in mapping wart disease resistance loci, not all resistances present in potato breeding germplasm could be identified. The use of resistant varieties plays an essential role in disease management. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. No to Neocosmospora : Phylogenomic and Practical Reasons for Continued Inclusion of the Fusarium solani Species Complex in the Genus Fusarium
- Author
-
O’Donnell, Kerry, primary, Al-Hatmi, Abdullah M. S., additional, Aoki, Takayuki, additional, Brankovics, Balázs, additional, Cano-Lira, José F., additional, Coleman, Jeffrey J., additional, de Hoog, G. Sybren, additional, Di Pietro, Antonio, additional, Frandsen, Rasmus J. N., additional, Geiser, David M., additional, Gibas, Connie F. C., additional, Guarro, Josep, additional, Kim, Hye-Seon, additional, Kistler, H. Corby, additional, Laraba, Imane, additional, Leslie, John F., additional, López-Berges, Manuel S., additional, Lysøe, Erik, additional, Meis, Jacques F., additional, Monod, Michel, additional, Proctor, Robert H., additional, Rep, Martijn, additional, Ruiz-Roldán, Carmen, additional, Šišić, Adnan, additional, Stajich, Jason E., additional, Steenkamp, Emma T., additional, Summerell, Brett A., additional, van der Lee, Theo A. J., additional, van Diepeningen, Anne D., additional, Verweij, Paul E., additional, Waalwijk, Cees, additional, Ward, Todd J., additional, Wickes, Brian L., additional, Wiederhold, Nathan P., additional, Wingfield, Michael J., additional, Zhang, Ning, additional, and Zhang, Sean X., additional
- Published
- 2020
- Full Text
- View/download PDF
23. Detecting Introgression Between Members of the Fusarium fujikuroi and F. oxysporum Species Complexes by Comparative Mitogenomics
- Author
-
Brankovics, Balázs, primary, van Diepeningen, Anne D., additional, de Hoog, G. Sybren, additional, van der Lee, Theo A. J., additional, and Waalwijk, Cees, additional
- Published
- 2020
- Full Text
- View/download PDF
24. Population Genomic Analysis Reveals a Highly Conserved Mitochondrial Genome in Fusarium asiaticum
- Author
-
Yang, Meixin, primary, Zhang, Hao, additional, van der Lee, Theo A. J., additional, Waalwijk, Cees, additional, van Diepeningen, Anne D., additional, Feng, Jie, additional, Brankovics, Balázs, additional, and Chen, Wanquan, additional
- Published
- 2020
- Full Text
- View/download PDF
25. TheSynchytrium endobioticumAvrSen1 Triggers a Hypersensitive Response inSen1Potatoes While Natural Variants Evade Detection
- Author
-
van de Vossenberg, Bart T. L. H., primary, Prodhomme, Charlotte, additional, van Arkel, Gert, additional, van Gent-Pelzer, Marga P. E., additional, Bergervoet, Marjan, additional, Brankovics, Balázs, additional, Przetakiewicz, Jarosław, additional, Visser, Richard G. F., additional, van der Lee, Theo A. J., additional, and Vossen, Jack H., additional
- Published
- 2019
- Full Text
- View/download PDF
26. Live and dead qPCR detection demonstrates that feeding of Nosema ceranaeresults in infection in the honey bee but not the bumble bee
- Author
-
van der Steen, Jozef J. M., Hendriks, Marc J. A., van Diepeningen, Anne D., van Gent-Pelzer, Marga P. E., and van der Lee, Theo A. J.
- Abstract
AbstractAs the honey bee and bumble bee may suffer from the same or related microbial pathogens, cross contamination from commercially reared Bombusspp. to honey bees and wild bumble bees and vice versa is a major concern. Honey bee-collected pollen to feed commercially reared Bombusspp. is a potential risk. Nosemaspp. is a fungal pathogen in bees. In this study, we developed new quantitative detection tools based on the detection of RNA using a TaqMan-based RT-qPCR for Nosema ceranaeand Nosema apis, with extraction controls based on the actin gene of honey bees and bumble bees, respectively. These tools were subsequently applied to study the epidemiology of N. ceranae, a main disease in honey bees. We screened gamma radiation and cold treatment sterilisation for their efficacy to kill N. ceranaespores fed in sugar water and in pollen to honey bees and bumble bees, respectively. N. ceranaeinfection in adult bumble bees was checked. Spores passing the inter-alimentary track were found but no infection was observed. N. ceranaespores were fed to honey bees. Their presence and multiplication were demonstrated, showing the spores were both viable and infectious. Our results indicate that N. ceranaefound in honey bees cannot infect commercially reared bumble bees (Bombus terrestris) and, that gamma radiation effectively kills N. ceranae. The highly specific and sensitive molecular assays developed, were exploited to detect N. ceranaein pollen and faeces, which would allow more comprehensive epidemiological studies on this important pathogen.
- Published
- 2022
- Full Text
- View/download PDF
27. Development and Evaluation of a Triplex TaqMan Assay and Next-Generation Sequence Analysis for Improved Detection of Xylella in Plant Material
- Author
-
Bonants, Peter, primary, Griekspoor, Yvonne, additional, Houwers, Ilse, additional, Krijger, Marjon, additional, van der Zouwen, Patricia, additional, van der Lee, Theo A. J., additional, and van der Wolf, Jan, additional
- Published
- 2019
- Full Text
- View/download PDF
28. Stress and sexual reproduction affect the dynamics of the wheat pathogen effector AvrStb6 and strobilurin resistance
- Author
-
Kema, Gerrit H J, Mirzadi Gohari, Amir, Aouini, Lamia, Gibriel, Hesham A Y, Ware, Sarah B, van den Bosch, Frank, Manning-Smith, Robbie, Alonso-Chavez, Vasthi, Helps, Joe, Ben M'Barek, Sarrah, Mehrabi, Rahim, Diaz-Trujillo, Caucasella, Zamani, Elham, Schouten, Henk J, van der Lee, Theo A J, Waalwijk, Cees, de Waard, Maarten A, de Wit, Pierre J G M, Verstappen, Els C P, Thomma, Bart P H J, Meijer, Harold J G, Seidl, Michael F, Kema, Gerrit H J, Mirzadi Gohari, Amir, Aouini, Lamia, Gibriel, Hesham A Y, Ware, Sarah B, van den Bosch, Frank, Manning-Smith, Robbie, Alonso-Chavez, Vasthi, Helps, Joe, Ben M'Barek, Sarrah, Mehrabi, Rahim, Diaz-Trujillo, Caucasella, Zamani, Elham, Schouten, Henk J, van der Lee, Theo A J, Waalwijk, Cees, de Waard, Maarten A, de Wit, Pierre J G M, Verstappen, Els C P, Thomma, Bart P H J, Meijer, Harold J G, and Seidl, Michael F
- Published
- 2018
29. The linear mitochondrial genome of the quarantine chytrid Synchytrium endobioticum; insights into the evolution and recent history of an obligate biotrophic plant pathogen
- Author
-
van de Vossenberg, Bart T. L. H., primary, Brankovics, Balázs, additional, Nguyen, Hai D. T., additional, van Gent-Pelzer, Marga P. E., additional, Smith, Donna, additional, Dadej, Kasia, additional, Przetakiewicz, Jarosław, additional, Kreuze, Jan F., additional, Boerma, Margriet, additional, van Leeuwen, Gerard C. M., additional, André Lévesque, C., additional, and van der Lee, Theo A. J., additional
- Published
- 2018
- Full Text
- View/download PDF
30. Evolution and Diversity of Biosynthetic Gene Clusters in Fusarium
- Author
-
Hoogendoorn, Koen, primary, Barra, Lena, additional, Waalwijk, Cees, additional, Dickschat, Jeroen S., additional, van der Lee, Theo A. J., additional, and Medema, Marnix H., additional
- Published
- 2018
- Full Text
- View/download PDF
31. The Fusarium graminearum Histone Acetyltransferases Are Important for Morphogenesis, DON Biosynthesis, and Pathogenicity
- Author
-
Kong, Xiangjiu, primary, van Diepeningen, Anne D., additional, van der Lee, Theo A. J., additional, Waalwijk, Cees, additional, Xu, Jingsheng, additional, Xu, Jin, additional, Zhang, Hao, additional, Chen, Wanquan, additional, and Feng, Jie, additional
- Published
- 2018
- Full Text
- View/download PDF
32. Mitochondrial genomes reveal recombination in the presumed asexual Fusarium oxysporum species complex
- Author
-
Brankovics, Balázs, van Dam, Peter, Rep, Martijn, de Hoog, G. S., van der Lee, Theo A J, Waalwijk, Cees, van Diepeningen, A.D., Brankovics, Balázs, van Dam, Peter, Rep, Martijn, de Hoog, G. S., van der Lee, Theo A J, Waalwijk, Cees, and van Diepeningen, A.D.
- Abstract
Background The Fusarium oxysporum species complex (FOSC) contains several phylogenetic lineages. Phylogenetic studies identified two to three major clades within the FOSC. The mitochondrial sequences are highly informative phylogenetic markers, but have been mostly neglected due to technical difficulties. Results A total of 61 complete mitogenomes of FOSC strains were de novo assembled and annotated. Length variations and intron patterns support the separation of three phylogenetic species. The variable region of the mitogenome that is typical for the genus Fusarium shows two new variants in the FOSC. The variant typical for Fusarium is found in members of all three clades, while variant 2 is found in clades 2 and 3 and variant 3 only in clade 2. The extended set of loci analyzed using a new implementation of the genealogical concordance species recognition method support the identification of three phylogenetic species within the FOSC. Comparative analysis of the mitogenomes in the FOSC revealed ongoing mitochondrial recombination within, but not between phylogenetic species. Conclusions The recombination indicates the presence of a parasexual cycle in F. oxysporum. The obstacles hindering the usage of the mitogenomes are resolved by using next generation sequencing and selective genome assemblers, such as GRAbB. Complete mitogenome sequences offer a stable basis and reference point for phylogenetic and population genetic studies.
- Published
- 2017
33. Effector discovery in the fungal wheat pathogen Zymoseptoria tritici
- Author
-
Mirzadi Gohari, Amir, Ware, Sarah B., Wittenberg, Alexander H. J., Mehrabi, Rahim, Ben M'Barek, Sarrah, Verstappen, Els C. P., van der Lee, Theo A. J., Robert, Olivier, Schouten, Henk J., de Wit, Pierre P. J. G. M., and Kema, Gert H. J.
- Subjects
Fungal Proteins ,Ascomycota ,Virulence Factors ,Gene Expression Profiling ,Genes, Fungal ,Quantitative Trait Loci ,food and beverages ,Original Articles ,Triticum - Abstract
Fungal plant pathogens, such as Zymoseptoria tritici (formerly known as Mycosphaerella graminicola), secrete repertoires of effectors to facilitate infection or trigger host defence mechanisms. The discovery and functional characterization of effectors provides valuable knowledge that can contribute to the design of new and effective disease management strategies. Here, we combined bioinformatics approaches with expression profiling during pathogenesis to identify candidate effectors of Z. tritici. In addition, a genetic approach was conducted to map quantitative trait loci (QTLs) carrying putative effectors, enabling the validation of both complementary strategies for effector discovery. In planta expression profiling revealed that candidate effectors were up-regulated in successive waves corresponding to consecutive stages of pathogenesis, contrary to candidates identified by QTL mapping that were, overall, expressed at low levels. Functional analyses of two top candidate effectors (SSP15 and SSP18) showed their dispensability for Z. tritici pathogenesis. These analyses reveal that generally adopted criteria, such as protein size, cysteine residues and expression during pathogenesis, may preclude an unbiased effector discovery. Indeed, genetic mapping of genomic regions involved in specificity render alternative effector candidates that do not match the aforementioned criteria, but should nevertheless be considered as promising new leads for effectors that are crucial for the Z. tritici-wheat pathosystem.
- Published
- 2015
34. First steps towards mitochondrial pan-genomics: detailed analysis of Fusarium graminearum mitogenomes.
- Author
-
Brankovics, Balázs, Kulik, Tomasz, Sawicki, Jakub, Bilska, Katarzyna, Hao Zhang, de Hoog, G Sybren, van der Lee, Theo A. J., Waalwijk, Cees, and van Diepeningen, Anne D.
- Subjects
FUSARIUM ,INTRONS ,NUCLEOTIDE sequencing ,DATA mining ,HORIZONTAL wells ,MITOCHONDRIAL DNA abnormalities - Abstract
There is a gradual shift from representing a species' genome by a single reference genome sequence to a pan-genome representation. Pan-genomes are the abstract representations of the genomes of all the strains that are present in the population or species. In this study, we employed a pan-genomic approach to analyze the intraspecific mitochondrial genome diversity of Fusarium graminearum. We present an improved reference mitochondrial genome for F. graminearum with an intron-exon annotation that was verified using RNA-seq data. Each of the 24 studied isolates had a distinct mitochondrial sequence. Length variation in the F. graminearum mitogenome was found to be largely due to variation of intron regions (99.98%). The ‘‘intronless’’ mitogenome length was found to be quite stable and could be informative when comparing species. The coding regions showed high conservation, while the variability of intergenic regions was highest. However, the most important variable parts are the intron regions, because they contain approximately half of the variable sites, make up more than half of the mitogenome, and show presence/absence variation. Furthermore, our analyses show that the mitogenome of F. graminearum is recombining, as was previously shown in F. oxysporum, indicating that mitogenome recombination is a common phenomenon in Fusarium. The majority of mitochondrial introns in F. graminearum belongs to group I introns, which are associated with homing endonuclease genes (HEGs). Mitochondrial introns containing HE genes may spread within populations through homing, where the endonuclease recognizes and cleaves the recognition site in the target gene. After cleavage of the ``host' gene, it is replaced by the gene copy containing the intron with HEG. We propose to use introns unique to a population for tracking the spread of the given population, because introns can spread through vertical inheritance, recombination as well as via horizontal transfer. We demonstrate how pooled sequencing of strains can be used for mining mitogenome data. The usage of pooled sequencing offers a scalable solution for population analysis and for species level comparisons studies. This study may serve as a basis for future mitochondrial genome variability studies and representations. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
35. GRAbB: Selective Assembly of Genomic Regions, a New Niche for Genomic Research
- Author
-
Brankovics, Balázs, Zhang, Hao, van Diepeningen, Anne D, van der Lee, Theo A J, Waalwijk, Cees, de Hoog, G Sybren, Brankovics, Balázs, Zhang, Hao, van Diepeningen, Anne D, van der Lee, Theo A J, Waalwijk, Cees, and de Hoog, G Sybren
- Abstract
GRAbB (Genomic Region Assembly by Baiting) is a new program that is dedicated to assemble specific genomic regions from NGS data. This approach is especially useful when dealing with multi copy regions, such as mitochondrial genome and the rDNA repeat region, parts of the genome that are often neglected or poorly assembled, although they contain interesting information from phylogenetic or epidemiologic perspectives, but also single copy regions can be assembled. The program is capable of targeting multiple regions within a single run. Furthermore, GRAbB can be used to extract specific loci from NGS data, based on homology, like sequences that are used for barcoding. To make the assembly specific, a known part of the region, such as the sequence of a PCR amplicon or a homologous sequence from a related species must be specified. By assembling only the region of interest, the assembly process is computationally much less demanding and may lead to assemblies of better quality. In this study the different applications and functionalities of the program are demonstrated such as: exhaustive assembly (rDNA region and mitochondrial genome), extracting homologous regions or genes (IGS, RPB1, RPB2 and TEF1a), as well as extracting multiple regions within a single run. The program is also compared with MITObim, which is meant for the exhaustive assembly of a single target based on a similar query sequence. GRAbB is shown to be more efficient than MITObim in terms of speed, memory and disk usage. The other functionalities (handling multiple targets simultaneously and extracting homologous regions) of the new program are not matched by other programs. The program is available with explanatory documentation at https://github.com/b-brankovics/grabb. GRAbB has been tested on Ubuntu (12.04 and 14.04), Fedora (23), CentOS (7.1.1503) and Mac OS X (10.7). Furthermore, GRAbB is available as a docker repository: brankovics/grabb (https://hub.docker.com/r/brankovics/grabb/).
- Published
- 2016
36. Crops are a main driver for species diversityand the toxigenic potential of Fusarium isolates in maize ears in China.
- Author
-
Zhang, Hao, Brankovics, Balázs, Luo, W., Xu, J., Xu, J.S., Guo, C., Guo, J.G., Jin, S.L., Chen, W.Q., Feng, J., van Diepeningen, Anne D., van der Lee, Theo A J, Waalwijk, Cees, Zhang, Hao, Brankovics, Balázs, Luo, W., Xu, J., Xu, J.S., Guo, C., Guo, J.G., Jin, S.L., Chen, W.Q., Feng, J., van Diepeningen, Anne D., van der Lee, Theo A J, and Waalwijk, Cees
- Abstract
In recent years increasing demands and the relatively low-care cultivation of the crop have resulted in an enormous expansion of the acreage of maize in China. However, particularly in China, Fusarium ear rot forms an important constraint to maize production. In this study, we showed that members of both the Fusarium fujikuroi species complex (FFSC) and the Fusarium graminearum species complex are the causal agents of Fusarium ear rot in the main maize producing areas in China. Fumonisin producing Fusarium verticillioides was the most prevalent species, followed by fumonisin producing Fusarium proliferatum and 15-acetyldeoxynivalenol producing F. graminearum. Both Fusarium temperatum and Fusarium boothii were identified for the first time in the colder regions in China, extending their known habitats to colder environments. Mating type analysis of the different heterothallic FFSC species, showed that both types co-occur in each sampling site suggestive of the possibility of sexual recombination. Virulence tests with F. boothii (from maize) and F. graminearum from maize or wheat showed adaptation to the host. In addition, F. graminearum seems to outcompete F. boothii in wheat-maize rotations. Based on our findings and previous studies, we conclude that wheat/maize rotation selects for F. graminearum, while a wheat/rice rotation selects for F. asiaticum. In contrast, F. boothii is selected when maize is cultivated without rotation. A higher occurrence of F. temperatum is observed on maize in colder climatological regions in China, while Fusarium meridionale seems restricted to mountain areas. Each of these species has their characteristic mycotoxin profile and deoxynivalenol and fumonisin are the potential threats to maize production in Northern China.
- Published
- 2016
37. Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control
- Author
-
Arango Isaza, Rafael E., Diaz-Trujillo, Caucasella, Dhillon, Braham, Aerts, Andrea, Carlier, Jean, Crane, Charles F., V. de Jong, Tristan, de Vries, Ineke, Dietrich, Robert, Farmer, Andrew D., Fortes Fereira, Claudia, Garcia, Suzana, Guzman, Mauricio, Hamelin, Richard C., Lindquist, Erika A., Mehrabi, Rahim, Quiros, Olman, Schmutz, Jeremy, Shapiro, Harris, Reynolds, Elizabeth, Scalliet, Gabriel, Souza Manoel, Jr., Stergiopoulos, Ioannis, Van der Lee, Theo A. J., De Wit, Pierre J. G. M., Zapater, Marie-Françoise, Zwiers, Lute-Harm, Grigoriev, Igor V., Goodwin, Stephen B., Kema, Gert H. J., Arango Isaza, Rafael E., Diaz-Trujillo, Caucasella, Dhillon, Braham, Aerts, Andrea, Carlier, Jean, Crane, Charles F., V. de Jong, Tristan, de Vries, Ineke, Dietrich, Robert, Farmer, Andrew D., Fortes Fereira, Claudia, Garcia, Suzana, Guzman, Mauricio, Hamelin, Richard C., Lindquist, Erika A., Mehrabi, Rahim, Quiros, Olman, Schmutz, Jeremy, Shapiro, Harris, Reynolds, Elizabeth, Scalliet, Gabriel, Souza Manoel, Jr., Stergiopoulos, Ioannis, Van der Lee, Theo A. J., De Wit, Pierre J. G. M., Zapater, Marie-Françoise, Zwiers, Lute-Harm, Grigoriev, Igor V., Goodwin, Stephen B., and Kema, Gert H. J.
- Abstract
Black Sigatoka or black leaf streak disease, caused by the ascomycete fungus Pseudocercospora fijiensis, inflicts huge costs on banana producers, due to crop losses and expenses for disease control. The global banana export trade relies on Cavendish clones that are highly susceptible to P. fijiensis. Sustainable production of the world’s number one fruit requires a better understanding of host resistance and sophisticated management of fungicide resistance in the pathogen. In the P. fijiensis genome sequence we identified an effector that induced an HR-like necrosis on a resistant banana accession but not on a susceptible cultivar. If validated, this assay may be useful for identifying resistance in banana breeding programs. We also used the genomic sequence to develop highly polymorphic molecular markers for analyzing P. fijiensis field populations and identified a strong enrichment (nearly 100%) for fungicide resistance markers in fungicide-treated banana plantations compared to untreated wild-type populations. This rapid evolution of fungicide resistance poses an immediate threat to sustainable banana production.
- Published
- 2016
38. Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control
- Author
-
Arango-Isaza, Rafael E, Diaz-Trujillo, Caucasella, Dhillon, Braham Deep Singh, Aerts, Andrea L, Carlier, Jean, Crane, Charles F., De Jong, Tristan V., De Vries, Ineke M, Dietrich, Robert A, Farmer, Andrew D, Fortes Fereira, Claudia, Garcia, Suzana A L, Guzmán, Mauricio, Hamelin, Richard C, Lindquist, Erika A, Mehrabi, Rahim, Quiros, Olman, Schmultz, Jeremy, Shapiro, Harris J., Reynolds, Elizabeth, Scalliet, Gabriel, Souza, Manoel Teixeira, Stergiopoulos, Ioannis, Van Der Lee, Theo A J, De Wit, Pierre J G M, Zapater, Marie Françoise, Zwiers, Lute Harm, Grigoriev, Igor V., Goodwin, Stephen B., Kema, Gert Hj J, Arango-Isaza, Rafael E, Diaz-Trujillo, Caucasella, Dhillon, Braham Deep Singh, Aerts, Andrea L, Carlier, Jean, Crane, Charles F., De Jong, Tristan V., De Vries, Ineke M, Dietrich, Robert A, Farmer, Andrew D, Fortes Fereira, Claudia, Garcia, Suzana A L, Guzmán, Mauricio, Hamelin, Richard C, Lindquist, Erika A, Mehrabi, Rahim, Quiros, Olman, Schmultz, Jeremy, Shapiro, Harris J., Reynolds, Elizabeth, Scalliet, Gabriel, Souza, Manoel Teixeira, Stergiopoulos, Ioannis, Van Der Lee, Theo A J, De Wit, Pierre J G M, Zapater, Marie Françoise, Zwiers, Lute Harm, Grigoriev, Igor V., Goodwin, Stephen B., and Kema, Gert Hj J
- Abstract
Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this pathog
- Published
- 2016
39. Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control
- Author
-
Arango Isaza, Rafael E., primary, Diaz-Trujillo, Caucasella, additional, Dhillon, Braham, additional, Aerts, Andrea, additional, Carlier, Jean, additional, Crane, Charles F., additional, V. de Jong, Tristan, additional, de Vries, Ineke, additional, Dietrich, Robert, additional, Farmer, Andrew D., additional, Fortes Fereira, Claudia, additional, Garcia, Suzana, additional, Guzman, Mauricio, additional, Hamelin, Richard C., additional, Lindquist, Erika A., additional, Mehrabi, Rahim, additional, Quiros, Olman, additional, Schmutz, Jeremy, additional, Shapiro, Harris, additional, Reynolds, Elizabeth, additional, Scalliet, Gabriel, additional, Souza, Manoel, additional, Stergiopoulos, Ioannis, additional, Van der Lee, Theo A. J., additional, De Wit, Pierre J. G. M., additional, Zapater, Marie-Françoise, additional, Zwiers, Lute-Harm, additional, Grigoriev, Igor V., additional, Goodwin, Stephen B., additional, and Kema, Gert H. J., additional
- Published
- 2016
- Full Text
- View/download PDF
40. GRAbB: Selective Assembly of Genomic Regions, a New Niche for Genomic Research
- Author
-
Brankovics, Balázs, primary, Zhang, Hao, additional, van Diepeningen, Anne D., additional, van der Lee, Theo A. J., additional, Waalwijk, Cees, additional, and de Hoog, G. Sybren, additional
- Published
- 2016
- Full Text
- View/download PDF
41. A gapless genome sequence of the fungusBotrytis cinerea
- Author
-
Van Kan, Jan A. L., primary, Stassen, Joost H. M., additional, Mosbach, Andreas, additional, Van Der Lee, Theo A. J., additional, Faino, Luigi, additional, Farmer, Andrew D., additional, Papasotiriou, Dimitrios G., additional, Zhou, Shiguo, additional, Seidl, Michael F., additional, Cottam, Eleanor, additional, Edel, Dominique, additional, Hahn, Matthias, additional, Schwartz, David C., additional, Dietrich, Robert A., additional, Widdison, Stephanie, additional, and Scalliet, Gabriel, additional
- Published
- 2016
- Full Text
- View/download PDF
42. Development of Polymorphic Microsatellite Loci for Potato Wart from Next-Generation Sequence Data
- Author
-
Gagnon, Marie-Claude, primary, van der Lee, Theo A. J., additional, Bonants, Peter J. M., additional, Smith, Donna S., additional, Li, Xiang, additional, Lévesque, C. André, additional, and Bilodeau, Guillaume J., additional
- Published
- 2016
- Full Text
- View/download PDF
43. Effector discovery in the fungal wheat pathogenZymoseptoria tritici
- Author
-
Mirzadi Gohari, Amir, primary, Ware, Sarah B., additional, Wittenberg, Alexander H. J., additional, Mehrabi, Rahim, additional, Ben M'Barek, Sarrah, additional, Verstappen, Els C. P., additional, van der Lee, Theo A. J., additional, Robert, Olivier, additional, Schouten, Henk J., additional, de Wit, Pierre P. J. G. M., additional, and Kema, Gert H. J., additional
- Published
- 2015
- Full Text
- View/download PDF
44. A gapless genome sequence of the fungus Botrytis cinerea.
- Author
-
Van Kan, Jan A. L., Stassen, Joost H. M., Mosbach, Andreas, Van Der Lee, Theo A. J., Faino, Luigi, Farmer, Andrew D., Papasotiriou, Dimitrios G., Zhou, Shiguo, Seidl, Michael F., Cottam, Eleanor, Edel, Dominique, Hahn, Matthias, Schwartz, David C., Dietrich, Robert A., Widdison, Stephanie, and Scalliet, Gabriel
- Subjects
BOTRYTIS cinerea ,NUCLEOTIDE sequence ,FUNGAL genetics ,GENE mapping ,FUNGICIDE resistance - Abstract
Following earlier incomplete and fragmented versions of a genome sequence for the grey mould Botrytis cinerea, a gapless, near-finished genome sequence for B. cinerea strain B05.10 is reported. The assembly comprised 18 chromosomes and was confirmed by an optical map and a genetic map based on approximately 75 000 single nucleotide polymorphism (SNP) markers. All chromosomes contained fully assembled centromeric regions, and 10 chromosomes had telomeres on both ends. The genetic map consisted of 4153 cM and a comparison of the genetic distances with the physical distances identified 40 recombination hotspots. The linkage map also identified two mutations, located in the previously described genes Bos1 and BcsdhB, that conferred resistance to the fungicides boscalid and iprodione. The genome was predicted to encode 11 701 proteins. RNAseq data from >20 different samples were used to validate and improve gene models. Manual curation of chromosome 1 revealed interesting features, such as the occurrence of a dicistronic transcript and fully overlapping genes in opposite orientations, as well as many spliced antisense transcripts. Manual curation also revealed that the untranslated regions (UTRs) of genes can be complex and long, with many UTRs exceeding lengths of 1 kb and possessing multiple introns. Community annotation is in progress. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
45. Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis
- Author
-
Goodwin, Stephen B., M’Barek, Sarrah Ben, Dhillon, Braham, Wittenberg, Alexander H. J., Crane, Charles F., Hane, James K., Foster, Andrew J., Van der Lee, Theo A. J., Grimwood, Jane, Aerts, Andrea, Antoniw, John, Bailey, Andy, Bluhm, Burt, Bowler, Judith, Bristow, Jim, van der Burgt, Ate, Canto-Canche, Blondy, Churchill, Alice C. L., Conde-Ferraez, Laura, Cools, Hans J., Coutinho, Pedro M., Csukai, Michael, Dehal, Paramvir, De Wit, Pierre, Donzelli, Bruno, van de Geest, Henri C., van Ham, Roel C. H. J., Hammond-Kosack, Kim E., Henrissat, Bernard, Kilian, Andrzej, Kobayashi, Adilson K., Koopmann, Edda, Kourmpetis, Yiannis, Kuzniar, Arnold, Lindquist, Erika, Lombard, Vincent, Maliepaard, Chris, Martins, Natalia, Mehrabi, Rahim, Nap, Jan P. H., Ponomarenko, Alisa, Rudd, Jason J., Salamov, Asaf, Schmutz, Jeremy, Schouten, Henk J., Shapiro, Harris, Stergiopoulos, Ioannis, Torriani, Stefano F.F., Tu, Hank, de Vries, Ronald P., Waalwijk, Cees, Ware, Sarah B., Wiebenga, Ad, Zwiers, Lute-Harm, Oliver, Richard P., Grigoriev, Igor V., Kema, Gert. H. J., Goodwin, Stephen B., M’Barek, Sarrah Ben, Dhillon, Braham, Wittenberg, Alexander H. J., Crane, Charles F., Hane, James K., Foster, Andrew J., Van der Lee, Theo A. J., Grimwood, Jane, Aerts, Andrea, Antoniw, John, Bailey, Andy, Bluhm, Burt, Bowler, Judith, Bristow, Jim, van der Burgt, Ate, Canto-Canche, Blondy, Churchill, Alice C. L., Conde-Ferraez, Laura, Cools, Hans J., Coutinho, Pedro M., Csukai, Michael, Dehal, Paramvir, De Wit, Pierre, Donzelli, Bruno, van de Geest, Henri C., van Ham, Roel C. H. J., Hammond-Kosack, Kim E., Henrissat, Bernard, Kilian, Andrzej, Kobayashi, Adilson K., Koopmann, Edda, Kourmpetis, Yiannis, Kuzniar, Arnold, Lindquist, Erika, Lombard, Vincent, Maliepaard, Chris, Martins, Natalia, Mehrabi, Rahim, Nap, Jan P. H., Ponomarenko, Alisa, Rudd, Jason J., Salamov, Asaf, Schmutz, Jeremy, Schouten, Henk J., Shapiro, Harris, Stergiopoulos, Ioannis, Torriani, Stefano F.F., Tu, Hank, de Vries, Ronald P., Waalwijk, Cees, Ware, Sarah B., Wiebenga, Ad, Zwiers, Lute-Harm, Oliver, Richard P., Grigoriev, Igor V., and Kema, Gert. H. J.
- Abstract
The plant-pathogenic fungus Mycosphaerella graminicola (asexual stage: Septoria tritici) causes septoria tritici blotch, a disease that greatly reduces the yield and quality of wheat. This disease is economically important in most wheat-growing areas worldwide and threatens global food production. Control of the disease has been hampered by a limited understanding of the genetic and biochemical bases of pathogenicity, including mechanisms of infection and of resistance in the host. Unlike most other plant pathogens, M. graminicola has a long latent period during which it evades host defenses. Although this type of stealth pathogenicity occurs commonly in Mycosphaerella and other Dothideomycetes, the largest class of plant-pathogenic fungi, its genetic basis is not known. To address this problem, the genome of M. graminicola was sequenced completely. The finished genome contains 21 chromosomes, eight of which could be lost with no visible effect on the fungus and thus are dispensable. This eight-chromosome dispensome is dynamic in field and progeny isolates, is different from the core genome in gene and repeat content, and appears to have originated by ancient horizontal transfer from an unknown donor. Synteny plots of the M. graminicola chromosomes versus those of the only other sequenced Dothideomycete, Stagonospora nodorum, revealed conservation of gene content but not order or orientation, suggesting a high rate of intra-chromosomal rearrangement in one or both species.This observed “mesosynteny” is very different from synteny seen between other organisms. A surprising feature of the M. graminicola genome compared to other sequenced plant pathogens was that it contained very few genes for enzymes that break down plant cell walls, which was more similar to endophytes than to pathogens. The stealth pathogenesis of M. graminicola probably involves degradation of proteins rather than carbohydrates to evade host defenses during the biotrophic stage of infection and ma
- Published
- 2011
46. The het-c heterokaryon incompatibility gene in Aspergillus niger
- Author
-
van Diepeningen, Anne D, Pál, Károly, van der Lee, Theo A J, Hoekstra, Rolf F, Debets, Alfons J M, van Diepeningen, Anne D, Pál, Károly, van der Lee, Theo A J, Hoekstra, Rolf F, and Debets, Alfons J M
- Abstract
Heterokaryon incompatibility among Aspergillus niger strains is a widespread phenomenon that is observed as the inability to form stable heterokaryons. The genetic basis of heterokaryon incompatibility reactions is well established in some sexual filamentous fungi but largely unknown in presumed asexual species, such as A. niger. To test whether the genes that determine heterokaryon incompatibility in Neurospora crassa, such as het-c, vib-1 and pin-c, have a similar function in A. niger, we performed a short in silico search for homologues of these genes in the A. niger and several related genomes. For het-c, pin-c and vib-1 we did indeed identify putative orthologues. We then screened a genetically diverse worldwide collection of incompatible black Aspergilli for polymorphisms in the het-c orthologue. No size variation was observed in the variable het-c indel region that determines the specificity in N. crassa. Sequence comparison showed only minor variation in the number of glutamine coding triplets. However, introduction of one of the three N. crassa alleles (het-c2) in A. niger by transformation resulted in an abortive phenotype, reminiscent of the heterokaryon incompatibility in N. crassa. We conclude that although the genes required are present and the het-c homologue could potentially function as a heterokaryon incompatibility gene, het-c has no direct function in heterokaryon incompatibility in A. niger because the necessary allelic variation is absent.
- Published
- 2009
47. Meiosis Drives Extraordinary Genome Plasticity in the Haploid Fungal Plant Pathogen Mycosphaerella graminicola
- Author
-
Wittenberg, Alexander H.J., Van Der Lee, Theo A. J., M'Barek, Sarrah Ben, Ware, Sarah B, Goodwin, Stephen B, Kilian, Andrzej, Visser, Richard G.F., Kema, Gert H.J., Schouten, Henk J, Wittenberg, Alexander H.J., Van Der Lee, Theo A. J., M'Barek, Sarrah Ben, Ware, Sarah B, Goodwin, Stephen B, Kilian, Andrzej, Visser, Richard G.F., Kema, Gert H.J., and Schouten, Henk J
- Abstract
Meiosis in the haploid plant-pathogenic fungus Mycosphaerella graminicola results in eight ascospores due to a mitotic division following the two meiotic divisions. The transient diploid phase allows for recombination among homologous chromosomes. However, some chromosomes of M. graminicola lack homologs and do not pair during meiosis. Because these chromosomes are not present universally in the genome of the organism they can be considered to be dispensable. To analyze the meiotic transmission of unequal chromosome numbers, two segregating populations were generated by crossing genetically unrelated parent isolates originating from Algeria and The Netherlands that had pathogenicity towards durum or bread wheat, respectively. Detailed genetic analyses of these progenies using high-density mapping (1793 DArT, 258 AFLP and 25 SSR markers) and graphical genotyping revealed that M. graminicola has up to eight dispensable chromosomes, the highest number reported in filamentous fungi. These chromosomes vary from 0.39 to 0.77 Mb in size, and represent up to 38% of the chromosomal complement. Chromosome numbers among progeny isolates varied widely, with some progeny missing up to three chromosomes, while other strains were disomic for one or more chromosomes. Between 15–20% of the progeny isolates lacked one or more chromosomes that were present in both parents. The two high-density maps showed no recombination of dispensable chromosomes and hence, their meiotic processing may require distributive disjunction, a phenomenon that is rarely observed in fungi. The maps also enabled the identification of individual twin isolates from a single ascus that shared the same missing or doubled chromosomes indicating that the chromosomal polymorphisms were mitotically stable and originated from nondisjunction during the second division and, less frequently, during the first division of fungal meiosis. High genome plasticity could be among the strategies enabling this versatile pathogen
- Published
- 2009
48. New broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat
- Author
-
Tabib Ghaffary, S. Mahmod, primary, Faris, Justin D., additional, Friesen, Timothy L., additional, Visser, Richard G. F., additional, van der Lee, Theo A. J., additional, Robert, Olivier, additional, and Kema, Gert H. J., additional
- Published
- 2011
- Full Text
- View/download PDF
49. Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis
- Author
-
Goodwin, Stephen B., primary, Ben M'Barek, Sarrah, additional, Dhillon, Braham, additional, Wittenberg, Alexander H. J., additional, Crane, Charles F., additional, Hane, James K., additional, Foster, Andrew J., additional, Van der Lee, Theo A. J., additional, Grimwood, Jane, additional, Aerts, Andrea, additional, Antoniw, John, additional, Bailey, Andy, additional, Bluhm, Burt, additional, Bowler, Judith, additional, Bristow, Jim, additional, van der Burgt, Ate, additional, Canto-Canché, Blondy, additional, Churchill, Alice C. L., additional, Conde-Ferràez, Laura, additional, Cools, Hans J., additional, Coutinho, Pedro M., additional, Csukai, Michael, additional, Dehal, Paramvir, additional, De Wit, Pierre, additional, Donzelli, Bruno, additional, van de Geest, Henri C., additional, van Ham, Roeland C. H. J., additional, Hammond-Kosack, Kim E., additional, Henrissat, Bernard, additional, Kilian, Andrzej, additional, Kobayashi, Adilson K., additional, Koopmann, Edda, additional, Kourmpetis, Yiannis, additional, Kuzniar, Arnold, additional, Lindquist, Erika, additional, Lombard, Vincent, additional, Maliepaard, Chris, additional, Martins, Natalia, additional, Mehrabi, Rahim, additional, Nap, Jan P. H., additional, Ponomarenko, Alisa, additional, Rudd, Jason J., additional, Salamov, Asaf, additional, Schmutz, Jeremy, additional, Schouten, Henk J., additional, Shapiro, Harris, additional, Stergiopoulos, Ioannis, additional, Torriani, Stefano F. F., additional, Tu, Hank, additional, de Vries, Ronald P., additional, Waalwijk, Cees, additional, Ware, Sarah B., additional, Wiebenga, Ad, additional, Zwiers, Lute-Harm, additional, Oliver, Richard P., additional, Grigoriev, Igor V., additional, and Kema, Gert H. J., additional
- Published
- 2011
- Full Text
- View/download PDF
50. Gα and Gβ Proteins Regulate the Cyclic AMP Pathway That Is Required for Development and Pathogenicity of the Phytopathogen Mycosphaerella graminicola
- Author
-
Mehrabi, Rahim, primary, M'Barek, Sarrah Ben, additional, van der Lee, Theo A. J., additional, Waalwijk, Cees, additional, de Wit, Pierre J. G. M., additional, and Kema, Gerrit H. J., additional
- Published
- 2009
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.