Search

Your search keyword '"van der Torre, Jaco"' showing total 36 results
Start Over Author "van der Torre, Jaco"
36 results on '"van der Torre, Jaco"'

9. Telomere protein arrays stall DNA loop extrusion by condensin

Author

Analikwu, Brian T, primary, Deshayes, Alice, additional, van der Torre, Jaco, additional, Guérin, Thomas, additional, Katan, Allard J, additional, Béneut, Claire, additional, Barth, Roman, additional, Phipps, Jamie, additional, Scolari, Vittore, additional, Veaute, Xavier, additional, Barrington, Christopher, additional, Busso, Dider, additional, Uhlmann, Frank, additional, Dubrana, Karine, additional, Mattarocci, Stefano, additional, Dekker, Cees, additional, and Marcand, Stéphane, additional

Published
2023
Full Text
View/download PDF

15. ParB proteins can bypass DNA-bound roadblocks via dimer-dimer recruitment

Author

Tišma, Miloš, Panoukidou, Maria, Antar, Hammam, Soh, Young-Min, Barth, Roman, Pradhan, Biswajit, Barth, Anders, van der Torre, Jaco, Michieletto, Davide, Gruber, Stephan, and Dekker, Cees

Subjects
DNA, Bacterial, Bacterial Proteins, Chromosome Segregation, DNA, Bacillus subtilis/genetics, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, DNA/metabolism, DNA, Bacterial/genetics, DNA, Bacterial/metabolism, Protein Binding, Bacillus subtilis
Abstract

The ParABS system is essential for prokaryotic chromosome segregation. After loading at parS on the genome, ParB (partition protein B) proteins rapidly redistribute to distances of ~15 kilobases from the loading site. It has remained puzzling how this large-distance spreading can occur along DNA loaded with hundreds of proteins. Using in vitro single-molecule fluorescence imaging, we show that ParB from Bacillus subtilis can load onto DNA distantly of parS, as loaded ParB molecules themselves are found to be able to recruit additional ParB proteins from bulk. Notably, this recruitment can occur in cis but also in trans, where, at low tensions within the DNA, newly recruited ParB can bypass roadblocks as it gets loaded to spatially proximal but genomically distant DNA regions. The data are supported by molecular dynamics simulations, which show that cooperative ParB-ParB recruitment can enhance spreading. ParS-independent recruitment explains how ParB can cover substantial genomic distance during chromosome segregation, which is vital for the bacterial cell cycle.

Published
2022

18. MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection

Author

Boersma, Vera, Moatti, Nathalie, Segura-Bayona, Sandra, Peuscher, Marieke H., van der Torre, Jaco, Wevers, Brigitte A., and Orthwein, Alexandre

Subjects
DNA repair -- Analysis, Genetic research, DNA damage -- Analysis, Telomeres -- Research, DNA binding proteins -- Analysis, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

MAD2L2 regulates DNA repair at deprotected telomeres and at ionizing-radiation-induced double-stranded DNA breaks by inhibiting resection of the 5' ends; the ends are thus shunted into the non-homologous end-joining pathway. MAD2L2/REV7 promotes genome integrity DNA polymerase [zeta], composed of REV3, REV7 and an associated factor, REV1, mediates a type of DNA repair involving translesion synthesis, and hence its activity is highly mutagenic. Two studies exploring the DNA damage response have converged on REV7 (also known as MAD2L2) as a factor that, by itself, can promote maintenance of genome integrity. Several protective mechanisms that prevent telomere ends being recognized as a double-strand breaks (DSBs) and triggering an inappropriate DNA damage response were known. Jacqueline Jacobs and colleagues now show that REV7/MAD2L2 suppresses homology-dependent repair at deprotected telomeres and at irradiation-induced DSBs by inhibiting resection of the 5' end. As a consequence, the ends are shunted into the non-homologous end-joining pathway. Sven Rottenberg and colleagues came to a similar conclusion by studying the development of resistance to PARP inhibitors. They found that REV7/MAD2L2 dictates pathway choice in BRCA-deficient cells and during immunoglobulin class switching. Appropriate repair of DNA lesions and the inhibition of DNA repair activities at telomeres are crucial to prevent genomic instability. By fuelling the generation of genetic alterations and by compromising cell viability, genomic instability is a driving force in cancer and ageing.sup.1,2. Here we identify MAD2L2 (also known as MAD2B or REV7) through functional genetic screening as a novel factor controlling DNA repair activities at mammalian telomeres. We show that MAD2L2 accumulates at uncapped telomeres and promotes non-homologous end-joining (NHEJ)-mediated fusion of deprotected chromosome ends and genomic instability. MAD2L2 depletion causes elongated 3' telomeric overhangs, indicating that MAD2L2 inhibits 5' end resection. End resection blocks NHEJ while committing to homology-directed repair, and is under the control of 53BP1, RIF1 and PTIP.sup.3. Consistent with MAD2L2 promoting NHEJ-mediated telomere fusion by inhibiting 5' end resection, knockdown of the nucleases CTIP or EXO1 partially restores telomere-driven genomic instability in MAD2L2-depleted cells. Control of DNA repair by MAD2L2 is not limited to telomeres. MAD2L2 also accumulates and inhibits end resection at irradiation-induced DNA double-strand breaks and promotes end-joining of DNA double-strand breaks in several settings, including during immunoglobulin class switch recombination. These activities of MAD2L2 depend on ATM kinase activity, RNF8, RNF168, 53BP1 and RIF1, but not on PTIP, REV1 and REV3, the latter two acting with MAD2L2 in translesion synthesis.sup.4. Together, our data establish MAD2L2 as a crucial contributor to the control of DNA repair activity by 53BP1 that promotes NHEJ by inhibiting 5' end resection downstream of RIF1., Author(s): Vera Boersma [sup.1] , Nathalie Moatti [sup.1] , Sandra Segura-Bayona [sup.1] , Marieke H. Peuscher [sup.1] , Jaco van der Torre [sup.1] , Brigitte A. Wevers [sup.1] , Alexandre [...]

Published
2015
Full Text
View/download PDF

30. Direct observation of DNA knots using a solid-state nanopore

Author

Plesa, Calin, primary, Verschueren, Daniel, additional, Pud, Sergii, additional, van der Torre, Jaco, additional, Ruitenberg, Justus W., additional, Witteveen, Menno J., additional, Jonsson, Magnus P., additional, Grosberg, Alexander Y., additional, Rabin, Yitzhak, additional, and Dekker, Cees, additional

Published
2016
Full Text
View/download PDF

35. Oncolytic Adenovirus Expressing a p53 Variant Resistant to Degradation by HPV E6 Protein Exhibits Potent and Selective Replication in Cervical Cancer

Author

Heideman, Daniëlle A.M., primary, Steenbergen, Renske D.M., additional, van der Torre, Jaco, additional, Scheffner, Martin, additional, Alemany, Ramon, additional, Gerritsen, Winald R., additional, Meijer, Chris J.L.M., additional, Snijders, Peter J.F., additional, and van Beusechem, Victor W., additional

Published
2005
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results