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Your search keyword '"van der Woude, Patricia"' showing total 12 results
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12 results on '"van der Woude, Patricia"'

1. Identification of IOMA-class neutralizing antibodies targeting the CD4-binding site on the HIV-1 envelope glycoprotein

Author

van Schooten, Jelle, Farokhi, Elinaz, Schorcht, Anna, van den Kerkhof, Tom L. G. M., Gao, Hongmei, van der Woude, Patricia, Burger, Judith A., Meesters, Tim G. Rijkhold, Bijl, Tom, Ghalaiyini, Riham, Turner, Hannah L., Dorning, Jessica, van Schaik, Barbera D. C., van Kampen, Antoine H. C., Labranche, Celia C., Stanfield, Robyn L., Sok, Devin, Montefiori, David C., Burton, Dennis R., Seaman, Michael S., Ozorowski, Gabriel, Wilson, Ian A., Sanders, Rogier W., Ward, Andrew B., and van Gils, Marit J.

Published
2022
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2. Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence

Author

Sliepen, Kwinten, Han, Byung Woo, Bontjer, Ilja, Mooij, Petra, Garces, Fernando, Behrens, Anna-Janina, Rantalainen, Kimmo, Kumar, Sonu, Sarkar, Anita, Brouwer, Philip J. M., Hua, Yuanzi, Tolazzi, Monica, Schermer, Edith, Torres, Jonathan L., Ozorowski, Gabriel, van der Woude, Patricia, de la Peña, Alba Torrents, van Breemen, Mariëlle J., Camacho-Sánchez, Juan Miguel, Burger, Judith A., Medina-Ramírez, Max, González, Nuria, Alcami, Jose, LaBranche, Celia, Scarlatti, Gabriella, van Gils, Marit J., Crispin, Max, Montefiori, David C., Ward, Andrew B., Koopman, Gerrit, Moore, John P., Shattock, Robin J., Bogers, Willy M., Wilson, Ian A., and Sanders, Rogier W.

Published
2019
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3. Complementary antibody lineages achieve neutralization breadth in an HIV-1 infected elite neutralizer

Author

van Schooten, Jelle, primary, Schorcht, Anna, additional, Farokhi, Elinaz, additional, Umotoy, Jeffrey C., additional, Gao, Hongmei, additional, van den Kerkhof, Tom L. G. M., additional, Dorning, Jessica, additional, Rijkhold Meesters, Tim G., additional, van der Woude, Patricia, additional, Burger, Judith A., additional, Bijl, Tom, additional, Ghalaiyini, Riham, additional, Torrents de la Peña, Alba, additional, Turner, Hannah L., additional, Labranche, Celia C., additional, Stanfield, Robyn L., additional, Sok, Devin, additional, Schuitemaker, Hanneke, additional, Montefiori, David C., additional, Burton, Dennis R., additional, Ozorowski, Gabriel, additional, Seaman, Michael S., additional, Wilson, Ian A., additional, Sanders, Rogier W., additional, Ward, Andrew B., additional, and van Gils, Marit J., additional

Published
2022
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4. Antibody responses induced by SHIV infection are more focused than those induced by soluble native HIV-1 envelope trimers in non-human primates

Author

van Schooten, Jelle, primary, van Haaren, Marlies M., additional, Li, Hui, additional, McCoy, Laura E., additional, Havenar-Daughton, Colin, additional, Cottrell, Christopher A., additional, Burger, Judith A., additional, van der Woude, Patricia, additional, Helgers, Leanne C., additional, Tomris, Ilhan, additional, Labranche, Celia C., additional, Montefiori, David C., additional, Ward, Andrew B., additional, Burton, Dennis R., additional, Moore, John P., additional, Sanders, Rogier W., additional, Crotty, Shane, additional, Shaw, George M., additional, and van Gils, Marit J., additional

Published
2021
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5. Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike

Author

Derking, Ronald, primary, Allen, Joel D., additional, Cottrell, Christopher A., additional, Sliepen, Kwinten, additional, Seabright, Gemma E., additional, Lee, Wen-Hsin, additional, Aldon, Yoann, additional, Rantalainen, Kimmo, additional, Antanasijevic, Aleksandar, additional, Copps, Jeffrey, additional, Yasmeen, Anila, additional, Cupo, Albert, additional, Cruz Portillo, Victor M., additional, Poniman, Meliawati, additional, Bol, Niki, additional, van der Woude, Patricia, additional, de Taeye, Steven W., additional, van den Kerkhof, Tom L.G.M., additional, Klasse, P.J., additional, Ozorowski, Gabriel, additional, van Gils, Marit J., additional, Moore, John P., additional, Ward, Andrew B., additional, Crispin, Max, additional, and Sanders, Rogier W., additional

Published
2021
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6. Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

Author

Cottrell, Christopher A., primary, van Schooten, Jelle, additional, Bowman, Charles A., additional, Yuan, Meng, additional, Oyen, David, additional, Shin, Mia, additional, Morpurgo, Robert, additional, van der Woude, Patricia, additional, van Breemen, Mariëlle, additional, Torres, Jonathan L., additional, Patel, Raj, additional, Gross, Justin, additional, Sewall, Leigh M., additional, Copps, Jeffrey, additional, Ozorowski, Gabriel, additional, Nogal, Bartek, additional, Sok, Devin, additional, Rakasz, Eva G., additional, Labranche, Celia, additional, Vigdorovich, Vladimir, additional, Christley, Scott, additional, Carnathan, Diane G., additional, Sather, D. Noah, additional, Montefiori, David, additional, Silvestri, Guido, additional, Burton, Dennis R., additional, Moore, John P., additional, Wilson, Ian A., additional, Sanders, Rogier W., additional, Ward, Andrew B., additional, and van Gils, Marit J., additional

Published
2020
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7. Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike

Author

Derking, Ronald, primary, Allen, Joel D., additional, Cottrell, Christopher A., additional, Sliepen, Kwinten, additional, Seabright, Gemma E., additional, Lee, Wen-Hsin, additional, Rantalainen, Kimmo, additional, Antanasijevic, Aleksandar, additional, Copps, Jeffrey, additional, Yasmeen, Anila, additional, van der Woude, Patricia, additional, de Taeye, Steven W., additional, van den Kerkhof, Tom L.G.M., additional, Klasse, P.J., additional, Ozorowski, Gabriel, additional, van Gils, Marit J., additional, Moore, John P., additional, Ward, Andrew B., additional, Crispin, Max, additional, and Sanders, Rogier W., additional

Published
2020
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8. Stabilization of the gp120 V3 loop through hydrophobic interactions reduces the immunodominant V3-directed non-neutralizing response to HIV-1 envelope trimers

Author

de Taeye, Steven W., Torrents de la Peña, Alba, Vecchione, Andrea, Scutigliani, Enzo, Sliepen, Kwinten, Burger, Judith A., van der Woude, Patricia, Schorcht, Anna, Schermer, Edith E., van Gils, Marit J., LaBranche, Celia C., Montefiori, David C., Wilson, Ian A., Moore, John P., Ward, Andrew B., Sanders, Rogier W., Graduate School, AII - Infectious diseases, Medical Microbiology and Infection Prevention, AII - Amsterdam institute for Infection and Immunity, and Other departments

Abstract

To provide protective immunity against circulating primary HIV-1 strains, a vaccine most likely has to induce broadly neutralizing antibodies to the HIV-1 envelope glycoprotein (Env) spike. Recombinant Env trimers such as the prototype BG505 SOSIP.664 that closely mimic the native Env spike can induce autologous neutralizing antibodies (NAbs) against relatively resistant (tier 2) primary viruses. Ideally, Env immunogens should present broadly neutralizing antibody epitopes but limit the presentation of immunodominant non-NAb epitopes that might induce off-target and potentially interfering responses. The V3 loop in gp120 is such a non-NAb epitope that can effectively elicit non-NAbs when animals are immunized with SOSIP.664 trimers. V3 immunogenicity can be diminished, but not abolished, by reducing the conformational flexibility of trimers via targeted sequence changes, including an A316W substitution in V3, that create the SOSIP.v4.1 and SOSIP.v5.2 variants. Here, we further modified these trimer designs by introducing leucine residues at V3 positions 306 and 308 to create hydrophobic interactions with the tryptophan residue at position 316 and with other topologically proximal sites in the V1V2 domain. Together, these modifications further stabilized the resulting SOSIP.v5.2 S306L/R308L trimers in the prefusion state in which V3 is sequestered. When we tested these trimers as immunogens in rabbits, the induction of V3 non-NAbs was significantly reduced compared with the SOSIP.v5.2 trimers and even more so compared with the SOSIP.664 prototype, without affecting the autologous NAb response. Hence, these additional trimer sequence modifications may be beneficial for immunization strategies that seek to minimize off-target non-NAb responses

Published
2018

9. Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo

Author

Medina-Ramírez, Max, primary, Garces, Fernando, additional, Escolano, Amelia, additional, Skog, Patrick, additional, de Taeye, Steven W., additional, Del Moral-Sanchez, Ivan, additional, McGuire, Andrew T., additional, Yasmeen, Anila, additional, Behrens, Anna-Janina, additional, Ozorowski, Gabriel, additional, van den Kerkhof, Tom L.G.M., additional, Freund, Natalia T., additional, Dosenovic, Pia, additional, Hua, Yuanzi, additional, Gitlin, Alexander D., additional, Cupo, Albert, additional, van der Woude, Patricia, additional, Golabek, Michael, additional, Sliepen, Kwinten, additional, Blane, Tanya, additional, Kootstra, Neeltje, additional, van Breemen, Mariëlle J., additional, Pritchard, Laura K., additional, Stanfield, Robyn L., additional, Crispin, Max, additional, Ward, Andrew B., additional, Stamatatos, Leonidas, additional, Klasse, Per Johan, additional, Moore, John P., additional, Nemazee, David, additional, Nussenzweig, Michel C., additional, Wilson, Ian A., additional, and Sanders, Rogier W., additional

Published
2017
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10. Direct Probing of Germinal Center Responses Reveals Immunological Features and Bottlenecks for Neutralizing Antibody Responses to HIV Env Trimer

Author

Havenar-Daughton, Colin, primary, Carnathan, Diane G., additional, Torrents de la Peña, Alba, additional, Pauthner, Matthias, additional, Briney, Bryan, additional, Reiss, Samantha M., additional, Wood, Jennifer S., additional, Kaushik, Kirti, additional, van Gils, Marit J., additional, Rosales, Sandy L., additional, van der Woude, Patricia, additional, Locci, Michela, additional, Le, Khoa M., additional, de Taeye, Steven W., additional, Sok, Devin, additional, Mohammed, Ata Ur Rasheed, additional, Huang, Jessica, additional, Gumber, Sanjeev, additional, Garcia, AnaPatricia, additional, Kasturi, Sudhir P., additional, Pulendran, Bali, additional, Moore, John P., additional, Ahmed, Rafi, additional, Seumois, Grégory, additional, Burton, Dennis R., additional, Sanders, Rogier W., additional, Silvestri, Guido, additional, and Crotty, Shane, additional

Published
2016
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11. Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo

Author

Medina-Ramírez, Max, Garces, Fernando, Escolano, Amelia, Skog, Patrick, de Taeye, Steven W., Del Moral-Sanchez, Ivan, McGuire, Andrew T., Yasmeen, Anila, Behrens, Anna-Janina, Ozorowski, Gabriel, van den Kerkhof, Tom L.G.M., Freund, Natalia T., Dosenovic, Pia, Hua, Yuanzi, Gitlin, Alexander D., Cupo, Albert, van der Woude, Patricia, Golabek, Michael, Sliepen, Kwinten, Blane, Tanya, Kootstra, Neeltje, van Breemen, Mariëlle J., Pritchard, Laura K., Stanfield, Robyn L., Crispin, Max, Ward, Andrew B., Stamatatos, Leonidas, Klasse, Per Johan, Moore, John P., Nemazee, David, Nussenzweig, Michel C., Wilson, Ian A., Sanders, Rogier W., AII - Infectious diseases, Medical Microbiology and Infection Prevention, Other departments, Experimental Immunology, APH - Aging & Later Life, and AII - Amsterdam institute for Infection and Immunity

Subjects
Crystallography, X-Ray, Antibodies, Neutralizing, Article, HIV Envelope Protein gp160, Protein Structure, Tertiary, Mice, HEK293 Cells, HIV-1, Animals, Humans, Gene Knock-In Techniques, Protein Multimerization, Research Articles
Abstract

Induction of broadly neutralizing antibodies (bNAbs) to HIV would be a major advance toward an effective vaccine. A critical step in this process is the activation of naive B cells expressing bNAb precursors. Medina-Ramírez et al. developed a BG505 SOSIP.v4.1-GT1 trimer that activates bNAb precursors in vitro and in vivo., Induction of broadly neutralizing antibodies (bNAbs) by HIV-1 envelope glycoprotein immunogens would be a major advance toward an effective vaccine. A critical step in this process is the activation of naive B cells expressing germline (gl) antibody precursors that have the potential to evolve into bNAbs. Here, we reengineered the BG505 SOSIP.664 glycoprotein to engage gl precursors of bNAbs that target either the trimer apex or the CD4-binding site. The resulting BG505 SOSIP.v4.1-GT1 trimer binds multiple bNAb gl precursors in vitro. Immunization experiments in knock-in mice expressing gl-VRC01 or gl-PGT121 show that this trimer activates B cells in vivo, resulting in the secretion of specific antibodies into the sera. A crystal structure of the gl-targeting trimer at 3.2-Å resolution in complex with neutralizing antibodies 35O22 and 9H+109L reveals a native-like conformation and the successful incorporation of design features associated with binding of multiple gl-bNAb precursors.

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12. Stabilization of the gp120 V3 loop through hydrophobic interactions reduces the immunodominant V3-directed non-neutralizing response to HIV-1 envelope trimers.

Author

de Taeye SW, de la Peña AT, Vecchione A, Scutigliani E, Sliepen K, Burger JA, van der Woude P, Schorcht A, Schermer EE, van Gils MJ, LaBranche CC, Montefiori DC, Wilson IA, Moore JP, Ward AB, and Sanders RW

Subjects
Antibodies, Neutralizing immunology, Epitopes immunology, HEK293 Cells, HIV Antibodies immunology, HIV Envelope Protein gp120 immunology, Humans, Hydrophobic and Hydrophilic Interactions, Protein Stability, Antibodies, Neutralizing chemistry, Epitopes chemistry, HIV Antibodies chemistry, HIV Envelope Protein gp120 chemistry, Protein Multimerization
Abstract

To provide protective immunity against circulating primary HIV-1 strains, a vaccine most likely has to induce broadly neutralizing antibodies to the HIV-1 envelope glycoprotein (Env) spike. Recombinant Env trimers such as the prototype BG505 SOSIP.664 that closely mimic the native Env spike can induce autologous neutralizing antibodies (NAbs) against relatively resistant (tier 2) primary viruses. Ideally, Env immunogens should present broadly neutralizing antibody epitopes but limit the presentation of immunodominant non-NAb epitopes that might induce off-target and potentially interfering responses. The V3 loop in gp120 is such a non-NAb epitope that can effectively elicit non-NAbs when animals are immunized with SOSIP.664 trimers. V3 immunogenicity can be diminished, but not abolished, by reducing the conformational flexibility of trimers via targeted sequence changes, including an A316W substitution in V3, that create the SOSIP.v4.1 and SOSIP.v5.2 variants. Here, we further modified these trimer designs by introducing leucine residues at V3 positions 306 and 308 to create hydrophobic interactions with the tryptophan residue at position 316 and with other topologically proximal sites in the V1V2 domain. Together, these modifications further stabilized the resulting SOSIP.v5.2 S306L/R308L trimers in the prefusion state in which V3 is sequestered. When we tested these trimers as immunogens in rabbits, the induction of V3 non-NAbs was significantly reduced compared with the SOSIP.v5.2 trimers and even more so compared with the SOSIP.664 prototype, without affecting the autologous NAb response. Hence, these additional trimer sequence modifications may be beneficial for immunization strategies that seek to minimize off-target non-NAb responses., (© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.)

Published
2018
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