Search

Your search keyword '"veronique cadoret"' showing total 36 results
Start Over Author "veronique cadoret"
36 results on '"veronique cadoret"'

1. Genomic assessment of human cumulus cell marker genes as predictors of oocyte developmental competence: impact of various experimental factors.

Author

Prisca Feuerstein, Vincent Puard, Catherine Chevalier, Raluca Teusan, Veronique Cadoret, Fabrice Guerif, Remi Houlgatte, and Dominique Royere

Subjects
Medicine, Science
Abstract

Single embryo transfer (SET) is the most successful way to reduce the frequency of multiple pregnancies following in vitro fertilisation. However, selecting the embryo for SET with the highest chances of pregnancy remains a difficult challenge since morphological and kinetics criteria provide poor prediction of both developmental and implantation ability. Partly through the expression of specific genes, the oocyte-cumulus interaction helps the oocyte to acquire its developmental competence. Our aim was therefore to identify at the level of cumulus cells (CCs) genes related to oocyte developmental competence.197 individual CCs were collected from 106 patients undergoing an intra-cytoplasmic sperm injection procedure. Gene expression of CCs was studied using microarray according to the nuclear maturity of the oocyte (immature vs. mature oocyte) and to the developmental competence of the oocyte (ability to reach the blastocyst stage after fertilisation). Microarray study was followed by a meta-analysis of the behaviour of these genes in other datasets available in Gene Expression Omnibus which showed the consistency of this list of genes. Finally, 8 genes were selected according to oocyte developmental competence from the 308 differentially expressed genes (p

Published
2012
Full Text
View/download PDF

2. Regulation of initiation of follicle growth and dynamics of early follicular development in the sheep

Author

Danielle Monniaux, veronique cadoret, Frederique Clement, Stéphane Fabre, Yann Locatelli, Philippe Monget, Rozenn Dalbies-Tran, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Mathematical and Mechanical Modeling with Data Interaction in Simulations for Medicine (M3DISIM), Laboratoire de mécanique des solides (LMS), École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École polytechnique (X)-Inria Saclay - Ile de France, Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École polytechnique (X)-Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Mines Paris - PSL (École nationale supérieure des mines de Paris), Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), and Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Follicle, endocrine system, Oocyte, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Ovary, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
Abstract

International audience; Primordial follicles embedded in the ovarian cortex are the source of developing follicles. Follicle growth activa- tion and development up to the small antral follicle stage are controlled by cell interactions. The sheep ovary offers an appropriate non-rodent model to study these interac- tions, thanks to the development of in vitro and in vivo experimental approaches, ex vivo molecular analyses and in silico mathematical modeling. Each primordial follicle, composed of an oocyte surrounded by a single layer of quiescent granulosa cells, relies on nutrients and growth factors supplied by the surrounding stroma of connective tissue. In vitro cultures of ovarian cortex have shown that primordial follicles are activated by the lifting of mech- anisms maintaining quiescence, some of them involving AMH secreted by already growing follicles. Afterwards, follicle development is supported by a finely tuned molec- ular dialog between the growing oocyte and proliferating granulosa cells. The isolation of preantral follicles and their development in vitro as individual follicles perturb this dialog, leading to an acceleration of follicular matu- ration. In vivo, mutations in the oocyte factors BMP15, GDF9 or their receptor BMPR1B also impair this dia- log, leading to an imbalance between oocyte growth and cell proliferation, which can be reproduced by models for cell dynamics. During the growth of preantral follicles, the recruitment and differentiation of theca cells from the ovarian stroma provide them with a structural and vascularized support. In vivo exposure of sheep fetal ovaries to testosterone imprints the stroma cells so that the expression of genes involved in extracellular matrix organization and cell-cell adhesion is affected in theca at adulthood; this leads to a lower ovarian tissue rigid- ity that can account for the accelerated follicle growth observed in androgenized ewes. A better knowledge of cell interactions during early follicular development will help to improve the biotechnology methods of fertility preservation.

Published
2018

3. Lipid metabolism and female reproduction: role at the ovary level

Author

Virginie Maillard, Sébastien Elis, Sandrine Freret, Valérie Labas, Ana-Paula Teixeira Gomes, veronique cadoret, Philippe Monget, Svetlana Uzbekova, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Plate-Forme d'Analyse Intégrative des Biomarqueurs, Institut National de la Recherche Agronomique (INRA), INRA, Apis-gène, Région Val de Loire, Université de Haute Bretagne ( Rennes 2 ) (UR 2). Rennes, FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

4. Biomarkers of oocyte quality in cumulus cells using Reverse Phase Protein Array (RPPA)

Author

Vincent Puard, Thomas Bourquard, veronique cadoret, Fabrice Guerif, Eric Reiter, Dominique Royère, ProdInra, Migration, Life Sciences, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), George Mason University. USA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
reverse phase protein array, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology
Abstract

International audience

Published
2015

5. Apoptosis, Onset and Maintenance of Spermatogenesis: Evidence for the Involvement of Kit in Kit-Haplodeficient Mice1

Author

Marie Thérèse Hochereau-de Reviers, J. Lansac, Dominique Royere, Fabrice Guerif, Veronique Cadoret, Jean-Jacques Panthier, Florence Bernex, and Valerie Rahal-Perola

Subjects
Reporter gene, medicine.diagnostic_test, Ratón, Stem cell factor, Cell Biology, General Medicine, Biology, Flow cytometry, Andrology, Seminiferous tubule, medicine.anatomical_structure, Reproductive Medicine, Apoptosis, Immunology, medicine, Spermatogenesis, Germ cell
Abstract

Kit/stem cell factor (SCF ) has been reported to be involved in survival and proliferation of male differentiating spermatogonial cells. This kinetics study was designed to assess the role of Kit/SCF during spermatogenesis in mice, and the extent of male programmed germ cell death was measured between 8 and 150 days of age. For this purpose, 129/Sv inbred mice in which one Kit allele was inactivated by an nlslacZ sequence insertion (KitW-lacZ/+) were compared with 129/Sv inbred mice with wild-type alleles at the Kit locus. Four different approaches were used: 1) morphometric study to assess spermatogenesis, 2) flow cytometry to study testicular cell ploidy, 3) in situ end labeling to detect apoptosis, and 4) follow-up of reporter gene expression. Spermatogenesis was lower in KitW-lacZ/+ heterozygous mice both at the onset of spermatogenesis and during adulthood. Indeed, greater apoptosis occurred at the onset of spermatogenesis. This was followed in the adult by a smaller seminiferous tubule diam...

Published
2002

6. Top-down proteomic strategy to identify oocyte quality biomarkers revealed by intact cells MALDI-TOF profiling

Author

Valérie Labas, Audrey Gargaros, Ana Paula Teixeira-Gomes, veronique cadoret, Fabrice Guerif, Dominique Royère, Svetlana Uzbekova, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Hôpital Bretonneau, Assistance Publique - Hôpitaux de Paris (AP-HP), Société Française de Spectrométrie de Masse (SFSM). Orléans, FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], cellule cumulus, spectrométrie de masse, reproduction assistée, ovocyte, biomarqueur, ComputingMilieux_MISCELLANEOUS, Autre (Sciences du Vivant)
Abstract

National audience

Published
2012

7. Profiling MALDI-TOF de cellules entières et identification de biomarqueurs de la qualité ovocytaire par Top Down

Author

Valérie Labas, Audrey Gargaros, Ana Paula Teixeira-Gomes, veronique cadoret, Fabrice Guerif, Dominique Royère, Svetlana Uzbekova, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plate-Forme d'Analyse Intégrative des Biomarqueurs, Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Hôpital Bretonneau, Assistance Publique - Hôpitaux de Paris (AP-HP), Société Française d'Electrophorèse et d'Analyse Protéomique (SFEAP). FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2012

8. Protéomique par Intact Cells Maldi-Tof Mass Spectrometry sur cumulus humain. Une aide à la décision pour le transfert mono-embryonnaire en Assistance Médicale à la Procréation ?

Author

veronique cadoret, Svetlana Uzbekova, Lucie Spina, Vincent Puard, Fabrice Guerif, Dominique Royère, Valérie Labas, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Service de Médecine et Biologie de la Reproduction, Groupement de Moyens Scientifiques et Techniques (GMSTT). Labo/service de l'auteur, Tours, FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2011

9. Treating women under 36 years old without top-quality embryos on day 2: a prospective study comparing double embryo transfer with single blastocyst transfer

Author

Cynthia Frapsauce, Veronique Cadoret, Dominique Royere, C. Chavez, Fabrice Guerif, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Service de Médecine et Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, medicine.medical_specialty, Time Factors, Pregnancy Rate, Cleavage Stage, Ovum, [SDV]Life Sciences [q-bio], Fertilization in Vitro, transfert de blastocyste, Biology, Cryopreservation, 03 medical and health sciences, 0302 clinical medicine, blastocyste, Pregnancy, medicine, Humans, Prospective Studies, Blastocyst, Prospective cohort study, reproduction humaine, 030304 developmental biology, Gynecology, 0303 health sciences, 030219 obstetrics & reproductive medicine, cleavage stage embryo, Rehabilitation, Blastocyst Transfer, Age Factors, Pregnancy Outcome, Obstetrics and Gynecology, multiple deliveries, Embryo culture, Embryo Transfer, Embryo transfer, Pregnancy rate, Treatment Outcome, medicine.anatomical_structure, Reproductive Medicine, embryonic structures, Gestation, Female, delivery
Abstract

BACKGROUND Embryologists currently face a challenge when counselling patients regarding the stage and the number of embryos to transfer when no top-quality embryos (TQE) are available. METHODS The aim of this study was to evaluate the efficacy of single blastocyst transfer (SBT) in comparison with the transfer of two cleavage-stage embryos in women under 36 years old. A total of 450 women under 36 years undergoing their first or second IVF treatment who had no TQE on Day 2 were included in this prospective study. Couples were assigned to either a SBT or a double cleavage-stage embryo transfer (DET). The clinical end-points monitored were rates of implantation, delivery and multiple deliveries. RESULTS The rate of transfer was significantly lower for couples assigned to the SBT group compared with the DET group (88 versus 100%, respectively, P < 0.001) while the delivery rate per oocyte retrieval was similar in both groups (26.7%). By contrast, the rate of multiple deliveries was significantly lower in the SBT group compared with the DET group (3.3 versus 23.3%, respectively, P < 0.01). Blastocyst cryopreservation was twice as high in the SBT group compared with the DET group (39 versus 18%, respectively, P < 0.001). CONCLUSIONS These findings show the value of extended embryo culture for couples without TQE. In such situations, delaying embryo transfer in order to select a single blastocyst with the highest potential for implantation can reduce the number of multiple pregnancies. Furthermore, our results demonstrate that extended culture allows blastocyst cryopreservation from embryos not available for Day 2 cryopreservation.

Published
2011

10. Does early morphology provide additional selection power to blastocyst selection for transfer?

Author

Dominique Royere, C. Chavez, Veronique Cadoret, R. Bidault, Malak Lemseffer, O. Gasnier, M.H. Saussereau, Fabrice Guerif, J. Leger, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Service de Médecine et Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Université Francois Rabelais [Tours], CIC 202, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)

Subjects
medicine.medical_specialty, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Growth kinetics, kinetic, Embryonic Development, Biology, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, medicine, Humans, implantation, Blastocyst, Embryo Implantation, Prospective Studies, Birth Rate, Selection (genetic algorithm), reproductive and urinary physiology, 030304 developmental biology, 0303 health sciences, 030219 obstetrics & reproductive medicine, Obstetrics, urogenital system, Embryogenesis, Obstetrics and Gynecology, Embryo, blastocyst score, Embryo Transfer, Predictive value, Embryo transfer, live birth rate, medicine.anatomical_structure, Reproductive Medicine, single blastocyst transfer, embryonic structures, embryo score, Female, Live birth, Developmental Biology
Abstract

Routine early developmental parameters are widely used in IVF centres to evaluate embryo development and fresh single-blastocyst transfer currently seems superior to single-embryo transfer. Would early morphological parameters help to choose the single blastocyst to be transferred, thereby improving the chances of implantation and live birth rate? This prospective observational study analysed the individual outcomes of 2617 embryos from 511 IVF couples scheduled for a single-blastocyst transfer. Embryo and blastocyst scores were constructed. There was a clear relationship between the kinetics and morphology of blastocysts and further implantation and live birth rate. There was a limited predictive value of embryo score with regard to blastocyst development and growth kinetics. Implanted and non-implanted blastocysts showed similar embryo scores. Thus usual morphological parameters on days 1 and 2 seem to have no additional value in indicating the right blastocyst to transfer. Non-invasive approaches might be helpful to increase the chances of implantation in the future. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Published
2010

11. ICM-MS profiling of cumulus cells as a non-invasive method to access potential markers of oocyte maturation in bovine, equine and human

Author

Valérie Labas, Nadine Gérard, veronique cadoret, Lucie Spina, Dominique Royère, Svetlana Uzbekova, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], ICM-MS, TECHNOLOGIE DE LA REPRODUCTION ASSISTEE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2009

12. Progress and challenges in developing organoids in farm animal species for the study of reproduction and their applications to reproductive biotechnologies

Author

Guillaume Bourdon, Véronique Cadoret, Gilles Charpigny, Anne Couturier-Tarrade, Rozenn Dalbies-Tran, Maria-José Flores, Pascal Froment, Mariam Raliou, Karine Reynaud, Marie Saint-Dizier, and Alice Jouneau

Subjects
Embryoid, Endometrium, Follicle, Organoid, Ovary, Oviduct, Veterinary medicine, SF600-1100
Abstract

Abstract Within the past decades, major progress has been accomplished in isolating germ/stem/pluripotent cells, in refining culture medium and conditions and in establishing 3-dimensional culture systems, towards developing organoids for organs involved in reproduction in mice and to some extent in humans. Haploid male germ cells were generated in vitro from primordial germ cells. So were oocytes, with additional support from ovarian cells and subsequent follicle culture. Going on with the female reproductive tract, spherical oviduct organoids were obtained from adult stem/progenitor cells. Multicellular endometrial structures mimicking functional uterine glands were derived from endometrial cells. Trophoblastic stem cells were induced to form 3-dimensional syncytial-like structures and exhibited invasive properties, a crucial point for placentation. Finally, considering the embryo itself, pluripotent embryonic cells together with additional extra-embryonic cells, could self-organize into a blastoid, and eventually into a post-implantation-like embryo. Most of these accomplishments have yet to be reached in farm animals, but much effort is devoted towards this goal. Here, we review the progress and discuss the specific challenges of developing organoids for the study of reproductive biology in these species. We consider the use of such organoids in basic research to delineate the physiological mechanisms involved at each step of the reproductive process, or to understand how they are altered by environmental factors relevant to animal breeding. We evaluate their potential in reproduction of animals with a high genetic value, from a breeding point of view or in the context of preserving local breeds with limited headcounts.

Published
2021
Full Text
View/download PDF

13. Etude de l’expression de gènes dans les cellules de cumulus humaines : une approche de la compétence ovocytaire

Author

Prisca Feuerstein, veronique cadoret, Rozenn Dalbies-Tran, Fabrice Guerif, Dominique Royère, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
[SDV] Life Sciences [q-bio], BIOLOGIE DE LA REPRODUCTION, [SDV]Life Sciences [q-bio], COMPETENCE OVOCYTAIRE, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

14. Oocyte–cumulus dialog: a real time PCR and protein approach

Author

veronique cadoret, Rozenn Dalbies-Tran, Fabrice Guerif, Dominique Royère, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, ADN MESSAGER
Abstract

International audience

Published
2005

15. Differentiation factors of human cumulus cells and oocyte quality

Author

Langlois, M. L., Fabrice Guerif, veronique cadoret, Dominique Royère, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, ADN MESSAGER
Abstract

International audience

Published
2004

16. Kit, spermatogenesis and fertility: experimental model in transgenic mice

Author

veronique cadoret, Fabrice Guerif, Hochereau Reviers, M. T., Jean-Jacques Panthier, Dominique Royère, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Génétique Moléculaire et Cellulaire (UGMC), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], SOURIS TRANSGENIQUE, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2002

17. Apoptosis, onset and maintenance of spermatogenesis: evidence for the involvement of Kit in Kit-haplodeficient mice

Author

Fabrice, Guerif, Veronique, Cadoret, Valerie, Rahal-Perola, Jacques, Lansac, Florence, Bernex, Jean Jacques, Panthier, Marie Thérèse, Hochereau-de Reviers, and Dominique, Royere

Subjects
Male, Mice, Knockout, Ploidies, Tissue Fixation, Apoptosis, Mice, Inbred Strains, Haploidy, Seminiferous Tubules, Flow Cytometry, beta-Galactosidase, Mice, Proto-Oncogene Proteins c-kit, Fertility, Gene Expression Regulation, Lac Operon, Genes, Reporter, Pregnancy, Testis, Androgens, In Situ Nick-End Labeling, Animals, Female, Spermatogenesis, Alleles
Abstract

Kit/stem cell factor (SCF ) has been reported to be involved in survival and proliferation of male differentiating spermatogonial cells. This kinetics study was designed to assess the role of Kit/SCF during spermatogenesis in mice, and the extent of male programmed germ cell death was measured between 8 and 150 days of age. For this purpose, 129/Sv inbred mice in which one Kit allele was inactivated by an nlslacZ sequence insertion (Kit(W-lacZ/+)) were compared with 129/Sv inbred mice with wild-type alleles at the Kit locus. Four different approaches were used: 1) morphometric study to assess spermatogenesis, 2) flow cytometry to study testicular cell ploidy, 3) in situ end labeling to detect apoptosis, and 4) follow-up of reporter gene expression. Spermatogenesis was lower in Kit(W-lacZ/+) heterozygous mice both at the onset of spermatogenesis and during adulthood. Indeed, greater apoptosis occurred at the onset of spermatogenesis. This was followed in the adult by a smaller seminiferous tubule diameter and a lower ratio between type B spermatogonia and type A stem spermatogonia in Kit(W-lacZ/+) mice compared with Kit(+/+) mice. These differences are probably related to the Kit haplodeficiency, which was the only difference between the two genotypes. Germ cell counts and testicular cell ploidy revealed delayed meiosis in Kit(W-lacZ/+) mice. Reporter gene expression confirmed expression of the Kit gene at the spermatogonial stage and also revealed Kit expression during the late pachytene/diplotene transition. These results suggest involvement of Kit/SCF at different stages of spermatogenesis.

Published
2002

18. C-kit et spermatogenèse : approches in situ et cyto-fluorimétriques chez la souris transgénique Wlacz/+

Author

Fabrice Guerif, veronique cadoret, Reviers, M. T., Levern, Y., Dominique Kerboeuf, Dominique Royère, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Station de Pathologie aviaire et parasitologie [Nouzilly] (PAP), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2001

19. Le processus d'apoptose et le contrôle des populations cellulaires dans la gonade mâle : implication du proto-oncogène C-KIT

Author

veronique cadoret, Fabrice Guerif, Hochereau Reviers, M. T., Panthier, J. J., Lansac, J., Dominique Royère, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], C-KIT, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2001

20. Expression of kit receptor during spermatogenesis: use of a transgenic heterozygous mice Wlacz/+

Author

veronique cadoret, Fabrice Guerif, Hochereau Reviers, M. T., Jean-Jacques Panthier, Lansac, J., Dominique Royère, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Génétique Moléculaire et Cellulaire (UGMC), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], GAMETOGENESE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], SOURIS TRANSGENIQUE, GENE C-KIT, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2000

21. Testis expression of kit receptor through a transgenic heterozygote mice Wlacz/+

Author

veronique cadoret, Fabrice Guerif, Hochereau Reviers, M. T., Jean-Jacques Panthier, Lansac, J., Dominique Royère, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Génétique Moléculaire et Cellulaire (UGMC), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], GENE C-KIT, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2000

22. Genomic Assessment of Human Cumulus Cell Marker Genes as Predictors of Oocyte Developmental Competence: Impact of Various Experimental Factors

Author

Dominique Royere, Vincent Puard, Fabrice Guerif, Raluca Teusan, Prisca Feuerstein, Catherine Chevalier, Rémi Houlgatte, Veronique Cadoret, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Thérapeutique, Université de Nantes (UN), PRES Université Nantes Angers Le Mans (UNAM), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Royère, Dominique, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
Embryology, Microarray, medicine.medical_treatment, lcsh:Medicine, Cell Communication, Transcriptomes, Transcriptome, 0302 clinical medicine, Pregnancy, Genome Databases, Pathology, Single Embryo Transfer, lcsh:Science, Oligonucleotide Array Sequence Analysis, Genetics, Regulation of gene expression, 0303 health sciences, Cumulus Cells, 030219 obstetrics & reproductive medicine, Multidisciplinary, biology, Obstetrics and Gynecology, Cell Differentiation, évaluation génomique, Genomics, medicine.anatomical_structure, Medicine, Female, DNA microarray, Autre (Sciences du Vivant), Research Article, Adult, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Perilipin 2, pcr quantitative, Sequence Databases, Perilipin-2, reproduction, Andrology, 03 medical and health sciences, Genome Analysis Tools, Diagnostic Medicine, medicine, Humans, Sperm Injections, Intracytoplasmic, Biology, 030304 developmental biology, In vitro fertilisation, Genome, Human, Gene Expression Profiling, cellule du cumulus, extraction adn, lcsh:R, marqueur cellulaire, Membrane Proteins, Ribonuclease, Pancreatic, Female Subfertility, Oocyte, Gene expression profiling, Gene Expression Regulation, Oocytes, biology.protein, lcsh:Q, icsi, humain, Genome Expression Analysis, Biomarkers, RGS Proteins, Developmental Biology, General Pathology
Abstract

Background Single embryo transfer (SET) is the most successful way to reduce the frequency of multiple pregnancies following in vitro fertilisation. However, selecting the embryo for SET with the highest chances of pregnancy remains a difficult challenge since morphological and kinetics criteria provide poor prediction of both developmental and implantation ability. Partly through the expression of specific genes, the oocyte-cumulus interaction helps the oocyte to acquire its developmental competence. Our aim was therefore to identify at the level of cumulus cells (CCs) genes related to oocyte developmental competence. Methodology/Principal Findings 197 individual CCs were collected from 106 patients undergoing an intra-cytoplasmic sperm injection procedure. Gene expression of CCs was studied using microarray according to the nuclear maturity of the oocyte (immature vs. mature oocyte) and to the developmental competence of the oocyte (ability to reach the blastocyst stage after fertilisation). Microarray study was followed by a meta-analysis of the behaviour of these genes in other datasets available in Gene Expression Omnibus which showed the consistency of this list of genes. Finally, 8 genes were selected according to oocyte developmental competence from the 308 differentially expressed genes (p

Published
2012

23. Heterologous and homologous desensitizations of the plasminogen activator response of rat Sertoli cells by FSH and isoproterenol

Author

veronique cadoret, Florian Jean Louis Guillou, Yves Combarnous, ProdInra, Migration, Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, RAT, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1994

24. Binding specificity and stimulation efficiency of gonadotropins

Author

Yves Combarnous, Apparailly, F., François Lecompte, Chopineau, M., Fontaine, I., Florian Jean Louis Guillou, Lagarde, D., veronique cadoret, Thierry Magallon, Nadine Martinat, Maurel, M. C., Dominique Royère, Sabine Suire, ProdInra, Migration, Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], BIOCHIMIE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], HORMONE GONADOTROPE, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
1993

25. Bisphenol S Impairs Oestradiol Secretion during In Vitro Basal Folliculogenesis in a Mono-Ovulatory Species Model

Author

Claire Vignault, Véronique Cadoret, Peggy Jarrier-Gaillard, Pascal Papillier, Ophélie Téteau, Alice Desmarchais, Svetlana Uzbekova, Aurélien Binet, Fabrice Guérif, Sebastien Elis, and Virginie Maillard

Subjects
ovary, endocrine disruptors, follicular growth, hormonal secretions, gene expression, bisphenols, Chemical technology, TP1-1185
Abstract

Bisphenol S (BPS) affects terminal folliculogenesis by impairing steroidogenesis in granulosa cells from different species. Nevertheless, limited data are available on its effects during basal folliculogenesis. In this study, we evaluate in vitro the effects of a long-term BPS exposure on a model of basal follicular development in a mono-ovulatory species. We cultured ovine preantral follicles (180–240 μm, n = 168) with BPS (0.1 μM (possible human exposure dose) or 10 μM (high dose)) and monitored antrum appearance and follicular survival and growth for 15 days. We measured hormonal secretions (oestradiol (at day 13 [D13]), progesterone and anti-Müllerian hormone [D15]) and expression of key follicular development and redox status genes (D15) in medium and whole follicles, respectively. BPS (0.1 µM) decreased oestradiol secretion compared with the control (−48.8%, p < 0.001), without significantly impairing antrum appearance, follicular survival and growth, anti-Müllerian hormone and progesterone secretion and target gene expression. Thus, BPS could also impair oestradiol secretion during basal folliculogenesis as it is the case during terminal folliculogenesis. It questions the use of BPS as a safe BPA substitute in the human environment. More studies are required to elucidate mechanisms of action of BPS and its effects throughout basal follicular development.

Published
2022
Full Text
View/download PDF

26. A Comparative Analysis of Oocyte Development in Mammals

Author

Rozenn Dalbies-Tran, Véronique Cadoret, Alice Desmarchais, Sébastien Elis, Virginie Maillard, Philippe Monget, Danielle Monniaux, Karine Reynaud, Marie Saint-Dizier, and Svetlana Uzbekova

Subjects
oocyte, mammals, evolution, gene expression, posttranscriptional control, Gdf9, Cytology, QH573-671
Abstract

Sexual reproduction requires the fertilization of a female gamete after it has undergone optimal development. Various aspects of oocyte development and many molecular actors in this process are shared among mammals, but phylogeny and experimental data reveal species specificities. In this chapter, we will present these common and distinctive features with a focus on three points: the shaping of the oocyte transcriptome from evolutionarily conserved and rapidly evolving genes, the control of folliculogenesis and ovulation rate by oocyte-secreted Growth and Differentiation Factor 9 and Bone Morphogenetic Protein 15, and the importance of lipid metabolism.

Published
2020
Full Text
View/download PDF

27. Non invasive assessment of embryo quality: Proteomics, metabolomics and oocyte-cumulus dialogue

Author

Dominique Royère, Prisca Feuerstein, veronique cadoret, Vincent Puard, Svetlana Uzbekova, Rozenn Dalbies-Tran, Teusan, R., Houlgatte, R., Valérie Labas, and Fabrice Guerif

Subjects
Proteomics, Cumulus Cells, Fetus, Reproductive Techniques, Assisted, Pregnancy, Oocytes, Animals, Embryonic Development, Humans, Metabolomics, Female
Abstract

Preimplantation embryo development is one of the key features with implantation itself to achieve a pregnancy. Assisted Reproductive Technologies both in human and animal have improved our knowledge on these events, although it remains elusive to predict embryo potential to give a baby. Among various ways to define embryo viability, noninvasive approaches get a serious advantage linked to the final transfer of the embryo. Techniques devoted to characterize the embryo secretome using proteomic or metabolomic approaches may be non invasive. Based on a direct identification of products of the embryo metabolism or an assessment of profile(s) related with embryo viability, they have greatly improved their sensitivity to allow their use in clinical embryology, once validated. Oocyte-cumulus dialogue, as a key factor for oocyte competence to meiosis and embryo development, was particularly concerned with both genomic and proteomic assessment of cumulus cells. While it is not possible to designate at the time being which among these approaches will be robust and cost-efficient enough to help routinely the clinical embryologist in assisted reproductive techniques (ART), one can predict that our ability to select the "right" embryo will combine morphological criteria already available with validated biomarkers.

28. Recherche de biomarqueurs de la maturation ovocytaire par spectrométrie de masse sur cellules folliculaires entières : apport d'une analyse comparative multi-espèces

Author

veronique cadoret, Svetlana Uzbekova, Nadine Gérard, Lucie Spina, Dominique Royère, Valérie Labas, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

29. Expression du récepteur c-Kit au cours de la mise en place de la spermatogenèse

Author

veronique cadoret, Fabrice Guerif, Hochereau Reviers, M. T., Jean-Jacques Panthier, Lansac, J., Dominique Royère, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Génétique Moléculaire et Cellulaire (UGMC), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], TRANSGENE lacZ, [INFO]Computer Science [cs], [INFO] Computer Science [cs], GENE C-KIT, ComputingMilieux_MISCELLANEOUS, RECEPTEUR C-KIT
Abstract

National audience

30. Parameters guiding selection of best embryos for transfer after cryopreservation : a reappraisal

Author

Fabrice Guerif, Dominique Royere, Veronique Cadoret, Marie-Laure Couet, Jacques Lansac, Rachel Bidault, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Adult, Male, Blastomeres, animal structures, Pregnancy Rate, Cell Survival, [SDV]Life Sciences [q-bio], Mitosis, Biology, [INFO] Computer Science [cs], Cryopreservation, Andrology, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, Odds Ratio, medicine, Humans, [INFO]Computer Science [cs], Embryo Implantation, Fragmentation (cell biology), ComputingMilieux_MISCELLANEOUS, Retrospective Studies, 030304 developmental biology, 0303 health sciences, 030219 obstetrics & reproductive medicine, Rehabilitation, Embryogenesis, Obstetrics and Gynecology, Embryo, TRANSFERT, Blastomere, Anatomy, Embryo Transfer, Embryo, Mammalian, medicine.disease, Embryo transfer, [SDV] Life Sciences [q-bio], Pregnancy rate, Reproductive Medicine, Multivariate Analysis, embryonic structures, Female
Abstract

BACKGROUND: The purpose of this study was to evaluate the respective influences of blastomere survival and resumption of mitosis on the outcome of frozen–thawed embryos. METHODS: A retrospective analysis was performed in our centre on 363 thawing cycles, involving 4-cell day 2 grade 1 embryos with

31. Désensibilisation par les gonadotropines de leurs cellules-cibles

Author

Florian Jean Louis Guillou, Carine Troispoux, veronique cadoret, ProdInra, Migration, Y. Combarnous (Editeur), P. Volland-Nail (Editeur), Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, and Institut National de la Recherche Agronomique (INRA)

Subjects
LH, [SDV] Life Sciences [q-bio], BIOCHIMIE, [SDV]Life Sciences [q-bio], FSH, AMPC, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

32. Oocyte-cumulus dialog

Author

Prisca Feuerstein, veronique cadoret, Rozenn Dalbies-Tran, Fabrice Guerif, and Dominique Royère

Subjects
Meiosis, Epidermal Growth Factor, Ovarian Follicle, Oocytes, Gap Junctions, Gene Expression, Growth Differentiation Factor 9, Humans, Intercellular Signaling Peptides and Proteins, Female, Cell Communication, Bone Morphogenetic Protein 15, Gonadotropins
Abstract

The dialog between oocyte and cumulus cells brings a major contribution for oocyte meiotic and developmental competence. On the one hand, the oocyte will modulate follicle growth through specific gene expression (Figalpha, GDF-9, BMP15) as well as its meiosis (GPR3 et PDE3A). Beyond its action on proliferation, oocyte will control in part the differentiation of cumulus cells with a particular involvement of GDF-9, BMP15 in this late maturation process. On the other hand, somatic cells are the main targets of gonadotropins and will modulate both oocyte growth and maturation. Gap-junctions between oocyte and cumulus cells have a major role in this interaction, since they allow the action of some oocyte specific genes (GDF9) but also the control of its own metabolism and calcium movements. While ovulation will involve gonadotropins action on somatic cells, EGF-like factors recruited at the cumulus level will participate in this process. Finally we may suspect that improving the knowledge on oocyte-cumulus dialog will contribute to better define oocyte competence, while bringing some clues for in vitro maturation.

33. Analyse protéomique du cumulus humain : une approche indirecte de la viabilité de l’embryon

Author

veronique cadoret, Svetlana Uzbekova, Lucie Spina, Vincent Puard, Fabrice Guerif, Dominique Royère, Valérie Labas, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)

Subjects
[SDV] Life Sciences [q-bio], PROCREATION MEDICALE ASSISTEE, [SDV]Life Sciences [q-bio], REPRODUCTION HUMAINE, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

34. Marqueurs protéiques de la qualité de l'ovocyte identifiés dans les cellules de cumulus par la spectrométrie de masse MALDI-TOF (ICM-MS). Une aide à la décision pour le transfert mono-embryonnaire en Assistance Médicale à la Procréation ?

Author

veronique cadoret, Svetlana Uzbekova, Lucie Spina, Vincent Puard, Fabrice Guerif, Dominique Royère, Valérie Labas, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Service de Médecine et Biologie de la Reproduction, Ecole Nationale Vétérinaire (ENV). Labo/service de l'auteur, Maisons Alfort, FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

35. Involvement of cyclic adenosine monophosphate-dependent protein kinase isozymes in tissue plasminogen activator secretion by rat Sertolli cells simulated with follicle-stimulating hormone in vitro

Author

veronique cadoret, Fabienne Guillon, Yves Combarnous, Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], FSH, RAT, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

36. Semi-quantitative measurement of specific proteins in human cumulus cells using reverse phase protein array

Author

Vincent Puard, Dominique Royere, Veronique Cadoret, Thibaud Tranchant, Christophe Gauthier, Eric Reiter, Fabrice Guerif, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Médecine et Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Merck Serono Grant for Fertility Innovation Award, INRA/Merck Serono fellowship, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], approche transcriptomique, Protein Array Analysis, Computational biology, approche protéomique, Biology, Sensitivity and Specificity, reproduction, Transcriptome, reverse phase protein array, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, biomarker, cumulus cell, oocyte developmental competence, medicine, Humans, compétence ovocytaire, procréation médicalement assistée, 030304 developmental biology, 0303 health sciences, Gene knockdown, Cumulus Cells, 030219 obstetrics & reproductive medicine, HEK 293 cells, fungi, Methodology, Obstetrics and Gynecology, Reverse phase protein lysate microarray, Oocyte, Molecular biology, Blot, HEK293 Cells, medicine.anatomical_structure, cellule somatique, Reproductive Medicine, Oocytes, humain, Biomarkers, Autre (Sciences du Vivant), Proto-oncogene tyrosine-protein kinase Src, Developmental Biology
Abstract

Background The ability to predict the developmental and implantation ability of embryos remains a major goal in human assisted-reproductive technology (ART) and most ART laboratories use morphological criteria to evaluate the oocyte competence despite the poor predictive value of this analysis. Transcriptomic and proteomic approaches on somatic cells surrounding the oocyte (granulosa cells, cumulus cells [CCs]) have been proposed for the identification of biomarkers of oocyte competence. We propose to use a Reverse Phase Protein Array (RPPA) approach to investigate new potential biomarkers of oocyte competence in human CCs at the protein level, an approach that is already used in cancer research to identify biomarkers in clinical diagnostics. Methods Antibodies targeting proteins of interest were validated for their utilisation in RPPA by measuring siRNA-mediated knockdown efficiency in HEK293 cells in parallel with Western blotting (WB) and RPPA from the same lysates. The proteins of interests were measured by RPPA across 13 individual human CCs from four patients undergoing intracytoplasmic sperm injection procedure. Results The knockdown efficiency of VCL, RGS2 and SRC were measured in HEK293 cells by WB and by RPPA and were acceptable for VCL and SRC proteins. The antibodies targeting these proteins were used for their detection in human CCs by RPPA. The detection of protein VCL, SRC and ERK2 (by using an antibody already validated for RPPA) was then carried out on individual CCs and signals were detected for each individual sample. After normalisation by VCL, we showed that the level of expression of ERK2 was almost the same across the 13 individual CCs while the level of expression of SRC was different between the 13 individual CCs of the four patients and between the CCs from one individual patient. Conclusions The exquisite sensitivity of RPPA allowed detection of specific proteins in individual CCs. Although the validation of antibodies for RPPA is labour intensive, RRPA is a sensitive and quantitative technique allowing the detection of specific proteins from very small quantities of biological samples. RPPA may be of great interest in clinical diagnostics to predict the oocyte competence prior to transfer of the embryo using robust protein biomarkers expressed by CCs.

Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results