336 results on '"whole-genome"'
Search Results
2. Association of disease severity and genetic variation during primary Respiratory Syncytial Virus infections.
- Author
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Bender, William, Zhang, Yun, Corbett, Anthony, Chu, Chinyi, Grier, Alexander, Wang, Lu, Qiu, Xing, McCall, Matthew N., Topham, David J., Walsh, Edward E., Mariani, Thomas J., Scheuermann, Richard, Caserta, Mary T., and Anderson, Christopher S.
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RESPIRATORY syncytial virus infections , *GENETIC variation , *GENETIC disorders , *WHOLE genome sequencing , *PESTE des petits ruminants , *HUMAN metapneumovirus infection , *RESPIRATORY syncytial virus - Abstract
Background: Respiratory Syncytial Virus (RSV) disease in young children ranges from mild cold symptoms to severe symptoms that require hospitalization and sometimes result in death. Studies have shown a statistical association between RSV subtype or phylogenic lineage and RSV disease severity, although these results have been inconsistent. Associations between variation within RSV gene coding regions or residues and RSV disease severity has been largely unexplored. Methods: Nasal swabs from children (< 8 months-old) infected with RSV in Rochester, NY between 1977–1998 clinically presenting with either mild or severe disease during their first cold-season were used. Whole-genome RSV sequences were obtained using overlapping PCR and next-generation sequencing. Both whole-genome phylogenetic and non-phylogenetic statistical approaches were performed to associate RSV genotype with disease severity. Results: The RSVB subtype was statistically associated with disease severity. A significant association between phylogenetic clustering of mild/severe traits and disease severity was also found. GA1 clade sequences were associated with severe disease while GB1 was significantly associated with mild disease. Both G and M2-2 gene variation was significantly associated with disease severity. We identified 16 residues in the G gene and 3 in the M2-2 RSV gene associated with disease severity. Conclusion: These results suggest that phylogenetic lineage and the genetic variability in G or M2-2 genes of RSV may contribute to disease severity in young children undergoing their first infection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Genome-wide analysis of the harbour porpoise (Phocoena phocoena) indicates isolation-by-distance across the North Atlantic and potential local adaptation in adjacent waters.
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Autenrieth, Marijke, Havenstein, Katja, De Cahsan, Binia, Canitz, Julia, Benke, Harald, Roos, Anna, Pampoulie, Christophe, Sigurðsson, Guðjón Már, Siebert, Ursula, Olsen, Morten Tange, Biard, Vincent, Heide-Jørgensen, Mads Peter, Öztürk, Ayaka Amaha, Öztürk, Bayram, Lawson, John W., and Tiedemann, Ralph
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HARBOR porpoise ,POPULATION differentiation ,PHYLOGENY ,CETACEA ,SINGLE nucleotide polymorphisms ,HYDROGRAPHY - Abstract
The harbour porpoise (Phocoena phocoena), a highly mobile cetacean species of the Northern Hemisphere, inhabits basins that vary broadly in salinity, temperature, and food availability; such variation can drive divergent adaptation among local populations. To shed light on range-wide population structure and local adaptation, we generated ddRAD sequencing data spanning the entire North Atlantic and the Baltic Sea, as well as the Black Sea as an outgroup, and mapped this data to the high-quality draft genome of the species. We identified 11,978 genome-wide SNPs from 150 individuals, which we used for population genetic inferences. Our results support genetic differentiation between North Atlantic and Baltic Sea populations, with Kattegat as a transition zone. Across the North Atlantic the population differentiation is subtle from west to east, congruent with an isolation-by-distance pattern, but indicates a separation of southern North Sea harbour porpoises. We identified genomic outlier regions, i.e., scaffold regions where SNPs with high F
ST across North Atlantic populations co-occur. Together with the draft genome annotation, these regions could point towards candidate genes for differential local adaptation processes among populations. Furthermore, they enable the development of a SNP panel for routine population assignment which will be useful in a conservation and management context. We identified six outlier loci putatively under positive selection, based on the population structure inferred from the complete SNP set. Our study highlights the value of genome resources in conservation and management and provides a crucial additional resource for the study of harbour porpoise evolution and phylogeny. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. Isolation and identification of Wickerhamiella tropicalis from blood culture by MALDI-MS.
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Satomi Takei, Kanae Teramoto, Junya Fujimura, Megumi Fujiwara, Mai Suzuki, Yukiko Fukui, Yuji Sekiguchi, Takaaki Kawakami, Masayoshi Chonan, Mitsuru Wakita, Yuki Horiuchi, Takashi Miida, Toshio Naito, Teruo Kirikae, Tatsuya Tada, and Yoko Tabe
- Subjects
LYMPHOBLASTIC leukemia ,ENVIRONMENTAL sampling ,MASS spectrometry ,AZOLES ,RIBOSOMAL DNA ,TREATMENT effectiveness ,AMINO acids - Abstract
Wickerhamiella is a genus of budding yeast that is mainly isolated from environmental samples, and 40 species have been detected. The yeast isolated from human clinical samples usually only contain three species: W. infanticola, W. pararugosa and W. sorbophila. In this study, we isolated W. tropicalis from a blood sample of a six-year-old female with a history of B-cell precursor lymphoblastic leukemia in Japan in 2022. Though the strain was morphologically identified as Candida species by routine microbiological examinations, it was subsequently identified as W. tropicalis by sequencing the internal transcribed spacer (ITS) of ribosomal DNA (rDNA). The isolate had amino acid substitutions in ERG11 and FKS1 associated with azole and echinocandin resistance, respectively, in Candida species and showed intermediate-resistant to fluconazole and micafungin. The patient was successfully treated with micafungin. Furthermore, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) detected three novel peaks that are specific for W. tropicalis, indicating that MALDI-MS analysis is useful for rapid detection of Wickerhamiella species in routine microbiological examinations. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Whole-genome sequence and characterization of a marine red yeast, Rhodosporidium sphaerocarpum GDMCC 60679, featuring the assimilation of ammonia nitrogen.
- Author
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Pan, Chuanhao, Yin, Jiayue, Ma, Bo, Wen, Jing, and Luo, Peng
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WHOLE genome sequencing , *MONASCUS purpureus , *AMMONIA , *GENOME size , *WHITELEG shrimp , *CAROTENOIDS , *GENOMES , *HEMOCYANIN - Abstract
A marine red yeast, Rhodosporidium sphaerocarpum , is generally used for the production of lipids and carotenoids. In a previous study, we demonstrated that a marine-derived R. sphaerocarpum GDMCC 60679 can efficiently remove ammonia nitrogen and exhibit multiple probiotic functions for shrimp, Litopenaeus vannamei. Here, we performed a genome assembly of the strain GDMCC 60679 using a combination of the data from Illumina PE and PacBio CLR reads. The genome has a size of 18.03 Mb and consists of 32 contigs with an N50 length of 1,074,774 bp and GC content of 63 %. The genome was predicted to contain 6092 protein-coding genes, 5962 of which were functionally annotated. Metabolic pathways responsible for the ammonia assimilation and the synthesis of lipids and carotenoids were particularly examined to explore and characterize genes contributing to these functions. Whole-genome sequence and annotation of the strain lays a foundation to reveal the molecular mechanism of its prominent biological functions and will facilitate us to further expand new applications of yeasts in Rhodosporidium. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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6. Genomic and proteomic analysis of Salmonella Enteritidis isolated from a patient with foodborne diarrhea.
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Xu, Benjin, Hou, Zhuru, Liu, Ling, and Wei, Jianhong
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SALMONELLA enteritidis , *SALMONELLA food poisoning , *GENOMICS , *FOODBORNE diseases , *SALMONELLA diseases , *PROTEOMICS , *COMPARATIVE genomics - Abstract
Salmonella is a major cause of foodborne diseases and clinical infections worldwide. This study aimed to investigate the drug resistance, genomic characteristics, and protein expression of foodborne Salmonella in Shanxi Province. We isolated a strain of Salmonella Enteritidis from patient feces and designated it 31A. The drug resistance of 31A against 14 antibiotics was determined using an antimicrobial susceptibility test. Whole-genome sequencing and quantitative proteomic analysis were performed on the 31A strain. Functional annotation of drug resistance genes/proteins and virulence genes/proteins was conducted using various databases, such as VFDB, ARDB, CAZY, COG, KOG, CARD, GO, and KEGG. The focus of this study was understanding the mechanisms related to food poisoning, and the genetic evolution of 31A was analyzed through comparative genomics. The 31A strain belonged to ST11 Salmonella Enteritidis and showed resistance to β-lactam and quinolone antibiotics. The genome of 31A had 70 drug resistance genes, 321 virulence genes, 12 SPIs, and 3 plasmid replicons. Functional annotation of these drug resistance and virulence genes revealed that drug resistance genes were mainly involved in defense mechanisms to confer resistance to antibiotics, while virulence genes were mainly associated with cellular motility. There were extensive interactions among the virulence genes, which included SPI-1, SPI-2, flagella, fimbriae, capsules and so on. The 31A strain had a close relationship with ASM2413794v1 and ASM130523v1, which were also ST11 Salmonella Enteritidis strains from Asia and originated from clinical patients, animals, and food. These results suggested minimal genomic differences among strains from different sources and the potential for interhost transmission. Differential analysis of the virulence and drug resistance-related proteins revealed their involvement in pathways related to human diseases, indicating that these proteins mediated bacterial invasion and infection. The integration of genomic and proteomic information led to the discovery that Salmonella can survive in a strong acid environment through various acid resistance mechanisms after entering the intestine with food and then invade intestinal epithelial cells to exert its effects. In this study, we comprehensively analyzed the drug resistance and virulence characteristics of Salmonella Enteritidis 31A using a combination of genomic and proteomic approaches, focusing on the pathogenic mechanism of Salmonella Enteritidis in food poisoning. We found significant fluctuations in various virulence factors during the survival, invasion, and infection of Salmonella Enteritidis, which collectively contributed to its pathogenicity. These results provide important information for the source tracing, prevention, and treatment of clinical infections caused by Salmonella Enteritidis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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7. 产活性氧假交替单胞菌 GCY全基因组序列分析.
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岳 昊, 吴 硕, 王 竞, 李 泽 龙, and 顾 晨
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REACTIVE oxygen species ,MICROORGANISMS - Abstract
Copyright of Journal of Dalian University of Technology / Dalian Ligong Daxue Xuebao is the property of Journal of Dalian University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
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- View/download PDF
8. Whole genome sequencing and functional analysis of a novel biofilm-eradicating strain Nocardiopsis lucentensis EMB25.
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Goel, Nikky, Zaidi, Saniya, and Khare, Sunil Kumar
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WHOLE genome sequencing , *ACTINOBACTERIA , *FUNCTIONAL analysis , *SEQUENCE analysis , *NUCLEOTIDE sequencing , *BACILLUS subtilis , *STAPHYLOCOCCUS aureus , *GENE clusters - Abstract
The process of biofilm formation is intricate and multifaceted, requiring the individual cells to secrete extracellular polymeric substances (EPS) that subsequently aggregate and adhere to various surfaces. The issue of biofilms is a significant concern for public health due to the increased resistance of microorganisms associated with biofilms to antimicrobial agents. The current study describes the whole genome and corresponding functions of a biofilm inhibiting and eradicating actinobacteria isolate identified as Nocardiopsis lucentensis EMB25. The N. lucentensis EMB25 has 6.5 Mbp genome with 71.62% GC content. The genome analysis by BLAST Ring Image Generator (BRIG) revealed it to be closely related to Nocardiopsis dassonvillei NOCA502F. Interestingly, based on orthologous functional groups reflected by average nucleotide identity (ANI) analysis, it was 81.48% similar to N. arvandica DSM4527. Also, it produces lanthipeptides and linear azole(in)e-containing peptides (LAPs) akin to N. arvandica. The secondary metabolite search revealed the presence of major gene clusters involved in terpene, ectoine, siderophores, Lanthipeptides, RiPP-like, and T1PKS biosynthesis. After 24 h of treatment, the cell-free extract effectively eradicates the pre-existing biofilm of P. aeruginosa PseA. Also, the isolated bacteria exhibited antibacterial activity against MRSA, Staphylococcus aureus and Bacillus subtilis bacteria. Overall, this finding offers valuable insights into the identification of BGCs, which contain enzymes that play a role in the biosynthesis of natural products. Specifically, it sheds light on the functional aspects of these BGCs in relation to N. lucentensis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
9. Unraveling the Genetic Population Structure of Mongolian Indigenous Cattle Breeds Using Whole Genome Sequencing Data
- Author
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Tian, Rugang, Nanaie, Hojjat Asadollahpour, Li, Yuan, Wang, Xiao, Zhao, Meng, Li, Hui, Zhang, Hao, Wu, Jianghong, Ma, Wanshu, Series Editor, Widodo, Eko, editor, Ton, Vu Dinh, editor, Tian, Rugang, editor, Man, Norsida, editor, and Mashudi, Mashudi, editor
- Published
- 2023
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10. The genome of sheep ked (Melophagus ovinus) reveals potential mechanisms underlying reproduction and narrower ecological niches
- Author
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Qingxun Zhang, Qingsong Zhou, Shuyi Han, Ying Li, Ye Wang, and Hongxuan He
- Subjects
Melophagus ovinus ,Pathogen diversity ,Genetic diversity ,Whole-genome ,Gene family evolution ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Melophagus ovinus is considered to be of great veterinary health significance. However, little is known about the information on genetic mechanisms of the specific biological characteristics and novel methods for controlling M. ovinus. Results In total, the de novo genome assembly of M. ovinus was 188.421 Mb in size (330 scaffolds, N50 Length: 10.666 Mb), with a mean GC content of 27.74%. A total of 13,372 protein-coding genes were functionally annotated. Phylogenetic analysis indicated that the diversification of M. ovinus and Glossina fuscipes took place 72.76 Mya within the Late Cretaceous. Gene family expansion and contraction analysis revealed that M. ovinus has 65 rapidly-evolving families (26 expansion and 39 contractions) mainly involved DNA metabolic activity, transposases activity, odorant receptor 59a/67d-like, IMD domain-containing protein, and cuticle protein, etc. The universal and tightly conserved list of milk protein orthologues has been assembled from the genome of M. ovinus. Contractions and losses of sensory receptors and vision-associated Rhodopsin genes were significant in M. ovinus, which indicate that the M. ovinus has narrower ecological niches. Conclusions We sequenced, assembled, and annotated the whole genome sequence of M. ovinus, and launches into the preliminary genetic mechanisms analysis of the adaptive evolution characteristics of M. ovinus. These resources will provide insights to understand the biological underpinnings of this parasite and the disease control strategies.
- Published
- 2023
- Full Text
- View/download PDF
11. Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T
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Lingmin Jiang, Jiyoon Seo, Yuxin Peng, Doeun Jeon, Soon Ju Park, Cha Young Kim, Pyoung Il Kim, Chul Hong Kim, Ju Huck Lee, and Jiyoung Lee
- Subjects
Endophyte ,Saccharibacillus brassicae ,Plant growth-promotion ,Whole-genome ,antiSMASH ,Biotechnology ,TP248.13-248.65 ,Microbiology ,QR1-502 - Abstract
Abstract Endophytes can facilitate the improvement of plant growth and health in agriculturally important crops, yet their genomes and secondary metabolites remain largely unexplored. We previously isolated Saccharibacillus brassicae strain ATSA2T from surface-sterilized seeds of kimchi cabbage and represented a novel species of the genus Saccharibacillus. In this study, we evaluated the plant growth-promoting (PGP) effect of strain ATSA2T in kimchi cabbage, bok choy, and pepper plants grown in soils. We found a significant effect on the shoot and root biomass, and chlorophyll contents following strain ATSA2T treatment. Strain ATSA2T displayed PGP traits such as indole acetic acid (IAA, 62.9 μg/mL) and siderophore production, and phosphate solubilization activity. Furthermore, genome analysis of this strain suggested the presence of gene clusters involved in iron acquisition (fhuABD, afuABC, fbpABC, and fepCDG) and phosphate solubilization (pstABCHS, phoABHLU, and phnCDEP) and other phytohormone biosynthesis genes, including indole-3-acetic acid (trpABCDEFG), in the genome. Interestingly, the secondary metabolites cerecidin, carotenoid, siderophore (staphylobactin), and bacillaene underlying plant growth promotion were found in the whole genome via antiSMASH analysis. Overall, physiological testing and genome analysis data provide comprehensive insights into plant growth-promoting mechanisms, suggesting the relevance of strain ATSA2T in agricultural biotechnology.
- Published
- 2023
- Full Text
- View/download PDF
12. Metagenomic sequencing, molecular characterization, and Bayesian phylogenetics of imported type 2 vaccine-derived poliovirus, Spain, 2021.
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Fernandez-Garcia, Maria Dolores, Faye, Martin, Diez-Fuertes, Francisco, Moreno-Docón, Antonio, Chirlaque-López, Maria Dolores, Faye, Ousmane, and Cabrerizo, Maria
- Subjects
NUCLEOTIDE sequencing ,POLIOVIRUS ,PHYLOGENY ,MARKOV chain Monte Carlo ,WHOLE genome sequencing ,ACUTE flaccid paralysis - Abstract
Introduction: In 2021, a type 2 vaccine-derived poliovirus (VDPV2) was isolated from the stool of a patient with acute flaccid paralysis (AFP) admitted to Spain from Senegal. A virological investigation was conducted to characterize and trace the origin of VDPV2. Methods: We used an unbiased metagenomic approach for the whole-genome sequencing of VDPV2 from the stool (pre-treated with chloroform) and from the poliovirus-positive supernatant. Phylogenetic analyses and molecular epidemiological analyses relying on the Bayesian Markov Chain Monte Carlo methodology were used to determine the geographical origin and estimate the date of the initiating dose of the oral poliovirus vaccine for the imported VDPV2. Results: We obtained a high percentage of viral reads per total reads mapped to the poliovirus genome (69.5% for pre-treated stool and 75.8% for isolate) with a great depth of sequencing coverage (5,931 and 11,581, respectively) and complete genome coverage (100%). The two key attenuating mutations in the Sabin 2 strain had reverted (A481G in the 5'UTR and Ile143Thr in VP1). In addition, the genome had a recombinant structure between type-2 poliovirus and an unidentified non-polio enterovirus-C (NPEV-C) strain with a crossover point in the protease-2A genomic region. VP1 phylogenetic analysis revealed that this strain is closely related to VDPV2 strains circulating in Senegal in 2021. According to Bayesian phylogenetics, the most recent common ancestor of the imported VDPV2 could date back 2.6 years (95% HPD: 1.7-3.7) in Senegal. We suggest that all VDPV2s circulating in 2020-21 in Senegal, Guinea, Gambia, and Mauritania have an ancestral origin in Senegal estimated around 2015. All 50 stool samples from healthy case contacts collected in Spain (n = 25) and Senegal (n = 25) and four wastewater samples collected in Spain were poliovirus negative. Discussion: By using a whole-genome sequencing protocol with unbiased metagenomics from the clinical sample and viral isolate with high sequence coverage, efficiency, and throughput, we confirmed the classification of VDPV as a circulating type. The close genomic linkage with strains from Senegal was consistent with their classification as imported. Given the scarce number of complete genome sequences for NPEV-C in public databases, this protocol could help expand poliovirus and NPEV-C sequencing capacity worldwide. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
13. Identification and characterization of siderophilic biocontrol strain SL-44 combined with whole genome.
- Author
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Xiang, Huichun, He, Yanhui, Wang, Xiaobo, Wang, Jianwen, Li, Tao, Zhu, Shuangxi, Zhang, Ziyan, Xu, Xiaolin, and Wu, Zhansheng
- Subjects
PLANT growth ,PLANT growth-promoting rhizobacteria ,BACILLUS subtilis ,WHOLE genome sequencing ,NITROGEN fixation ,FERTILIZERS ,RHIZOSPHERE - Abstract
Using rhizobacteria as biological fertilizer is gradually expanding in agriculture as excellent substitutes for chemical fertilizers. Bacillus subtilis SL-44 is a plant growth–promoting rhizobacteria screened from the severely salinized cotton rhizosphere soil in Xinjiang. Study showed that indole-3-acetic acid, organic acid production, nitrogen fixation, and other beneficial secondary metabolite secretion can be synthesized by stain SL-44. At the same time, fencyclin, lipopeptide, chitinase, and other antifungal substances were also detected from the secretion of Bacillus subtilis SL-44, which can effectively control plant diseases. Siderophore separated from SL-44 was verified by HPLC, and results showed it was likely bacillibactin. This study also verified that SL-44 has high antifungal activity against Rhizoctonia solani through in vitro antifungal experiments. The B. subtilis SL-44 whole genome was sequenced and annotated to further explore the biotechnological potential of SL-44. And a large number of genes involved in the synthesis of anti-oxidative stress, antibiotic, and toxins were found. Genome-wide analysis provides clear evidence to support the great potential of B. subtilis SL-44 strain to produce multiple bioantagonistic natural products and growth-promoting metabolites, which may facilitate further research into effective therapies for harmful diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
14. Assessment of the Genetic Diversity and Population Structure of Rhizophora mucronata along Coastal Areas in Thailand.
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Naktang, Chaiwat, Khanbo, Supaporn, Yundaeng, Chutintorn, U-thoomporn, Sonicha, Kongkachana, Wasitthee, Jiumjamrassil, Darunee, Maknual, Chatree, Wanthongchai, Poonsri, Tangphatsornruang, Sithichoke, and Pootakham, Wirulda
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MANGROVE plants , *GENETIC variation , *WHOLE genome sequencing , *SINGLE nucleotide polymorphisms , *RHIZOPHORA , *MANGROVE forests , *MANGROVE ecology , *ECOSYSTEMS - Abstract
Simple Summary: In order to examine the genetic diversity and population structure of the Rhizophora mucronata population in Thailand, we utilized 10× Genomics technology to obtain a comprehensive whole-genome sequence, and restriction site associated DNA sequencing (RAD-seq) to genotype the population. Using SNPs discovered from the R. mucronata genome sequence, we detected moderate levels of genetic diversity and differentiation across 80 R. mucronata accessions collected from the coastal regions of Thailand. Both population structure and principal component analysis (PCA) converged on a clustering of two subpopulations. However, the results of two genetic groups did not correspond to the Gulf of Thailand or the Andaman Sea. Several factors could have influenced the R. mucronata genetic pattern, such as hybridization and anthropogenic factors. Unique and biodiverse, mangrove ecosystems provide humans with benefits and contribute to coastal protection. Rhizophora mucronata, a member of the Rhizophoraceae family, is prevalent in the mangrove forests of Thailand. R. mucronata's population structure and genetic diversity have received scant attention. Here, we sequenced the entire genome of R. mucronata using 10× Genomics technology and obtained an assembly size of 219 Mb with the N50 length of 542,540 bases. Using 2857 single nucleotide polymorphism (SNP) markers, this study investigated the genetic diversity and population structure of 80 R. mucronata accessions obtained from the mangrove forests in Thailand. The genetic diversity of R. mucronata was moderate (I = 0.573, Ho = 0.619, He = 0.391). Two subpopulations were observed and confirmed from both population structure and principal component analysis (PCA). Analysis of molecular variance (AMOVA) showed that there was more variation within populations than between them. Mean pairwise genetic differentiation (FST = 0.09) showed that there was not much genetic difference between populations. Intriguingly, the predominant clustering pattern in the R. mucronata population did not correspond to the Gulf of Thailand and the Andaman Sea, which are separated by the Malay Peninsula. Several factors could have influenced the R. mucronata genetic pattern, such as hybridization and anthropogenic factors. This research will provide important information for the future conservation and management of R. mucronata in Thailand. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
15. The genome of sheep ked (Melophagus ovinus) reveals potential mechanisms underlying reproduction and narrower ecological niches.
- Author
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Zhang, Qingxun, Zhou, Qingsong, Han, Shuyi, Li, Ying, Wang, Ye, and He, Hongxuan
- Subjects
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ECOLOGICAL niche , *OLFACTORY receptors , *MILK proteins , *WHOLE genome sequencing , *SENSORY receptors , *SHEEP - Abstract
Background: Melophagus ovinus is considered to be of great veterinary health significance. However, little is known about the information on genetic mechanisms of the specific biological characteristics and novel methods for controlling M. ovinus. Results: In total, the de novo genome assembly of M. ovinus was 188.421 Mb in size (330 scaffolds, N50 Length: 10.666 Mb), with a mean GC content of 27.74%. A total of 13,372 protein-coding genes were functionally annotated. Phylogenetic analysis indicated that the diversification of M. ovinus and Glossina fuscipes took place 72.76 Mya within the Late Cretaceous. Gene family expansion and contraction analysis revealed that M. ovinus has 65 rapidly-evolving families (26 expansion and 39 contractions) mainly involved DNA metabolic activity, transposases activity, odorant receptor 59a/67d-like, IMD domain-containing protein, and cuticle protein, etc. The universal and tightly conserved list of milk protein orthologues has been assembled from the genome of M. ovinus. Contractions and losses of sensory receptors and vision-associated Rhodopsin genes were significant in M. ovinus, which indicate that the M. ovinus has narrower ecological niches. Conclusions: We sequenced, assembled, and annotated the whole genome sequence of M. ovinus, and launches into the preliminary genetic mechanisms analysis of the adaptive evolution characteristics of M. ovinus. These resources will provide insights to understand the biological underpinnings of this parasite and the disease control strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
16. Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T.
- Author
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Jiang, Lingmin, Seo, Jiyoon, Peng, Yuxin, Jeon, Doeun, Park, Soon Ju, Kim, Cha Young, Kim, Pyoung Il, Kim, Chul Hong, Lee, Ju Huck, and Lee, Jiyoung
- Subjects
- *
PLANT genomes , *PLASMODIOPHORA brassicae , *INDOLEACETIC acid , *BOK choy , *METABOLITES , *IRON clusters , *PEPPERS , *CABBAGE - Abstract
Endophytes can facilitate the improvement of plant growth and health in agriculturally important crops, yet their genomes and secondary metabolites remain largely unexplored. We previously isolated Saccharibacillus brassicae strain ATSA2T from surface-sterilized seeds of kimchi cabbage and represented a novel species of the genus Saccharibacillus. In this study, we evaluated the plant growth-promoting (PGP) effect of strain ATSA2T in kimchi cabbage, bok choy, and pepper plants grown in soils. We found a significant effect on the shoot and root biomass, and chlorophyll contents following strain ATSA2T treatment. Strain ATSA2T displayed PGP traits such as indole acetic acid (IAA, 62.9 μg/mL) and siderophore production, and phosphate solubilization activity. Furthermore, genome analysis of this strain suggested the presence of gene clusters involved in iron acquisition (fhuABD, afuABC, fbpABC, and fepCDG) and phosphate solubilization (pstABCHS, phoABHLU, and phnCDEP) and other phytohormone biosynthesis genes, including indole-3-acetic acid (trpABCDEFG), in the genome. Interestingly, the secondary metabolites cerecidin, carotenoid, siderophore (staphylobactin), and bacillaene underlying plant growth promotion were found in the whole genome via antiSMASH analysis. Overall, physiological testing and genome analysis data provide comprehensive insights into plant growth-promoting mechanisms, suggesting the relevance of strain ATSA2T in agricultural biotechnology. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
17. Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T.
- Author
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Jiang, Lingmin, Seo, Jiyoon, Peng, Yuxin, Jeon, Doeun, Park, Soon Ju, Kim, Cha Young, Kim, Pyoung Il, Kim, Chul Hong, Lee, Ju Huck, and Lee, Jiyoung
- Subjects
PLANT genomes ,PLASMODIOPHORA brassicae ,INDOLEACETIC acid ,BOK choy ,METABOLITES ,IRON clusters ,PEPPERS ,CABBAGE - Abstract
Endophytes can facilitate the improvement of plant growth and health in agriculturally important crops, yet their genomes and secondary metabolites remain largely unexplored. We previously isolated Saccharibacillus brassicae strain ATSA2
T from surface-sterilized seeds of kimchi cabbage and represented a novel species of the genus Saccharibacillus. In this study, we evaluated the plant growth-promoting (PGP) effect of strain ATSA2T in kimchi cabbage, bok choy, and pepper plants grown in soils. We found a significant effect on the shoot and root biomass, and chlorophyll contents following strain ATSA2T treatment. Strain ATSA2T displayed PGP traits such as indole acetic acid (IAA, 62.9 μg/mL) and siderophore production, and phosphate solubilization activity. Furthermore, genome analysis of this strain suggested the presence of gene clusters involved in iron acquisition (fhuABD, afuABC, fbpABC, and fepCDG) and phosphate solubilization (pstABCHS, phoABHLU, and phnCDEP) and other phytohormone biosynthesis genes, including indole-3-acetic acid (trpABCDEFG), in the genome. Interestingly, the secondary metabolites cerecidin, carotenoid, siderophore (staphylobactin), and bacillaene underlying plant growth promotion were found in the whole genome via antiSMASH analysis. Overall, physiological testing and genome analysis data provide comprehensive insights into plant growth-promoting mechanisms, suggesting the relevance of strain ATSA2T in agricultural biotechnology. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
18. Dataset from genome sequencing, assembly and mining of microsatellite markers in barred-button quail (Turnix suscitator)
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Prateek Dey, Swapna Devi Ray, Padmanabhan Pramod, and Ram Pratap Singh
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Whole-genome ,SSR ,Charadriiformes ,Turnix ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Science (General) ,Q1-390 - Abstract
Turnix suscitator (barred-button quail) is a member of the primitive genus Turnix in the highly diverse order of shore birds Charadriiformes. Absence of genome scale data of T. suscitator has limited our understanding about its systematics, taxonomic and evolutionary history as well has hindered the characterization of genome wide microsatellite markers of the same. Hence we generated whole genome short read sequences of T. suscitator, created a high quality assembly and mined genome-wide microsatellite markers from the same. A total of 34142524 reads were sequenced with an estimated genome size of 817 mb. SPAdes assembly consisted of 320761 total contigs and an estimated N50 value of 907 base pairs. Krait identified a total of 77028 microsatellite motifs covering 0.64% of the total sequences in the SPAdes assembly. Further the whole genome sequence and genome wide microsatellites dataset of T. suscitator will facilitate future genomic/evolutionary studies of Turnix species.
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- 2023
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19. Metagenomic sequencing, molecular characterization, and Bayesian phylogenetics of imported type 2 vaccine-derived poliovirus, Spain, 2021
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Maria Dolores Fernandez-Garcia, Martin Faye, Francisco Diez-Fuertes, Antonio Moreno-Docón, Maria Dolores Chirlaque-López, Ousmane Faye, and Maria Cabrerizo
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vaccine-derived polio virus ,whole-genome ,metagenomics ,recombination ,molecular epidemiology ,OPV (oral polio vaccine) ,Microbiology ,QR1-502 - Abstract
IntroductionIn 2021, a type 2 vaccine-derived poliovirus (VDPV2) was isolated from the stool of a patient with acute flaccid paralysis (AFP) admitted to Spain from Senegal. A virological investigation was conducted to characterize and trace the origin of VDPV2.MethodsWe used an unbiased metagenomic approach for the whole-genome sequencing of VDPV2 from the stool (pre-treated with chloroform) and from the poliovirus-positive supernatant. Phylogenetic analyses and molecular epidemiological analyses relying on the Bayesian Markov Chain Monte Carlo methodology were used to determine the geographical origin and estimate the date of the initiating dose of the oral poliovirus vaccine for the imported VDPV2.ResultsWe obtained a high percentage of viral reads per total reads mapped to the poliovirus genome (69.5% for pre-treated stool and 75.8% for isolate) with a great depth of sequencing coverage (5,931 and 11,581, respectively) and complete genome coverage (100%). The two key attenuating mutations in the Sabin 2 strain had reverted (A481G in the 5’UTR and Ile143Thr in VP1). In addition, the genome had a recombinant structure between type-2 poliovirus and an unidentified non-polio enterovirus-C (NPEV-C) strain with a crossover point in the protease-2A genomic region. VP1 phylogenetic analysis revealed that this strain is closely related to VDPV2 strains circulating in Senegal in 2021. According to Bayesian phylogenetics, the most recent common ancestor of the imported VDPV2 could date back 2.6 years (95% HPD: 1.7–3.7) in Senegal. We suggest that all VDPV2s circulating in 2020–21 in Senegal, Guinea, Gambia, and Mauritania have an ancestral origin in Senegal estimated around 2015. All 50 stool samples from healthy case contacts collected in Spain (n = 25) and Senegal (n = 25) and four wastewater samples collected in Spain were poliovirus negative.DiscussionBy using a whole-genome sequencing protocol with unbiased metagenomics from the clinical sample and viral isolate with high sequence coverage, efficiency, and throughput, we confirmed the classification of VDPV as a circulating type. The close genomic linkage with strains from Senegal was consistent with their classification as imported. Given the scarce number of complete genome sequences for NPEV-C in public databases, this protocol could help expand poliovirus and NPEV-C sequencing capacity worldwide.
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- 2023
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20. Whole-Genome Analysis of Starmerella bacillaris CC-PT4 against MRSA, a Non- Saccharomyces Yeast Isolated from Grape.
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Shen, Yong, Bai, Xue, Zhou, Xiran, Wang, Jiaxi, Guo, Na, and Deng, Yanhong
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METHICILLIN-resistant staphylococcus aureus , *WHOLE genome sequencing , *WINE flavor & odor , *GENOME size , *SACCHAROMYCES - Abstract
Starmerella bacillaris is often isolated from environments associated with grape and winemaking. S. bacillaris has many beneficial properties, including the ability to improve the flavor of wine, the production of beneficial metabolites, and the ability to biocontrol. S. bacillaris CC-PT4 (CGMCC No. 23573) was isolated from grape and can inhibit methicillin-resistant Staphylococcus aureus and adaptability to harsh environments. In this paper, the whole genome of S. bacillaris CC-PT4 was sequenced and bioinformatics analyses were performed. The S. bacillaris CC-PT4 genome was finally assembled into five scaffolds with a genome size of 9.45 Mb and a GC content of 39.5%. It was predicted that the strain contained 4150 protein-coding genes, of which two genes encoded killer toxin and one gene encoded lysostaphin. It also contains genes encoding F1F0-ATPases, Na(+)/H(+) antiporter, cation/H(+) antiporter, ATP-dependent bile acid permease, major facilitator superfamily (MFS) antiporters, and stress response protein, which help S. bacillaris CC-PT4 adapt to bile, acid, and other stressful environments. Proteins related to flocculation and adhesion have also been identified in the S. bacillaris CC-PT4 genome. Predicted by antiSMASH, two secondary metabolite biosynthesis gene clusters were found, and the synthesized metabolites may have antimicrobial effects. Furthermore, S. bacillaris CC-PT4 carried genes associated with pathogenicity and drug resistance. Overall, the whole genome sequencing and analysis of S. bacillaris CC-PT4 in this study provide valuable information for understanding the biological characteristics and further development of this strain. [ABSTRACT FROM AUTHOR]
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- 2022
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21. Assessment of the Genetic Diversity and Population Structure of Rhizophora apiculata Blume (Rhizophoraceae) in Thailand.
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Ruang-areerate, Panthita, Naktang, Chaiwat, Kongkachana, Wasitthee, Sangsrakru, Duangjai, Narong, Nattapol, Maknual, Chatree, Pravinvongvuthi, Tamanai, Promchoo, Waratthaya, Yamprasai, Suchart, Tangphatsornruang, Sithichoke, and Pootakham, Wirulda
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MANGROVE plants , *GENETIC variation , *RHIZOPHORA , *MANGROVE forests , *GENOMICS , *GENOMES - Abstract
Simple Summary: We utilized the 10× Genomics technology to obtain a reference whole-genome sequence for assessing the genetic diversity and population structure of Rhizophora apiculata in Thailand. Using SNPs identified from the R. apiculata genome sequence, moderate genetic diversity and high genetic differentiation were observed among 82 R. apiculata accessions collected along the coasts of Thailand. Two subpopulations corresponding to the Gulf of Thailand and the Andaman Sea coasts were clustered and confirmed from three approaches: population structure, PCA, and phylogenetic analyses. The AMOVA result revealed that the percentage of variation within populations (76%) was higher than that among populations (24%). Rhizophora apiculata is one of the most widespread and economically important mangrove trees in the Indo-West Pacific region. Knowledge of the genetic variation of R. apiculata in Thailand is limited. Here, we generated a whole-genome sequence of R. apiculata using the 10× Genomics technology. R. apiculata genome assembly was 230.47 Mb. Based on its genome, 2640 loci of high-quality biallelic SNPs were identified from 82 R. apiculata accessions collected from 17 natural mangrove forests in Thailand to assess the genetic diversity and population structure among them. A moderate level of genetic diversity of R. apiculata was observed. The average observed heterozygosity (Ho = 0.48) was higher than the average expected heterozygosity (He = 0.36). Two subpopulations were observed and confirmed from three approaches: population structure, PCA, and phylogenetic analyses. They corresponded to the Gulf of Thailand and the Andaman Sea separated by the Malay Peninsula. AMOVA analyses indicated that genetic variation was attributable to 76.22% within populations and 23.78% among populations. A high level of genetic differentiation between the two subpopulations (FST = 0.24, p < 0.001) was observed. This study evaluated the genetic diversity and population structure of R. apiculata, providing useful information for sustainable mangrove management in Thailand. [ABSTRACT FROM AUTHOR]
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- 2022
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22. Whole‐genome sequencing and genome‐scale metabolic modeling of Chromohalobacter canadensis 85B to explore its salt tolerance and biotechnological use
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Blaise Manga Enuh, Belma Nural Yaman, Chaimaa Tarzi, Pınar Aytar Çelik, Mehmet Burçin Mutlu, and Claudio Angione
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Chromohalobacter canadensis ,genome‐scale metabolic modeling ,halophiles ,polyhydroxybutyrates ,salt‐tolerant ,whole‐genome ,Microbiology ,QR1-502 - Abstract
Abstract Salt tolerant organisms are increasingly being used for the industrial production of high‐value biomolecules due to their better adaptability compared to mesophiles. Chromohalobacter canadensis is one of the early halophiles to show promising biotechnology potential, which has not been explored to date. Advanced high throughput technologies such as whole‐genome sequencing allow in‐depth insight into the potential of organisms while at the frontiers of systems biology. At the same time, genome‐scale metabolic models (GEMs) enable phenotype predictions through a mechanistic representation of metabolism. Here, we sequence and analyze the genome of C. canadensis 85B, and we use it to reconstruct a GEM. We then analyze the GEM using flux balance analysis and validate it against literature data on C. canadensis. We show that C. canadensis 85B is a metabolically versatile organism with many features for stress and osmotic adaptation. Pathways to produce ectoine and polyhydroxybutyrates were also predicted. The GEM reveals the ability to grow on several carbon sources in a minimal medium and reproduce osmoadaptation phenotypes. Overall, this study reveals insights from the genome of C. canadensis 85B, providing genomic data and a draft GEM that will serve as the first steps towards a better understanding of its metabolism, for novel applications in industrial biotechnology.
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- 2022
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23. Genomic and evolutionary characteristics of G9P[8], the dominant group a rotavirus in China (2016-2018).
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Xiafei Liu, Mengxuan Wang, Shan Li, Jingxin Li, Jinbo Xiao, Huiying Li, Qing Zhang, Xiangyu Kong, Hong Wang, Dandi Li, and Zhaojun Duan
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ROTAVIRUSES ,ROTAVIRUS vaccines ,GENOTYPES ,BAYESIAN analysis - Abstract
G9P[8] became the predominant rotavirus A (RVA) genotype in China in 2012. To evaluate its genetic composition at the whole-genome level, 115 G9P[8] RVA strains isolated from children under 5 years old were sequenced and characterized. All 13 strains in 2016 and 2017 and an additional 54 strains in 2018 were genotyped as G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. The other 48 strains in 2018 were all genotyped as G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1, with the NSP4 gene characterized as a DS-1-like genotype. The time of the most recent common ancestor (tMRCA) and evolution rates of the VP7, VP4, and NSP4 (E1 and E2) genes of these strains were estimated by Bayesian evolutionary dynamics analysis. We estimated the evolution rates (nt substitutions per site per year) as 1.38 × 10
-3 [the 95% highest posterior density (HPD) was 1.09-1.72 × 10-3 ] for VP7, 0.87 × 10-3 (95% HPD: 0.75-1.00 × 10-3 ) for VP4, 0.56 × 10-3 (95% HPD: 0.41-0.73 × 10-3 ) for NSP4-E1, and 1.35 × 10-3 (95% HPD: 0.92-1.86 × 10-3 ) for NSP4-E2. The tMRCA was estimated to be 1935.4 (95% HPD: 1892.4-1961.3) for VP7, 1894.3 (95% HPD: 1850.5-1937.8) for VP4, 1929.4 (95% HPD: 1892.4-1961.3) for NSP4-E1, and 1969.2 (95% HPD: 1942.2-1985.3) for NSP4-E2. The baseline genetic information in this study is expected to improve our understanding of the genomic and evolutionary characteristics of the rotavirus genome. Furthermore, it will provide a basis for the development of next-generation rotavirus vaccines for humans. [ABSTRACT FROM AUTHOR]- Published
- 2022
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24. SARS‐CoV‐2 variants and spike mutations involved in second wave of COVID‐19 pandemic in India.
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Muttineni, Radhakrishna, R.N., Binitha, Putty, Kalyani, Marapakala, Kavitha, K.P., Sandra, Panyam, Jaslin, Vemula, Aravind, Singh, Shashi Mohan, Balachandran, Subin, S.T., Viroji Rao, and Kondapi, Anand Kumar
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SARS-CoV-2 , *COVID-19 pandemic , *SARS-CoV-2 Delta variant , *BREAKTHROUGH infections , *IMMUNOGLOBULINS , *VIRAL mutation - Abstract
Against the backdrop of the second wave of COVID‐19 pandemic in India that started in March 2021, we have monitored the spike (S) protein mutations in all the reported (GISAID portal) whole‐genome sequences of SARS‐CoV‐2 circulating in India from 1 January 2021 to 31 August 2021. In the 43,102 SARS‐CoV‐2 genomic sequences analysed, we have identified 24,260 amino acid mutations in the S protein, based on which 265 Pango lineages could be categorized. The dominant lineage in most of the 28 states of India and its 8 union territories was B.1.617.2 (the delta variant). However, the states Madhya Pradesh, Jammu & Kashmir, and Punjab had B.1.1.7 (alpha variant) as the major lineage, while the Himachal Pradesh state reported B.1.36 as the dominating lineage. A detailed analysis of various domains of S protein was carried out for detecting mutations having a prevalence of >1%; 70, 18, 7, 3, 9, 4, and 1 (N = 112) such mutations were observed in the N‐terminal domain, receptor binding domain, C ‐terminal domain, fusion peptide region, heptapeptide repeat (HR)‐1 domains, signal peptide domain, and transmembrane region, respectively. However, no mutations were recorded in the HR‐2 and cytoplasmic domains of the S protein. Interestingly, 13.39% (N = 15) of these mutations were reported to increase the infectivity and pathogenicity of the virus; 2% (N = 3) were known to be vaccine breakthrough mutations, and 0.89% (N = 1) were known to escape neutralizing antibodies. The biological significance of 82% (N = 92) of the reported mutations is yet unknown. As SARS‐CoV‐2 variants are emerging rapidly, it is critical to continuously monitor local viral mutations to understand national trends of virus circulation. This can tremendously help in designing better preventive regimens in the country, and avoid vaccine breakthrough infections. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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25. Whole-genome sequencing and functional analysis of a novel chitin-degrading strain Rhodococcus sp. 11-3.
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Xiao, Yu, Lu, Haiqiang, Liu, Yang, Sang, Yaxin, and Sun, Jilu
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CHITIN , *NUCLEOTIDE sequencing , *FUNCTIONAL analysis , *RHODOCOCCUS , *SEQUENCE analysis , *WHOLE genome sequencing - Abstract
Chitin is the second most abundant polysaccharide in nature. Therefore, how to utilize the resource is an important issue. Rhodococcus sp. 11-3 is a strain with high chitin deacetylase (CDA) activity. In the present study, we used a combined Illumina and PacBio sequencing strategy to assemble the whole genome sequence of this strain. The genome of Rhodococcus sp. 11-3 was 5,627,661 bp in size and contained 5983 coding genes, of which 5983, 4040, 4648, 4914, 4174, 2350, and 173 genes were annotated in the Non-Redundant Protein Database (NR), Swiss-Prot, Pfam, Clusters of Orthologous Groups of proteins (COG), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Carbohydrate-active enzymes (CAZymes) databases, respectively. The genome was annotated to a chitin deacetylase gene (RhoCDA) and a chitinase gene (RhoChi). They were not very similar to the previously reported chitin deacetylase and chitinase. This made it possible to investigate the genes associated with chitin degradation and would provide an important reference for subsequent gene cloning, functional research, development and application. Therefore, the Rhodococcus sp. 11-3 strain has great potential in the development of chitin resources. [ABSTRACT FROM AUTHOR]
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- 2022
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26. Potential and whole-genome sequence-based mechanism of elongated-prismatic magnetite magnetosome formation in Acidithiobacillus ferrooxidans BYM.
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Zhao, Dan, Yang, Jiani, Zhang, Guojing, Lu, Dong, Zhang, Shuang, Wang, Weidong, and Yan, Lei
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THIOBACILLUS ferrooxidans , *MAGNETITE crystals , *MAGNETITE , *FERROUS sulfate , *GLUCONIC acid - Abstract
A magnetosome-producing bacterium Acidithiobacillus ferrooxidans BYM (At. ferrooxidans BYM) was isolated and magnetically screened. The magnetosome yield from 0.5896 to 13.1291 mg/g was achieved under different aeration rates, ferrous sulfate, ammonium sulfate, and gluconic acid concentrations at 30 ℃. TEM observed 6–9 magnetosomes in size of 20–80 nm irregularly dispersed in a cell. STEM-EDXS and HRTEM-FFT implied that the elongated-prismatic magnetite magnetosomes with {110} crystal faces grown along the [111] direction. Whole-genome sequencing and annotation of BYM showed that 3.2 Mb chromosome and 47.11 kb plasmid coexisted, and 322 genes associated with iron metabolism were discovered. Ten genes shared high similarity with magnetosome genes were predicted, providing sufficient evidence for the magnetosome-producing potential of BYM. Accordingly, we first proposed a hypothetic model of magnetosome formation including vesicle formation, iron uptake and mineralization, and magnetite crystal maturation in At. ferrooxidans. These indicated that At. ferrooxidans BYM would be used as a commercial magnetosome-producing microorganism. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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27. Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T
- Author
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Jiang, Lingmin, Seo, Jiyoon, Peng, Yuxin, Jeon, Doeun, Park, Soon Ju, Kim, Cha Young, Kim, Pyoung Il, Kim, Chul Hong, Lee, Ju Huck, and Lee, Jiyoung
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- 2023
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28. A Review of Pangenome Tools and Recent Studies
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Vernikos, G. S., Tettelin, Hervé, editor, and Medini, Duccio, editor
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- 2020
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29. Molecular Characterization and Phylogenetic Analysis of the 2019 Dengue Outbreak in Wenzhou, China.
- Author
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Han, Axiang, Sun, Baochang, Sun, Zhewei, Xu, Xuelian, Yang, Qiongying, Xie, Danli, Guan, Wanchun, and Lou, Yongliang
- Subjects
COVID-19 ,WHOLE genome sequencing ,DENGUE viruses ,DENGUE ,GENETIC mutation ,AMINO acids ,VIRAL replication - Abstract
In 2019, a dengue outbreak occurred with 290 confirmed cases in Wenzhou, a coastal city in southeast China. To identify the origin of the dengue virus (DENV) from this outbreak, viral RNA was extracted from four serum samples and sequenced for whole genome analysis. Then, phylogenetic analysis, gene mutation, secondary structure prediction, selection pressure analysis, and recombination analysis were performed. DENV strains Cam-03 and Cam-11 were isolated from patients traveling from Cambodia, while ZJWZ-18 and ZJWZ-62 strains were isolated from local patients without a record of traveling abroad. The whole genome sequence of all four strains was 10,735 nucleotides long. Phylogenetic tree analysis showed that the four strains belonged to genotype 1 of DENV-1, but the local Wenzhou strains and imported strains clustered in different branches. ZJWZ-18 and ZJWZ-62 were closely related to strain MF033254-Singapore-2016, and Cam-03 and Cam-11 were closely related to strain AB608788-China : Taiwan-1994. A comparison of the coding regions between the local strains and the DENV-1 standard strain (EU848545-Hawaii-1944) showed 82 amino acid mutations between the two strains. A total of 55 amino acid mutations were found between the coding regions of the local and imported strains. The overall secondary structure of the 3′ UTR of the local strains had changed: apparent changes in the head and tail position were observed when compared to DENV-1 standard strain. Furthermore, selection pressure analysis and recombination detection using the 4 isolates and 41 reference strains showed two credible positive selection sites and eight credible recombination events, which warrant further studies. This study may enhance the understanding of viral replication, infection, evolution, virulence, and pathogenicity of DENV. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
30. Comprehensive Genomic Analysis of Marine Strain Streptomyces sp. 891, an Excellent Producer of Chrysomycin A with Therapeutic Potential.
- Author
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Hu, Xu, Tang, Yuqi, Liu, Yuanyuan, Pei, Xinwei, Huang, Ziwei, Song, Fuhang, and Zhang, Huawei
- Abstract
Chrysomycin A is one of the most promising therapeutic candidates for treating infections caused by multidrug-resistant Gram-positive bacteria. By hybridizing next-step generation (Illumina) and third-generation (PacBio) sequencing technologies, a high-quality chromosome-level genome together with a plasmid was firstly assembled for chrysomycin A-producing marine strain 891. Phylogenetic analysis of the 16S rRNA gene and genome sequences revealed that this strain unambiguously belonged to the genus Streptomyces, and its genomic features and functional genes were comprehensively analyzed and annotated. AntiSMASH analysis of this strain unveiled one key biosynthetic gene cluster, T2PKS, responsible for the biosynthesis of chrysomycin, the biosynthesis pathway of which was putatively proposed. These findings definitely shed light on further investigation for construction of a robust industrial strain with high-yield chrysomycin A production using genetic engineering techniques and combinatorial biology approaches. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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31. Genome-wide analyses of the relict gull (Larus relictus): insights and evolutionary implications
- Author
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Chao Yang, Xuejuan Li, Qingxiong Wang, Hao Yuan, Yuan Huang, and Hong Xiao
- Subjects
Whole-genome ,PacBio sequencing ,Larus relictus ,Habitat loss ,Population fragmentation ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The relict gull (Larus relictus), was classified as vulnerable on the IUCN Red List and is a first-class national protected bird in China. Genomic resources for L. relictus are lacking, which limits the study of its evolution and its conservation. Results In this study, based on the Illumina and PacBio sequencing platforms, we successfully assembled the genome of L. relictus, one of the few known reference genomes in genus Larus. The size of the final assembled genome was 1.21 Gb, with a contig N50 of 8.11 Mb. A total of 18,454 genes were predicted from the assembly results, with 16,967 (91.94%) of these genes annotated. The genome contained 92.52 Mb of repeat sequence, accounting for 7.63% of the assembly. A phylogenetic tree was constructed using 4902 single-copy orthologous genes, which showed L. relictus had closest relative of L. smithsonianus, with divergence time of 14.7 Mya estimated between of them. PSMC analyses indicated that L. relictus had been undergoing a long-term population decline during 0.01-0.1 Mya with a small effective population size fom 8800 to 2200 individuals. Conclusions This genome will be a valuable genomic resource for a range of genomic and conservation studies of L. relictus and will help to establish a foundation for further studies investigating whether the breeding population is a complex population. As the species is threatened by habitat loss and fragmentation, actions to protect L. relictus are suggested to alleviate the fragmentation of breeding populations.
- Published
- 2021
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32. Molecular Characterization and Phylogenetic Analysis of the 2019 Dengue Outbreak in Wenzhou, China
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Axiang Han, Baochang Sun, Zhewei Sun, Xuelian Xu, Qiongying Yang, Danli Xie, Wanchun Guan, and Yongliang Lou
- Subjects
dengue virus ,whole-genome ,phylogenetic analysis ,gene mutation ,molecular characterization ,Microbiology ,QR1-502 - Abstract
In 2019, a dengue outbreak occurred with 290 confirmed cases in Wenzhou, a coastal city in southeast China. To identify the origin of the dengue virus (DENV) from this outbreak, viral RNA was extracted from four serum samples and sequenced for whole genome analysis. Then, phylogenetic analysis, gene mutation, secondary structure prediction, selection pressure analysis, and recombination analysis were performed. DENV strains Cam-03 and Cam-11 were isolated from patients traveling from Cambodia, while ZJWZ-18 and ZJWZ-62 strains were isolated from local patients without a record of traveling abroad. The whole genome sequence of all four strains was 10,735 nucleotides long. Phylogenetic tree analysis showed that the four strains belonged to genotype 1 of DENV-1, but the local Wenzhou strains and imported strains clustered in different branches. ZJWZ-18 and ZJWZ-62 were closely related to strain MF033254-Singapore-2016, and Cam-03 and Cam-11 were closely related to strain AB608788-China : Taiwan-1994. A comparison of the coding regions between the local strains and the DENV-1 standard strain (EU848545-Hawaii-1944) showed 82 amino acid mutations between the two strains. A total of 55 amino acid mutations were found between the coding regions of the local and imported strains. The overall secondary structure of the 3′ UTR of the local strains had changed: apparent changes in the head and tail position were observed when compared to DENV-1 standard strain. Furthermore, selection pressure analysis and recombination detection using the 4 isolates and 41 reference strains showed two credible positive selection sites and eight credible recombination events, which warrant further studies. This study may enhance the understanding of viral replication, infection, evolution, virulence, and pathogenicity of DENV.
- Published
- 2022
- Full Text
- View/download PDF
33. A chromosome-level genome assembly of the red drum, Sciaenops ocellatus
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Tianjun Xu, Ye Li, Qing Chu, and Weiwei Zheng
- Subjects
Red drum ,Whole-genome ,Chromosomal assembly ,Phylogenetic ,Genome evolution ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Sciaenops ocellatus, also known as red drum, is a valuable commercial fish that is widely cultured in China. In recent years, while the genetic research and molecular breeding on S. ocellatus has been widely performed, whole-genome resources are lacking. In our study, we assembled the S. ocellatus genome by high-quality genome sequencing, followed by complete annotation. The size of final genome was 686.62 Mb and N50 length of contig and scaffold is 99.71 Kb and 25.62 Mb, respectively. Using the Hi-C technology, we obtained 24 pseudochromosomes containing 87.82% of the total scaffolds. We annotated 20,053 protein-coding genes in S. ocellatus genome, of which 99.93% were homologous to proteins in different database. Totally, we detected 133.54 Mb repeat sequences, which accounts for 19.45% of the assembly. According to the phylogenetic analysis, S. ocellatus is most closed to L. croea with a divergence time about 30 million years ago. We assembled a high-quality genome that will be a valuable resource to further study the biology of S. ocellatus and will inform how to breed the red drum to enhance its economic traits.
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- 2021
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34. Isolation, identification and characteristics of Bibersteinia trehalosi from goat.
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Guo, Rui, Xu, Mengen, Yang, Keli, Gao, Ting, Zhu, Jiajia, Liu, Wei, Yuan, Fangyan, Liu, Zewen, Li, Chang, Wu, Qiong, Nawaz, Shah, Zhou, Danna, and Tian, Yongxiang
- Subjects
- *
WHOLE genome sequencing , *MICROBIAL sensitivity tests , *GOATS , *MANNHEIMIA haemolytica , *KLEBSIELLA pneumoniae , *GRAM-negative bacteria , *DATABASES - Abstract
A conditionally pathogenic bacterium called Bibersteinia trehalosi inhabits the upper respiratory tract of ruminants and is becoming a significant cause of pneumonia, especially in goats. In this study, we identified a gram-negative bacteria strain isolated from dead goat's lungs, which was named M01. By integrating the outcomes of its morphological and biochemical characterization with the investigation of the 16S rRNA gene sequence analysis, the isolate was identified as B. trehalosi. Based on antibiotic susceptibility tests, the isolate was shown to be resistant to β-lactams, tetracyclines, and amphenicols. Its genome was discovered to comprise 2115 encoded genes and a circular chromosome measuring 2,345,568 bp using whole genome sequencing. Annotation of the VFBD database revealed that isolate M01 had four virulence genes encoding three virulence factors. The CARD database revealed that its genome has two antibiotic-resistance genes. Based on pathogenicity testing, isolate M01 was highly pathogenic to mice, primarily causing pneumonia, with an LD50 of 1.31 × 107 CFU/ml. Moreover, histopathology showed loss of alveolar structure and infiltration of lung inflammatory cells. Hence, the current study could provide sufficient information for prevention and control strategies for future epidemics of B. trehalosi in goat species. • Bibersteinia trehalosi M01 is resistant to β-Lactam, Tetracyclines and Amphenicols. • Bibersteinia trehalosi M01 has four virulence genes, which are cyll, wbaP/rfbP, wbbM, and wbbN. • The LD50 of Bibersteinia trehalosi M01 in mice is 1.31*107 CFU/ml. • This is a rare study of Bibersteinia trehalosi , which is important for the control of Bibersteinia trehalosi in China. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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35. Assessment of the Genetic Diversity and Population Structure of Rhizophora mucronata along Coastal Areas in Thailand
- Author
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Chaiwat Naktang, Supaporn Khanbo, Chutintorn Yundaeng, Sonicha U-thoomporn, Wasitthee Kongkachana, Darunee Jiumjamrassil, Chatree Maknual, Poonsri Wanthongchai, Sithichoke Tangphatsornruang, and Wirulda Pootakham
- Subjects
mangrove ,Rhizophora mucronata ,Rhizophoraceae ,whole-genome ,genetic diversity ,population structure ,Biology (General) ,QH301-705.5 - Abstract
Unique and biodiverse, mangrove ecosystems provide humans with benefits and contribute to coastal protection. Rhizophora mucronata, a member of the Rhizophoraceae family, is prevalent in the mangrove forests of Thailand. R. mucronata’s population structure and genetic diversity have received scant attention. Here, we sequenced the entire genome of R. mucronata using 10× Genomics technology and obtained an assembly size of 219 Mb with the N50 length of 542,540 bases. Using 2857 single nucleotide polymorphism (SNP) markers, this study investigated the genetic diversity and population structure of 80 R. mucronata accessions obtained from the mangrove forests in Thailand. The genetic diversity of R. mucronata was moderate (I = 0.573, Ho = 0.619, He = 0.391). Two subpopulations were observed and confirmed from both population structure and principal component analysis (PCA). Analysis of molecular variance (AMOVA) showed that there was more variation within populations than between them. Mean pairwise genetic differentiation (FST = 0.09) showed that there was not much genetic difference between populations. Intriguingly, the predominant clustering pattern in the R. mucronata population did not correspond to the Gulf of Thailand and the Andaman Sea, which are separated by the Malay Peninsula. Several factors could have influenced the R. mucronata genetic pattern, such as hybridization and anthropogenic factors. This research will provide important information for the future conservation and management of R. mucronata in Thailand.
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- 2023
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36. Paenibacillus arenosi sp. nov., a siderophore-producing bacterium isolated from coastal sediment.
- Author
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Baek, Jihye, Weerawongwiwat, Veeraya, Kim, Jong-Hwa, Yoon, Jung-Hoon, Lee, Jung-Sook, Sukhoom, Ampaitip, and Kim, Wonyong
- Abstract
In this study, strain CAU 1523T, a novel Gram-positive-positive bacterium isolated from marine sediment collected from the coast of Busan, Republic of Korea, was characterized using a polyphasic taxonomic approach. This strain showed growth at a temperature range of 20–37 °C (optimum, 30 °C), a pH range of 6.5–9.5 (optimum, 7.5), and in the presence of 0–3% (w/v) NaCl (optimum, 1%). Phylogenetic analysis based on 16S rRNA gene sequencing and 92 concatenated core genes indicated that CAU 1523T belonged to the genus Paenibacillus, sharing the highest sequence similarity with P. assamensis JCM 13186T (98.0%). CAU 1523T was differentiated from other Paenibacillus species by average nucleotide identity, average amino acid identity, and digital DNA–DNA hybridization values, using cut-off values of 95–96%, 90%, and 70%, respectively, for closely related strains. The genome of CAU 1523T possessed various biosynthetic gene clusters, one of which encoded a putative siderophore-interacting protein. Siderophore production by the isolate was confirmed using the qualitative chrome azurol sulfonate (CAS) agar assay. Based on its phylogenetic and physiological characteristics, strain CAU 1523T represents a novel, siderophore-producing species within the genus Paenibacillus, for which the name Paenibacillus arenosi sp. nov. is proposed, with the type strain CAU 1523T (= KCTC 43108T = MCCC 1K04063T). [ABSTRACT FROM AUTHOR]
- Published
- 2022
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37. Whole-genome analysis of haemophilus influenzae invasive strains isolated from Campinas state University hospital. An epidemiological approach 2012 - 2019 and ancestor strains
- Author
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Rafaella Fabiana Carneiro Pereira, João Paulo de Oliveira Guarnieri, Carlos Fernando Macedo da Silva, Bruno Gaia Bernardes, and Marcelo Lancellotti
- Subjects
Whole-genome ,Haemophilus influenzae ,Sequencing ,Invasive disease ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
Thirteen Haemophylus influenzae invasive strains isolated from patients at Clinical Hospital of State University of Campinas, from May 2013 through August 2019, was submitted to Illumina genome sequencing HiSeq platform. Further in silico analysis of serogroup and Multi Locus Sequence Typing (MLST) from whole DNA sequencing had demonstrated the actual clonal distribution in the Campinas Metropolitan region. Thus, results showed the existence of a new ST Haemophilus influenzae found in the Brazilian territory and an increase of strains belonging to serogroup a (three strains also belonging to ST23). In conclusion, we observed an increase of non-typable H. influenzae (NTHi) and a strain involved in invasive diseases in the Campinas – São Paulo region after frequent detection of those serotypes and genotypes in other Brazilian regions.
- Published
- 2022
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38. Comparative structural analysis of Bru1 region homeologs in Saccharum spontaneum and S. officinarum
- Author
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Ming, Ray [Fujian Agriculture and Forestry Univ., Fuzhou, Fujian Province (China). FAFU and UIUC-SIB Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Inst. of Science and Technology;Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States). Dept. of Plant Biology]
- Published
- 2016
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39. Whole-genome Sequencing of Follicular Thyroid Carcinomas Reveal Recurrent Mutations in MicroRNA Processing Subunit DGCR8.
- Author
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Paulsson, Johan O., Rafati, Nima, DiLorenzo, Sebastian, Yi Chen, Haglund, Felix, Zedenius, Jan, Juhlin, C. Christofer, and Chen, Yi
- Subjects
WHOLE genome sequencing ,MICRORNA ,THYROID cancer ,RNA metabolism ,ADENOCARCINOMA ,PROTEINS ,RESEARCH ,LIVER tumors ,GENETIC mutation ,THYROID gland tumors ,RESEARCH methodology ,PROGNOSIS ,RNA ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,HEPATOCELLULAR carcinoma ,LONGITUDINAL method - Abstract
Background: The genomic and transcriptomic landscape of widely invasive follicular thyroid carcinomas (wiFTCs) and Hürthle cell carcinoma (HCC) are poorly characterized, and subsets of these tumors lack information on genetic driver events.Objective: The aim of this study was to bridge this gap.Methods: We performed whole-genome and RNA sequencing and subsequent bioinformatic analyses of 11 wiFTCs and 2 HCCs with a particularly poor prognosis, and matched normal tissue.Results: All wiFTCs exhibited one or several mutations in established thyroid cancer genes, including TERT (n = 4), NRAS (n = 3), HRAS, KRAS, AKT, PTEN, PIK3CA, MUTYH, TSHR, and MEN1 (n = 1 each). MutSig2CV analysis revealed recurrent somatic mutations in FAM72D (n = 3, in 2 wiFTCs and in a single HCC), TP53 (n = 3, in 2 wiFTCs and a single HCC), and EIF1AX (n = 3), with DGCR8 (n = 2) as borderline significant. The DGCR8 mutations were recurrent p.E518K missense alterations, known to cause familial multinodular goiter via disruption of microRNA (miRNA) processing. Expression analyses showed reduced DGCR8 messenger RNA expression in FTCs in general, and the 2 DGCR8 mutants displayed a distinct miRNA profile compared to DGCR8 wild-types. Copy number analyses revealed recurrent gains on chromosomes 4, 6, and 10, and fusion gene analyses revealed 27 high-quality events. Both HCCs displayed hyperploidy, which was fairly unusual in the FTC cohort. Based on the transcriptome data, tumors amassed in 2 principal clusters.Conclusion: We describe the genomic and transcriptomic landscape in wiFTCs and HCCs and identify novel recurrent mutations and copy number alterations with possible driver properties and lay the foundation for future studies. [ABSTRACT FROM AUTHOR]- Published
- 2021
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40. Assessing biological factors affecting postspeciation introgression
- Author
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Jennafer A. P. Hamlin, Mark S. Hibbins, and Leonie C. Moyle
- Subjects
Geographic proximity ,hybridization ,mating system variation ,Solanum ,tomato ,whole‐genome ,Evolution ,QH359-425 - Abstract
Abstract An increasing number of phylogenomic studies have documented a clear “footprint” of postspeciation introgression among closely related species. Nonetheless, systematic genome‐wide studies of factors that determine the likelihood of introgression remain rare. Here, we propose an a priori hypothesis‐testing framework that uses introgression statistics—including a new metric of estimated introgression, Dp—to evaluate general patterns of introgression prevalence and direction across multiple closely related species. We demonstrate this approach using whole genome sequences from 32 lineages in 11 wild tomato species to assess the effect of three factors on introgression—genetic relatedness, geographical proximity, and mating system differences—based on multiple trios within the “ABBA–BABA” test. Our analyses suggest each factor affects the prevalence of introgression, although our power to detect these is limited by the number of comparisons currently available. We find that of 14 species pairs with geographically “proximate” versus “distant” population comparisons, 13 showed evidence of introgression; in 10 of these cases, this was more prevalent between geographically closer populations. We also find modest evidence that introgression declines with increasing genetic divergence between lineages, is more prevalent between lineages that share the same mating system, and—when it does occur between mating systems—tends to involve gene flow from more inbreeding to more outbreeding lineages. Although our analysis indicates that recent postspeciation introgression is frequent in this group—detected in 15 of 17 tested trios—estimated levels of genetic exchange are modest (0.2–2.5% of the genome), so the relative importance of hybridization in shaping the evolutionary trajectories of these species could be limited. Regardless, similar clade‐wide analyses of genomic introgression would be valuable for disentangling the major ecological, reproductive, and historical determinants of postspeciation gene flow, and for assessing the relative contribution of introgression as a source of genetic variation.
- Published
- 2020
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41. Replicate whole-genome next-generation sequencing data derived from Caucasian donor saliva samples
- Author
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Marcus Høy Hansen and Charlotte Guldborg Nyvold
- Subjects
Whole-genome ,Homo Sapiens genome ,Next-generation sequencing (NGS) ,DNA sequencing ,Raw data replicate ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Science (General) ,Q1-390 - Abstract
Next-generation sequencing (NGS) of whole genomes has become more accessible to biomedical researchers as the sequencing price continues to drop, and more laboratories have NGS facilities or have access to a core facility. However, the rapid and robust development of practical bioinformatics pipelines partly depends on convenient access to data for the testing of algorithms. Publicly available data sets constitute a part of this strategy.Here, we provide a triplicate whole-genome paired-end sequencing data set, consisting of 1.38 billion raw sequencing reads derived from saliva DNA from a single anonymous male Caucasian donor, with the average sequencing depths aimed at 30x for two of the samples and 4x for a low-coverage sample. The raw number of single nucleotide variants were 3.3–4 million and the median variant read depth of GATK4-passed variants in three samples was 22, 18, and 10. 81% of all variants were found in two or three of the samples, whereas 19% were singletons. The karyotype was evaluated as 46,XY with no apparent copy-number variation.The data set is provided without restrictions for research, educational or commercial purposes.
- Published
- 2021
- Full Text
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42. Subtype-WGME enables whole-genome-wide multi-omics cancer subtyping.
- Author
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Yang H, Zhao L, Li D, An C, Fang X, Chen Y, Liu J, Xiao T, and Wang Z
- Subjects
- Humans, Algorithms, Prognosis, Genome-Wide Association Study methods, Computational Biology methods, Genome, Human genetics, Multiomics, Neoplasms genetics, Neoplasms classification, Genomics methods, Biomarkers, Tumor genetics
- Abstract
We present an innovative strategy for integrating whole-genome-wide multi-omics data, which facilitates adaptive amalgamation by leveraging hidden layer features derived from high-dimensional omics data through a multi-task encoder. Empirical evaluations on eight benchmark cancer datasets substantiated that our proposed framework outstripped the comparative algorithms in cancer subtyping, delivering superior subtyping outcomes. Building upon these subtyping results, we establish a robust pipeline for identifying whole-genome-wide biomarkers, unearthing 195 significant biomarkers. Furthermore, we conduct an exhaustive analysis to assess the importance of each omic and non-coding region features at the whole-genome-wide level during cancer subtyping. Our investigation shows that both omics and non-coding region features substantially impact cancer development and survival prognosis. This study emphasizes the potential and practical implications of integrating genome-wide data in cancer research, demonstrating the potency of comprehensive genomic characterization. Additionally, our findings offer insightful perspectives for multi-omics analysis employing deep learning methodologies., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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43. Whole-Genome Analysis of Starmerella bacillaris CC-PT4 against MRSA, a Non-Saccharomyces Yeast Isolated from Grape
- Author
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Yong Shen, Xue Bai, Xiran Zhou, Jiaxi Wang, Na Guo, and Yanhong Deng
- Subjects
Starmerella bacillaris ,whole-genome ,secondary metabolite ,lysostaphin ,Biology (General) ,QH301-705.5 - Abstract
Starmerella bacillaris is often isolated from environments associated with grape and winemaking. S. bacillaris has many beneficial properties, including the ability to improve the flavor of wine, the production of beneficial metabolites, and the ability to biocontrol. S. bacillaris CC-PT4 (CGMCC No. 23573) was isolated from grape and can inhibit methicillin-resistant Staphylococcus aureus and adaptability to harsh environments. In this paper, the whole genome of S. bacillaris CC-PT4 was sequenced and bioinformatics analyses were performed. The S. bacillaris CC-PT4 genome was finally assembled into five scaffolds with a genome size of 9.45 Mb and a GC content of 39.5%. It was predicted that the strain contained 4150 protein-coding genes, of which two genes encoded killer toxin and one gene encoded lysostaphin. It also contains genes encoding F1F0-ATPases, Na(+)/H(+) antiporter, cation/H(+) antiporter, ATP-dependent bile acid permease, major facilitator superfamily (MFS) antiporters, and stress response protein, which help S. bacillaris CC-PT4 adapt to bile, acid, and other stressful environments. Proteins related to flocculation and adhesion have also been identified in the S. bacillaris CC-PT4 genome. Predicted by antiSMASH, two secondary metabolite biosynthesis gene clusters were found, and the synthesized metabolites may have antimicrobial effects. Furthermore, S. bacillaris CC-PT4 carried genes associated with pathogenicity and drug resistance. Overall, the whole genome sequencing and analysis of S. bacillaris CC-PT4 in this study provide valuable information for understanding the biological characteristics and further development of this strain.
- Published
- 2022
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- View/download PDF
44. Complete genome sequence of Chlamydia psittaci АМК-16, isolated from a small ruminant in the Middle Volga Region, Russia.
- Author
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Zaitsev S, Khizhnyakova M, Saltykov Y, Evstifeev V, Khusainov F, Ivanova S, Morozova D, Yakovlev S, Larionova O, and Feodorova V
- Abstract
We report the complete genome sequence of the Chlamydia psittaci АМК-16, recovered from the aborted caprine fetus during a case of chlamydia infection. This 1,152,497-bp genome with 7,552-bp cryptic plasmid provides novel insights into the genetic diversity of chlamydia agent strains particularly those causing the infection in small ruminants., Competing Interests: The authors declare no conflict of interest.
- Published
- 2024
- Full Text
- View/download PDF
45. Assessment of the Genetic Diversity and Population Structure of Rhizophora apiculata Blume (Rhizophoraceae) in Thailand
- Author
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Panthita Ruang-areerate, Chaiwat Naktang, Wasitthee Kongkachana, Duangjai Sangsrakru, Nattapol Narong, Chatree Maknual, Tamanai Pravinvongvuthi, Waratthaya Promchoo, Suchart Yamprasai, Sithichoke Tangphatsornruang, and Wirulda Pootakham
- Subjects
mangrove ,Rhizophora apiculata ,Rhizophoraceae ,whole-genome ,genetic diversity ,population structure ,Biology (General) ,QH301-705.5 - Abstract
Rhizophora apiculata is one of the most widespread and economically important mangrove trees in the Indo-West Pacific region. Knowledge of the genetic variation of R. apiculata in Thailand is limited. Here, we generated a whole-genome sequence of R. apiculata using the 10× Genomics technology. R. apiculata genome assembly was 230.47 Mb. Based on its genome, 2640 loci of high-quality biallelic SNPs were identified from 82 R. apiculata accessions collected from 17 natural mangrove forests in Thailand to assess the genetic diversity and population structure among them. A moderate level of genetic diversity of R. apiculata was observed. The average observed heterozygosity (Ho = 0.48) was higher than the average expected heterozygosity (He = 0.36). Two subpopulations were observed and confirmed from three approaches: population structure, PCA, and phylogenetic analyses. They corresponded to the Gulf of Thailand and the Andaman Sea separated by the Malay Peninsula. AMOVA analyses indicated that genetic variation was attributable to 76.22% within populations and 23.78% among populations. A high level of genetic differentiation between the two subpopulations (FST = 0.24, p < 0.001) was observed. This study evaluated the genetic diversity and population structure of R. apiculata, providing useful information for sustainable mangrove management in Thailand.
- Published
- 2022
- Full Text
- View/download PDF
46. MinION Whole-Genome Sequencing in Resource-Limited Settings: Challenges and Opportunities
- Author
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Wasswa, Fredrickson B., Kassaza, Kennedy, Nielsen, Kirsten, and Bazira, Joel
- Published
- 2022
- Full Text
- View/download PDF
47. BitmapAligner: Bit-Parallelism String Matching with MapReduce and Hadoop.
- Author
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Aksa, Mary, Rashid, Junaid, Nisar, Muhammad Wasif, Mahmood, Toqeer, Hyuk-Yoon Kwon, and Hussain, Amir
- Subjects
SEQUENCE alignment ,SPACE (Architecture) ,DATA warehousing ,PATTERN matching ,INDUSTRIAL costs ,DATA structures - Abstract
Advancements in next-generation sequencer (NGS) platforms have improved NGS sequence data production and reduced the cost involved, which has resulted in the production of a large amount of genome data. The downstream analysis of multiple associated sequences has become a bottleneck for the growing genomic data due to storage and space utilization issues in the domain of bioinformatics. The traditional string-matching algorithms are efficient for small sized data sequences and cannot process large amounts of data for downstream analysis. This study proposes a novel bit-parallelism algorithm called BitmapAligner to overcome the issues faced due to a large number of sequences and to improve the speed and quality of multiple sequence alignment (MSA). The input files (sequences) tested over BitmapAligner can be easily managed and organized using the Hadoop distributed file system. The proposed aligner converts the test file (the whole genome sequence) into binaries of an equal length of the sequence, line by line, before the sequence alignment processing. The Hadoop distributed file system splits the larger files into blocks, based on a defined block size, which is 128MB by default. BitmapAligner can accurately process the sequence alignment using the bitmask approach on large-scale sequences after sorting the data. The experimental results indicate that BitmapAligner operates in real time, with a large number of sequences. Moreover, BitmapAligner achieves the exact start and end positions of the pattern sequence to test the MSA application in the whole genome query sequence. The MSA's accuracy is verified by the bitmask indexing property of the bit-parallelism extended shifts (BXS) algorithm. The dynamic and exact approach of the BXS algorithm is implemented through the MapReduce function of Apache Hadoop. Conversely, the traditional seeds-and-extend approach faces the risk of errors while identifying the pattern sequences' positions. Moreover, the proposed model resolves the largescale data challenges that are covered through MapReduce in the Hadoop framework. Hive, Yarn, HBase, Cassandra, and many other pertinent flavors are to be used in the future for data structuring and annotations on the top layer of Hadoop since Hadoop is primarily used for data organization and handles text documents. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
48. Whole‐genome analysis to describe a human adenovirus D8 conjunctivitis outbreak in a tertiary hospital.
- Author
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Miro, Elisenda, Del Cuerpo, Margarita, Rubio, Marc, Berengua, Carla, Español, Montserrat, Marin, Pilar, Vela, Jose I., Pomar, Virginia, Gutierrez, Cristina, Navarro, Ferran, and Rabella, Núria
- Subjects
CONJUNCTIVITIS ,KERATOCONJUNCTIVITIS ,ADENOVIRUSES ,EYE diseases ,HUMAN adenoviruses ,POLYMERASE chain reaction - Abstract
Conjunctivitis is a frequent ocular disorder caused by human adenoviruses (HAdVs). Only a few of the 45 HAdV‐D species are associated with epidemic keratoconjunctivitis, including HAdV‐D8. Nosocomial outbreaks due to HAdV‐D8 have been rarely described, because keratoconjunctivitis cases are clinically diagnosed and treated without having to characterize the causative agent. Moreover, molecular typing is tedious when using classical techniques. In this study, a hospital outbreak of conjunctivitis caused by HAdV‐D8 was characterized using the recently developed whole‐genome sequencing (WGS) method. Of the 363 patients attending the Ophthalmology Department between July 13 and August 13, 2018, 36 may have acquired intrahospital conjunctivitis. Also, 11 of 22 samples sent to the Virology section were selected for WGS analysis. The WGS results revealed that 10 out of 11 HAdV‐D8 strains were closely related. The remaining strain (Case 28) was more similar to a strain from an outbreak in Germany obtained from a public sequence database. WGS results showed that outbreak HAdV‐D8 strains had a minimum percentage of identity of 94.3%. WGS is useful in a clinical setting, because it avoids carrying out viral culture or specific polymerase chain reaction sequencing. The public availability of sequence reads makes it easier to compare clusters in circulation. In conclusion, WGS can play an important role in standard routines to describe viral outbreaks. Highlights: ‐Keratoconjunctivitis is a severe infectious eye disease associated with HAdV‐D8. ‐The HAdV‐D8 genome is highly conserved and WGS analysis is more exhaustive than other conventional methods. ‐WGS and phylogenetic analysis revealed a monophyletic cluster of patients infected with HAdV‐D8. ‐The whole‐genome approach has demonstrated the utility of adenovirus sequencing in a clinical setting. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
49. Insights into salvianolic acid B biosynthesis from chromosome‐scale assembly of the Salvia bowleyana genome.
- Author
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Zheng, Xuehai, Chen, Duo, Chen, Binghua, Liang, Limin, Huang, Zhen, Fan, Wenfang, Chen, Jiannan, He, Wenjin, Chen, Huibin, Huang, Luqiang, Chen, Youqiang, Zhu, Jinmao, and Xue, Ting
- Subjects
- *
SALVIA miltiorrhiza , *SALVIA , *BIOSYNTHESIS , *GENOMES , *DIETARY supplements , *GENE families - Abstract
Salvia bowleyana is a traditional Chinese medicinal plant that is a source of nutritional supplements rich in salvianolic acid B and a potential experimental system for the exploration of salvianolic acid B biosynthesis in the Labiatae. Here, we report a high‐quality chromosome‐scale genome assembly of S. bowleyana covering 462.44 Mb, with a scaffold N50 value of 57.96 Mb and 44,044 annotated protein‐coding genes. Evolutionary analysis revealed an estimated divergence time between S. bowleyana and its close relative S. miltiorrhiza of ~3.94 million years. We also observed evidence of a whole‐genome duplication in the S. bowleyana genome. Transcriptome analysis showed that SbPAL1 (PHENYLALANINE AMMONIA‐LYASE1) is highly expressed in roots relative to stem and leaves, paralleling the location of salvianolic acid B accumulation. The laccase gene family in S. bowleyana outnumbered their counterparts in both S. miltiorrhiza and Arabidopsis thaliana, suggesting that the gene family has undergone expansion in S. bowleyana. Several laccase genes were also highly expressed in roots, where their encoded proteins may catalyze the oxidative reaction from rosmarinic acid to salvianolic acid B. These findings provide an invaluable genomic resource for understanding salvianolic acid B biosynthesis and its regulation, and will be useful for exploring the evolution of the Labiatae. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
50. Polymorphisms in canine immunoglobulin heavy chain gene cluster: a double‐edged sword for diabetes mellitus in the dog.
- Author
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Sanz, C. R., Sevane, N., Pérez‐Alenza, M. D., Valero‐Lorenzo, M., and Dunner, S.
- Subjects
- *
IMMUNOGLOBULIN heavy chains , *GENE clusters , *DIABETES , *TYPE 1 diabetes , *ENDOCRINE diseases - Abstract
Summary: Insulin deficiency diabetes (IDD) in dogs is an endocrine disease similar to human type 1 diabetes. There are breeds more commonly affected, such as Yorkshire Terrier and Samoyed, suggesting an underlying genetic component. However, the genetic basis for canine diabetes mellitus (DM) is not fully established. We conducted both whole‐genome scans for selection signatures and GWASs to compare the genomes of 136 dogs belonging to 29 breeds previously described at low or high risk for developing DM. Candidate variants were tested in dogs with a diagnosis of IDD and controls attending the Complutense Veterinary Teaching Hospital. The only genomic region under selection (CFA8:72 700 000–74 600 000; CanFam3.1) retrieved by our analyses is included in the immunoglobulin heavy chain gene cluster, which has already been related to human human type 1 diabetes susceptibility. This region contains two non‐synonymous variants, rs852072969 and rs851728071, showing significant associations with high or low risk for IDD, respectively. The first variant, rs852072969, alters a protein poorly characterised in the dog. In contrast, rs851728071 was predicted to block the synthesis of an immunoglobulin variable (V) domain in breeds at low risk for DM. Although a large and diverse V gene repertoire is thought to offer a fitness advantage, we suggest that rs851728071 prevents the formation of an auto‐reactive immunoglobulin V domain probably involved in the pathophysiology of IDD and, thus, decreases the risk for the disease. These results should be interpreted with caution until the functional roles of the proposed variants have been proved in larger studies. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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