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Curcumin and Zinc oxide nanoparticle (ZnO-NP) are known as organic and mineral wound healing agents, respectively. In this study, we aimed to green-synthesize the curcumin-mediated ZnO-NP (CmZnO-NP) and evaluate biological properties. In this study, CmZnO-NP were synthesized and after characterizing (DLS, XRD, FT IR, FESEM) its antioxidant effects (ABTS, DPP H), toxicity (MTT) and wound healing (H&E staining and gene expression) were investigated. The 30.77-nm CmZnO-NP significantly scavenged both free radicals. Also, its wound healing activity was observed by detecting the meaningful reduction in the length and depth of the rat's skin wound. The collagen and VEGF up-regulation of the rat skin tissue following the increasing CmZnO-NP treatment doses confirmed its wound healing potential. Due to antioxidant and improved wound healing activities of the CmZnO-NP, it has the potential to be studied as an efficient wound healing compound. [ABSTRACT FROM AUTHOR]

Chromolaena odorata (L.), locally named Siam weed, is a rich source of phyto-bioactives and has been traditionally used for wound healing. This study deals with the phytochemical investigation of the leaves of C. odorata (L.) for their antioxidant, anti-inflammatory, and antibacterial properties that contribute to the observed wound healing properties. The phytoconstituents were sequentially extracted from the powdered leaves of C. odorata (L.) and chemoprofiled using the LC-MS technique. Furthermore, copper oxide/copper nanoparticles (CuNPs) were synthesized by the use of water extracts from the leaves of C. odorata (L.), characterized and evaluated for their potential biological and wound healing impacts. In this relation, scutellarein, isosakuranetin, and rutin, along with 15 other phytoconstituents were identified. Higher antioxidant (79.2% at 200 µg/mL) and anti-inflammatory potentials (66.8%) were obtained for CuNPs (120 µg/mL), in the size range of 66.8 ± 24.8 nm. Antibacterial activity against methicillin-resistant Staphylococcus aureus and Pseudomonas sp. demonstrated mean zones of inhibition of 16.7 ± 4 mm and 17.73 ± 2 mm, respectively. Hence, the synthesized CuNPs would be desirable for inclusion in nanofibrous medical dressings. [ABSTRACT FROM AUTHOR]

This study aimed at the relationship between antioxidant capacity, antimicrobial activity, and in vitro evaluation of the wound healing effect of the extract obtained from Echinaceae purpureae folium (EPF). This study's objective was to assess the bioactive components (total phenol and flavonoid content) and antioxidant activity of EPF extracts using the DPPH test method. The antioxidant capacity and the quantities of the compounds with antioxidant capacity were evaluated by spectrophotometric methods. Antimicrobial activity has been investigated against various pathogenic microorganisms. The minimum inhibitory concentration was determined by the microdilution method. Additionally, our work used a scratch test to examine the in vitro wound healing effects of EPF extract on NHDF cells. Statistical analysis was used to quantify the rate of migration and proliferation of fibroblast cells within the wound. Microscope pictures of fibroblast cells exposed to various EPF extract dosages were processed to estimate the width of the wound, area of the wound, and cell density inside the wound. The study proved that there was a relationship between the antioxidant, antimicrobial, and wound healing ability of EPF extracts. [ABSTRACT FROM AUTHOR]

This study aimed at the relationship between antioxidant capacity, antimicrobial activity, and in vitro evaluation of the wound healing effect of the extract obtained from Echinaceae purpureae folium (EPF). This study’s objective was to assess the bioactive components (total phenol and flavonoid content) and antioxidant activity of EPF extracts using the DPPH test method. The antioxidant capacity and the quantities of the compounds with antioxidant capacity were evaluated by spectrophotometric methods. Antimicrobial activity has been investigated against various pathogenic microorganisms. The minimum inhibitory concentration was determined by the microdilution method. Additionally, our work used a scratch test to examine the in vitro wound healing effects of EPF extract on NHDF cells. Statistical analysis was used to quantify the rate of migration and proliferation of fibroblast cells within the wound. Microscope pictures of fibroblast cells exposed to various EPF extract dosages were processed to estimate the width of the wound, area of the wound, and cell density inside the wound. The study proved that there was a relationship between the antioxidant, antimicrobial, and wound healing ability of EPF extracts.

Snail mucus is rich in proteins and polysaccharides, which has been proved to promote wound healing in mice in our previous research. The aim of this study was to investigate the effective component in snail mucus that can exert the wound healing potential and its structural characterization. Here, the glycoprotein from the snail mucus (SM1S) was obtained by DEAE-Sepharose Fast Flow and Sephacryl S-300 columns. The structural characteristics of SM1S were investigated via chromatographic techniques, periodic acid oxidation, FT-IR spectroscopy and NMR spectroscopy. Results showed that SM1S was a glycoprotein with a molecular weight of 3.8 kDa (83.23 %), consists of mannose, glucuronic acid, glucose, galactose, xylose, arabinose, fucose at a ratio of 13.180:4.875:1043.173:7.552:1:3.501:2.058. In addition, the periodic acid oxidation and NMR analysis showed that SM1S contained 1,6-glycosidic bonds, and might also contain 1 → 4 and 1 → 2 glycosidic or 1 → 3 glycosidic bonds. Furthermore, the migration experiment of human skin fibroblasts in vitro suggested that SM1S had a good effect to accelerate the scratch healing of cells. This study suggested that SM1S may be a prospective candidate as a natural wound dressing for the development of snail mucus products. [Display omitted] [ABSTRACT FROM AUTHOR]

Treatment of wounds by ethnomedicinal plants which have fewer side effects than chemical drugs has been on the rise. In this experiment, we evaluated cutaneous wound healing potential of aqueous extract of Falcaria vulgaris in Wistar male rats. DPPH free radical scavenging test was used to examine the antioxidant effect of F. vulgaris aqueous extract, which indicated high antioxidant activity compared to butylated hydroxy toluene (BHT) as the positive control. In our study, after creating the cutaneous wound on the back of the rats, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, treatment with 3% F. vulgaris aqueous extract ointment. The groups were treated for 30 days. For histopathological and biochemical analysis of the cutaneous wound healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at days 10, 20, and 30. The use of F. vulgaris aqueous extract ointment in the treatment groups led to significant decrease (p < 0.05) in the levels of wound area, total cells, lymphocyte, neutrophil, macrophage, and significant enhance (p < 0.05) in the levels of wound contracture, hydroxyproline, hexosamine, fibrocyte, fibroblast, and the rate of fibrocyte to fibroblast as compared to the control and basal ointment groups. According to the results, F. vulgaris aqueous extract ointment can treat the cutaneous wound. [ABSTRACT FROM AUTHOR]

Background: Emerging evidence indicates that mesenchymal stromal cells (MSCs) isolated from different tissue sources may be used in vivo as tissue restorative agents. To date, there is no evidence, however, on migration and proliferation ("wound healing") potential of different subsets of MSCs. The main goal of this study was therefore to compare the in vitro "wound healing" capacity of MSCs generated from positively selected CD271(+) bone marrow mononuclear cells (CD271-MSCs) and MSCs generated by plastic adherence (PA-MSCs).Methods: The in vitro model of wound healing (CytoSelect™ 24-Well Wound Healing Assay) was used in order to compare the migration and proliferation potential of CD271-MSCs and PA-MSCs of passage 2 and 4 cultured in presence or absence of growth factors or cytokines.Results: CD271-MSCs of both passages when compared to PA-MSCs demonstrated a significantly higher potential to close the wound 12 and 24 h after initiation of the wound healing assay (P < 0.003 and P < 0.002, respectively). Noteworthy, the migration capacity of PA-MSCs of second passage was significantly improved after stimulation with FGF-2 (P < 0.02), PDGF-BB (P < 0.006), MCP-1 (P < 0.002) and IL-6 (P < 0.03), whereas only TGF-β enhanced significantly migration process of PA-MSCs of P4 12 h after the treatment (P < 0.02). Interestingly, treatment of CD271-MSCs of both passages with growth factors or cytokines did not affect their migratory potential.Conclusions: Our in vitro data provide the first evidence that CD271-MSCs are significantly more potent in "wound healing" than their counterparts PA-MSCs. [ABSTRACT FROM AUTHOR]

Members of the Prosopis genus are native to America, Africa and Asia, and have long been used in traditional medicine. The Prosopis species most commonly used for medicinal purposes are P. africana , P. alba , P. cineraria , P. farcta , P. glandulosa , P. juliflora , P. nigra , P. ruscifolia and P. spicigera , which are highly effective in asthma, birth/postpartum pains, callouses, conjunctivitis, diabetes, diarrhea, expectorant, fever, flu, lactation, liver infection, malaria, otitis, pains, pediculosis, rheumatism, scabies, skin inflammations, spasm, stomach ache, bladder and pancreas stone removal. Flour, syrup, and beverages from Prosopis pods have also been potentially used for foods and food supplement formulation in many regions of the world. In addition, various in vitro and in vivo studies have revealed interesting antiplasmodial, antipyretic, anti-inflammatory, antimicrobial, anticancer, antidiabetic and wound healing effects. The phytochemical composition of Prosopis plants, namely their content of C-glycosyl flavones (such as schaftoside, isoschaftoside, vicenin II, vitexin and isovitexin) has been increasingly correlated with the observed biological effects. Thus, given the literature reports, Prosopis plants have positive impact on the human diet and general health. In this sense, the present review provides an in-depth overview of the literature data regarding Prosopis plants' chemical composition, pharmacological and food applications, covering from pre-clinical data to upcoming clinical studies., Competing Interests: The authors declare no conflict of interest.