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The elevated expression of Th17-related cytokines and receptors is associated with skin lesion severity in early systemic sclerosis.
- Source :
-
Human Immunology . Jan2015, Vol. 76 Issue 1, p22-29. 8p. - Publication Year :
- 2015
-
Abstract
- Objective The objective was to survey the expression and localization of Th17-related cytokines and their correlation with skin lesion severity in early systemic sclerosis (SSc). Methods The mRNA expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR) from 21 SSc patients and 12 healthy controls (HC). The protein expression was examined by immunohistochemistry (IHC) and Western blotting. Results The RT-qPCR analysis showed a significantly higher expression of IL-17A, IL-21, IL-22, IL-26, IL-17RA, IL-21R, and IL-22R1 mRNA; consistently, the IHC analysis showed an over-expression of IL-17RA, IL-21R and IL-22R1 and the Western blotting analysis showed an over-expression of IL-17A, IL-21, IL-21R and IL-22R1 in early SSc skin lesions. The mRNA levels of IL-21 were higher in diffuse cutaneous than limited cutaneous SSc lesions. The mRNA expression of IL-26, IL-22, IL-22R1, mRNA and protein expression of IL-17A, IL-21, IL-21R were positively correlated with the modified Rodnan skin score of SSc. In addition, the mRNA levels of ICAM-1 were positively correlated with IL-17A/IL-17RA, and VEGFA and IL-4 were both positively correlated with IL-21/IL-21R, while TGF-β were moderately negatively correlated with IL-22/IL-22R1. Conclusions Th17 cytokines contribute to progression in early SSc skin lesions. IL-21/IL-21R could act as potential biomarkers presenting early SSc skin lesions severity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01988859
- Volume :
- 76
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Human Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 100236292
- Full Text :
- https://doi.org/10.1016/j.humimm.2014.12.008