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microRNA133a Targets Foxl2 and Promotes Differentiation of C2C12 into Myogenic Progenitor Cells.
- Source :
-
DNA & Cell Biology . Jan2015, Vol. 34 Issue 1, p29-36. 8p. 1 Color Photograph, 1 Chart, 4 Graphs. - Publication Year :
- 2015
-
Abstract
- microRNAs are endogenous noncoding RNA molecules of ∼22 nucleotides that regulate gene function by modification of target mRNAs. Due to tissue specific of miR-133a and miR-1/206 for skeletal muscles, we investigated the role of miR-133a and miR-1/206 in promoting the differentiation of the C2C12 cells. The results show that directly transfecting mature miR-133a, miR-1/206, or combinations (miR-1 and miR-206, miR-1 and miR-133a, and miR-133a and miR-206) into C2C12 cells, respectively, for 5 days induces formation of myogenic progenitor cells. Overexpression of miR-133a and miR-206 in C2C12 cells greatly improved multinucleated myotube formation. microRNA-133a (miR-133a) is highly expressed during human muscle development. Using bioinformatics, we identified one putative miR-133a binding site within the 3′-untranslated region of the mouse Foxl2 mRNA. The expression of Foxl2 was shown to be downregulated by subsequent western blot analysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10445498
- Volume :
- 34
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- DNA & Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 100237448
- Full Text :
- https://doi.org/10.1089/dna.2014.2522