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Finding the Switch: Turning a Baeyer-Villiger Monooxygenase into a NADPH Oxidase.
- Source :
-
Journal of the American Chemical Society . 12/10/2014, Vol. 136 Issue 49, p16966-16969. 4p. - Publication Year :
- 2014
-
Abstract
- By a targeted enzyme engineering approach, we were able to create an efficient NADPH oxidase from a monooxygenase. Intriguingly, replacement of only one specific single amino acid was sufficient for such a monooxygenase-to-oxidase switch--a complete transition in enzyme activity. Pre-steady-state kinetic analysis and elucidation of the crystal structure of the C65D PAMO mutant revealed that the mutation introduces small changes near the flavin cofactor, resulting in a rapid decay of the peroxyflavin intermediate. The engineered biocatalyst was shown to be a thermostable, solvent tolerant, and effective cofactor-regenerating biocatalyst. Therefore, it represents a valuable new biocatalytic tool. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00027863
- Volume :
- 136
- Issue :
- 49
- Database :
- Academic Search Index
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 100326526
- Full Text :
- https://doi.org/10.1021/ja508265b