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NMR–DMF: a modular nuclear magnetic resonance–digital microfluidics system for biological assays.
- Source :
-
Analyst . 2014, Vol. 139 Issue 23, p6204-6213. 10p. - Publication Year :
- 2014
-
Abstract
- We present a modular nuclear magnetic resonance–digital microfluidics (NMR–DMF) system as a portable diagnostic platform for miniaturized biological assays. With increasing number of combinations between designed probes and a specific target, NMR has become an accurate and rapid assay tool, which is capable of detecting particular kinds of proteins, DNAs, bacteria and cells with a customized probe quantitatively. Traditional sample operation (e.g., manipulation and mixing) relied heavily on human efforts. We herein propose a modular NMR–DMF system to allow the electronic automation of multi-step reaction-screening protocols. A figure-8 shaped coil is proposed to enlarge the usable inner space of a portable magnet by 4.16 times, generating a radio frequency (RF) excitation field in the planar direction. By electronically managing the electro-wetting-on-dielectric (EWOD) effects over an electrode array, preloaded droplets with the inclusion of biological constituents and targets can be programmed to mix and be guided to the detection site (3.5 × 3.5 mm2) for high-sensitivity NMR screening (static B field: 0.46 T, RF field: 1.43 mT per ampere), with the result (voltage signal) displayed in real-time. To show the system's utility, automated real-time identification of 100 pM of avidin in a 14 μL droplet was achieved. The system shows promise as a robust and portable diagnostic device for a wide variety of biological analyses and screening applications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NUCLEAR magnetic resonance
*MICROFLUIDICS
*RADIO frequency
*PROTEINS
*DNA
*AVIDIN
Subjects
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 139
- Issue :
- 23
- Database :
- Academic Search Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 100393785
- Full Text :
- https://doi.org/10.1039/c4an01285b