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On-chip self-assembly of cell embedded microstructures to vascular-like microtubes.
- Source :
-
Lab on a Chip . 2014, Vol. 14 Issue 6, p1151-1161. 11p. - Publication Year :
- 2014
-
Abstract
- Currently, research on the construction of vascular-like tubular structures is a hot area of tissue engineering, since it has potential applications in the building of artificial blood vessels. In this paper, we report a fluidic self-assembly method using cell embedded microstructures to construct vascular-like microtubes. A novel 4-layer microfluidic device was fabricated using polydimethylsiloxane (PDMS), which contains fabrication, self-assembly and extraction areas inside one channel. Cell embedded microstructures were directly fabricated using poly(ethylene glycol) diacrylate (PEGDA) in the fabrication area, namely on-chip fabrication. Self-assembly of the fabricated microstructures was performed in the assembly area which has a micro well. Assembled tubular structures (microtubes) were extracted outside the channel into culture dishes using a normally closed (NC) micro valve in the extraction area. The self-assembly mechanism was experimentally demonstrated. The performance of the NC micro valve and embedded cell concentration were both evaluated. Fibroblast (NIH/3T3) embedded vascular-like microtubes were constructed inside this reusable microfluidic device. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14730197
- Volume :
- 14
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Lab on a Chip
- Publication Type :
- Academic Journal
- Accession number :
- 100403961
- Full Text :
- https://doi.org/10.1039/c3lc51134k