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Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the VitekĀ® MS system.

Authors :
Monteiro, Jussimara
Inoue, Fernanda Matsiko
Lobo, Ana Paula T.
Sugawara, Eduardo K.
Boaretti, Fabiana M.
Tufik, Sergio
Source :
Journal of Microbiological Methods. Feb2015, Vol. 109, p157-159. 3p.
Publication Year :
2015

Abstract

A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01677012
Volume :
109
Database :
Academic Search Index
Journal :
Journal of Microbiological Methods
Publication Type :
Academic Journal
Accession number :
100508897
Full Text :
https://doi.org/10.1016/j.mimet.2014.12.009