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Ultra-rapid procedure to test for γ-hydroxybutyric acid in blood and urine by gas chromatography–mass spectrometry
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Jul2003, Vol. 792 Issue 1, p83. 5p. - Publication Year :
- 2003
-
Abstract
- Gamma-hydroxybutyric acid (GHB) is a substance naturally present within mammal species. Properties of a neurotransmitter or neuromodulator are generally suggested for this substance. GHB is therapeutically used as an anaesthetic, but can be used for criminal offences (date-rape drug). It appears that the window of detection of GHB is very short in both blood and urine, and therefore its presence is very difficult to prove after a rape case. Twenty μl of blood or urine were pipetted into a glass tube, followed by 20 μl GHB-d6 and 45 μl acetonitrile. After vortexing and efficient centrifugation, the supernatant was collected and evaporated to dryness. The residue was derivatized with BSTFA+1% TMCS for 20 min at 70 °C. After injection on a 30-m HP5 MS capillary column, GHB (<F>m/z</F> 233, 204 and 147) and GHB-d6 (<F>m/z</F> 239) were identified by mass spectrometry. The procedure was linear from 1 to 200 mg/l for both blood and urine. Precisions were in the range 4 to 11%. The method appears simple, specific and rapid as an accurate result can be obtained within 1 h. [Copyright &y& Elsevier]
- Subjects :
- *GAMMA-hydroxybutyrate
*BLOOD
*URINE
*RAPE
*ACETONITRILE
Subjects
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 792
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 10058612
- Full Text :
- https://doi.org/10.1016/S1570-0232(03)00307-6