Differential expression of DKK1 during early osteogenic and adipogenic differentiation in rat bone marrow mesenchymal stem cells.

Objective To analyzed the DKK1 expression in early osteogenic and adipogenic differentiation of BMSCs in rats and to explore its regulating mechanism. Methods DKK1 gene expression in early adipogenic and osteogenic differentiation was detected using real4ime PCR. And the DKK1 expression was also detected after hormone treatment. Results The expression level of DKK1 gene after 6, 12, 24, and 48h adipogenic induction was higher than that in control group and the difference was significant (P < 0. 01). The expression level of DKK1 gene after 0. 5 and 6h osteogenic induction was obviously lower than that in control group and the difference was significant (P < 0. 05). The expression level of DKK1 gene of BMSCs after 3, 6, 12, and 24 h hormone treatment was higher than in control group (P < 0. 05). Conclusion DKK1 plays an important role in early osteogenic and adipogenic differentiation by BMSCs. The hormone affects osteogenic and adipogenic differentiation by regulating the expression of DKK1. [ABSTRACT FROM AUTHOR]