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Genome-wide study of mRNA degradation and transcript elongation in Escherichia coli.
- Source :
-
Molecular Systems Biology . Jan2015, Vol. 11 Issue 1, pn/a-N.PAG. 10p. - Publication Year :
- 2015
-
Abstract
- An essential part of gene expression is the coordination of RNA synthesis and degradation, which occurs in the same cellular compartment in bacteria. Here, we report a genome-wide RNA degradation study in Escherichia coli using RNA-seq, and present evidence that the stereotypical exponential RNA decay curve obtained using initiation inhibitor, rifampicin, consists of two phases: residual RNA synthesis, a delay in the interruption of steady state that is dependent on distance relative to the mRNA's 5′ end, and the exponential decay. This gives a more accurate RNA lifetime and RNA polymerase elongation rate simultaneously genome-wide. Transcripts typically have a single RNA decay constant along all positions, which is distinct between different operons, indicating that RNA stability is unlikely determined by local sequences. These measurements allowed us to establish a model for RNA processing involving co-transcriptional degradation, providing quantitative description of the macromolecular coordination in gene expression in bacteria on a system-wide level. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENOMICS
*MOLECULAR genetics
*MESSENGER RNA
*RNA
*ESCHERICHIA coli
Subjects
Details
- Language :
- English
- ISSN :
- 17444292
- Volume :
- 11
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Molecular Systems Biology
- Publication Type :
- Academic Journal
- Accession number :
- 100711574
- Full Text :
- https://doi.org/10.15252/msb.20145794