Identification of the MinimumPeptide from Mouse MyostatinProdomain for Human Myostatin Inhibition.

Myostatin,an endogenous negative regulator of skeletal musclemass, is a therapeutic target for muscle atrophic disorders. Here,we identified minimum peptides 2and 7toeffectively inhibit myostatin activity, which consist of 24 and 23amino acids, respectively, derived from mouse myostatin prodomain.These peptides, which had the propensity to form α-helix structure,interacted to myostatin with KDvaluesof 30–36 nM. Moreover, peptide 2significantlyincreased muscle mass in Duchenne muscular dystrophy model mice. [ABSTRACT FROM AUTHOR]