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Downregulating galectin-3 inhibits proinflammatory cytokine production by human monocyte-derived dendritic cells via RNA interference.
- Source :
-
Cellular Immunology . Mar2015, Vol. 294 Issue 1, p44-53. 10p. - Publication Year :
- 2015
-
Abstract
- Galectin-3 (Gal-3), a β-galactoside-binding lectin, serves as a pattern-recognition receptor (PRR) of dendritic cells (DCs) in regulating proinflammatory cytokine production. Galectin-3 (Gal-3) siRNA downregulates expression of IL-6, IL-1β and IL-23 p19, while upregulates IL-10 and IL-12 p35 in TLR/NLR stimulated human MoDCs. Furthermore, Gal-3 siRNA-treated MoDCs enhanced IFN-γ production in SEB-stimulated CD45RO CD4 T-cells, but attenuated IL-17A and IL-5 production by CD4 T-cells. Addition of neutralizing antibodies against Gal-3, or recombinant Gal-3 did not differentially modulate IL-23 p19 versus IL-12 p35. The data indicate that intracellular Gal-3 acts as cytokine hub of human DCs in responding to innate immunity signals. Gal-3 downregulation reprograms proinflammatory cytokine production by MoDCs that inhibit Th2/Th17 development. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00088749
- Volume :
- 294
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cellular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 101062244
- Full Text :
- https://doi.org/10.1016/j.cellimm.2015.01.017