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Discovery of a Heparan Sulfate 3-O-Sulfation Specific Peeling Reaction.
- Source :
-
Analytical Chemistry . 1/6/2015, Vol. 87 Issue 1, p592-600. 9p. - Publication Year :
- 2015
-
Abstract
- Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection. However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above. By contrast, multiple distinct isoforms of 3-O-sulfotranserases exist in mammals (up to seven isoenzymes). Here we describe a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end. When HS/heparin is enzymatically depolymerized for compositional analysis, 3-O-sulfated glucosamine at the reducing ends appears to be susceptible to degradation under mildly basic conditions. We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed. Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEPARAN sulfate
*POLYSACCHARIDES
*SULFATION
*HEPARIN
*HERPES simplex
*GLUCOSAMINE
Subjects
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 87
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 101080159
- Full Text :
- https://doi.org/10.1021/ac503248k