Back to Search
Start Over
Reaction-based epoxide fluorescent probe for in vivo visualization of hydrogen sulfide.
- Source :
-
Biosensors & Bioelectronics . Jun2015, Vol. 68, p681-687. 7p. - Publication Year :
- 2015
-
Abstract
- Hydrogen sulfide (H 2 S) has emerged as the most important biosynthetic gasotransmitters along with nitric oxide (NO) and carbon monoxide (CO). In this study, we report the design and the synthesis of a new epoxide fluorescent probe 7-glycidyloxy-9-(2-glycidyloxycarbonylphenyl)-2-xanthone (FEPO) for use in in vivo visualization of hydrogen sulfide. The probe employs a fluorescein as a fluorophore, and is equipped with an operating epoxide unit. FEPO functions via epoxide ring opening upon nucleophilic attack of H 2 S. This ring opening strategy may open a new avenue for the development of various H 2 S fluorescent sensors. FEPO showed high selectivity and high sensitivity for H 2 S. FEPO's cytotoxicity was tested using MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide) assay. Furthermore, the use of confocal imaging of H 2 S and in vivo imaging in live zebra fish demonstrated FEPO's potential biological applications. We anticipate that, owing to their ideal properties, probes of this type will find great uses in exploring the role of H 2 S in biology. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 68
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 101342453
- Full Text :
- https://doi.org/10.1016/j.bios.2015.01.070