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Ginsenoside-Rb2 Inhibits Dexamethasone-Induced Apoptosis Through Promotion of GPR120 Induction in Bone Marrow-Derived Mesenchymal Stem Cells.
- Source :
-
Stem Cells & Development . Mar2015, Vol. 24 Issue 6, p781-790. 10p. - Publication Year :
- 2015
-
Abstract
- Apoptosis of bone marrow-derived mesenchymal stem cells (BMMSCs) is an essential pathogenic factor of osteoporosis. Ginsenoside-Rb2 (Rb2), a 20(S)-protopanaxadiol glycoside extracted from ginseng, is a potent treatment for bone loss, which raises interest regarding the bone metabolism area. In the present study, we found that dose-response Rb2 inhibited high dosage of dexamethasone (Dex)-induced apoptosis in primary murine BMMSCs. Interestingly, Rb2 promoted GPR120 induction, which is the unsaturated long-chain fatty acid receptor. We further confirmed that GPR120-specific ShRNA reversed the inhibition of Rb2 on Dex-induced apoptosis by activating caspase-3 and reducing cell viability. In addition, Rb2 notably increased phosphorylated ERK1/2 levels and Ras kinase activity dependently through the GPR120. The ERK1/2 activity-specific inhibitor U0126 remarkably blocked the Rb2-induced antiapoptotic effect in response to Dex-induced apoptosis. Together, dose-response Rb2 protected BMMSCs against Dex-induced apoptosis dependently by inducing GPR120 promoted Ras-ERK1/2 signaling pathway. Therefore, in the prevalence of the abuse of Dex in the clinic, our findings suggest for the first time that Rb2 is not only a key to understand the link between Chinese medicine and the pathology of osteoporosis but also an underlying target for the treatment of bone complications in the foreseeable future. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15473287
- Volume :
- 24
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Stem Cells & Development
- Publication Type :
- Academic Journal
- Accession number :
- 101421613
- Full Text :
- https://doi.org/10.1089/scd.2014.0367