Back to Search
Start Over
Capsaicin-mediated apoptosis of human bladder cancer cells activates dendritic cells via CD91.
- Source :
-
Nutrition . Apr2015, Vol. 31 Issue 4, p578-581. 4p. - Publication Year :
- 2015
-
Abstract
- Objectives: Immunostimulation by anticancer cytotoxic drugs is needed for long-term therapeutic success. Activation of dendritic cells (DCs) is crucial to obtain effective and long-lasting anticancer T-cell mediated immunity. The aim of this study was to explore the effect of capsaicin-mediated cell death of bladder cancer cells on the activation of human monocyte-derived CD1a+ immature DCs. Methods: Immature DCs (generated from human peripheral blood-derived CD14+ monocytes cultured with granulocyte-macrophage colony stimulating factor and interleukin-4) were cocultured with capsaicin (CPS)-induced apoptotic bladder cancer cells. DC activation was investigated using immunofluorescence and flow cytometric analysis for key surface molecules. In some experiments, CD91 was silenced in immature DCs. Results: We found that capsaicin-mediated cancer cell apoptosis upregulates CD86 and CD83 expression on DCs, indicating the induction of DC activation. Moreover, silencing of CD91 (a common receptor for damage-associated molecular patterns, such as calreticulin and heat-shock protein-90/70) in immature DCs led to the inhibition of DC activation. Conclusions: Our data show that CPS-mediated cancer cell apoptosis activates DCs via CD91, suggesting CPS as an attractive candidate for cancer therapy [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08999007
- Volume :
- 31
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 101674779
- Full Text :
- https://doi.org/10.1016/j.nut.2014.05.005