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Quantitative analysis of the eight major compounds in the Samsoeum using a high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometer.
- Source :
-
Pharmacognosy Magazine . Apr-Jun2015, Vol. 11 Issue 42, p320-326. 7p. - Publication Year :
- 2015
-
Abstract
- Background: Samsoeum was traditionally used for treatment of a respiratory disease. Objective: The simultaneous determination of eight major compounds, ginsenoside Rg3, caffeic acid, puerarin, costunolide, hesperidin, naringin, glycyrrhizin, and 6-gingerol in the Samsoeum using a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) and an electrospray ionization mass spectrometer was developed for an accurate and reliable quality assessment. Materials and Methods: Eight compounds were qualitative identified based on their mass spectra and by comparing with standard compounds and quantitative analyzed by HPLC-DAD. Separation of eight compounds was carried out on a LUNA C18 column (S-5 μm, 4.6 mm i.d. ×250 mm) with gradient elution composed of acetonitrile and 0.1% trifluoroacetic acid. Results: The data showed good linearity (R2 > 0.9996). The limits of detection and the limits of quantification were <0.53 μg and 1.62 μg, respectively. Inter-.and Intra-day precisions (expressed as relative standard deviation values) were within 1.94% and 1.91%, respectively. The recovery of the method was in the range of 94.24-107.90%. Conclusion: The established method is effective and could be applied to quality control of Samsoeum. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09731296
- Volume :
- 11
- Issue :
- 42
- Database :
- Academic Search Index
- Journal :
- Pharmacognosy Magazine
- Publication Type :
- Academic Journal
- Accession number :
- 101746086
- Full Text :
- https://doi.org/10.4103/0973-1296.153085