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Preparation of Flavocoenzyme Isotopologues by Biotransformation of Purines.
- Source :
-
Journal of Organic Chemistry . 3/6/2015, Vol. 80 Issue 5, p2539-2544. 6p. - Publication Year :
- 2015
-
Abstract
- Isotope-labeled flavins are crucial reporters for many biophysical studies of flavoproteins. A purine-deficient Escherichia coli strain engineered for expression of the ribAGH genes of Bacillus subtilis converts isotope-labeled purine supplements into the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, with yields up to 40%. The fermentation products can subsequently be converted into isotope-labeled riboflavin and the cognate flavocoenzymes, FMN and FAD, by in vitro biotransformation with better than 90% yield. Using this approach, more than 100 single or multiple 13C-, 15N-, 17O-, and 18O-labeled isotopologues of these cofactors and ligands become easily accessible, enabling advanced ligand-based spectroscopy of flavoproteins and lumazine receptor proteins at unprecedented resolution. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLAVINS
*PHYSICAL biochemistry
*FLAVOPROTEINS
*VITAMIN B2
*FERMENTATION
Subjects
Details
- Language :
- English
- ISSN :
- 00223263
- Volume :
- 80
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Organic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 101785875
- Full Text :
- https://doi.org/10.1021/jo502480w