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The phasin PhaF controls bacterial shape and size in a network-forming strain of Pseudomonas putida.

The phasin PhaF controls bacterial shape and size in a network-forming strain of Pseudomonas putida.

Authors :
Obeso, José I.
Gómez-Botrán, José L.
Olivera, Elías R.
Luengo, José M.
Source :
Journal of Biotechnology. Apr2015, Vol. 199, p17-20. 4p.
Publication Year :
2015

Abstract

Pseudomonas putida N, a poly-3-hydroxyalkonate (PHA)-producing bacterium, showing ampicillin resistance, is an unusual strain. In the presence of this antibiotic, it grows as giant cells (25–50 μm) forming complex networks inter-connected by micro-tubular structures. The transformation of this bacterium with a plasmid containing the gene phaF , which encodes a phasin involved in the molecular architecture of the PHA-granules, (i) restores the wild-type phenotype by reducing both bacterial size and length (coco-bacilli ranging between 0.5 and 3 μm), and (ii) increases ampicillin resistance by more than 100-fold. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01681656
Volume :
199
Database :
Academic Search Index
Journal :
Journal of Biotechnology
Publication Type :
Academic Journal
Accession number :
101919089
Full Text :
https://doi.org/10.1016/j.jbiotec.2015.01.022