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MicroRNA-181a is involved in the regulation of human endometrial stromal cell decidualization by inhibiting Krüppel-like factor 12.
- Source :
-
Reproductive Biology & Endocrinology . 2015, Vol. 13 Issue 1, p1-9. 9p. - Publication Year :
- 2015
-
Abstract
- Background: The transformation of endometrium into decidua is essential for normal implantation of the blastocyst. However, the post-transcriptional regulation and the miRNAs involved in decidualization remain poorly understood. Here, we examined microRNA-181a (miR-181a) expression in decidualized human endometrial stromal cell (hESC). In addition, we investigated the functional effect of miR-181a on hESC decidualization in vitro. Methods: Quantitative real-time PCR (qRT-PCR) was used to detect the profile of miR-181a in decidualized hESC. qRT-PCR, enzyme-linked fluorescent assay, and immunofluorescence assay were performed to investigate decidualization marker genes' expression after enhancing or inhibition of miR-181a expression in hESC. Luciferase reporter assay, western blotting, qRT-PCR, and immunofluorescence assay were carried out to identify the relationship between miR-181a and Krüppel-like factor 12 (KLF12). Results: miR-181a expression levels increased dramatically in hESC treated with 8-Br-cAMP and MPA. Increased miR-181a expression promoted hESC decidualization-related gene expression and morphological transformation; conversely, inhibition of miR-181a expression compromised hESC decidualization in vitro. Further analysis confirmed that miR-181a interacted with the 3' untranslated region of the transcription factor KLF12 and down-regulated KLF12 at the transcriptional and translational levels. KLF12 overexpression abolished miR-181a-induced decidualization. Conclusions: Our findings suggest that miR-181a plays a functionally important role in human endometrial stromal cell decidualization in vitro by inhibiting KLF12. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*CONNECTIVE tissue cells
*STROMAL cells
*RNA
*MESENCHYMAL stem cells
Subjects
Details
- Language :
- English
- ISSN :
- 14777827
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Reproductive Biology & Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 101990741
- Full Text :
- https://doi.org/10.1186/s12958-015-0019-y